质粒名称: | PSD95-TS:YFP |
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出品公司: | Addgene |
目录编号: | 42225 |
存储实验室: | Roger Tsien |
载体骨架基本信息 |
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载体名称: | pCAGGS |
载体出品公司: | -- |
空载体大小: | -- |
完整质粒大小: | 9947 bp |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | -- |
载体抗性: | 氨苄霉素,100 μg/mL |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 高拷贝 |
插入基因信息 |
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插入基因名称: | PSD95 |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子: | -- |
标签/融合蛋白: | TS:YFP(插入件上的C端子) |
克隆方法: | -- |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | -- |
3’测序引物 | -- |
文献引用方法 |
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材料和方法部分: | PSD95-TS:YFP was a gift from Roger Tsien (Addgene plasmid # 42225 ; http://n2t.net/addgene:42225 ; RRID:Addgene_42225) |
参考文献部分: | Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy. Butko MT, Yang J, Geng Y, Kim HJ, Jeon NL, Shu X, Mackey MR, Ellisman MH, Tsien RY, Lin MZ. Nat Neurosci. 2012 Nov;15(12):1742-51. doi: 10.1038/nn.3246. Epub 2012 Oct 28. 10.1038/nn.3246 PubMed 23103964 |
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载体基本信息
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载体序列
LOCUS PSD95-TS:YFP 9947 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS PSD95-TS:YFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9947) AUTHORS Butko MT, Yang J, Geng Y, Kim HJ, Jeon NL, Shu X, Mackey MR, Ellisman MH, Tsien RY, Lin MZ TITLE Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy. JOURNAL Nat Neurosci. 2012 Nov;15(12):1742-51. doi: 10.1038/nn.3246. Epub 2012 Oct 28. PUBMED 23103964 REFERENCE 2 (bases 1 to 9947) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9947 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 4..383 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 386..661 /label=chicken beta-actin promoter intron 662..1670 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1594..1616 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" CDS 3936..4400 /codon_start=1 /product="improved N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VN155(I152L) /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA" primer_bind complement(3981..4002) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4242..4261) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" CDS 5259..5285 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind 5483..5504 /label=EGFP-C /note="EGFP, forward primer" CDS 5547..5573 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(6971..6990) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 7036..7091 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(7090..7109) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(7450..7466) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7450..7466) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7463..7485) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7474..7490 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7498..7528) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7543..7564 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7623..7818 /label=SV40 promoter /note="SV40 early promoter" rep_origin 7669..7804 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7731..7750 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 7824..7958 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7861..7880) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7915..7934 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8026..8043) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8197..8785) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8277..8296) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8956..9816) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9579..9598 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9817..9921) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg 541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc 1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg 1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attccggact ctagcctagg 1741 ccgcggccag gccccccaac atggactgtc tctgtatagt gacaaccaag aaataccgct 1801 accaagatga agacacgccc cctctggaac acagcccggc ccacctcccc aaccaggcca 1861 attctccccc tgtgattgtc aacacggaca ccctagaagc cccaggatat gtgaacggaa 1921 cagaggggga gatggagtat gaggagatca cattggaaag gggtaactca ggtctgggct 1981 tcagcatcgc aggtggcacc gacaacccac acatcggtga cgacccatcc atctttatca 2041 ccaagatcat tcctggtggg gctgcagccc aggatggccg cctcagggtc aacgacagca 2101 tcctgtttgt caatgaagtg gatgtccggg aggtgaccca ttcagctgca gtggaggccc 2161 tcaaagaggc gggttccatc gttcgcctct acgtcatgcg ccggaaaccc ccagctgaga 2221 agatcataga gatcaagctt atcaaagggc ctaaaggact tggcttcagc atcgcagggg 2281 gcgttgggaa ccagcacatc cctggagata atagcatcta cgtaaccaag atcatcgaag 2341 gaggcgctgc ccacaaggat ggcaggttgc agatcggaga caagatcctg gcggtcaaca 2401 gtgtggggct agaggatgtc atgcatgagg acgccgtggc agccctgaag aacacatatg 2461 acgttgtgta cctaaaggtg gccaagccca gcaatgccta cctgagtgac agctatgctc 2521 ccccagacat cacaacctca tattctcagc acctggacaa tgagatcagt catagcagct 2581 acttgggcac cgactacccc acagccatga cccccacttc ccctcggcgc tactcccccg 2641 tggccaagga cctgctaggg gaggaagata ttccccggga accaaggcgg atcgtgatcc 2701 atcggggctc caccggcctg ggcttcaaca ttgtgggcgg cgaggacggt gaaggcatct 2761 tcatctcctt catccttgct gggggtccag ctgacctcag tggggagcta cggaaggggg 2821 accagatcct gtcggtcaat ggtgttgacc tccgcaatgc cagtcatgaa caggctgcca 2881 ttgccctgaa gaacgcgggt cagacggtca cgatcatcgc tcagtataaa ccagaagagt 2941 atagccgatt cgaggccaag atccatgatc ttcgggaaca gcttatgaat agtagcctgg 3001 gctcagggac tgcatctctg cgaagcaacc ccaagcgggg cttctatatc agggccctgt 3061 ttgactacga caagaccaag gactgcggtt tcttgagcca ggccctgagc ttccactttg 3121 gggatgtgct tcatgtaatt gacgccagcg acgaagagtg gtggcaagcg cggcgggtcc 3181 actctgacag tgagaccgat gacattggct tcattcccag caaacggcgg gtcgagcgac 3241 gagagtggtc aaggttaaag gccaaggact ggggctccag ctctggatca cagggtcgag 3301 aagactcggt tctgagctat gagacggtga cgcagatgga agtgcactac gctcgcccca 3361 tcatcatcct tgggcctacc aaagaccgtg ccaacgatga tcttctctcc gagttccccg 3421 acaagtttgg atcctgtgtc cctcatacga cacgtcctaa gcgggaatat gagatagacg 3481 gccgcgatta ccactttgtc tcctcccggg agaaaatgga gaaggacatt caggcgcaca 3541 agttcattga ggctggccag tacaacagcc acctctacgg gaccagcgtc cagtctgtgc 3601 gagaggtagc agagcagggg aagcactgca tccttgatgt ctcagccaat gccgtgcggc 3661 ggctgcaggc ggcccacctg caccctatcg ccatcttcat ccgtccccgc tccctggaga 3721 atgtgctaga gatcaataag cggatcacag aggagcaagc ccggaaagcc ttcgacaggg 3781 ccacgaagct ggagcaggag ttcacggagt gcttctcagc catcgtagag ggcgacagct 3841 ttgaagagat ctatcacaaa gtgaaacgtg tcatcgaaga cctctcaggc ccctacatct 3901 gggtcccagc ccgagagaga ctggagcccg ggcagatggt gagcaagggc gaggagctgt 3961 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 4021 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct 4081 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc 4141 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 4201 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 4261 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 4321 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 4381 acaacgtcta tatcaccgcc gacaagcagt tcgaaaccgg gcccatggcc agcatgactg 4441 gtggacagca aatggggtcg acggaggacg tggtgtgctg ccactcaatc tacggcaaga 4501 agaagggtga tatcgacacc taccgataca taggctcttc cgggacaggc tgcgtggtca 4561 tagtgggcag gatcgtcttg tccggatccg gcactagtgc gcccatcacg gcgtacgccc 4621 agcagacgag aggcctccta gggtgtataa tcaccagcct gactggccgg gacaaaaacc 4681 aagtggaggg tgaggtccag atcgtgtcaa ctgctaccca aaccttcctg gcaacgtgca 4741 tcaatggggt atgctgggca gtctaccacg gggccggaac gaggaccatc gcatcaccca 4801 agggtcctgt catccagatg tataccaatg tggaccaaga ccttgtgggc tggcccgctc 4861 ctcaaggttc ccgctcattg acaccctgta cctgcggctc ctcggacctt tacctggtca 4921 cgaggcacgc cgatgtcatt cccgtgcgcc ggcgaggtga tagcaggggt agcctgcttt 4981 cgccccggcc catttcctac ttgaaaggct cctcgggggg tccgctgttg tgccccgcgg 5041 gacacgccgt gggcctattc agggccgcgg tgtgcacccg tggagtggct aaagcggtgg 5101 actttatccc tgtggagaac ctagagacaa ccatgagatc cccggtgttc acggacaact 5161 cctctccacc agcagtcacc ctgacgcacc caatcaccaa aatcgatagg gaggttctct 5221 accaggagtt cgatgagatg gaagagtgct ctcagcacta tccctacgat gtgcccgatt 5281 acgctggcgc gtctgcatgc cagaagaacg gcatcaaggc caacttcaag atccgccaca 5341 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 5401 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaag 5461 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 5521 ctctcggcat ggacgagctg tacaagtatc cctacgatgt gcccgattac gctggcgcgc 5581 cttgattcct gccctggctt ggcctggact caccctgcct ccaccacctg ggcccttggt 5641 ctggactgaa tagcccaagc ccttcgtacc ctagcctttc ttcccaccct tcttatttat 5701 ttcctttctg actggatcga gcccattgga gggaggacac tcctctctgc atgtatccct 5761 gcaccccaga tcagggcccc cagaccccag gaacctgggg tctggggggg agctgggctc 5821 ctggttccga gcccttgctc cttaagactc ctacccctgc ccgcccccat gcacacacag 5881 acccactggg ggcctctgcc ctccccattt tctcccacac acattccaga agtcagggcc 5941 cctctcaagg agtacccgct gtagggatgc agggccacag gcttccgcgc tctcccgagg 6001 caggggctgg ggtcacccct gccccatcat aactccccac gccgtttgag ttctccttta 6061 ttttctccat tcttttcttc tcaaaggtgg tttttggggg gagaagcagg ggggctctcc 6121 tgagggtccc cccgtctttc acacacctcc cacctttttt ctttgccggt ttgcatgagt 6181 ggaaggtcta actgtggctt tttttttttt ctgggatttt atttcatttt atttgatttt 6241 atttttttgg gaaaagggga gggatgggtc tggggagtgg ggaatgtggg aggggggtgg 6301 gggggcaaag ggtcaggggt tgggtgtccg ggagccaggg gaggacagga aatgctgccg 6361 ccttctgcaa tttatttatt tttttctttt gagagagtga aaggaagaga cagacacttg 6421 aaaccccgtg tgtggcctgg ttctttgggg tgagggaggg aagtggcctg ggatgggtgg 6481 ctctgagaac ttcagatcct tccttacctc acagagtata gggtgcctaa aggggtcatg 6541 ggaaggactt accagaccga ggcagacaga gctgccaccg ccgggcctgg gaagagccgg 6601 agctgggtta gcccctggca gactcgccat tgatccctgc aagctcagtc gagccctgac 6661 cactccccct agctggcctc atccctggtg acagcacatt catccctaaa gagaaataca 6721 gaggctcctc ggcttacagt ggagtgaaga ggacctatgt ataaaactac ccaggatgta 6781 caaattcaat acacctaacc ggccaaacat cagggctcgg ctccacccat ccttaaaggc 6841 tcagcacaca ggcccgcggt tgggcaaagc ctgttgtgtc acaaagcact gaacagctcg 6901 tgtgacggcc tgggagctga cgctactgct gctcagcatc aagtgccacc caatgcggcc 6961 gctcgacgga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc 7021 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt 7081 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt 7141 atttggttta gagtttggca acatatgcca tatgctggct gccatgaaca aaggtggcta 7201 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa 7261 gccttgactt gaggttagat ttttttttat attttgtttg tgttattttt ttctttaaca 7321 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc 7381 ccagtcatag ctgtccctct tctcttatga agatccctcg acctgcagcc caagcttggc 7441 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 7501 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 7561 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat 7621 ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 7681 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 7741 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 7801 ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa 7861 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 7921 tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca 7981 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 8041 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 8101 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 8161 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 8221 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 8281 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 8341 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg 8401 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 8461 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 8521 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 8581 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 8641 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 8701 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 8761 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 8821 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 8881 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 8941 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 9001 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 9061 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 9121 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 9181 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 9241 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 9301 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 9361 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 9421 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 9481 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 9541 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 9601 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 9661 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 9721 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 9781 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 9841 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 9901 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctg //
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