| 质粒名称: | pJJB308 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 107699 |
| 存储实验室: | Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pMOD_B |
| 载体抗性: | 氨苄青霉素 |
| 载体类型: | 植物表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 5' 测序引物 | GGAAGAGCGCCCACCTGC |
| 3' 测序引物 | CAAATAGGGGTTCATCGCAC |
|
文献引用方法 |
|
| 材料和方法部分: | pJJB308 was a gift from Daniel Voytas (Addgene plasmid # 107699 ; http://n2t.net/addgene:107699 ; RRID:Addgene_107699) |
| 参考文献部分: | RNA targeting with CRISPR-Cas13. Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F. Nature. 2017 Oct 4. doi: 10.1038/nature24049. 10.1038/nature24049 PubMed 28976959 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJJB308 2611 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION AtU6 Promoter-LwaCas13a-gRNA Scaffold.
ACCESSION .
VERSION .
KEYWORDS pJJB308
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2611)
AUTHORS Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J,
Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES,
Voytas DF, Ting AY, Zhang F
TITLE RNA targeting with CRISPR-Cas13.
JOURNAL Nature. 2017 Oct 4. doi: 10.1038/nature24049.
PUBMED 28976959
REFERENCE 2 (bases 1 to 2611)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2611
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 23..611
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 512..531
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 765..782
/label=L4440
/note="L4440 vector, forward primer"
misc_RNA 1321..1395
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 1475..1566
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctactaactc
121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgctcac
361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
781 aggaagcgga agagcgccca cctgccaggg gacccatggc ttcgttgaac aacggaaact
841 cgacttgcct tccgcacaat acatcatttc ttcttagctt tttttcttct tcttcgttca
901 tacagttttt ttttgtttat cagcttacat tttcttgaac cgtagctttc gttttcttct
961 ttttaacttt ccattcggag tttttgtatc ttgtttcata gtttgtccca ggattagaat
1021 gattaggcat cgaaccttca agaatttgat tgaataaaac atcttcattc ttaagatatg
1081 aagataatct tcaaaaggcc cctgggaatc tgaaagaaga gaagcaggcc catttatatg
1141 ggaaagaaca atagtatttc ttatataggc ccatttaagt tgaaaacaat cttcaaaagt
1201 cccacatcgc ttagataaga aaacgaagct gagtttatat acagctagag tcgaagtagt
1261 gattgattta gactacccca aaaacgaagg ggactaaaac gggtcttcga gaagaccttt
1321 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
1381 gcaccgagtc ggtgcttttt ttcccggtga cgcaggtgat gaaaagcctt agggtcaggt
1441 ggcacttttc ggggaaatgt gcgatgaacc cctatttgtt tatttttcta aatacattca
1501 aatatgtatc cgctaatgag acaataaccc tgataaatgc ttcaattata ttgaaaaagg
1561 aagagtatga gtatccaaca tttccgtgtc gcccttattc ccttttttgc ggccttctgc
1621 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
1681 ggtgcccgag tgggttacat tgaactggat ctcaacagcg gcaaaatctt agagagtttt
1741 cgccccgaag aacgcttccc aatgatgagc actttcaaag ttctgctatg tggcgcggtg
1801 ttatcccgta ttgatgctgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
1861 gacttggttg agtattcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
1921 gaattatgta gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
1981 acgattggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
2041 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
2101 acgatgcctg tagcaatggc aacaactctc cgcaaactat taactggcga actacttact
2161 cttgcttcac gccagcaact cattgactgg atggaggcgg acaaagttgc aggaccactt
2221 ctgcgctcgg cacttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
2281 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
2341 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
2401 ggtgcctcac tgattaagca ttggtaactg tcagacgaag ggaaataaat agtagcccgc
2461 ctgatgtgcg ggcttttttt tggtggtggt taccagtgtc gcttagctac cgtaggtcct
2521 cttaggcggt ccgattagcg acatagtcat caatgccttg acggcaaaat cccttaacgt
2581 gagttttcgt tccactgagc gtcagacccc g
//
