质粒名称: | pLenti CMV/TO Hygro empty (w214-1) |
---|---|
出品公司: | Addgene |
目录编号: | 17484 |
存储实验室: | Eric Campeau |
载体骨架基本信息 |
|
载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
载体抗性: | 氨苄霉素 |
空载体大小: | 8709 bp |
载体类型: | 哺乳细胞表达 |
筛选标记: | 潮霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
|
克隆方法: | 限制性内切酶 |
5' 克隆位点: | Bam HI(未破坏) |
3' 克隆位点: | Xba I(未破坏) |
5' 测序引物 | CMV-F |
3' 测序引物 | |
文献引用方法 |
|
材料和方法部分: | pLenti CMV/TO Hygro empty (w214-1) was a gift from Eric Campeau (Addgene plasmid # 17484) |
参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
pLenti CMV/TO Hygro empty (w214-1)载体质粒基本信息
pLenti CMV/TO Hygro empty (w214-1)质粒图谱载体图谱和pLenti CMV/TO Hygro empty (w214-1)载体序列质粒序列多克隆位点信息
pLenti CMV/TO Hygro empty (w214-1)质粒载体简介
pLenti CMV/TO Hygro empty (w214-1)质粒序列载体序列
LOCUS pLenti CMV/TO Hygro empty (w214-1) 8709 bp DNA SYN DEFINITION pLenti CMV/TO Hygro empty (w214-1) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..8709 /organism="pLenti CMV/TO Hygro empty (w214-1)" /mol_type="other DNA" promoter 62..90 /label="AmpR_promoter" gene 132..992 /label="Ampicillin" /gene="Ampicillin" CDS 132..992 /label="ORF frame 3" rep_origin 1147..1766 /label="pBR322_origin" promoter 2075..2104 /label="lac_promoter" misc_feature 2118..2140 /label="M13_pUC_rev_primer" promoter 2139..2157 /label="M13_reverse_primer" promoter 2174..2193 /label="T3_promoter" misc_feature 2448..2628 /label="HIV-1_5_LTR" misc_feature 2448..2628 /label="truncHIV-1_3_LTR" misc_feature 2736..2780 /label="HIV-1_psi_pack" CDS 3168..3668 /label="ORF frame 3" misc_feature 3290..3523 /label="RRE" CDS 3629..4171 /label="ORF frame 2" misc_feature 4016..4031 /label="cPPT" promoter 4149..4653 /label="CMV_immearly_promoter" misc_feature 4156..4443 /label="CAG_enhancer" misc_feature 4610..4630 /label="CMV_fwd_primer" misc_feature 4661..4700 /label="tetO" misc_feature 4741..4765 /label="attB1" misc_feature 4834..4858 /label="attB2" misc_feature 4891..5466 /label="WPRE" CDS 4979..5521 /label="ORF frame 2" misc_feature complement(5568..5591) /label="MSCV_rev_primer" CDS 5813..6865 /label="ORF frame 2" misc_feature 5965..5984 /label="mPGK_F_primer" CDS complement(5978..6856) /label="ORF frame 3" gene 6065..7073 /label="hygroB" /gene="hygroB" misc_feature 7433..7454 /label="U3PPT" misc_feature 7433..7448 /label="cPPT" misc_feature 7503..7683 /label="truncHIV-1_3_LTR" misc_feature 7503..7683 /label="HIV-1_5_LTR" terminator 7758..7877 /label="SV40_PA_terminator" misc_feature 7846..7865 /label="EBV_rev_primer" rep_origin 7895..7972 /label="SV40_origin" misc_feature 7957..7976 /label="SV40pro_F_primer" promoter complement(8063..8081) /label="T7_promoter" promoter complement(8091..8107) /label="M13_forward20_primer" misc_feature complement(8100..8122) /label="M13_pUC_fwd_primer" misc_feature 8100..8243 /label="lacZ_a" rep_origin 8269..8575 /label="f1_origin" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct 4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 4321 tatCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT 4381 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC 4441 ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT 4501 GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC 4561 CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC 4621 GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC TCCCTATCAG TGATAGAGAT 4681 CTCCCTATCA GTGATAGAGA TCGTCGACTA GTCCAGTGTG GTGGAATTCT GCAGATATCA 4741 ACAAGTTTGT ACAAAAAAGC AGGCTCCACC ATGGGAACCA ATTCAGTCGA CTGGATCCGG 4801 TACCGAATTC GCGGCCGCAC TCGAGATATC TAGACCCAGC TTTCTTGTAC AAAGTGGTTG 4861 ATATCCAGCA CAGTGGCGGC CGCTCGAcaa tcaacctctg gattacaaaa tttgtgaaag 4921 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 4981 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 5041 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 5101 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 5161 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 5221 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 5281 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 5341 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 5401 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct 5461 ttgggccgcc tccccgcctg gAATTCTGCA GATATCCGGA CTAGTGATCT CTCGAGGTTA 5521 ACGAATTCTA CCGGGTAGGG GAGGCGCTTT TCCCAAGGCA GTCTGGAGCA TGCGCTTTAG 5581 CAGCCCCGCT GGGCACTTGG CGCTACACAA GTGGCCTCTG GCCTCGCACA CATTCCACAT 5641 CCACCGGTAG GCGCCAACCG GCTCCGTTCT TTGGTGGCCC CTTCGCGCCA CCTTCTACTC 5701 CTCCCCTAGT CAGGAAGTTC CCCCCCGCCC CGCAGCTCGC GTCGTGCAGG ACGTGACAAA 5761 TGGAAGTAGC ACGTCTCACT AGTCTCGTGC AGATGGACAG CACCGCTGAG CAATGGAAGC 5821 GGGTAGGCCT TTGGGGCAGC GGCCAATAGC AGCTTTGCTC CTTCGCTTTC TGGGCTCAGA 5881 GGCTGGGAAG GGGTGGGTCC GGGGGCGGGC TCAGGGGCGG GCTCAGGGGC GGGGCGGGCG 5941 CCCGAAGGTC CTCCGGAGGC CCGGCATTCT GCACGCTTCA AAAGCGCACG TCTGCCGCGC 6001 TGTTCTCCTC TTCCTCATCT CCGGGCCTTT CGACCTGCAT CCCGCCACCA TGAAAAAGCC 6061 TGAACTCACC GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA 6121 CCTGATGCAG CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG 6181 TGGATATGTC CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA 6241 TCGGCACTTT GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG 6301 CGAGAGCCTG ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC 6361 TGAAACCGAA CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC 6421 GGCCGATCTT AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA 6481 CACTACATGG CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC 6541 TGTGATGGAC GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG 6601 GGCCGAGGAC TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT 6661 CCTGACGGAC AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA 6721 TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA 6781 GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC GGCTCCGGGG 6841 CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC GGCAATTTCG 6901 ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA GCCGGGACTG 6961 TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC TGTGTAGAAG 7021 TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG GAATAGAGTA 7081 GATGCCGACC GAACAAGAGC TGATTTCGAG AACGCCTCAG CCAGCAACTC GCGCGAGCCT 7141 AGCAAGGCAA ATGCGAGAGA ACGGCCTTAC GCTTGGTGGC ACAGTTCTCG TCCACAGTTC 7201 GCTAAGCTCG CTCGGCTGGG TCGCGGGAGG GCCGGTCGCA GTGATTCAGG CCCTTCTGGA 7261 TTGTGTTGGT CCCCAGGGCA CGATTGTCAT GCCCACGCAC TCGGGTGATC TGACTGATCC 7321 CGCAGATTGG AGATCGCCGC CCGTGCCTGC CGATTGGGTG CAGATCCGTC GAGGGCCCGG 7381 TACctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 7441 aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta 7501 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctgc ctagggaacc 7561 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 7621 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 7681 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 7741 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 7801 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 7861 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc 7921 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 7981 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 8041 cgtcgagacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc 8101 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 8161 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 8221 cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg 8281 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 8341 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 8401 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 8461 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 8521 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 8581 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 8641 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt 8701 acaatttcc //