质粒名称: | p18sheLL |
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出品公司: | 爱迪基因 |
目录编号: | 37321 |
存储实验室: | John Schiller |
载体骨架基本信息 |
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载体名称: | custom |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | -- |
高拷贝/低拷贝: | -- |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | HPV18L1+L2,p18LLw |
物种来源: | H. sapiens (human) |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | CMV |
标签/融合蛋白: | -- |
克隆方法: | -- |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | p18sheLL was a gift from John Schiller (Addgene plasmid # 37321 ; http://n2t.net/addgene:37321 ; RRID:Addgene_37321) |
参考文献部分: | Genital transmission of HPV in a mouse model is potentiated by nonoxynol-9 and inhibited by carrageenan. Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL, Lowy DR, Schiller JT. Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1. 10.1038/nm1598 PubMed 17603495 |
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载体基本信息
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载体序列
LOCUS p18sheLL 10723 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS p18sheLL SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10723) AUTHORS Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL, Lowy DR, Schiller JT TITLE Genital transmission of HPV in a mouse model is potentiated by nonoxynol-9 and inhibited by carrageenan. JOURNAL Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1. PUBMED 17603495 REFERENCE 2 (bases 1 to 10723) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10723 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 7..16 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" misc_feature 3659..4247 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3712..3732) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4130..4141) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(4284..4301) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4290..4514 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4560..4988 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4647..4666) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4857..4878 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4997..5017) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" CDS 6946..7314 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF ALRDPAGNCVHFVAEEQD" polyA_signal 7444..7565 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7481..7500) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7535..7554 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7614..7630) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7614..7630) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7627..7649) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7638..7654 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7662..7692) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7707..7728 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7845..7862) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8016..8601) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8096..8115) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8772..9632) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9395..9414 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9633..9737) /gene="bla" /label=AmpR promoter primer_bind complement(9812..9831) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 10003..10382 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 10383..10586 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 10537..10557 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 10588..10606 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 10609..10627 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 10637..10661 /label=LNCX /note="Human CMV promoter, forward primer" ORIGIN 1 ccggtcgcca ccatggccct ctggagacca tccgataaca cagtgtactt gcccccaccc 61 agcgtcgccc gggtggtgaa cacagacgac tacgtcacca gaacctcaat cttctaccac 121 gccgggtcca gccggctgct gaccgtgggc aacccctact tccgcgtgcc cgccggcggc 181 ggaaacaaac aagacatccc caaagtcagc gcctatcagt accgggtgtt ccgcgtccaa 241 ctgcccgatc ccaacaagtt cggcctgccc gacacctcca tctacaaccc cgagacccag 301 aggctggtct gggcttgcgc cggcgtcgag atcgggaggg gccaacccct gggcgtgggg 361 ttgtccggcc accccttcta caacaagctg gacgataccg agtccagcca cgcagcaacc 421 agcaacgtct ccgaagatgt gcgcgataac gtcagcgtgg actacaaaca aacccaactg 481 tgcatcctgg gatgcgcacc cgccatcggc gagcattggg ccaaggggac cgcctgcaag 541 agcaggcccc tgagccaagg ggactgtcca cccctggagt tgaagaatac cgtgctcgag 601 gacggcgaca tggtggacac cggctacggc gctatggatt tctccaccct ccaggacacc 661 aagtgcgaag tgcccctcga catctgccaa agcatctgca agtaccccga ctacctccag 721 atgagcgccg acccctacgg cgacagcatg ttcttctgtc tcagaaggga acaattgttc 781 gcccgccact tctggaaccg ggccggcaca atgggagata cagtccccca gagcctgtac 841 atcaagggga ccggaatgag ggccagcccc gggtcctgcg tctacagccc aagcccctcc 901 gggagcatcg tcacaagcga tagccaactc ttcaacaagc cctactggct ccacaaagcc 961 caaggccaca ataacggggt gtgttggcac aaccagctgt tcgtgaccgt cgtggacaca 1021 accaggtcca caaacctgac catctgcgcc agcacccaaa gccccgtgcc cggccagtac 1081 gacgccacaa agttcaaaca atactctcgg cacgtggaag agtacgacct ccaattcatc 1141 ttccaactct gcaccatcac cctcaccgcc gacgtgatga gctacatcca ctccatgaac 1201 tcctccatcc tggaagactg gaatttcggc gtgccaccac cccctaccac ctccctcgtc 1261 gacacctaca gattcgtgca gagcgtggcc atcacatgcc agaaagacgc cgcccccgcc 1321 gagaacaaag acccatacga caaactgaaa ttctggaacg tcgacctgaa agagaaattc 1381 agcctggatc tggaccagta cccattgggc aggaagttcc tcgtgcaagc cggcctcagg 1441 agaaaaccaa caatcgggcc caggaagagg agcgccccca gcgcaaccac cagcagcaag 1501 cccgcaaaaa gggtcagagt gagggcacgc aaatgagcta gaactagtgg atcccccggg 1561 ctgcaggaat tcgatgggtc gacctgcaga agcttaaaac agctctgggg ttgtacccac 1621 cccagaggcc cacgtggcgg ctagtactcc ggtattgcgg tacccttgta cgcctgtttt 1681 atactccctt cccgtaactt agacgcacaa aaccaagttc aatagaaggg ggtacaaacc 1741 agtaccacca cgaacaagca cttctgtttc cccggtgatg tcgtatagac tgcttgcgtg 1801 gttgaaagcg acggatccgt tatccgctta tgtacttcga gaagcccagt accacctcgg 1861 aatcttcgat gcgttgcgct cagcactcaa ccccagagtg tagcttaggc tgatgagtct 1921 ggacatccct caccggtgac ggtggtccag gctgcgttgg cggcctacct atggctaacg 1981 ccatgggacg ctagttgtga acaaggtgtg aagagcctat tgagctacat aagaatcctc 2041 cggcccctga atgcggctaa tcccaacctc ggagcaggtg gtcacaaacc agtgattggc 2101 ctgtcgtaac gcgcaagtcc gtggcggaac cgactacttt gggtgtccgt gtttcctttt 2161 attttattgt ggctgcttat ggtgacaatc acagattgtt atcataaagc gaattggatt 2221 gcggccgcat ggtcagccat agggctgcca ggaggaagag agccagcgtg accgatctgt 2281 acaagacctg caagcagagc ggcacctgcc cccccgacgt ggtgcccaaa gtcgaaggga 2341 caaccctggc cgacaagatc ctccagtgga gctctttggg catcttcctc ggcggcttgg 2401 ggatcggcac cgggtccggc accggcggca ggaccggcta tatccccctc gggggcagga 2461 gcaacaccgt cgtcgacgtg ggccccacca ggcctcccgt cgtgatcgag cccgtcgggc 2521 ctaccgatcc cagcatcgtg accctgatcg aagatagctc cgtcgtgacc agcggcgccc 2581 cccgccccac cttcaccggg accagcggct tcgacatcac cagcgccggc accaccaccc 2641 ccgccgtgct cgacattacc cccagcagca caagcgtcag catcagtacc acaaacttca 2701 caaaccccgc cttcagcgac cccagcatca tcgaggtgcc ccagaccggc gaagtcgccg 2761 gcaacgtgtt cgtgggcaca cccaccagcg gcacccacgg ctacgaagag atccccctgc 2821 agaccttcgc cagcagcggc accggcgaag agccaatctc ctccacaccc ctccccaccg 2881 tcagaagggt ggccggccca aggttgtatt cccgcgctta tcagcaagtc agcgtcgcca 2941 atcccgaatt cctgaccagg cccagcagcc tgatcaccta cgataacccc gctttcgaac 3001 ccgtcgatac caccctgacc ttcgacccca ggtccgacgt gcccgacagc gacttcatgg 3061 acatcattag gttgcaccgc cccgccctga ccagccgcag gggcaccgtg aggttctccc 3121 ggctgggcca gagagccacc atgttcacaa ggtctggcac ccagatcggc gcccgcgtgc 3181 atttctatca cgacatctcc cccatcgccc ccagccccga gtacatcgag ctccaacccc 3241 tcgtgagcgc taccgaagat aacgatctct tcgacatcta cgccgacgat atggatcccg 3301 ccgtccccgt gcccagcagg agcaccacaa gcttcgcctt cttcaagtac agcccaacca 3361 tcagcagcgc tagcagttac tccaacgtga ccgtgcccct gacaagtagc tgggacgtcc 3421 ccgtgtatac cggccccgac atcaccctgc ccagcaccac aagcgtgtgg ccaatcgtga 3481 gcccaaccgc tcccgctagc acccaataca tcggcatcca cggcacccac tactacctct 3541 ggcccctgta ctacttcatc cccaagaaga ggaagagggt gccatacttc ttcgccgacg 3601 ggttcgtcgc cgcttgagat cccccgggct gcaggaattc gatatcaagc ttatcgataa 3661 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 3721 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 3781 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 3841 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 3901 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 3961 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 4021 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 4081 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 4141 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 4201 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg 4261 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 4321 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 4381 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 4441 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 4501 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 4561 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 4621 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 4681 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 4741 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 4801 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 4861 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 4921 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 4981 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 5041 gcaggcagaa gtatgcaaag catgcagaat tctatcaaat atttaaagaa aaaaaaattg 5101 tatcaacttt ctacaatctc tttcagaaga cagaagcaga gggaatactt cctaaatcat 5161 tcaactaggc cagcattacc ttaataccgg aactagaaaa tgacattaca agaaaagaaa 5221 acaacagacc aatatctctc atgaacaaag atacaaacat tttcaacaaa atattagcaa 5281 aaagaatcca agaatgtatc aaaaaatata caccacaacc aagtagaatt tattccagat 5341 atgtaagggt ggttcaacgt ttgaaaatca attaacgtaa tttgtcccat caacaggtta 5401 aagaagaaaa tcacatggtc atattgatag acacagaaaa agcatttgac aaaatttaac 5461 acccattcat gatgcaatct ctcagtaaac taggaataga ggaaaacttc ctcagcttga 5521 atgtaccttc ctctcaattt tgctatgaac ctgaaactcc tcttaaaaaa taaagttttt 5581 catttaaaaa gaaaacaaaa aacatggagg agcgttgatg tatctcattt tagaccaatc 5641 agctatggat agttaggcga cagcacagat agctgctgta cttctgtttc tggcaatgtt 5701 ccagactaca tttaaaaaat ttttaattat agacttgtac ttaatgttca agaaaaatat 5761 gaaaatggct ttgccgtgtt aatgctactc ttttttaaaa aaaactaaag ttcaaacttt 5821 atttatattt cattagtttt ttagctactg ttctttttct gttctgggat ctcattcaga 5881 atgccacatt acatataatt ctcatgtctc cttgggttcc tcttagtttt gacagttcct 5941 cagacttttc ttatttttga tgaccttgac agttttgagg agtactggtt agatataggg 6001 taatggtttt taaagtatat ttgtcatgat ttatactggg gtaagggttt ggggaggaag 6061 cccatggggt aaagtactgt tctcatcaca tcatatcaag gttatatacc atcaatattg 6121 ccacagatgt tacttagcct tttaatattt ctctaattta gtgtatatgc aatgatagtt 6181 ctctgatttc tgagattgag tttctcatgt gtaatgatta tttagagttt ctctttcatc 6241 tgttcaaatt tttgtctagt tttatttttt actgatttgt aagacttctt tttataatct 6301 gcatattaca attctcttta ctggggtgtt gcaaatattt tctgtcattc tatggcctga 6361 cttttcttaa tggtttttta attttaaaaa taagtcttaa tattcatgca atctaattaa 6421 caatcttttc tttgtggtta ggactttgag tcataagaaa tttttctcta cactgaagtc 6481 atgatggcat gcttctatat tattttctaa aagatttaaa gttttgcctt ctccatttag 6541 acttataatt cactggaatt tttttgtgtg tatggtatga catatgggtt cccttttatt 6601 ttttacatat aaatatattt ccctgttttt ctaaaaaaga aaaagatcat cattttccca 6661 ttgtaaaatg ccatattttt ttcataggtc acttacatat atcaatgggt ctgtttctga 6721 gctctactct attttatcag cctcactgtc tatccccaca catctcatgc tttgctctaa 6781 atcttgatat ttagtggaac attctttccc attttgttct acaagaatat ttttgttatt 6841 gtcttttggg cttctatata cattttagaa tgaggttggc aagttaacaa acagcttttt 6901 tggggtgaac atattgacta caaatttatg tggaaagaaa gtaccaagtt gaccagtgcc 6961 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc 7021 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc 7081 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg 7141 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg 7201 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc 7261 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg 7321 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 7381 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 7441 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 7501 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 7561 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag 7621 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 7681 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 7741 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 7801 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 7861 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 7921 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 7981 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 8041 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 8101 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 8161 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 8221 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 8281 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 8341 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 8401 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 8461 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 8521 tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg 8581 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 8641 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 8701 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 8761 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 8821 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 8881 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 8941 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 9001 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 9061 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 9121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 9181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 9241 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 9301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 9361 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 9421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 9481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 9541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 9601 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 9661 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 9721 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga 9781 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 9841 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt 9901 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc 9961 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta 10021 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 10081 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 10141 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 10201 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 10261 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 10321 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 10381 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat 10441 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggaaccaa aatcaacggg 10501 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac 10561 ggtgggaggt ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg 10621 atagagatcg tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac 10681 gctgttttga cctccataga agacaccggg accgatccag cct //