| 出品公司: | ATCC |
|---|---|
| 载体名称: | pACYC177 |
| 质粒类型: | 大肠杆菌克隆载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3941bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | 37031 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
pACYC177载体质粒基本信息
pACYC177质粒图谱载体图谱和pACYC177载体序列质粒序列多克隆位点信息
pACYC177质粒载体简介
pACYC177 is an E. coli plasmid cloning vector containing the p15A origin of replication (1-4). This allows pACYC177 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell (5), but can be amplified with chloramphenicol or spectinomycin.
Enzymes with unique restriction sites are shown in bold type and enzymes with two restriction sites are shown in regular type. Location of sites of all NEB restriction enzymes can be found on the NEB web site (choose Technical Reference > DNA Sequences and Maps). Restriction site coordinates refer to the position of the 5′-most base on the top strand in each recognition sequence.
pACYC177质粒序列载体序列
LOCUS pACYC177 3941 bp DNA SYN
DEFINITION pACYC177
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3941
/organism="pACYC177"
/mol_type="other DNA"
gene 10..618
/label="Ampicillin (252 - 860)"
/gene="Ampicillin (252 - 860)"
CDS 100..618
/label="ORF frame 1"
/translation="MTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVT
RLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAG
PLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQ
IAEIGASLIKHW*"
rep_origin complement(767..1595)
/label="p15A_origin"
/translation="MTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVT
RLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAG
PLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQ
IAEIGASLIKHW*"
gene 1923..2738
/label="KanR2"
/gene="KanR2"
/translation="MTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVT
RLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAG
PLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQ
IAEIGASLIKHW*"
CDS 1923..2738
/label="ORF frame 3"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
misc_feature 3025..3515
/label="Tn3R"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
promoter 3629..3657
/label="AmpR_promoter"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
gene 3699..618
/label="Ampicillin"
/gene="Ampicillin"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
CDS 3699..618
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
61 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
301 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
481 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct
781 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
841 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
901 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
961 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
1021 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
1081 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
1141 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
1201 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
1261 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
1321 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
1381 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
1441 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
1501 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
1561 ccaacatagt aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg
1621 ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac
1681 ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca
1741 cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
1801 gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
1861 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
1921 ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg
1981 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
2041 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
2101 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
2161 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
2221 cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
2281 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
2341 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
2401 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
2461 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
2521 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
2581 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
2641 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
2701 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
2761 aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa taaatcgaac
2821 ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc
2881 aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg
2941 ctccctcact ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc
3001 ttcttcacga ggcagacctc agcgctcaaa gatgcagggg taaaagctaa ccgcatcttt
3061 accgacaagg catccggcag ttcaacagat cgggaagggc tggatttgct gaggatgaag
3121 gtggaggaag gtgatgtcat tctggtgaag aagctcgacc gtcttggccg cgacaccgcc
3181 gacatgatcc aactgataaa agagtttgat gctcagggtg tagcggttcg gtttattgac
3241 gacgggatca gtaccgacgg tgatatgggg caaatggtgg tcaccatcct gtcggctgtg
3301 gcacaggctg aacgccggag gatcctagag cgcacgaatg agggccgaca ggaagcaaag
3361 ctgaaaggaa tcaaatttgg ccgcaggcgt accgtggaca ggaacgtcgt gctgacgctt
3421 catcagaagg gcactggtgc aacggaaatt gctcatcagc tcagtattgc ccgctccacg
3481 gtttataaaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
3541 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
3601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
3661 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
3721 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
3781 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
3841 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
3901 gcacttttaa agttctgcta tgtggcgcgg tattatcccg t
//
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