| 质粒名称: | pMXs-hOCT3/4 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17217 |
| 存储实验室: | Shinya Yamanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pMXs |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4600 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | OCT3/4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1100 bp |
| 基因突变: | None |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | pMX-S1811 |
| 3' 测序引物 | |
|
文献引用方法 |
|
| 材料和方法部分: | pMXs-hOCT3/4 was a gift from Shinya Yamanaka (Addgene plasmid # 17217) |
| 参考文献部分: | Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Cell. 2007 Nov 30. 131(5):861-72. 10.1016/j.cell.2007.11.019 PubMed 18035408 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMXs-hOCT3/4 Sequencing Result 5856 bp DNA SYN
DEFINITION pMXs-hOCT3/4 Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5856
/organism="pMXs-hOCT3/4 Sequencing Result"
/mol_type="other DNA"
gene complement(674..1534)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(674..1534)
/label="ORF frame 3"
promoter complement(1576..1604)
/label="AmpR_promoter"
misc_feature 1669..2262
/label="3MoMuLV_LTR"
misc_feature 1711..2262
/label="5_LTR2"
misc_feature 2262..3543
/label="psi_plus_pack2"
misc_feature 2744..3226
/label="gag"
misc_feature 3087..3109
/label="MSCV_primer"
misc_feature 3087..3109
/label="pLXSN_5_primer"
misc_feature 3125..3141
/label="pBABE_5_primer"
misc_feature 3479..3495
/label="pBMN_5_primer"
CDS 3602..4684
/label="ORF frame 2"
misc_feature 4887..1033
/label="3MoMuLV_LTR"
misc_feature 4929..5468
/label="5_LTR2"
rep_origin complement(5763..526)
/label="pBR322_origin"
ORIGIN
1 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
61 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
121 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
181 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
241 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
301 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
361 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
421 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
481 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
541 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
601 ATCTTCACCT AGATCCTTTT GCGGCCGGCC GCAAATCAAT CTAAAGTATA TATGAGTAAA
661 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT
721 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT
781 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT
841 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT
901 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA
961 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG
1021 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT
1081 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG
1141 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG
1201 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC
1261 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA
1321 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC
1381 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT
1441 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG
1501 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
1561 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA
1621 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGCATAA TGAAAGACCC
1681 CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT GGAAAAATAC
1741 ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA GATGGAACAG CTGAATATGG
1801 GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG
1861 AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG
1921 GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG CAGTTTCTAG AGAACCATCA
1981 GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA
2041 TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC
2101 ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA GTCGCCCGGG TACCCGTGTA
2161 TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT CGCTGTTCCT TGGGAGGGTC
2221 TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA TTTGGGGGCT CGTCCGGGAT
2281 CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG AGGTAAGCTG GCCAGCAACT
2341 TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT TTATGCGCCT GCGTCGGTAC
2401 TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG GAACTGACGA GTTCGGAACA
2461 CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCGGGG GCCGTTTTTG TGGCCCGACC
2521 TGAGTCCAAA AATCCCGATC GTTTTGGACT CTTTGGTGCA CCCCCCTAAT AGGAGGGATA
2581 TGTGGTTCTG GTAGGAGACG AGAACCTAAA ACAGTTCCCG CCTCCGTCTG AATTTTTGCT
2641 TTCGGTTTGG GACCGAAGCC GCGCCGCGCG TCTTGTCTGC TGCAGCATCG TTCTGTGTTG
2701 TCTCTGTCTG ACTGTGTTTC TGTATTTGTC TGAAAATTAG GGCCAGACTG TTACCACTCC
2761 CTTAAGTTTG ACCTTAGGTC ACTGGAAAGA TGTCGAGCGG ATCGCTCACA ACCAGTCGGT
2821 AGATGTCAAG AAGAGACGTT GGGTTACCTT CTGCTCTGCA GAATGGCCAA CCTTTAACGT
2881 CGGATGGCCG CGAGACGGCA CCTTTAACCG AGACCTCATC ACCCAGGTTA AGATCAAGGT
2941 CTTTTCACCT GGCCCGCATG GACACCCAGA CCAGGTCCCC TACATCGTGA CCTGGGAAGC
3001 CTTGGCTTTT GACCCCCCTC CCTGGGTCAA GCCCTTTGTA CACCCTAAGC CTCCGCCTCC
3061 TCTTCCTCCA TCCGCCCCGT CTCTCCCCCT TGAACCTCCT CGTTCGACCC CGCCTCGATC
3121 CTCCCTTTAT CCAGCCCTCA CTCCTTCTCT AGGCGCCCCC ATATGGCCAT ATGAGATCTT
3181 ATATGGGGCA CCCCCGCCCC TTGTAAACTT CCCTGACCCT GACATGACAA GAGTTACTAA
3241 CAGCCCCTCT CTCCAAGCTC ACTTACAGGC TCTCTACTTA GTCCAGCACG AAGTCTGGAG
3301 ACCTCTGGCG GCAGCCTACC AAGAACAACT GGACCGACCG GTGGTACCTC ACCCTTACCG
3361 AGTCGGCGAC ACAGTGTGGG TCCGCCGACA CCAGACTAAG AACCTAGAAC CTCGCTGGAA
3421 AGGACCTTAC ACAGTCCTGC TGACCACCCC CACCGCCCTC AAAGTAGACG GCATCGCAGC
3481 TTGGATACAC GCCGCCCACG TGAAGGCTGC CGACCCCGGG GGTGGACCAT CCTCTAGACT
3541 GCCGGATCTA GCTAGTTAAT TAAGGATCCC AGTGTGGTGG TACGGGAATT CGCCCTTCAC
3601 CATGGCGGGA CACCTGGCTT CGGATTTCGC CTTCTCGCCC CCTCCAGGTG GTGGAGGTGA
3661 TGGGCCAGGG GGGCCGGAGC CGGGCTGGGT TGATCCTCGG ACCTGGCTAA GCTTCCAAGG
3721 CCCTCCTGGA GGGCCAGGAA TCGGGCCGGG GGTTGGGCCA GGCTCTGAGG TGTGGGGGAT
3781 TCCCCCATGC CCCCCGCCGT ATGAGTTCTG TGGGGGGATG GCGTACTGTG GGCCCCAGGT
3841 TGGAGTGGGG CTAGTGCCCC AAGGCGGCTT GGAGACCTCT CAGCCTGAGG GCGAAGCAGG
3901 AGTCGGGGTG GAGAGCAACT CCGATGGGGC CTCCCCGGAG CCCTGCACCG TCACCCCTGG
3961 TGCCGTGAAG CTGGAGAAGG AGAAGCTGGA GCAAAACCCG GAGGAGTCCC AGGACATCAA
4021 AGCTCTGCAG AAAGAACTCG AGCAATTTGC CAAGCTCCTG AAGCAGAAGA GGATCACCCT
4081 GGGATATACA CAGGCCGATG TGGGGCTCAC CCTGGGGGTT CTATTTGGGA AGGTATTCAG
4141 CCAAACGACC ATCTGCCGCT TTGAGGCTCT GCAGCTTAGC TTCAAGAACA TGTGTAAGCT
4201 GCGGCCCTTG CTGCAGAAGT GGGTGGAGGA AGCTGACAAC AATGAAAATC TTCAGGAGAT
4261 ATGCAAAGCA GAAACCCTCG TGCAGGCCCG AAAGAGAAAG CGAACCAGTA TCGAGAACCG
4321 AGTGAGAGGC AACCTGGAGA ATTTGTTCCT GCAGTGCCCG AAACCCACAC TGCAGCAGAT
4381 CAGCCACATC GCCCAGCAGC TTGGGCTCGA GAAGGATGTG GTCCGAGTGT GGTTCTGTAA
4441 CCGGCGCCAG AAGGGCAAGC GATCAAGCAG CGACTATGCA CAACGAGAGG ATTTTGAGGC
4501 TGCTGGGTCT CCTTTCTCAG GGGGACCAGT GTCCTTTCCT CTGGCCCCAG GGCCCCATTT
4561 TGGTACCCCA GGCTATGGGA GCCCTCACTT CACTGCACTG TACTCCTCGG TCCCTTTCCC
4621 TGAGGGGGAA GCCTTTCCCC CTGTCTCTGT CACCACTCTG GGCTCTCCCA TGCATTCAAA
4681 CTGAGGTAAG GGCGAATTCC AGCTGAGCGC CGGTCGCTAC CATTACCAGT TGGTCTGGTG
4741 TCAAAAATAA TAATAACCGG GCAGGCCATG TCTGCCCGTA TTTCGCGTAA GGAAATCCAT
4801 TATGTACTAT TTAAACTCGA GCGGCCGCCA GCACAGTGGT CGACGATAAA ATAAAAGATT
4861 TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA
4921 GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT AGAGAAGTTC
4981 AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA
5041 AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC
5101 AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG
5161 ATGCGGTCCA GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG
5221 GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT
5281 TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGGGCGCC
5341 AGTCCTCCGA TTGACTGAGT CGCCCGGGTA CCCGTGTATC CAATAAACCC TCTTGCAGTT
5401 GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC
5461 GTCAGCGGGG GTCTTTCACA TGCAGCATGT ATCAAAATTA ATTTGGTTTT TTTCTTAAGT
5521 ATTTACATTA AATGGCCATA GTTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
5581 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
5641 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
5701 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
5761 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
5821 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTA
//
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