| 载体名称: | pRU663 |
|---|---|
| 来源历史: | <- R. Schmitt |
| 质粒抗性: |
Ap抗性, Cm抗性, incP, Tc抗性, tra+, trfAts
|
| 载体说明: | 转座子Tn1725（8.9kb）在体内插入ts结合的广宿主范围R质粒pME305（Apr，Tcr）（pME305：：Tn1725）；Tn1725本身是Tn1722的Cmr变体，通过将含有cat基因的R质粒Sa的3.3kb HindIII片段连接到Tn1722的单个HindIII位点获得；可用于各种革兰氏阴性菌的诱变。 |
| 宿主菌： | Escherichia coli K12 JM108 |
| 培养条件： | LB培养基，30度 |
| 参考文献： |
Heffron, F., (1983). Tn3 and its relatives. In Mobile Genetic Elements , pp. 223-260. Edited by J. A. Shapiro, Academic Press. Rella, M., Mercenier, A., Haas, D. (1985). Transposon insertion mutagenesis of Pseudomonas aeruginosa with a Tn5 derivative: application to physical mapping of the arc gene cluster. Gene 33 : 293-303 . Schmitt, R., Altenbuchner, J., Wiebauer, K., Arnold, W., Pühler, A., Schöffl, F. (1981). Basis of transposition and gene amplification by Tn1721 and related tetracycline resistance transposons. Cold Spring Harb.Symp.Quant.Biol. 45 : 59-65 . Ubben, D., Schmitt, R. (1986). Tn1721 derivatives for transposon mutagenesis, restriction mapping and nucleotide sequence analysis. Gene 41 : 145-152 . |
| 安全等级： | 1级 |
| 分离国家: | 原产国未知 |
| 存储时间: | 1990年3月16日前 |
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