| 质粒名称: | pLenti CMV/TO Puro empty (w175-1) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17482 |
| 存储实验室: | Eric Campeau |
|
载体骨架基本信息 |
|
| 载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 8007 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Bam HI(未破坏) |
| 3' 克隆位点: | Xba I(未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti CMV/TO Puro empty (w175-1) was a gift from Eric Campeau (Addgene plasmid # 17482) |
| 参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体图谱.png)
载体简介
载体序列
LOCUS pLenti CMV/TO Puro empty (w175-1) 8007 bp DNA SYN
DEFINITION pLenti CMV/TO Puro empty (w175-1)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8007
/organism="pLenti CMV/TO Puro empty (w175-1)"
/mol_type="other DNA"
promoter 62..90
/label="AmpR_promoter"
gene 132..992
/label="Ampicillin"
/gene="Ampicillin"
CDS 132..992
/label="ORF frame 3"
rep_origin 1147..1766
/label="pBR322_origin"
promoter 2075..2104
/label="lac_promoter"
misc_feature 2118..2140
/label="M13_pUC_rev_primer"
promoter 2139..2157
/label="M13_reverse_primer"
promoter 2174..2193
/label="T3_promoter"
misc_feature 2448..2628
/label="truncHIV-1_3_LTR"
misc_feature 2448..2628
/label="HIV-1_5_LTR"
misc_feature 2736..2780
/label="HIV-1_psi_pack"
CDS 3168..3668
/label="ORF frame 3"
misc_feature 3290..3523
/label="RRE"
CDS 3629..4171
/label="ORF frame 2"
misc_feature 4016..4031
/label="cPPT"
promoter 4149..4653
/label="CMV_immearly_promoter"
misc_feature 4156..4443
/label="CAG_enhancer"
misc_feature 4610..4630
/label="CMV_fwd_primer"
misc_feature 4661..4700
/label="tetO"
misc_feature 4741..4765
/label="attB1"
misc_feature 4834..4858
/label="attB2"
misc_feature 4891..5466
/label="WPRE"
CDS 4979..5608
/label="ORF frame 2"
misc_feature complement(5526..5549)
/label="MSCV_rev_primer"
/label="ORF frame 2"
misc_feature 5923..5942
/label="mPGK_F_primer"
CDS complement(5936..6661)
/label="ORF frame 3"
gene 6011..6610
/label="puro (variant)"
/gene="puro (variant)"
misc_feature 6731..6746
/label="cPPT"
misc_feature 6731..6752
/label="U3PPT"
misc_feature 6801..6981
/label="HIV-1_5_LTR"
misc_feature 6801..6981
/label="truncHIV-1_3_LTR"
terminator 7056..7175
/label="SV40_PA_terminator"
misc_feature 7144..7163
/label="EBV_rev_primer"
rep_origin 7193..7270
/label="SV40_origin"
misc_feature 7255..7274
/label="SV40pro_F_primer"
promoter complement(7361..7379)
/label="T7_promoter"
promoter complement(7389..7405)
/label="M13_forward20_primer"
misc_feature complement(7398..7420)
/label="M13_pUC_fwd_primer"
misc_feature 7398..7541
/label="lacZ_a"
rep_origin 7567..7873
/label="f1_origin"
ORIGIN
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct
4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
4321 tatCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT
4381 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC
4441 ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT
4501 GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC
4561 CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC
4621 GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC TCCCTATCAG TGATAGAGAT
4681 CTCCCTATCA GTGATAGAGA TCGTCGACTA GTCCAGTGTG GTGGAATTCT GCAGATATCA
4741 ACAAGTTTGT ACAAAAAAGC AGGCTCCACC ATGGGAACCA ATTCAGTCGA CTGGATCCGG
4801 TACCGAATTC GCGGCCGCAC TCGAGATATC TAGACCCAGC TTTCTTGTAC AAAGTGGTTG
4861 ATATCCAGCA CAGTGGCGGC CGCTCGAcaa tcaacctctg gattacaaaa tttgtgaaag
4921 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
4981 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
5041 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
5101 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
5161 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
5221 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
5281 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
5341 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
5401 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
5461 ttgggccgcc tccccgcctg GAATTCTACC GGGTAGGGGA GGCGCTTTTC CCAAGGCAGT
5521 CTGGAGCATG CGCTTTAGCA GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCTGGC
5581 CTCGCACACA TTCCACATCC ACCGGTAGGC GCCAACCGGC TCCGTTCTTT GGTGGCCCCT
5641 TCGCGCCACC TTCTACTCCT CCCCTAGTCA GGAAGTTCCC CCCCGCCCCG CAGCTCGCGT
5701 CGTGCAGGAC GTGACAAATG GAAGTAGCAC GTCTCACTAG TCTCGTGCAG ATGGACAGCA
5761 CCGCTGAGCA ATGGAAGCGG GTAGGCCTTT GGGGCAGCGG CCAATAGCAG CTTTGCTCCT
5821 TCGCTTTCTG GGCTCAGAGG CTGGGAAGGG GTGGGTCCGG GGGCGGGCTC AGGGGCGGGC
5881 TCAGGGGCGG GGCGGGCGCC CGAAGGTCCT CCGGAGGCCC GGCATTCTGC ACGCTTCAAA
5941 AGCGCACGTC TGCCGCGCTG TTCTCCTCTT CCTCATCTCC GGGCCTTTCG ACCTGCAGCC
6001 CAAGCTTACC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC
6061 CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT
6121 CGATCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT
6181 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC
6241 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA
6301 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG
6361 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA
6421 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC
6481 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC
6541 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC
6601 CGGTGCCTGA CGCCCGCCCC ACGACCCGCA GCGCCCGACC GAAAGGAGCG CACGACCCCA
6661 TGCATCTCGA GGGCCCGGTA Cctttaagac caatgactta caaggcagct gtagatctta
6721 gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag
6781 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
6841 ctggctgcct agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
6901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
6961 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
7021 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
7081 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
7141 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
7201 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
7261 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
7321 tggaggccta ggcttttgcg tcgagacgta cccaattcgc cctatagtga gtcgtattac
7381 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
7441 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
7501 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt
7561 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
7621 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
7681 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
7741 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
7801 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
7861 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
7921 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
7981 aacaaaatat taacgtttac aatttcc
//
