| 出品公司: | CAMBIA |
|---|---|
| 载体名称: | pCAMBIA3300 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 8429 bp |
| 5' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 草丁膦（磷丝菌素）phosphinothricin |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCAMBIA3300 8429 bp ds-DNA circular SYN 25-JUL-2016
DEFINITION Agrobacterium binary vector for plant transformation, with
bialophos/phosphinothricin resistance and kanamycin resistance
genes.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8429)
AUTHORS Cambia
TITLE Direct Submission
JOURNAL Exported Monday, July 25, 2016 from SnapGene Viewer 3.1.4 to Vector
NTI(R) format
http://www.snapgene.com
COMMENT VNTDATE|758505600|
COMMENT VNTDBDATE|776304000|
COMMENT VNTNAME|pCAMBIA3300|
COMMENT VNTAUTHORNAME|Cambia|
FEATURES Location/Qualifiers
primer_bind complement(60..76)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 279..303
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 1603..2232
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/label=pVS1 StaA
CDS 2661..3734
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 3800..3994
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 4338..4478
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(4664..5252)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5339..6133)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
misc_feature 6558..6582
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 6660..6834
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(6841..7392)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase草丁膦（磷丝菌素）
乙酰转移酶"
/label=BlpR
/note="confers resistance to bialophos or phosphinothricin"
promoter complement(7437..8114)
/label=35S promoter (enhanced)
/note="CaMV 35S promoter (enhanced)。cauliflower mosaic
virus 35S promoter with a duplicated enhancer region"
promoter 8341..8371
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8379..8395
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8403..8419
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 8415..219
/codon_start=1
/gene="lacZ (truncated)"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
misc_feature 8429..56
/label=MCS
/note="pUC18/19 multiple cloning site"
ORIGIN
1 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca
61 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
121 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
181 ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag
241 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
301 aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt
361 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
421 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
481 cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
541 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
601 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
661 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
721 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
781 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
841 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
901 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
961 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
1021 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
1081 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
1141 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
1201 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
1261 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
1321 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
1381 cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt
1441 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
1501 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
1561 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
1621 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
1681 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
1741 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
1801 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
1861 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
1921 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
1981 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
2041 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
2101 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
2161 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
2221 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
2281 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
2341 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
2401 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
2461 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
2521 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
2581 ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc
2641 cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg
2701 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac
2761 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca
2821 aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg
2881 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca
2941 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
3001 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca
3061 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc
3121 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg
3181 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct
3241 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
3301 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg
3361 aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca
3421 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg
3481 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat
3541 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
3601 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg
3661 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat
3721 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag
3781 gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg
3841 ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt
3901 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta
3961 ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag
4021 agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc
4081 ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag
4141 ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc
4201 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
4261 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
4321 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
4381 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
4441 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
4501 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
4561 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
4621 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
4681 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
4741 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
4801 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
4861 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
4921 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
4981 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
5041 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
5101 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
5161 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
5221 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
5281 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact
5341 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
5401 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
5461 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
5521 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
5581 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
5641 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
5701 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
5761 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
5821 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
5881 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
5941 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
6001 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
6061 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
6121 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
6181 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
6241 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
6301 gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca
6361 tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg
6421 aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg
6481 tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
6541 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
6601 cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg
6661 atctggattt tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat
6721 tttacaaata caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct
6781 ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag
6841 tcaaatctcg gtgacgggca ggaccggacg gggcggtacc ggcaggctga agtccagctg
6901 ccagaaaccc acgtcatgcc agttcccgtg cttgaagccg gccgcccgca gcatgccgcg
6961 gggggcatat ccgagcgcct cgtgcatgcg cacgctcggg tcgttgggca gcccgatgac
7021 agcgaccacg ctcttgaagc cctgtgcctc cagggacttc agcaggtggg tgtagagcgt
7081 ggagcccagt cccgtccgct ggtggcgggg ggagacgtac acggtcgact cggccgtcca
7141 gtcgtaggcg ttgcgtgcct tccaggggcc cgcgtaggcg atgccggcga cctcgccgtc
7201 cacctcggcg acgagccagg gatagcgctc ccgcagacgg acgaggtcgt ccgtccactc
7261 ctgcggttcc tgcggctcgg tacggaagtt gaccgtgctt gtctcgatgt agtggttgac
7321 gatggtgcag accgccggca tgtccgcctc ggtggcacgg cggatgtcgg ccgggcgtcg
7381 ttctgggctc atggtagact cgagagagat agatttgtag agagagactg gtgatttcag
7441 cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt
7501 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
7561 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
7621 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
7681 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
7741 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
7801 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
7861 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
7921 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
7981 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
8041 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
8101 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
8161 agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc
8221 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
8281 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
8341 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
8401 cacaggaaac agctatgacc atgattacg
//
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