pEF-myc-ER-GFP

价格：6500元

联系方式：I47-825O-882O

买家导航

载体基本信息

出品公司:	Invitrogen
载体名称:	pEF-myc-ER-GFP
质粒类型:	哺乳动物表达载体；亚细胞定位载体；绿色荧光报告载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	EF1
载体大小:	6299 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	cycle3 GFP绿色荧光蛋白、Myc tag （c term）
载体抗性:	氨苄青霉素
筛选标记:	Neomycin
克隆菌株:	DH5α 等
宿主细胞（系）:	哺乳动物细胞
备注:	pEF-myc-ER-GFP是亚细胞定位载体。其它细胞膜定位载体： pDsRed2-ER pECFP-ER
产品目录号:	--
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

载体图谱质粒图谱和多克隆位点信息

pEF-myc-ER-GFP载体图谱

载体简介

载体序列

GGTACCGAATTCAAGCTTCGTGAGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCG
AGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTG
ATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGA
ACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCC
TCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTCCAGTACGTGATTCTTGATCCCG
AGCTGGAGCCAGGGGCGGGCCTTGCGCTTTAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGG
GCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGC
CATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAGGA
TCTGCACACTGGTATTTCGGTTTTTGGGCCCGCGGCCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTC
GGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCT
GGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGC
GTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTCCAGGGGGCTCAAAATGGAGGACGCGGCGCTCGGGAGA
GCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCAC
GGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTGGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGG
GGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTT
GATGTAATTCTCCTTGGAATTTGGCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGT
TCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGAACACGTGGCCACCATGGGATGGAGCTGTATCATCCTCT
TCTTGGTAGCAACAGCTACAGGTAAGGGGTTAACAGTAGCAGGCTTGAGGTCTGGACATATATATGGGTGAC
AATGACATCCACTTTGCCTTTCTCTCCACAGGCGCGCACTCCCAGGTCCAACTGCAGATGGCTAGCAAAGGA
GAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCT
GTCAGTGGAGAGGGTGAAGGTGATGCTACATACGGAAAGCTTACCCTTAAATTTATTTGCACTACTGGAAAA
CTACCTGTTCCATGGCCAACACTTGTCACTACTTTCTCTTATGGTGTTCAATGCTTTTCCCGTTATCCGGAT
CATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAACGCACTATATCTTTC
AAAGATGACGGGAACTACAAGACGCGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATCGTATCGAG
TTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTCGGACACAAACTCGAGTACAACTATAACTCACAC
AATGTATACATCACGGCAGACAAACAAAAGAATGGAATCAAAGCTAACTTCAAAATTCGCCACAACATTGAA
GATGGATCCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCA
GACAACCATTACCTGTCGACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGCGTGACCACATGGTCCTT
CTTGAGTTTGTAACTGCTGCTGGGATTACACATGGCATGGATGAGCTCTACAAAGCGGCCGCAGAACAAAAA
CTCATCTCAGAAGAGGATCTGAATGGGGCCGCAAGCGAGAAGGACGAGCTGTAGTCTAGAAGCTCGCTGATC
AGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGA
AGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTC
TATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGA
TGGCCCGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTG
TAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGC
GCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCG
GGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGG
TTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAG
TGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTT
GGGGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAAT
GTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGGCAGGCAGAAGTATGCAAAGCATGCATCTCAA
TTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAA
TTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTC
TCCGCCCCTAGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCA
GAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGGTCCACAATGATTGAA
CAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAG
ACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACC
GACCTGTCCGGTGCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTT
CCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGG
CAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTG
CATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGG
ATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTC
GCCAGGCTCAAGGCGCGTATGCCCGACGGCGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCGAAT
ATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAG
GACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTT
TACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGA
CTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCT
TCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCA
TGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCA
CAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTT
ATCATGTCTGTATACCGGATCTTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTA
TATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTAT
TTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCC
CCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCG
GAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAG
CTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCAC
GCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGT
TGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCAC
TCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTG
AGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGG
ATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCT
CAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTT
TTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGA
CACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCA
TGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAG
TGCCACCTGACGTCAGATCGACGGATCGGGAGATC

p3xFLAG-KV2.1	pKO11
pIAβ8	pET302/NT-His
pMKO.1-GFP	pKLAC2
pAX01-ComK	pOS1
pSC101 and pKG2	pcDNA6.2/V5-PL-DEST
pcDNA3.1+/N-GST(TEV)	pB-gyrase_TA
pEF4/His B	pCambia1201
pSilencer5.1-H1 Retro	pFB-hrGFP
pFastBac1	pDP5rs
pME12::Tn5-259	pBApo-CMV-Pur
pGFP22	pAc5.1a
pCAMBIA3200	pET-32 EK/LIC
VaD1	DR2
pTrcHis2 C	pTT5
pFA1	pLVX-Myc-MCS-Strep-IRES-Puro
pYES2-kan	phCMV1
pCMV-3Tag-3a	pTALETF_v2 (NG)
pYES2-EGFP	pQE-32
pFastBac-GST-C	pPH3
pFtsH_TA	pDR540
pMYs-IRES-GFP	pZeoSV2 (+)
pGL4.39[luc2P/ATF6 RE/Hygro]	pIJ702-87del(78-86, 88-132)
pCTAP-Shuttle-A	pTCK303
pG5luc	pSinRep5
pcDNA4/HisMax A	pCMV5
pCR4-35	pCDF1-MCS2-EF1-Puro
pEF1/His B	pRS426
pMMB33	pDEST10
pECFP-Nuc	pGL4.25[luc2CP/minP]
PX391	pET-28a-EGFP
pNIT-3	pcDNA4/V5-His B
pGL4.77[hRlucP/Hygro]	pMV261
pVW5JI	pBT-5
pacAd5 9.2-100	pHC79-2cos/tk
IR2	pSW510
pBHR68	p53-Luc
pFN31K Nluc-CMV-neo-Flexi	pBad/Myc-His A
pTYB2	pHIC-PI
pXIS11	pUCDM
pSRE-Luc	pIB/V5-His
pLKO-DEST-EGFP	pLenti CMV/TO Neo empty (w215-1)
pET-12c(+)	pPZP122
pFastBac Dual	pMSCG19
pJW342	pWW60-1
pGL101	pCold-GST
pGL4.83[hRlucP/Puro]	pcDNA3.1/His B
pE	pLVX-rHom-Sec1
pAc5.1-V5-His A	pCas
pCas-Guide-EF1a-GFP	pCB1535
pMIB/V5-His A	pcDNA3.1+P2A-eGFP

pSELECT-HA-mFOXO1	pCI neo-hEST2
Tcf/Lef:H2B-GFP	MSP2135
pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-V	pUCmini-iCAP-AAV.CAP-B22
pRN11	pGL4-ERSE2-luc2P-Hygro
TrkC-GFP	pBabe-hygro PyMT
pDA077	pCRISPR-aBEST
pCMVInt	pcDNA3/Flag-METTL3
pF151 pcDNA3.1(+)SOD1WT	pBabe-puro-miR-10b sponge
pBS-hBAF60a	pPH37
SIRT3 Flag	pCMV-2NLS-Cas9-6XMS2
pcDNA3.1-mCherry	pYLCRISPR/Cas9pUbi-N
CaV1.2	pCMV-SynA
pMaster1	pCMVR8.74
pPBbsr2-4031NES	pCMV-ABE7.9
pCAS9-TPC	pTK299
FUGW-PercevalHR	pHyo094 (eMutaT7)
Plasmid L5 attB::Pleft* mCherry	pcDNA3.1 myc-NL-GW
pTNT-B18R-6His	MSP680
pmGFP	xCas9(3.6)-BE3
pET15b_Bst-LF	BCR/ABL P190 transgenic construct
LentiGuide Cherry	p415-GalL-Cas9-CYC1t
pGL3 2 kb prom. CD274	pMDIAI
pLentipuro3/TO/V5-GW/EGFP-Firefly Lucife	pCAG Cas9-2A-Citrine
DNMT3b KO Hyg	pEJS654 All-in-One AAV-sgRNA-hNmeCas9
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T	pCas9
pminiTol2	jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcode
Flag-TRIM24	hCas9_D10A
pEcgRNA	pY001 (pFnCpf1_full)
pMXs-hc-MYC	pRJPaph-bjGFP
pmScarlet_Giantin_C1	pCMV-HsPeg10rc3
pHIV-Luc-ZsGreen	pHAGE2-TetOminiCMV-mCherry
pRK5-p18-HA	pMSCV-FlagMLL-pl-ENL(5613)
pLenti PGK Puro DEST (w529-2)	pLV-puro-N-3HA-SREBP1-C-Flag
pHIV-iRFP720-E2A-Luc	pBECKP-km
pHAGE NFkB-TA-LUC-UBC-GFP-W	pR26 GFP Dest
BECLIN-HA WT	sfGFP-Cx43K258stop
Stat3 Flag pRc/CMV	pGM1202
pJQ200mp18	pCMVHA hEZH2
mTagBFP2-TOMM20-N-10	pET21a-BirA
pBAD18-Cm	pC0061 PsmCas13 (B05) His6-TwinStrep-SUM
mTert-pBabe-puro	pHAtC
PG13-luc (wt p53 binding sites)	pEGFP-NMHC II-C
pc DNA FRT TO- APPSwed/Ind	pHAGE2-TetOminiCMV-SKM
pRK GFP Paxillin	pGIPZ-PD-L1-EGFP
pBFC1207	pFE-sfGFP
pAAV-EF1a-DIO-mCherry	pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
pNLF1W	pAAV-EF1a-DIO-HTB
pSIN4-EF1a-LMO2-IRES-Puro	pGL3-mArg1 promoter/enhancer -31/-3810
pSBbi-GN	pNI3
cytoplasmic EKAR (Cerulean-Venus)	EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (F

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pEF-myc-ER-GFP

买家导航

载体基本信息

载体图谱质粒图谱和多克隆位点信息

载体简介

载体序列

载体图谱质粒图谱pdf版和相关资料下载

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