| 质粒名称: | pHel3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 102961 |
| 存储实验室: | Rainer Haas |
|
载体骨架基本信息 |
|
| 载体名称: | pDH10 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 穿梭质粒载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHel3 was a gift from Rainer Haas (Addgene plasmid # 102961 ; http://n2t.net/addgene:102961 ; RRID:Addgene_102961) |
| 参考文献部分: | A stable shuttle vector system for efficient genetic complementation of Helicobacter pylori strains by transformation and conjugation. Heuermann D, Haas R. Mol Gen Genet. 1998 Mar;257(5):519-28. PubMed 9563837 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHel3 5507 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION E.coli/H.Pylori shuttle vector.
ACCESSION .
VERSION .
KEYWORDS pHel3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5507)
AUTHORS Heuermann D, Haas R
TITLE A stable shuttle vector system for efficient genetic complementation
of Helicobacter pylori strains by transformation and conjugation.
JOURNAL Mol Gen Genet. 1998 Mar;257(5):519-28.
PUBMED 9563837
REFERENCE 2 (bases 1 to 5507)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5507
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(2304..3098)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin complement(3868..4456)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3948..3967)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
oriT 4682..4791
/note="incP origin of transfer"
ORIGIN
1 catttttgat agtagtgatt ttagtggtaa tcttgacaca gccactatat taaaacctta
61 gcgttttaat aacccttata agtccgccaa gacttcttaa gggtttcact cctgttatta
121 tatcgtcttt tgaaaaataa gcattaaaag gcgcttaaat gcccatgaat acgaattttg
181 aacagcttag aaaacaagaa ttggaattac gaaaattatt agaagaatta gaaacgctcc
241 cacaaacccc acaaattaaa ctgcaaaaac aaaaaataca aacttacata gacaagataa
301 caccaagtat tttgagcggt tttgatcaaa aattcaaaga aattatagaa aatctatcaa
361 atgaatttga aaaagaaaaa tccacaccac tcaaagagcc acaaaccacc cccacaccat
421 gcaaagattt agtggttagc acccctaaag ataacaccta taccacctac cacaataacg
481 ctaataaggt caatctaggg aaattgagcg aaagggaagc caatctttta ttcgctattt
541 ttcaaaaact caaagatcaa gggaataccc tcattcgttt tgaaccgcaa gatttgaaac
601 gcatgatcat agtcaaatcc aacctaacca acagacaatt attgcaaatc ctcaaaaact
661 tacttgacaa cataggcggt gctaattttt ggatcattag agagcatgtt gaaaatggcg
721 aaatctatga agatcacact agctacatgc ttttcaaaca atttgacatt cgtatccata
781 agccaaccca aactatagaa tacttagaag tccaactcaa cgatagctac cactacctgc
841 tcaacaatct aggcatgagc ggtcaataca cttcctttaa acttttagaa ttccaaaggg
901 tgaggggcaa atacgccaag acgctctatc gcttactcaa gcaatacaaa agcacaggga
961 ttttgagcgt ggaatgggat caattcaggg agcttttaga cattccaaaa gattacaaaa
1021 tggaaaacat cgatcaaaaa gtcttaactc caagcctcaa ggaactcaga aaaatctatc
1081 cctttgaaca cttgagctac aaaaaagaac gcaaaagcca cgacaaacgc aaggtaacgc
1141 atattgattt ttattttgag caattgccac agggcgaaac caagaaacaa aaacaagccg
1201 acaagcaacg cgctcaaagg gacatcaagc tcatagcgtg ggatattcac aaccaaatcg
1261 ctaaaagaaa cgctaaagcc actatagaag ctaggtttct tgagctaaaa accttgattg
1321 gttatcagtt caggcacaac aatgggacta ttttacagat tgacaacgcc acttttgaaa
1381 agaatcaaat gtttttgcat gtttcaacca gcaaaaattc tcaaaaattc cttgtgtcca
1441 acaaaacatt cgctttagaa cttctgtttg tcaatggata cagcctaaaa aaagacatgt
1501 tggaaattga tcccccaagt atccacccca tcaccaacga acctatcaag gaatttgcag
1561 aatacatcgg aaaaacgatc cacatcacca atttcaatgt ggatcaatgc cctgagggga
1621 tcaataacta cctgaaaatc actaggattg tgaaactgaa tgacaatcgg atctgtgttt
1681 cagtccaaga tgtggataag cctgagaaac ttctaaaacc tttcattgct aaagatgaaa
1741 aacatttgaa aaattggttt aagaaacact atcggtgaaa aataagtggt gggattaaca
1801 atcgtccccc acaccttgca atgtagttag ttttgcatta ttttttctat aatctctatc
1861 aattctgaga gaacatagat taattcaatg aaatttttca ttatctattt cctttctgta
1921 aattttgtta aatatttatt aaaaaaaaat ttaaagatca ctctttaagt ggaacataca
1981 aaggctgtag agagtgccac attaagcctg atgttttgct tgtgtataga gtgaaaggca
2041 atattttaac tttagttagg cttggcagtc atagtgaatt gttttagaca aatttctttc
2101 taaaatcgcc cagaatgaat ttttaattct aatttaatat aattcatcgg ttttcactca
2161 aaacgatgaa atagggctat ttttagctca ttattggggt attttgtttt tagttgaaag
2221 ctaacttcaa aattcaaaaa aacttcattt ccctttctag aggatcgatc cccggggatc
2281 ttttagacat ctaaatctag gtactaaaac aattcatcca gtaaaatata atattttatt
2341 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
2401 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
2461 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
2521 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
2581 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
2641 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
2701 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
2761 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
2821 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
2881 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
2941 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccaac
3001 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
3061 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc
3121 tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt
3181 tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt
3241 tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatga tagaatttac
3301 aagctataag gttattgtcc tgggtttcaa gcattagtcc atgcaagttt ttatgctttg
3361 cccattctat agatatattg ataagcgcgc tgcctatgcc ttgccccctg aaatccttac
3421 atacggcgat atcttctata taagcgtacc ggttccaatt ttttcgcagt ttaacttttc
3481 cgacgcattt atcgtcttgg tagcttttta aatatggcgc ttcatagagt aattctgtaa
3541 aggtccaatt ctcgttttca tacctcggta taatcttacc tatcacctca aatggttcgc
3601 tgggtttgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc aggcatgcaa
3661 gctagctttc gcgagctcga gatctagata tcgatgaatt caaaaaaaaa ggctccaaaa
3721 ggagccttta atgatctaat tcgtatcagc tcactcaaag gcggtaatac ggttatccac
3781 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
3841 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
3901 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
3961 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
4021 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
4081 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
4141 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
4201 cttatcgcca ccggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
4261 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
4321 tatctgcgct ctgctgaaac cagttacctt cggaaaaaga gttggtagct cttgatccgg
4381 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
4441 aaaaaaagga tctcaagaag atcctttgat ctctgcagtt cagtaatttc ctgcatttgc
4501 ctgtttccag tcggtagata ttccacaaaa cagcagggaa gcagcgcttt tccgctgcat
4561 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat
4621 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc
4681 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt
4741 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg
4801 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gctgcagaag cttgtccgtt
4861 agcaaatata atgtgtttag cacctaaaag cgcatctatc ttgtgttagc catctaacct
4921 aaacaaaacc gcctagcgag cgttagcgag catggacaaa agcgcatcgc agtttgaaat
4981 cgtaggcgtt agccggagat ggttttgcgt tagcaaaatc aaacaagata gcgcaaaccg
5041 aggcgtcagc caaaaaaacc ctaaaactaa aatccccaaa atatgtagcg cgtcatgcgc
5101 gttgtttata ttacatgttt taataactat gttgttttta catgttttta caacgcgcgc
5161 gcgtgtgagg gattgggggt tgcaaccccc taaataacga agctgtaggg tttctcattt
5221 ttgggttaaa atgaataaaa cagaacttct tacgagtgat aacagaactt cttacgagtg
5281 ataacagaac ttcttacgag tgataacaga acttcttacg agtgataaca gaacttcttt
5341 attgtgaagt tatgattatt aacaattttt agctaaaata acaacctaag gcggtgtaac
5401 atggttgcta atcctagtgt taacaaattt ggagcaatta gctttaaaag ctagtgggtt
5461 gggagtttgt agcgggtagc actccgttag gaggcacacc atgaaag
//
