| 质粒名称: | RCAS GFP (CT#22) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 13878 |
| 存储实验室: | Connie Cepko |
|
载体骨架基本信息 |
|
| 载体名称: | RCAS (A) |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 11600 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 逆转录病毒；禽表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GFP |
| 物种来源: | -- |
| 插入大小: | 700 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | ClaI(未破坏) |
| 3' 克隆位点: | ClaI（克隆过程中被破坏） |
| 5' 测序引物 | RCAS-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | RCAS GFP (CT#22) was a gift from Connie Cepko (Addgene plasmid # 13878 ; http://n2t.net/addgene:13878 ; RRID:Addgene_13878) |
| 参考文献部分: | Production and design of more effective avian replication-incompetent retroviral vectors. Chen CM, Smith DM, Peters MA, Samson ME, Zitz J, Tabin CJ, Cepko CL. Dev Biol. 1999 Oct 15. 214(2):370-84. 10.1006/dbio.1999.9432 PubMed 10525341 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS RCAS GFP (CT22) 12448 bp ds-DNA circular SYN 19-MAR-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS RCAS GFP (CT22)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12448)
AUTHORS Chen CM, Smith DM, Peters MA, Samson ME, Zitz J, Tabin CJ, Cepko CL
TITLE Production and design of more effective avian
replication-incompetent retroviral vectors.
JOURNAL Dev Biol. 1999 Oct 15. 214(2):370-84.
PUBMED 10525341
REFERENCE 2 (bases 1 to 12448)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 19, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12448
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(623..645)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 634..650
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(658..688)
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
primer_bind complement(731..749)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 817..921
/gene="bla"
/label=AmpR promoter
CDS 922..1782
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1140..1159)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1953..2541
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2442..2461
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2695..2712
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 2727..2867
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(2793..2812)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2953..2975
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(2969..3160)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
promoter 4003..4264
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
CDS 11312..12031
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(11357..11378)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(11618..11637)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 11965..11986
/label=EGFP-C
/note="EGFP, forward primer"
promoter 12220..33
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
ORIGIN
1 gccatttgac cattcaccac attggtgtgc acctgggttg atggccggac cgttgattcc
61 ctgacgacta cgagcacctg catgaagcag aaggcttcat ttggtgaccc cgacgtgata
121 gttagggaat agtggtcggc cacagacggc gtggcgatcc tgtctccatc cgtctcgtct
181 atcgggaggc gagttcgatg accctggtgg agggggctgc ggcttaggga ggcagaagct
241 gagtaccgtc ggaggggatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
301 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
361 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
421 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
481 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
541 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt
601 aaagctccaa gcttggctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacat
661 tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gaattcttga
721 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
781 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
841 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
901 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
961 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
1021 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
1081 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
1141 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
1201 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
1261 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
1321 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
1381 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
1441 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
1501 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
1561 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
1621 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
1681 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
1741 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
1801 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
1861 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
1921 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
1981 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
2041 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
2101 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
2161 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
2221 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
2281 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
2341 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
2401 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
2461 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
2521 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
2581 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
2641 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
2701 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
2761 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
2821 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
2881 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
2941 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
3001 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct
3061 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
3121 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaaggag
3181 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
3241 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
3301 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag
3361 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg
3421 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc
3481 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta
3541 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg
3601 acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg
3661 tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat
3721 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
3781 ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc
3841 ggggaggcag acaaggtata gggcggcgat gcgatgtacg ggccagatat acgcgtatct
3901 gaggggacta gggtgtgttt aggcgaaaag cggggcttcg gttgtacgcg gttaggagtc
3961 ccctcaggat atagtagttt cgcttttgca tagggagggg gaaatgtagt cttatgcaat
4021 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
4081 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
4141 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttccgcattg cagagatatt
4201 gtatttaagt gcctagctcg atacaataaa cgccatttga ccattcacca cattggtgtg
4261 cacctgggtt gatggccgga ccgttgattc cctgacgact acgagcacct gcatgaagca
4321 gaaggcttca tttggtgacc ccgacgtgat agttagggaa tagtggtcgg ccacagacgg
4381 cgtggcgatc ctgtctccat ccgtctcgtc tatcgggagg cgagttcgat gaccctggtg
4441 gagggggctg cggcttaggg aggcagaagc tgagtaccgt cggagggagc tccagggccc
4501 ggagcgactg acccctgccg agaactcaga gggtcgtcgg aagacggaga gtgagcccga
4561 cgaccacccc aggcacgtct ttggtcggcc tgcggatcaa gcatggaagc cgtcattaag
4621 gtgatttcgt ccgcgtgtaa aacctattgc gggaaaatct ctccttctaa gaaggaaata
4681 ggggccatgt tgtccctgtt acaaaaggaa gggttgctta tgtctccctc agatttatat
4741 tctccggggt cctgggatcc catcactgcg gcgctctccc agcgggcaat ggtacttgga
4801 aaatcgggag agttaaaaac ctggggattg gttttggggg cattgaaggc ggctcgagag
4861 gaacaggtta catctgagca agcaaagttt tggttgggat tagggggagg gagggtctct
4921 cccccaggtc cggagtgcat cgagaaacca gctacggagc ggcgaatcga caaaggggag
4981 gaggtgggag aaacaactgt gcagcgagat gcgaagatgg cgccagagga agcggccaca
5041 cctaaaaccg ttggcacatc ctgctatcat tgcggaacag ctgttggctg caattgcgcc
5101 accgccacag cctcggcccc tcctccccct tatgtgggga gtggtttgta tccttccctg
5161 gcgggggtgg gagagcagca gggccaggga gataacacgt ctcggggggc ggagcagcca
5221 agggaggagc cagggcacgc gggtcaggcc cctgggccgg ccctgactga ctgggcaagg
5281 gtaagggagg agcttgcgag tactggtccg cccgtggtgg ccatgcctgt agtgattaag
5341 acagagggac ccgcctggac ccctctggag ccaaaattga tcacaagact ggctgatacg
5401 gtcaggacca agggcttacg atccccgatc actatggcag aagtggaagc gctcatgtcc
5461 tccccgttgc tgccgcatga cgtcacgaat ctaatgagag tgattttagg acctgcccca
5521 tatgccttat ggatggacgc ttggggagtc caactccaga cggttatagc ggcagccact
5581 cgcgaccccc gacacccagc gaacggtcaa gggcgggggg aacggactaa cttggatcga
5641 ttaaagggct tagctgatgg gatggtgggc aacccacagg gtcaggccgc attattaaga
5701 ccgggggaat tggttgctat tacggcgtcg gctctccagg cgtttagaga agttgcccgg
5761 ctggcggaac ctgcaggtcc atgggcggac atcacgcagg gaccatctga gtcctttgtt
5821 gattttgcca atcggcttat aaaggcggtt gaggggtcag atctcccgcc ttccgcgcgg
5881 gctccggtga tcattgactg ctttaggcag aagtcacagc cagatattca gcagcttata
5941 cgggcagcac cctccacgct gaccacccca ggagagataa tcaaatatgt gctagacagg
6001 cagaagattg cccctcttac ggatcaaggc atagccgcgg ccatgtcgtc tgctatccag
6061 cccttagtta tggcagtagt caatagagag agggatggac aaactgggtc gggtggtcgt
6121 gcccgagggc tctgctacac ttgtggatcc ccgggacatt atcaggcaca gtgcccgaaa
6181 aaacgaaagt caggaaacag ccgtgagcga tgtcagctgt gtgacgggat gggacacaac
6241 gctaaacagt gtaggaagcg ggatggcaac cagggccaac gcccaggaag aggtctctct
6301 tcggggccgt ggcccggccc tgagcagcct gccgtctcgt tagcgatgac aatggaacat
6361 aaagatcgcc ccttggttag ggtcattctg actaacactg ggagtcatcc agtcaaacaa
6421 cgttcggtgt atatcaccgc gctgttggac tccggagcgg acatcactat tatttcggag
6481 gaggattggc ctactgattg gccggtggtg gacaccgcga acccacagat ccatggcata
6541 ggagggggaa ttcccatgcg aaaatcccgg gatatgatag aggtgggggt tattaaccga
6601 gacgggtcgt tggagcgacc cctgctcctc ttccccgcag tcgctatggt tagagggagt
6661 atcctaggaa gagattgtct gcagggccta gggctccgct tgacaaattt atagggaggg
6721 ccactgttct tactgttgcg ctacatctgg ctattccgct caaatggaag tcagaccgca
6781 cgcctgtgtg gattgaccag tggccccttc ctgaaggtaa acttgtagcg ctaacgcaat
6841 tagtggaaaa agaattacag ttaggacata tagagccctc acttagttgt tggaacacac
6901 ctgtttttgt gatccggaag gcttccgggt cttatcgctt attgcatgat ttgcgcgctg
6961 ttaacgccaa gcttgttcct tttggggccg tccaacaggg ggcgccagtt ctctccgcgc
7021 tcccgcgtgg ctggcccctg atggtcctag acctcaagga ttgcttcttt tctatccctc
7081 ttgcggaaca agatcgcgaa gcttttgcat ttacgctccc ctctgtgaat aaccaggccc
7141 ccgctcgaag attccaatgg aaggtcttgc cccaagggat gacctgttct cccactatct
7201 gtcagttggt agtgggtcag gtgctcgagc ccttgcgact caagcaccca gctctgcgca
7261 tgttgcatta tatggacgat cttttgctag ccgcctcaag tcatgatggg ttggaagcgg
7321 cagggaagga ggttatcggt acattggaaa gagccgggtt cactatttcg ccggataaga
7381 tccagaggga gcccggagta caatatcttg ggtacaagtt aggcagtacg tatgtagcac
7441 ccgtaggctt ggtagcagaa cccaggatag ccaccttgtg ggatgttcaa aagctggtgg
7501 ggtcacttca gtggcttcgc ccagcgttag ggatcccgcc acgactgatg ggtccctttt
7561 atgagcagtt acgagggtca gatcctaacg aggcgaggga atggaatcta gacatgaaaa
7621 tggcctggag agagatcgta cagcttagca ctactgctgc cttggaacga tgggaccctg
7681 cccagcctct ggaaggagcg gtcgctagat gtgaacaggg ggcaataggg gtcctgggac
7741 agggactgtc cacacaccca aggccatgtt tgtggttatt ctccacccaa cccaccaagg
7801 cgtttactgc ttggttagaa gtgctcaccc ttttgattac taagctacgc gcttcggcag
7861 tgcgaacctt tggcaaggag gttgatatcc tcctgttgcc tgcatgcttc cgggaggacc
7921 ttccgctccc ggaggggatc ctgttagcac ttagggggtt tgcaggaaaa atcaggagta
7981 gtgacacgcc atctattttt gacattgcgc gtccactgca tgtttctctg aaagtgaggg
8041 ttaccgacca ccctgtgccg ggacccactg tctttaccga cgcctcctca agcacccata
8101 aaggggtggt agtctggagg gagggcccaa ggtgggagat aaaagaaata gttgatttgg
8161 gggcaagtgt acaacaactg gaggcacgcg ctgtggccat ggcacttctg ctgtggccga
8221 caacgcccac taatgtagtg actgactctg cgtttgttgc gaaaatgtta ctcaagatgg
8281 gacaggaggg agtcccgtct acagcggcag cttttatttt agaggatgcg ttaagccaaa
8341 ggtcagccat ggccgccgtt ctccacgtgc ggagtcattc tgaagtgcca gggtttttca
8401 cagaaggaaa tgacgtggca gatagccaag ccacctttca agcgtatccc ttgagagagg
8461 ctaaagatct tcataccgct ctccatattg gaccccgcgc gctatccaaa gcgtgtaata
8521 tatctatgca gcaggctagg gaggttgttc agacctgccc gcattgtaat tcagcccctg
8581 cgttggaggc cggggtaaac cctaggggtt tgggacccct acagatatgg cagacagact
8641 ttacgcttga gcctagaatg gctccccgtt cctggctcgc tgttactgtg gacaccgcct
8701 catcagcgat agtcgtaact cagcatggcc gtgttacatc ggttgctgca caacatcatt
8761 gggccacggc tatcgccgtt ttgggaagac caaaggccat aaaaacagat aacgggtcct
8821 gcttcacgtc taaatccacg cgggagtggc tcgcgagatg ggggatagca cacaccaccg
8881 ggattccggg aaattcccag ggtcaagcta tggtagagcg ggccaaccgg ctcctgaaag
8941 ataagatccg tgtgcttgcg gaaggggacg gctttatgaa aagaatcccc gccagcaaac
9001 agggggaact actagccaaa gcaatgtatg ccctcaatca ctttgagcgt ggtgaaaaca
9061 caaaaacacc ggtacaaaaa cactggagac ctaccgttct tacagaagga cccccggtta
9121 aaatacgaat agagacaggg gagtgggaaa aaggatggaa cgtgctggtc tggggacgag
9181 gttatgccgc tgtgaaaaac agggacactg ataaggttat ttgggtaccc tctcggaaag
9241 ttaaaccgga tgtcacccaa aaggatgagg tgactaagaa agatgaggcg agccctcttt
9301 ttgcaggcat ttctgactgg ataccctggg aagacgagca agaaggactc caaggagaaa
9361 ccgctagcaa caagcaagaa agacccggag aagacaccct tgctgccaac gagagttaat
9421 tatattctca ttattggtgt cctggtcttg tgtgaggtta cgggggtaag agctgatgtc
9481 cacttactcg agcagccagg gaacctttgg attacatggg ccaaccgtac aggccaaacg
9541 gatttttgcc tctctacaca gtcagccacc tccccttttc aaacatgttt gataggtatc
9601 ccgtccccta tttccgaggg tgattttaag ggatatgttt ctgatacaaa ttgcaccacc
9661 ttgggaactg atcggttagt ctcgtcagcc gactttactg gcggacctga caacagtacc
9721 accctcactt atcggaaggt ctcatgcttg ttgttaaagc tgaatgtctc tatgtgggat
9781 gagccacctg aactacagct gttaggttcc cagtctctcc ctaacattac taatattgct
9841 cagatttccg gtataaccgg gggatgcgta ggcttcagac cacaaggggt tccttggtat
9901 ctaggttggt ctagacagga ggccacgcgg tttctcctta gacacccctc tttctctaaa
9961 tccacggaac cgtttacggt ggtgacagcg gataggcaca atctttttat ggggagtgag
10021 tactgcggtg catatggcta cagattttgg aacatgtata actgctcaca ggtggggcgg
10081 cagtaccgct gtggtaatgc gcgcagcccc cgcccgggtc ttcctgaaat ccagtgtaca
10141 aggagaggag gcaaatgggt taatcaatca caggaaatta atgagtcgga gccgttcagc
10201 tttacggtga actgtacagc tagtagtttg ggtaatgcca gtgggtgttg cggaaaagca
10261 ggcacgattc tcccgggaaa gtgggtcgac agcacacaag gtagtttcac caaaccaaaa
10321 gcgctaccac ccgcaatttt cctcatttgt ggggatcgcg catggcaagg aattcccagt
10381 cgtccggtag ggggcccctg ctatttaggc aagcttacca tgttagcacc taagcataca
10441 gatattctca aggtgcttgt caattcatcg cggacaggta taagacgtaa acgaagcacc
10501 tcacacctgg atgatacatg ctcagatgaa gtgcagcttt ggggtcctac agcaagaatc
10561 tttgcatcta tcctagcccc gggggtagca gctgcgcaag ccttaagaga aattgagaga
10621 ctagcctgtt ggtccgttaa acaggctaac ttgacaacat cactcctcgg ggacttattg
10681 gatgatgtca cgagtattcg acacgcggtc ctgcagaacc gagcggctat tgacttcttg
10741 ctcctagctc acggccatgg ctgtgaggac gttgccggaa tgtgctgttt caatttgagt
10801 gatcacagtg agtctataca gaagaagttc cagctaatga aggaacatgt caataagatc
10861 ggcgtggaca gcgacccaat tggaagttgg ctgcgaggac tattcggggg aataggagaa
10921 tgggccgttc atttgctgaa aggactgctt ttggggcttg tagttatttt gttgctagta
10981 gtgtgcctgc cttgcctttt gcaaatcgta tgcggtaaca tcagaaagat gattaataac
11041 tccatcagct accacacgga atataagaag ctgcaaaagg cctgtgggca gcctgaaagc
11101 agaatagtat aaggcagtac atgggtggtg gtatagcgct tgcgagtcca tcgaggaagg
11161 caggaaagac agctattggt aattgtgaaa tacgcttttg tctgtgtgct gcaggagctg
11221 agctgactct gctggtggcc tcgcgtacca ctgtggcatc gattctagac cactgtggcc
11281 aggcggtagc tgggacgtgc agccgaccac catggtgagc aagggcgagg agctgttcac
11341 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
11401 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
11461 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
11521 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
11581 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
11641 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
11701 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
11761 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
11821 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
11881 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
11941 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
12001 cactctcggc atggacgagc tgtacaagta aagcggccgc gactctaggg gatcctctag
12061 agtcgacctg cagccaagct tatcgatgcg atgtacgggc cagatatacg cgtatctgag
12121 gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc
12181 tcaggatata gtagtttcgc ttttgcatag ggagggggaa atgtagtctt atgcaatact
12241 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
12301 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
12361 acagacgggt ctgacatgga ttggacgaac cactgaattc cgcattgcag agatattgta
12421 tttaagtgcc tagctcgata caataaac
//
