pHCMV-EcoEnv

LOCUS       pHCMV-EcoEnv	6763 bp 	DNA	SYN
DEFINITION  pHCMV-EcoEnv
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="pHCMV-EcoEnv"
                     /mol_type="other DNA"
     promoter        98..674
                     /label="CMV_immearly_promoter"
     misc_feature    162..988
                     /label="5_LTR"
     misc_feature    177..464
                     /label="CAG_enhancer"
     misc_feature    631..651
                     /label="CMV_fwd_primer"
     promoter        632..701
                     /label="CMV_promoter"
     promoter        644..763
                     /label="CMV2_promoter"
     misc_feature    675..694
                     /label="pCEP_fwd_primer"
     misc_feature    677..701
                     /label="LNCX_primer"
     misc_feature    792..1364
                     /label="bGlob_int"
     CDS             1455..3452
                     /label="ORF frame 3"
     terminator      3471..3913
                     /label="rb_glob_PA_terminator"
     misc_feature    complement(3958..3980)
                     /label="M13_pUC_rev_primer"
     promoter        complement(3994..4023)
                     /label="lac_promoter"
     gene            complement(5106..5967)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(5485..5967)
                     /label="ORF frame 2"
     promoter        complement(6009..6037)
                     /label="AmpR_promoter"
     misc_feature    complement(6554..6709)
                     /label="lacZ_a"
     misc_feature    6680..6702
                     /label="M13_pUC_fwd_primer"
     promoter        6695..6711
                     /label="M13_forward20_primer"
     promoter        6721..6739
                     /label="T7_promoter"
ORIGIN
    1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
   61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
  121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
  181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
  241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
  301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
  361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
  421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
  481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
  541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
  601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
  661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
  721 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg
  781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
  841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
  901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
  961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
 1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
 1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
 1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
 1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
 1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
 1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
 1381 tggttgttgt gctgtctcat cattttggca aagaattccg cgggcggccg ctctagagga
 1441 tccgctagac tgacatggcg cgttcaacgc tctcaaaacc ccttaaaaat aaggttaacc
 1501 cgcgaggccc cctaatcccc ttaattcttc tgatgctcag aggggtcagt actgcttcgc
 1561 ccggctccag tcctcatcaa gtctataata tcacctggga ggtaaccaat ggagatcggg
 1621 agacggtatg ggcaacttct ggcaaccacc ctctgtggac ctggtggcct gaccttaccc
 1681 cagatttatg tatgttagcc caccatggac catcttattg ggggctagaa tatcaatccc
 1741 ctttttcttc tcccccgggg cccccttgtt gctcaggggg cagcagccca ggctgttcca
 1801 gagactgcga agaaccttta acctccctca cccctcggtg caacactgcc tggaacagac
 1861 tcaagctaga ccagacaact cataaatcaa atgagggatt ttatgtttgc cccgggcccc
 1921 accgcccccg agaatccaag tcatgtgggg gtccagactc cttctactgt gcctattggg
 1981 gctgtgagac aaccggtaga gcttactgga agccctcctc atcatgggat ttcatcacag
 2041 taaacaacaa tctcacctct gaccaggctg tccaggtatg caaagataat aagtggtgca
 2101 accccttagt tattcggttt acagacgccg ggagacgggt tacttcctgg accacaggac
 2161 attactgggg cttacgtttg tatgtctccg gacaagatcc agggcttaca tttgggatcc
 2221 gactcagata ccaaaatcta ggaccccgcg tcccaatagg gccaaacccc gttctggcag
 2281 accaacagcc actctccaag cccaaacctg ttaagtcgcc ttcagtcacc aaaccaccca
 2341 gtgggactcc tctctcccct acccaacttc caccggcggg aacggaaaat aggctgctaa
 2401 acttagtaga cggagcctac caagccctca acctcaccag tcctgacaaa acccaagagt
 2461 gctggttgtg tctagtagcg ggacccccct actacgaagg ggttgccgtc ctgggtacct
 2521 actccaacca tacctctgct ccagccaact gctccgtggc ctcccaacac aagttgaccc
 2581 tgtccgaagt gaccggacag ggactctgca taggagcagt tcccaaaaca catcaggccc
 2641 tatgtaatac cacccagaca agcagtcgag ggtcctatta tctagttgcc cctacaggta
 2701 ccatgtgggc ttgtagtacc gggcttactc catgcatctc caccaccata ctgaacctta
 2761 ccactgatta ttgtgttctt gtcgaactct ggccaagagt cacctatcat tcccccagct
 2821 atgtttacgg cctgtttgag agatccaacc gacacaaaag agaaccggtg tcgttaaccc
 2881 tggccctatt attgggtgga ctaaccatgg ggggaattgc cgctggaata ggaacaggga
 2941 ctactgctct aatggccact cagcaattcc agcagctcca agccgcagta caggatgatc
 3001 tcagggaggt tgaaaaatca atctctaacc tagaaaagtc tctcacttcc ctgtctgaag
 3061 ttgtcctaca gaatcgaagg ggcctagact tgttatttct aaaagaagga gggctgtgtg
 3121 ctgctctaaa agaagaatgt tgcttctatg cggaccacac aggactagtg agagacagca
 3181 tggccaaatt gagagagagg cttaatcaga gacagaaact gtttgagtca actcaaggat
 3241 ggtttgaggg actgtttaac agatcccctt ggtttaccac cttgatatct accattatgg
 3301 gacccctcat tgtactccta atgattttgc tcttcggacc ctgcattctt aatcgattag
 3361 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
 3421 accagctgaa gcctatagag tacgagccat agctcgaggg cccagatctt tttccctctg
 3481 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
 3541 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
 3601 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
 3661 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
 3721 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
 3781 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
 3841 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
 3901 tcttatggag atccctcgac ggatcggccg caattcgtaa tcatgtcata gctgtttcct
 3961 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
 4021 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
 4081 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
 4141 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
 4201 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
 4261 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
 4321 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
 4381 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
 4441 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
 4501 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
 4561 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
 4621 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
 4681 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
 4741 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
 4801 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
 4861 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
 4921 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
 4981 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
 5041 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
 5101 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
 5161 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
 5221 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
 5281 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
 5341 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
 5401 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
 5461 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
 5521 aaagcgggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
 5581 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
 5641 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
 5701 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
 5761 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
 5821 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
 5881 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
 5941 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
 6001 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
 6061 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
 6121 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
 6181 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
 6241 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
 6301 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
 6361 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
 6421 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
 6481 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
 6541 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
 6601 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
 6661 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
 6721 taatacgact cactataggg cgaattggag ctccaccgcg gtg
//