| 载体名称: | pRU664 |
|---|---|
| 来源历史: | <- R. Schmitt |
| 质粒抗性: |
Ap抗性, incP, Km抗性, Tc抗性, tra+, trfAts
|
| 载体说明: | 将转座子Tn1732（6.7kb）插入ts结合的广宿主范围R质粒pME305（Apr，Tcr）（pME305：：Tn1732）中，Tn1732本身是Tn1722的Kmr变异体，将位于1.45kb MluI-PstI片段上的Tn2680的kan基因替换为Tn1722的0.4kb MluI-PstI片段；可用于各种革兰氏阴性菌的诱变。 |
| 宿主菌： | Escherichia coli K12 DH1 |
| 培养条件： | LB培养基，30度 |
| 参考文献： |
Heffron, F., (1983). Tn3 and its relatives. In Mobile Genetic Elements , pp. 223-260. Edited by J. A. Shapiro, Academic Press. Iida, S., Meyer, J., Linder, P., Goto, N., Nakaya, R., Reif, H. J., Arber, W. (1982). The kanamycin resistance transposon Tn2680 derived from the R plasmid Rts1 and carried by phage P1Km has flanking 0,8 kb long direct repeats. Plasmid 8 : 187-198 . Mollet, B., Clerget, M., Meyer, J., Iida, S. (1985). Organization of the Tn6-related kanamycin resistance transposon Tn2680 carrying two copies of IS26 and an IS903 variant, IS903.B. J.Bacteriol. 163 : 55-60 . Rella, M., Mercenier, A., Haas, D. (1985). Transposon insertion mutagenesis of Pseudomonas aeruginosa with a Tn5 derivative: application to physical mapping of the arc gene cluster. Gene 33 : 293-303 . Schmitt, R., Altenbuchner, J., Wiebauer, K., Arnold, W., Pühler, A., Schöffl, F. (1981). Basis of transposition and gene amplification by Tn1721 and related tetracycline resistance transposons. Cold Spring Harb.Symp.Quant.Biol. 45 : 59-65 . Ubben, D., Schmitt, R. (1986). Tn1721 derivatives for transposon mutagenesis, restriction mapping and nucleotide sequence analysis. Gene 41 : 145-152 . |
| 安全等级： | 1级 |
| 分离国家: | 原产国未知 |
| 存储时间: | 1990年3月16日前 |
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