pLVUT-tTR-KRAB

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pLVUT-tTR-KRAB 
出品公司: 爱迪基因
目录编号: 11651
存储实验室: Patrick Aebischer, Didier Trono

载体骨架基本信息
载体名称: None
载体抗性: 氨苄霉素
空载体大小: 11480 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: hUbiquitin C, GFP, tTR-KRAB, Tet-on
克隆方法: 限制性内切酶
5' 克隆位点:  
3' 克隆位点:  
5' 测序引物 See map
3' 测序引物  

文献引用方法
材料和方法部分: pLVUT-tTR-KRAB was a gift from Patrick Aebischer & Didier Trono (Addgene plasmid # 11651)
参考文献部分: A versatile tool for conditional gene expression and knockdown. Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P. Nat Methods. 2006 Feb . 3(2):109-16. 10.1038/nmeth846 PubMed 16432520

载体图谱质粒图谱和多克隆位点信息

pLVUT-tTR-KRAB载体图谱

载体简介

载体序列

LOCUS       pLVUT-tTR-KRAB	11512 bp 	DNA	SYN
DEFINITION  pLVUT-tTR-KRAB
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..11512
                     /organism="pLVUT-tTR-KRAB"
                     /mol_type="other DNA"
     promoter        239..815
                     /label="CMV_immearly_promoter"
     misc_feature    318..605
                     /label="CAG_enhancer"
     misc_feature    772..792
                     /label="CMV_fwd_primer"
     misc_feature    835..1015
                     /label="truncHIV-1_3_LTR"
     misc_feature    835..1015
                     /label="HIV-1_5_LTR"
     misc_feature    1126..1170
                     /label="HIV-1_psi_pack"
     CDS             1558..2445
                     /label="ORF frame 1"
     misc_feature    1680..1913
                     /label="RRE"
     misc_feature    2444..2459
                     /label="cPPT"
     promoter        2617..3833
                     /label="hUbC_promoter"
     CDS             3887..4606
                     /label="ORF frame 2"
     gene            3890..4603
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(3932..3953)
                     /label="EGFP_N_primer"
     misc_feature    4070..4099
                     /label="Y66 (EGFP)"
     misc_feature    4540..4561
                     /label="EGFP_C_primer"
     misc_feature    4641..5203
                     /label="IRES"
     CDS             5207..6214
                     /label="ORF frame 2"
     misc_feature    5222..5821
                     /label="TetR"
     misc_feature    5828..5848
                     /label="SV40 NLS"
     misc_feature    5834..5848
                     /label="NLS"
     misc_feature    6288..6875
                     /label="WPRE"
     CDS             6376..7026
                     /label="ORF frame 1"
     CDS             6389..6904
                     /label="ORF frame 2"
     misc_feature    complement(6878..6894)
                     /label="pBluescriptKS_primer"
     misc_feature    6948..6963
                     /label="cPPT"
     misc_feature    6948..6969
                     /label="U3PPT"
     misc_feature    7000..7287
                     /label="TRE"
     misc_feature    7319..7499
                     /label="HIV-1_5_LTR"
     misc_feature    7319..7499
                     /label="truncHIV-1_3_LTR"
     misc_feature    complement(7523..7540)
                     /label="BGH_rev_primer"
     terminator      7526..7753
                     /label="bGH_PA_terminator"
     rep_origin      7816..8122
                     /label="f1_origin"
     misc_feature    complement(8236..8256)
                     /label="pBABE_3_primer"
     misc_feature    complement(8242..8457)
                     /label="SV40_enhancer"
     promoter        8254..8522
                     /label="SV40_promoter"
     rep_origin      8421..8498
                     /label="SV40_origin"
     misc_feature    8483..8502
                     /label="SV40pro_F_primer"
     promoter        8616..8683
                     /label="EM7_promoter"
     gene            8684..9055
                     /label="bleo"
                     /gene="bleo"
     misc_feature    8684..9058
                     /label="sh_ble"
     terminator      9191..9310
                     /label="SV40_PA_terminator"
     misc_feature    9279..9298
                     /label="EBV_rev_primer"
     promoter        complement(9354..9372)
                     /label="M13_reverse_primer"
     misc_feature    complement(9371..9393)
                     /label="M13_pUC_rev_primer"
     promoter        complement(9407..9436)
                     /label="lac_promoter"
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(10519..11379)
                     /label="ORF frame 2"
     promoter        complement(11421..11449)
                     /label="AmpR_promoter"
ORIGIN
    1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
   61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
  121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
  181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
  241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
  301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
  361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
  421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
  481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
  541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
  601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
  661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
  721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
  781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
  841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
  901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
  961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
 2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
 2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
 2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
 2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
 2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
 2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
 2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
 3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
 3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
 3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
 3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
 3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
 3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
 3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
 3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
 3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
 3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
 3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
 3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
 3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
 3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgAAGCTT
 3841 GGGCTGCAGG TCGACTCTAG aggatccccg ggtaccggtc gccaccatgg tgagcaaggg
 3901 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
 3961 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
 4021 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
 4081 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
 4141 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
 4201 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
 4261 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
 4321 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
 4381 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
 4441 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
 4501 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
 4561 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc
 4621 tagaattcga taattccgcc cccccccccc taacgttact ggccgaagcc gcttggaata
 4681 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
 4741 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
 4801 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
 4861 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
 4921 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
 4981 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
 5041 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
 5101 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
 5161 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aataccatgg ctagattaga
 5221 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
 5281 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
 5341 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
 5401 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
 5461 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
 5521 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
 5581 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
 5641 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
 5701 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
 5761 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag
 5821 tgggtcgcca aaaaagaaga gaaaggtcga cggcggtggt gctttgtctc ctcagcactc
 5881 tgctgtcact caaggaagta tcatcaagaa caaggagggc atggatgcta agtcactaac
 5941 tgcctggtcc cggacactgg tgaccttcaa ggatgtattt gtggacttca ccagggagga
 6001 gtggaagctg ctggacactg ctcagcagat cgtgtacaga aatgtgatgc tggagaacta
 6061 taagaacctg gtttccttgg gttatcagct tactaagcca gatgtgatcc tccggttgga
 6121 gaagggagaa gagccctggc tggtggagag agaaattcac caagagaccc atcctgattc
 6181 agagactgca tttgaaatca aatcatcagt ttaaggatct cgactagcta gtagctagct
 6241 agctagtcga gctcaagctt cgaatTCGAt atcaagctta tcgataatca acctctggat
 6301 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
 6361 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
 6421 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
 6481 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
 6541 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
 6601 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
 6661 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
 6721 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
 6781 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
 6841 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagtacct
 6901 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
 6961 ggactggaag ggctaattca ctcccaacga agacaagatc tcgagtttac cactccctat
 7021 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
 7081 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
 7141 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
 7201 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
 7261 accactccct atcagtgata gagaaaagtg aaagtcggat ctgctttttg cttgtactgg
 7321 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
 7381 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
 7441 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
 7501 catctagaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
 7561 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
 7621 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
 7681 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
 7741 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
 7801 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
 7861 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
 7921 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
 7981 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
 8041 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
 8101 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
 8161 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
 8221 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
 8281 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
 8341 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
 8401 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
 8461 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
 8521 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
 8581 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
 8641 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
 8701 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
 8761 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
 8821 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
 8881 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
 8941 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
 9001 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
 9061 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
 9121 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
 9181 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
 9241 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
 9301 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
 9361 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
 9421 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
 9481 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
 9541 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
 9601 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
 9661 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
 9721 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
 9781 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
 9841 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
 9901 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
 9961 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
10021 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
10081 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
10141 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
10201 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
10261 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
10321 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
10381 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
10441 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
10501 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
10561 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
10621 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
10681 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
10741 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
10801 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
10861 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
10921 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
10981 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
11041 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
11101 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
11161 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
11221 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
11281 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
11341 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
11401 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
11461 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac

//

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