| 质粒名称: | pCMV/SB11 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26552 |
| 存储实验室: | Perry Hackett |
|
载体骨架基本信息 |
|
| 载体名称: | pCMVbeta |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3712 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Sleeping Beauty,SB,SB11 |
| 物种来源: | -- |
| 插入大小: | 1020 bp |
| 基因突变: | Methionine 243 to Glutamine Threonine 136 to Arginine Valine 253 to Histidine Alanine 255 to Arginine |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NotI（克隆过程中销毁） |
| 3' 克隆位点: | NotI（克隆过程中销毁） |
| 5' 测序引物 | M13 Forward |
| 3' 测序引物 | M13 Reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV/SB11 was a gift from Perry Hackett (Addgene plasmid # 26552 ; http://n2t.net/addgene:26552 ; RRID:Addgene_26552) |
| 参考文献部分: | Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Ivics Z, Hackett PB, Plasterk RH, Izsvak Z. Cell. 1997 Nov 14. 91(4):501-10. 10.1016/S0092-8674(00)80436-5 PubMed 9390559 |
买家导航
pCMVSB11载体基本信息
pCMVSB11载体图谱质粒图谱和多克隆位点信息
pCMVSB11载体简介
pCMVSB11载体序列
LOCUS pCMV/SB11 4732 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCMV/SB11
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4732)
AUTHORS Ivics Z, Hackett PB, Plasterk RH, Izsvak Z
TITLE Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon
from fish, and its transposition in human cells.
JOURNAL Cell. 1997 Nov 14. 91(4):501-10.
PUBMED 9390559
REFERENCE 2 (bases 1 to 4732)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4732
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 76..98
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 90..107
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 91..107
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
enhancer 134..437
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 438..641
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 592..612
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 638..662
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 779..875
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
polyA_signal 1978..2112
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2002..2021)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2056..2075
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2223..2239)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2223..2239)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(2236..2258)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 2247..2263
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2271..2301)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2316..2337
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(2454..2471)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2625..3213)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2705..2724)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3384..4244)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4007..4026
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4245..4349)
/gene="bla"
/label=AmpR promoter
primer_bind 4417..4435
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(4473..4495)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 4595..4614
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
61 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgagcttg
121 catgcctgca ggtcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
181 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
241 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
301 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
361 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
421 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
481 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
541 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
601 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
721 gcctccggac tctagaggat ccggtactcg aggaactgaa aaaccagaaa gttaactggt
781 aagtttagtc tttttgtctt ttatttcagg tcccggatcc ggtggtggtg caaatcaaag
841 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
901 tctaaaagct gcggaattgt acccgcggcc gatccgacat catgggaaaa tcaaaagaaa
961 tcagccaaga cctcagaaaa aaaattgtag acctccacaa gtctggttca tccttgggag
1021 caatttccaa acgcctgaaa gtaccacgtt catctgtaca aacaatagta cgcaagtata
1081 aacaccatgg gaccacgcag ccgtcatacc gctcaggaag gagacgcgtt ctgtctccta
1141 gagatgaacg tactttggtg cgaaaagtgc aaatcaatcc cagaacaaca gcaaaggacc
1201 ttgtgaagat gctggaggaa acaggtacaa aagtatctat atccacagta aaacgagtcc
1261 tatatcgaca taacctgaaa ggccgctcag caaggaagaa gccactgctc caaaaccgac
1321 ataagaaagc cagactacgg tttgcaagag cacatgggga caaagatcgt actttttgga
1381 gaaatgtcct ctggtctgat gaaacaaaaa tagaactgtt tggccataat gaccatcgtt
1441 atgtttggag gaagaagggg gaggcttgca agccgaagaa caccatccca accgtgaagc
1501 acgggggtgg cagcatcatg ttgtgggggt gctttgctgc aggagggact ggtgcacttc
1561 acaaaataga tggcatcatg aggaaggaaa attatgtgga tatattgaag caacatctca
1621 agacatcagt caggaagtta aagcttggtc gcaaatgggt cttccaacaa gacaatgacc
1681 ccaagcatac ttccaaacac gtgagaaaat ggcttaagga caacaaagtc aaggtattgg
1741 agtggccatc acaaagccct gacctcaatc ctatagaaaa tttgtgggca gaactgaaaa
1801 agcgtgtgcg agcaaggagg cctacaaacc tgactcagtt acaccagctc tgtcaggagg
1861 aatgggccaa aattcaccca acttattgtg ggaagcttgt ggaaggctac ccgaaacgtt
1921 tgacccaagt taaacaattt aaaggcaatg ctaccaaata ctagaattgg ccgcggggat
1981 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
2041 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
2101 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
2161 tgggaggttt tttcggatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca
2221 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
2281 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
2341 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
2401 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
2461 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
2521 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
2581 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
2641 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
2701 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
2761 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
2821 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
2881 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
2941 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
3001 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
3061 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
3121 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
3181 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
3241 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
3301 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
3361 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
3421 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
3481 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
3541 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
3601 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
3661 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
3721 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
3781 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
3841 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
3901 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
3961 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
4021 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
4081 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
4141 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
4201 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
4261 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
4321 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
4381 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
4441 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
4501 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
4561 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
4621 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
4681 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg gg
//
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