| 出品公司: | Clontech |
|---|---|
| 载体名称: | pRetroX-TRE3G |
| 质粒类型: | 逆病毒载体；四环素调控载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | TRE3GV |
| 载体大小: | 6600 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | DH5α |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | 逆病毒载体pRetroX-TRE3G是四环素调控系统的应答载体，需要与相应的调控载体pRetroX-Tet3G共同使用。 |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
pRetroX-TRE3G载体质粒基本信息
pRetroX-TRE3G质粒图谱载体图谱和pRetroX-TRE3G载体序列质粒序列多克隆位点信息
pRetroX-TRE3G质粒载体简介
pRetroX-TRE3G质粒序列载体序列
LOCUS pRetroX-TRE3G 6600 bp DNA SYN
DEFINITION pRetroX-TRE3G
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6600
/organism="pRetroX-TRE3G"
/mol_type="other DNA"
promoter 2..507
/label="CMV_immearly_promoter"
misc_feature 6..689
/label="5_LTR"
misc_feature 9..297
/label="CAG_enhancer"
misc_feature 464..484
/label="CMV_fwd_primer"
misc_feature 759..1568
/label="psi_plus_pack"
misc_feature 1155..1562
/label="gag"
misc_feature 1498..1520
/label="MSCV_primer"
misc_feature 1498..1520
/label="pLXSN_5_primer"
misc_feature 1536..1552
/label="pBABE_5_primer"
misc_feature 1894..1913
/label="pCEP_fwd_primer"
misc_feature 1896..1920
/label="LNCX_primer"
misc_feature complement(2041..2064)
/label="MSCV_rev_primer"
CDS 2286..3125
/label="ORF frame 3"
/translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP
KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLTMTEYKPTVRLATRDDVPRAVRT
LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE
SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG
LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK
PGA*"
misc_feature 2438..2457
/label="mPGK_F_primer"
/translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP
KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLTMTEYKPTVRLATRDDVPRAVRT
LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE
SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG
LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK
PGA*"
CDS complement(2451..3176)
/label="ORF frame 2"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
gene 2526..3125
/label="puro (variant)"
/gene="puro (variant)"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
misc_feature complement(3834..3856)
/label="pGEX_3_primer"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
misc_feature complement(3992..4012)
/label="pBABE_3_primer"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
misc_feature complement(3998..4213)
/label="SV40_enhancer"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
promoter 4010..4278
/label="SV40_promoter"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
rep_origin 4177..4254
/label="SV40_origin"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
misc_feature 4239..4258
/label="SV40pro_F_primer"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
gene complement(5378..6238)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
CDS complement(5378..6238)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(6280..6308)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 6530..507
/label="CMV_immearly_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 6594..689
/label="5_LTR"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc
241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact
541 cggcgcgcca gtcttccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct
601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg
721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact
841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
1561 taggcgccgg aattgaagat ctcgatgagg ccctttcgtc ttcactcgag tttactccct
1621 atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga acgtatgcag
1681 actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat cagtgataga
1741 gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag tttactccct
1801 atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga acgtatgtcg
1861 aggtaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc
1921 gcctggagca attccacaac acttttgtct tatacttgga tccatcgata cgcgtgcggc
1981 cgcgccggcg ggcccgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
2041 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
2101 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
2161 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
2221 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
2281 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
2341 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
2401 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
2461 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc
2521 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
2581 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
2641 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
2701 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
2761 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
2821 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
2881 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
2941 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
3001 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
3061 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
3121 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
3181 cggcgtctcg agatatcagt ggtccaggct ctagttttga ctcaacaata tcaccagctg
3241 aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct ccagaaaaag
3301 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
3361 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
3421 ggaacagggt cgaccctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
3481 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
3541 tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg
3601 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
3661 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
3721 ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc
3781 accaccggga ggtaagctgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
3841 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
3901 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
3961 cgtagcgata gcggagtgta gatccggctg tggaatgtgt gtcagttagg gtgtggaaag
4021 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
4081 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
4141 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
4201 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
4261 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
4321 tgcaaaaagc ttactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
4381 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
4441 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
4501 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
4561 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
4621 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
4681 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
4741 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
4801 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
4861 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
4921 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
4981 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
5041 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
5101 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
5161 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
5221 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
5281 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
5341 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
5401 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
5461 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
5521 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
5581 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
5641 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
5701 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
5761 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
5821 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
5881 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
5941 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
6001 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
6061 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
6121 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
6181 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
6241 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
6301 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
6361 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
6421 tcacgaggcc ctttcgtctt caagaattag cttggccatt gcatacgttg tatccatatc
6481 ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga cattgattat
6541 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
//
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pRetroX-TRE3G质粒载体应用举例
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