出品人: | Bob Weinberg |
---|---|
载体名称: | pBABE-zeo |
质粒类型: | 逆病毒载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | MSCV |
载体大小: | 4888bp |
5' 测序引物及序列: | pBABE 5' primer 5'CTTTATCCAGCCCTCAC3' |
3' 测序引物及序列: | pBABE 3' primer 5'ACCCTAACTGACACACATTCC3' |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
筛选标记: | Zeocin |
克隆菌株: | DH5α、HB101等 |
宿主细胞(系): | 常规哺乳动物细胞系 |
备注: |
推荐使用逆病毒包装载体:pUMVC、pCMV-VSV-G 。 |
产品目录号: | #1766 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 逆转录病毒 |
买家导航
pBABE-zeo载体质粒基本信息
pBABE-zeo质粒图谱载体图谱和pBABE-zeo载体序列质粒序列多克隆位点信息
pBABE-zeo质粒载体简介
pBABE-zeo质粒序列载体序列
LOCUS pBABE-zeo (pBABE-bleo) 4888 bp DNA SYN DEFINITION pBABE-zeo (pBABE-bleo) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4888 /organism="pBABE-zeo (pBABE-bleo)" /mol_type="other DNA" misc_feature 12..479 /label="5_LTR2" misc_feature 18..479 /label="3MoMuLV_LTR" misc_feature 549..1350 /label="psi_plus_pack" misc_feature 1318..1334 /label="pBABE_5_primer" misc_feature complement(1408..1428) /label="pBABE_3_primer" misc_feature complement(1414..1629) /label="SV40_enhancer" promoter 1426..1694 /label="SV40_promoter" rep_origin 1593..1670 /label="SV40_origin" misc_feature 1655..1674 /label="SV40pro_F_primer" gene 1749..2120 /label="bleo" /gene="bleo" misc_feature 1749..2123 /label="sh_ble" misc_feature 2169..2762 /label="3MoMuLV_LTR" misc_feature 2211..2762 /label="5_LTR2" gene complement(3900..4760) /label="Ampicillin" /gene="Ampicillin" CDS complement(3900..4760) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(4802..4830) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt 121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt 901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt 961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc 1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg 1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca 1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct 1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt 1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt 1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga 1381 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc 1441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 1501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 1561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 1621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 1681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 1741 aaaagcttat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg 1801 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact 1861 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg 1921 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt 1981 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg 2041 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact 2101 tcgtggccga ggagcaggac tgaatcgata aaataaaaga ttttatttag tctccagaaa 2161 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt 2221 tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca 2281 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc 2341 tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc 2401 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag 2461 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt 2521 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc 2581 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga 2641 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct 2701 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca 2761 tttccgactt gtggtctcgc tgccttggga gggtctcctc tgagtgattg actacccgtc 2821 agcgggggtc ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt 2881 tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg 2941 cgtattggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 3001 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 3061 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 3121 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 3181 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 3241 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 3301 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 3361 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 3421 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 3481 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 3541 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 3601 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 3661 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 3721 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 3781 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 3841 aatgaagttt gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 3901 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 3961 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 4021 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 4081 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 4141 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 4201 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 4261 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 4321 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 4381 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 4441 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 4501 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 4561 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 4621 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 4681 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 4741 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 4801 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 4861 ccgcgcacat ttccccgaaa agtgccac //