| 质粒名称: | pSB2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 82026 |
| 存储实验室: | Victor Sourjik |
|
载体骨架基本信息 |
|
| 载体名称: | pKG116 |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 4211 bp |
| 完整质粒大小: | 6087 bp |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | 编码大肠杆菌Tar和枯草杆菌McpC的杂交受体：McpC[1-286]-Tar[200-553] |
| 物种来源: | E. coli, B. subtilis |
| 插入大小: | 1923 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NdeI(未破坏) |
| 3' 克隆位点: | BamHI(未破坏) |
| 5' 测序引物 | CGGGCGCAATATTCATGTTG |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pSB2 was a gift from Victor Sourjik (Addgene plasmid # 82026 ; http://n2t.net/addgene:82026 ; RRID:Addgene_82026) |
| 参考文献部分: | Engineering Hybrid Chemotaxis Receptors in Bacteria. Bi S, Pollard AM, Yang Y, Jin F, Sourjik V. ACS Synth Biol. 2016 Jun 10. 10.1021/acssynbio.6b00053 PubMed 27285081 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSB2 6087 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Hybrid McpC[1-286]-Tar[200-553] expression plasmid.
ACCESSION .
VERSION .
KEYWORDS pSB2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6087)
AUTHORS Bi S, Pollard AM, Yang Y, Jin F, Sourjik V
TITLE Engineering Hybrid Chemotaxis Receptors in Bacteria.
JOURNAL ACS Synth Biol. 2016 Jun 10.
PUBMED 27285081
REFERENCE 2 (bases 1 to 6087)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6087
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 324..358
/label=lambda t0 terminator
/note="minimal transcription terminator from phage lambda
(Scholtissek and Grosse, 1987)"
CDS complement(777..1436)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 1351..1370
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(1437..1539)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(1931..1948)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2065..2610)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(2145..2164)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cgatcaagtc gcattggcgg tttcggaaat ggatcgcgtc acgcaacaga acgcatcgct
61 ggtgcaggaa tcagctgccg ccgccgctgc gctggaagaa caggcgagtc gtttaacgca
121 agcagtttcc gcgttccgtc tggcagccag cccactcacc aataaaccgc aaacaccatc
181 ccgtcctgcc agtgagcaac caccggctca gccacgactg cgaattgctg aacaagatcc
241 aaactgggaa acattttgag gatccgcgga taaataagta acgatccggt ccagtaatga
301 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
361 gtgagaatcg cagcaacttg tcgcgccaat cgagccatgt cgtcgtcaac gaccccccat
421 tcaagaacag caagcagcat tgagaacttt ggaatccagt ccctcttcca cctgctgacc
481 ggatcagcag tccccggaac atcgtagctg acgccttcgc gttgctcagt tgtccaaccc
541 cggaaacggg aaaaagcaag ttttccccgc tcccggcgtt tcaataactg aaaaccatac
601 tatttcacag tttaaatcac attaaacgac agtaatcccc gttgatttgt gcgccaacac
661 agatccgtcg accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca
721 cttattcagg cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac
781 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
841 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
901 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
961 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
1021 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
1081 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
1141 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
1201 tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
1261 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
1321 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
1381 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
1441 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
1501 tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag
1561 ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga
1621 tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg
1681 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
1741 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg
1801 ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt
1861 catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat
1921 attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa
1981 tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag
2041 tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga
2101 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt
2161 tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
2221 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag
2281 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat
2341 ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag
2401 ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact
2461 gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg
2521 gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa
2581 gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat
2641 ttctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga
2701 tataagttgt aattctcatg tttgacagct tatcatcgat cccgcaagag gcccggcagt
2761 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
2821 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
2881 ctaccgcatt aaagcttctt gcgaaacagc tctcgaccag gtttatactg gtcgtgcaga
2941 tgaaagcagt ttttgccgag gtcgattccg accagcgcca tatcgctcat agtgatggtc
3001 tccgaattat aaacaccctg cgaaagcgta gcctacgcag ggtgtcgggt gctcatccca
3061 ttacccgttt tcccatctct taacttaagg ggggaacggt gtcctgtctt tcatggggct
3121 cgttcactgc taacttacct gcttccctgt ctgtcaattc tctctatcct gcgatcccgc
3181 gaagaaccaa aaaagctcga cagagggcgc ggtcatttta ggtcgggcgg atcggcgccg
3241 ccggctcggc tggtgtgccg cacagcaccg cctacgtgag ctgccagttg atgaacttcc
3301 cccgttgcca gctagggcgc aagcgggctg tataagatca ctgcccatca cattgatcgg
3361 ctcggatttt ttctcaatcc gtaaacaggt caaacatcag ttgccgcaac caaatattgg
3421 ctaggtcctt gtggtacttc gcatgccaga acatgttgat ggctatttca ggcaagacga
3481 ctgggtgcgg caaggcgctt aggccgaagg gctccacaca gcagtcggct aaacgtatcg
3541 gcacagtggc gagcagatcg gtgcgctgga ggatgtggcc aacggcggcg aagtgcggca
3601 cttccagacg gatgtcgcgc cggatgccga cccgtgtcat gtacgtgtcc acctcgccgt
3661 ggccggtgcc agcggcgatg acacgcacgt ggccgtagga acagaagcgc tccagagtca
3721 ggggttcgcg ggtgactgga tggtccttgc gacataggca cacgtagtga ttctggagca
3781 gccggcgctg aaagaagcca gtttgcagat tgggaagcag gcccacggcc aagtccacgg
3841 ttccgttctg caaggcctgc atcaggctca tcgaactgtc gcgcaccgta ctgatcacgc
3901 aattgggggc ctggtgagcc agcacatcca tcagccgcgg catgaagtag atctcgccaa
3961 tgtcggtcat ggccagggtg aaggtacgct cgctggtcag cggatcgaag ctttcatggt
4021 gctgtagggc gttgcgcagt gcgtgcatgg ccgaagtgac gggctcggcc agatgcgcgg
4081 catagggtgt gggttccatt ccctgatgtg tgcgcacgaa gagtgggtcc tgtagcgagg
4141 tgcgcaggcg tttcagcgca ttgctcacgg caggctgggt caggcccagg ttctccgcag
4201 tgatagagac gcgtctgtcg accagcaact ggttgaacac caccagcagg tttaaatcca
4261 ggtcacgcag ttccatgggg cctcgcttgg gttattgctg gtgcccggcc gggcgcaata
4321 ttcatgttga tgatttatta tatatcgagt ggtgtattta tcaatattgt ttgctccgtt
4381 atcgttatta acaagtcatc aataatgcca taaggagtac catatgttta aaaaacttca
4441 catgaagatt gctgtttttg tttcgattat gttaatcatc acagttgttc tcttaatgct
4501 gtcttcttat ttgacgctaa aaccgatgat tacagaggat gggaaaaata caactcaaaa
4561 cgtgacgcag tcacttgaac aaaatattga attgcaatta aagagttatg ccatttcgtt
4621 atcaagattg gctaatgggg aattaacgca tacatttgta acgaaaccaa gtaaagaagc
4681 ttctcggctg tttcacgatg acattaagca aatcaaagac aacgatgatt atgttgcgat
4741 ggcttacata ggcaccgcaa aaaaagaaat gtttacatat ccaaaagcgg atttcgctga
4801 ggattacgat ccaacatcaa gaccatggta taagcttgca gctgaaaccc cggatcaagt
4861 tgtttggacg gaaccttata aagatgtcgt gacaggagac atgattgtta ctgcatcgaa
4921 agcgatctta gacaggcaaa aagtgatagg ggttgccagc tatgacttga aactctctgc
4981 tatccagagt atggtcaata aacaaaaggt gccttacaaa ggatttgcct ttttagctga
5041 tgcgagcggc aatcttttag cccatccgtc aaatcaagga aaaaatatat caaaggatca
5101 aacactgcaa accatcgcat ccgagaaaaa agggatacaa gatgtgaatg ggaaaatggt
5161 tgtgtaccaa acgattggcg agacaggctg gaaggtcgga acgcaatttg ataccgatca
5221 attaatgtgg atttcagata aaatgaatcg ggccaatctt tggatatcat tgattgcgct
5281 tgtggtggta ttgattctgc tggtggcgtg gtacggcatt cgccgtatgt tgcttactcc
5341 gctggcaaaa attattgctc acattcgcga aatcgccggt ggtaacctgg cgaataccct
5401 gaccattgac gggcgcagtg aaatgggcga cctggcgcag agcgtttcac acatgcaacg
5461 ctctttgact gacaccgtca ctcatgtccg cgaaggttca gatgccatct atgccggtac
5521 ccgtgaaatt gcggcgggca acaccgatct ttcctcccgt actgaacagc aggcatccgc
5581 gctggaagaa actgccgcca gcatggagca gctcaccgcg acagtgaagc aaaacgccga
5641 taacgcccgc caggcctcgc aactggcgca aagtgcctcc gacaccgccc agcacggcgg
5701 caaagtggtg gatggcgtag tgaaaacgat gcatgagatc gccgatagtt cgaagaaaat
5761 tgccgacatt atcagcgtta tcgacggtat tgccttccag actaatatcc tcgcgctgaa
5821 tgccgcggtt gaagccgcgc gtgcgggtga acagggccgt ggttttgccg tggtggcggg
5881 tgaagtgcgt aatcttgcca gtcgcagcgc ccaggcggca aaagagatca aagccctcat
5941 tgaagactcc gtctcacgcg ttgataccgg ttcggtgctg gtcgaaagcg ccggggaaac
6001 aatgaacaat atcgtcaatg ctgtcactcg cgtgactgac attatgggcg agattgcatc
6061 ggcatcggat gaacagagcc gtggcat
//
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