质粒名称: | pLVUT-tTR-KRAB |
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出品公司: | 爱迪基因 |
目录编号: | 11651 |
存储实验室: | Patrick Aebischer, Didier Trono |
载体骨架基本信息 |
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载体名称: | None |
载体抗性: | 氨苄霉素 |
空载体大小: | 11480 bp |
载体类型: | 哺乳细胞表达 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | hUbiquitin C, GFP, tTR-KRAB, Tet-on |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | |
3' 克隆位点: | |
5' 测序引物 | See map |
3' 测序引物 | |
文献引用方法 |
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材料和方法部分: | pLVUT-tTR-KRAB was a gift from Patrick Aebischer & Didier Trono (Addgene plasmid # 11651) |
参考文献部分: | A versatile tool for conditional gene expression and knockdown. Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P. Nat Methods. 2006 Feb . 3(2):109-16. 10.1038/nmeth846 PubMed 16432520 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVUT-tTR-KRAB 11512 bp DNA SYN DEFINITION pLVUT-tTR-KRAB ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..11512 /organism="pLVUT-tTR-KRAB" /mol_type="other DNA" promoter 239..815 /label="CMV_immearly_promoter" misc_feature 318..605 /label="CAG_enhancer" misc_feature 772..792 /label="CMV_fwd_primer" misc_feature 835..1015 /label="truncHIV-1_3_LTR" misc_feature 835..1015 /label="HIV-1_5_LTR" misc_feature 1126..1170 /label="HIV-1_psi_pack" CDS 1558..2445 /label="ORF frame 1" misc_feature 1680..1913 /label="RRE" misc_feature 2444..2459 /label="cPPT" promoter 2617..3833 /label="hUbC_promoter" CDS 3887..4606 /label="ORF frame 2" gene 3890..4603 /label="EGFP" /gene="EGFP" misc_feature complement(3932..3953) /label="EGFP_N_primer" misc_feature 4070..4099 /label="Y66 (EGFP)" misc_feature 4540..4561 /label="EGFP_C_primer" misc_feature 4641..5203 /label="IRES" CDS 5207..6214 /label="ORF frame 2" misc_feature 5222..5821 /label="TetR" misc_feature 5828..5848 /label="SV40 NLS" misc_feature 5834..5848 /label="NLS" misc_feature 6288..6875 /label="WPRE" CDS 6376..7026 /label="ORF frame 1" CDS 6389..6904 /label="ORF frame 2" misc_feature complement(6878..6894) /label="pBluescriptKS_primer" misc_feature 6948..6963 /label="cPPT" misc_feature 6948..6969 /label="U3PPT" misc_feature 7000..7287 /label="TRE" misc_feature 7319..7499 /label="HIV-1_5_LTR" misc_feature 7319..7499 /label="truncHIV-1_3_LTR" misc_feature complement(7523..7540) /label="BGH_rev_primer" terminator 7526..7753 /label="bGH_PA_terminator" rep_origin 7816..8122 /label="f1_origin" misc_feature complement(8236..8256) /label="pBABE_3_primer" misc_feature complement(8242..8457) /label="SV40_enhancer" promoter 8254..8522 /label="SV40_promoter" rep_origin 8421..8498 /label="SV40_origin" misc_feature 8483..8502 /label="SV40pro_F_primer" promoter 8616..8683 /label="EM7_promoter" gene 8684..9055 /label="bleo" /gene="bleo" misc_feature 8684..9058 /label="sh_ble" terminator 9191..9310 /label="SV40_PA_terminator" misc_feature 9279..9298 /label="EBV_rev_primer" promoter complement(9354..9372) /label="M13_reverse_primer" misc_feature complement(9371..9393) /label="M13_pUC_rev_primer" promoter complement(9407..9436) /label="lac_promoter" /label="Ampicillin" /gene="Ampicillin" CDS complement(10519..11379) /label="ORF frame 2" promoter complement(11421..11449) /label="AmpR_promoter" ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt 2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg 2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga 2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc 2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg 2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc 2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga 3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc 3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt 3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt 3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg 3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc 3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg 3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc 3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt 3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc 3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct 3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc 3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg 3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt 3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgAAGCTT 3841 GGGCTGCAGG TCGACTCTAG aggatccccg ggtaccggtc gccaccatgg tgagcaaggg 3901 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 3961 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 4021 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 4081 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 4141 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 4201 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 4261 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 4321 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 4381 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 4441 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 4501 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 4561 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc 4621 tagaattcga taattccgcc cccccccccc taacgttact ggccgaagcc gcttggaata 4681 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt 4741 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct 4801 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc 4861 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga 4921 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc 4981 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt 5041 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg 5101 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg 5161 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aataccatgg ctagattaga 5221 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt 5281 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt 5341 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac 5401 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag 5461 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc 5521 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt 5581 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt 5641 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag 5701 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc 5761 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag 5821 tgggtcgcca aaaaagaaga gaaaggtcga cggcggtggt gctttgtctc ctcagcactc 5881 tgctgtcact caaggaagta tcatcaagaa caaggagggc atggatgcta agtcactaac 5941 tgcctggtcc cggacactgg tgaccttcaa ggatgtattt gtggacttca ccagggagga 6001 gtggaagctg ctggacactg ctcagcagat cgtgtacaga aatgtgatgc tggagaacta 6061 taagaacctg gtttccttgg gttatcagct tactaagcca gatgtgatcc tccggttgga 6121 gaagggagaa gagccctggc tggtggagag agaaattcac caagagaccc atcctgattc 6181 agagactgca tttgaaatca aatcatcagt ttaaggatct cgactagcta gtagctagct 6241 agctagtcga gctcaagctt cgaatTCGAt atcaagctta tcgataatca acctctggat 6301 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 6361 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 6421 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 6481 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 6541 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 6601 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 6661 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc 6721 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 6781 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 6841 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagtacct 6901 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 6961 ggactggaag ggctaattca ctcccaacga agacaagatc tcgagtttac cactccctat 7021 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg 7081 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact 7141 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga 7201 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt 7261 accactccct atcagtgata gagaaaagtg aaagtcggat ctgctttttg cttgtactgg 7321 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 7381 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 7441 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 7501 catctagaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 7561 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 7621 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 7681 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 7741 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 7801 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 7861 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 7921 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 7981 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 8041 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 8101 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 8161 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 8221 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 8281 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 8341 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 8401 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 8461 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 8521 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 8581 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata 8641 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag 8701 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg 8761 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt 8821 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt 8881 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt 8941 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt 9001 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca 9061 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 9121 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 9181 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 9241 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 9301 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 9361 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 9421 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 9481 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 9541 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 9601 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 9661 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 9721 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 9781 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 9841 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 9901 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 9961 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 10021 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 10081 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 10141 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 10201 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 10261 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 10321 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 10381 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 10441 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 10501 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 10561 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 10621 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 10681 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 10741 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 10801 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 10861 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 10921 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 10981 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 11041 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 11101 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 11161 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 11221 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 11281 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 11341 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 11401 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 11461 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac //
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