质粒名称: | pLenti CMV/TO Puro empty (w175-1) |
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出品公司: | 爱迪基因 |
目录编号: | 17482 |
存储实验室: | Eric Campeau |
载体骨架基本信息 |
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载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
载体抗性: | 氨苄霉素 |
空载体大小: | 8007 bp |
载体类型: | 哺乳细胞表达 |
筛选标记: | 嘌呤霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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克隆方法: | 限制性内切酶 |
5' 克隆位点: | Bam HI(未破坏) |
3' 克隆位点: | Xba I(未破坏) |
5' 测序引物 | CMV-F |
3' 测序引物 | |
文献引用方法 |
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材料和方法部分: | pLenti CMV/TO Puro empty (w175-1) was a gift from Eric Campeau (Addgene plasmid # 17482) |
参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
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pLenti CMV/TO Puro empty (w175-1)载体质粒基本信息
pLenti CMV/TO Puro empty (w175-1)质粒图谱载体图谱和pLenti CMV/TO Puro empty (w175-1)载体序列质粒序列多克隆位点信息
pLenti CMV/TO Puro empty (w175-1)质粒载体简介
pLenti CMV/TO Puro empty (w175-1)质粒序列载体序列
LOCUS pLenti CMV/TO Puro empty (w175-1) 8007 bp DNA SYN DEFINITION pLenti CMV/TO Puro empty (w175-1) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..8007 /organism="pLenti CMV/TO Puro empty (w175-1)" /mol_type="other DNA" promoter 62..90 /label="AmpR_promoter" gene 132..992 /label="Ampicillin" /gene="Ampicillin" CDS 132..992 /label="ORF frame 3" rep_origin 1147..1766 /label="pBR322_origin" promoter 2075..2104 /label="lac_promoter" misc_feature 2118..2140 /label="M13_pUC_rev_primer" promoter 2139..2157 /label="M13_reverse_primer" promoter 2174..2193 /label="T3_promoter" misc_feature 2448..2628 /label="truncHIV-1_3_LTR" misc_feature 2448..2628 /label="HIV-1_5_LTR" misc_feature 2736..2780 /label="HIV-1_psi_pack" CDS 3168..3668 /label="ORF frame 3" misc_feature 3290..3523 /label="RRE" CDS 3629..4171 /label="ORF frame 2" misc_feature 4016..4031 /label="cPPT" promoter 4149..4653 /label="CMV_immearly_promoter" misc_feature 4156..4443 /label="CAG_enhancer" misc_feature 4610..4630 /label="CMV_fwd_primer" misc_feature 4661..4700 /label="tetO" misc_feature 4741..4765 /label="attB1" misc_feature 4834..4858 /label="attB2" misc_feature 4891..5466 /label="WPRE" CDS 4979..5608 /label="ORF frame 2" misc_feature complement(5526..5549) /label="MSCV_rev_primer" /label="ORF frame 2" misc_feature 5923..5942 /label="mPGK_F_primer" CDS complement(5936..6661) /label="ORF frame 3" gene 6011..6610 /label="puro (variant)" /gene="puro (variant)" misc_feature 6731..6746 /label="cPPT" misc_feature 6731..6752 /label="U3PPT" misc_feature 6801..6981 /label="HIV-1_5_LTR" misc_feature 6801..6981 /label="truncHIV-1_3_LTR" terminator 7056..7175 /label="SV40_PA_terminator" misc_feature 7144..7163 /label="EBV_rev_primer" rep_origin 7193..7270 /label="SV40_origin" misc_feature 7255..7274 /label="SV40pro_F_primer" promoter complement(7361..7379) /label="T7_promoter" promoter complement(7389..7405) /label="M13_forward20_primer" misc_feature complement(7398..7420) /label="M13_pUC_fwd_primer" misc_feature 7398..7541 /label="lacZ_a" rep_origin 7567..7873 /label="f1_origin" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct 4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 4321 tatCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT 4381 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC 4441 ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT 4501 GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC 4561 CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC 4621 GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC TCCCTATCAG TGATAGAGAT 4681 CTCCCTATCA GTGATAGAGA TCGTCGACTA GTCCAGTGTG GTGGAATTCT GCAGATATCA 4741 ACAAGTTTGT ACAAAAAAGC AGGCTCCACC ATGGGAACCA ATTCAGTCGA CTGGATCCGG 4801 TACCGAATTC GCGGCCGCAC TCGAGATATC TAGACCCAGC TTTCTTGTAC AAAGTGGTTG 4861 ATATCCAGCA CAGTGGCGGC CGCTCGAcaa tcaacctctg gattacaaaa tttgtgaaag 4921 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 4981 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 5041 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 5101 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 5161 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 5221 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 5281 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 5341 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 5401 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct 5461 ttgggccgcc tccccgcctg GAATTCTACC GGGTAGGGGA GGCGCTTTTC CCAAGGCAGT 5521 CTGGAGCATG CGCTTTAGCA GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCTGGC 5581 CTCGCACACA TTCCACATCC ACCGGTAGGC GCCAACCGGC TCCGTTCTTT GGTGGCCCCT 5641 TCGCGCCACC TTCTACTCCT CCCCTAGTCA GGAAGTTCCC CCCCGCCCCG CAGCTCGCGT 5701 CGTGCAGGAC GTGACAAATG GAAGTAGCAC GTCTCACTAG TCTCGTGCAG ATGGACAGCA 5761 CCGCTGAGCA ATGGAAGCGG GTAGGCCTTT GGGGCAGCGG CCAATAGCAG CTTTGCTCCT 5821 TCGCTTTCTG GGCTCAGAGG CTGGGAAGGG GTGGGTCCGG GGGCGGGCTC AGGGGCGGGC 5881 TCAGGGGCGG GGCGGGCGCC CGAAGGTCCT CCGGAGGCCC GGCATTCTGC ACGCTTCAAA 5941 AGCGCACGTC TGCCGCGCTG TTCTCCTCTT CCTCATCTCC GGGCCTTTCG ACCTGCAGCC 6001 CAAGCTTACC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC 6061 CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT 6121 CGATCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT 6181 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC 6241 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA 6301 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG 6361 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA 6421 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC 6481 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC 6541 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC 6601 CGGTGCCTGA CGCCCGCCCC ACGACCCGCA GCGCCCGACC GAAAGGAGCG CACGACCCCA 6661 TGCATCTCGA GGGCCCGGTA Cctttaagac caatgactta caaggcagct gtagatctta 6721 gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag 6781 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 6841 ctggctgcct agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 6901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 6961 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 7021 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt 7081 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 7141 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc 7201 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 7261 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 7321 tggaggccta ggcttttgcg tcgagacgta cccaattcgc cctatagtga gtcgtattac 7381 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 7441 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 7501 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt 7561 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 7621 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 7681 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 7741 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 7801 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 7861 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 7921 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 7981 aacaaaatat taacgtttac aatttcc //