| 质粒名称: | pMO9075 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 117481 |
| 存储实验室: | Judy Wall |
|
载体骨架基本信息 |
|
| 载体名称: | pCR8/GW/TOPO |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 2817 bp |
| 完整质粒大小: | 4836 bp |
| 载体修饰: | pCR8/GW/TOPO + pBG1 (PCR product) => pMO719 || pMO719 (EcoRV digested) + kanR (PCR product from pCR-XL-TOPO) => pMO9071 || pMO9071 (BsaBI digest, religate) => pMO9072 || pMO9072 (HpaI/XmnI digest, religate) => pMO9072-1a || pMO9072-1a (EcoRV/BspEI digest, blunted, religated) => pMO9073 || pMO9073 (EcoRI digest, religate) => pMO9075 |
| 载体类型: | 细菌表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Aph(3')-IIa promoter,Paph,Pkan |
| 物种来源: | Escherichia coli |
| 插入大小: | 50 bp |
| GenBank编号 | NC_008460.1 |
| 启动子 | Aph(3')-IIa promoter |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Blunt-end cloned（在克隆过程中被破坏） |
| 3' 克隆位点: | Blunt-end cloned（在克隆过程中被破坏） |
| 5' 测序引物 | AGACACGGGCCAGAGCTG |
| 3' 测序引物 | GCTGAAAGCGAGAAGAGCGCAC |
|
文献引用方法 |
|
| 材料和方法部分: | pMO9075 was a gift from Judy Wall (Addgene plasmid # 117481 ; http://n2t.net/addgene:117481 ; RRID:Addgene_117481) |
| 参考文献部分: | Methods for engineering sulfate reducing bacteria of the genus Desulfovibrio. Keller KL, Wall JD, Chhabra S. Methods Enzymol. 2011;497:503-17. doi: 10.1016/B978-0-12-385075-1.00022-6. 10.1016/B978-0-12-385075-1.00022-6 PubMed 21601101 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMO9075 4837 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION empty complementation vector for Desulfovibrio: contains promoter
from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons,
SpecR.
ACCESSION .
VERSION .
KEYWORDS pMO9075
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4837)
AUTHORS Keller KL, Wall JD, Chhabra S
TITLE Methods for engineering sulfate reducing bacteria of the genus
Desulfovibrio.
JOURNAL Methods Enzymol. 2011;497:503-17. doi:
10.1016/B978-0-12-385075-1.00022-6.
PUBMED 21601101
REFERENCE 2 (bases 1 to 4837)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4837
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 92..109
/label=L4440
/note="L4440 vector, forward primer"
terminator 268..295
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 387..473
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind complement(2832..2851)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2833..2851)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2856..2872)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2856..2872)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 3303..4094
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 4187..4775
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4676..4695
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taattcccct tggtggagtt gagtggtttt aaaaactgga
541 gaggagtcca tgaaacgaaa gcagctgagg attgaaacga agtaacagcg catactaatt
601 gtattttttg ggtgggggag gagccttgcg acgggcggcg agcgtagcga ggggcctcta
661 tagcgcgcgc atacgcgcgc gcgcgcgcat acgcgcgcgc ttcttctttt ctttcttttc
721 tcttttcccc ctcgtttttc gtcagttatt tcggcaacct acagacctaa aagtgaccaa
781 aagtacgtct aaaggtgacc aaaagtacgt ctaaaggtga ccaaaagtac gtctaaaggt
841 gaccaaaagt acgtggtgta gcctttgggt gaccaatttg acaagtggtc acctttagcc
901 ataaaggcaa aagaacagcc cgaaaaggtg cggtaacacc tcaacgggcc taacctctca
961 caagccctaa aatggagggt ctgcaatggt tgacaggcaa ttaaccttac ccggcgagat
1021 agtcaaaaag tcaaacgcac tggctagggc caagtggtcc cctaagtccg tgtgggaacc
1081 gcgcatggtc gcgctggtag cttccatggt ccgtcaggac gatgcggact ttcaggaata
1141 ccgctttcct gtctccaccc tttgcgatga gcaagagcta tccggcaaga ggtacaagga
1201 cattaaggcc gctgtagaaa atgtgatgaa agcctatgtg atgattgagg aaggcaaaaa
1261 ttacgtggcg tatagcatct tctcaaagtg tggctatgaa aacggtgtca tggtcgccgc
1321 cttccatccc gatctgaaac cccattttct cggccttcaa aagcagttca caaggtatag
1381 cctcatggaa tttctggtgc tgcctagcac ctacagccaa agggttttcg aggttttgag
1441 gtcgtgggac gacaagccgg aagtgaccat ttcacttgtc gatcttcatg aaatggtggg
1501 tacgcctcct tctctgcgga agaagtaccc cgactttcgc cgtaaggttc ttgagaaggc
1561 ccacaaggac ataaaccgcc ataccgcgct tgcttacgag tgggaagccg tgaagaacgg
1621 gcgctcggtc gcgggtatcc gcttcctgtt cgcgcagaag cgggcgggca gggtcgtagc
1681 caagaaacag gccaaggtcg tgcaggacag cagcgacgcc aagaacaagg ccttcatcgc
1741 cgcgaccaag tgccacgcgg ccagcaaggg ccgctgcacg ccgaaagaca acctccagtg
1801 ccaagtgtgc gcccagctcg tcagcctggg caagagagcc ggaacagcct aaacctcttt
1861 tgtagcaccc cccctttgag gctgtagcac cccccttttg aggctgtagc accttgtgac
1921 gtgcctgtag caccttttca gacttcacat ttttaaccat aacgtgctgt tttaacagaa
1981 attttcttta cgtttcagca ccggctgatc tcgctttttt gtgccacaaa aaggctatcc
2041 tgccaagcgg cattaaaaca agccacctca tcaacaaaat gaaggggggc gcggcccgct
2101 cacatggttg caggtgaaga agattggaaa tcgaggcgaa ggttattcca ttccccgcag
2161 tagaacctga acagggaagc ctgttctaat agccctaaaa tcgattttaa gcgcaggaac
2221 aagcaagaag agactcaggc tcaccgaggg aaatttcctc ccttacgccc ccttctacgc
2281 ttcgttatcg ctacgccgaa aaaaagagca agggacgctt cgcttctgcc cttgctcttt
2341 ttttgcgcct tcgctgcact ccgctcacga aggcggctcc gggggaaatt tcagcctggt
2401 tccaacgaca agcgaagcgg gatctgcggg gggcccccgt gagggggggc ggctagcccc
2461 atcccctgct tgcaggggca ggaccggcgt agccggagcg agtggtgatg gtcttgtgat
2521 cgtccagagt gatttccgtt gtgctggact ctcccgattc tcgacgagct gaaagcgaga
2581 agagcgcact tatacatcgc tgcgcgacgt gtgcgccctg cggggctaag tcatcggaac
2641 aaaagagttt ccccgtagtg gggaaaaggg ggcttgaatt cgcccttgca tgcgtttaaa
2701 ctacgtaagg agtactgcgc agatcgtgat cccctgcgcc atcagatcct tggcggcaag
2761 aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc
2821 aattccggat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
2881 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
2941 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
3001 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
3061 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
3121 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
3181 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
3241 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
3301 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
3361 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
3421 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
3481 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
3541 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
3601 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
3661 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
3721 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
3781 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
3841 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
3901 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
3961 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
4021 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
4081 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
4141 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
4201 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
4261 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
4321 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
4381 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
4441 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
4501 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
4561 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
4621 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
4681 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
4741 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
4801 ggttcctggc cttttgctgg ccttttgctc acatgtt
//
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