pQP-iRIS

LOCUS       pQP-iRIS                9403 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Regulation of protein localization 
            (near-infrared-blue-light-inducible shuttle).
ACCESSION   .
VERSION     .
KEYWORDS    pQP-iRIS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Redchuk TA, Omelina ES, Chernov KG, Verkhusha VV
  TITLE     Near-infrared optogenetic pair for protein regulation and spectral 
            multiplexing.
  JOURNAL   Nat Chem Biol. 2017 Mar 27. doi: 10.1038/nchembio.2343.
  PUBMED    28346403
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9403
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      616..625
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2859..3569
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLER
                     VTAAGITLGMDELYK"
     primer_bind     complement(2898..2919)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3159..3178)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    3624..3680
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3682..4256
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3706..4258
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3850..3867)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4077..4096
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4315..5022
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4460..4478)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4728..4747
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5527..5541
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     polyA_signal    6191..6312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6228..6247)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6282..6301
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6319..6774)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6456..6477)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6668..6687
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6801..6905
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6907..7264
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(6930..6950)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7115..7250
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7177..7196
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7299..8093
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7353..7372)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7963..7982
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8281..8300)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8325..8372
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8701..9289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9190..9209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctaccatg gtggcaggtc atgcctctgg cagccccgca ttcgggaccg
      661 ccgatctttc gaattgcgaa cgtgaagaga tccacctcgc cggctcgatc cagccgcatg
      721 gcgcgcttct ggtcgtcagc gagccggatc atcgcatcat ccaggccagc gccaacgccg
      781 cggaatttct gaatctcgga agcgtgctcg gcgttccgct cgccgagatc gacggcgatc
      841 tgttgatcaa gatcctgccg catctcgatc ccaccgccga aggcatgccg gtcgcggtgc
      901 gctgccggat cggcaatccc tccacggagt acgacggtct gatgcatcgg cctccggaag
      961 gcgggctgat catcgagctc gaacgtgccg gcccgccgat cgatctgtcc ggcacgctgg
     1021 cgccggcgct ggagcggatc cgcacggcgg gctcgctgcg cgcgctgtgc gatgacaccg
     1081 cgctgctgtt tcagcagtgc accggctacg accgggtgat ggtgtatcgc ttcgacgagc
     1141 agggccacgg cgaagtgttc tccgagcgcc acgtgcccgg gctcgaatcc tatttcggca
     1201 accgctatcc gtcgtcggac attccgcaga tggcgcggcg gctgtacgag cggcagcgcg
     1261 tccgcgtgct ggtcgacgtc agctatcagc cggtgccgct ggagccgcgg ctgtcgccgc
     1321 tgaccgggcg cgatctcgac atgtcgggct gcttcctgcg ctcgatgtcg ccgatccatc
     1381 tgcagtacct gaagaacatg ggcgtgcgcg ccaccctggt ggtgtcgctg gtggtcggcg
     1441 gcaagctgtg gggcctggtt gcctgtcacc attatctgcc gcgcttcatc catttcgagc
     1501 tgcgggcgat ctgcgaactg ctcgccgaag cgatcgcgac gcggatcacc gcgcttgaga
     1561 gcttcgcgca gagccagtcg gagctgttcg tgcagcggct cgaacagcgc atgatcgaag
     1621 cgatcacccg tgaaggcgat tggcgcgcag cgattttcga caccagccaa tcgatcctgc
     1681 agccgctgca cgccgacggt tgcgcgctgg tgtacgaaga ccagatcagg accatcggtg
     1741 acgtaccttc cacgcaggat gttcgcgaga tcgccgggtg gctcgatcgc cagccacgtg
     1801 cggcggtgac ctcgaccgcg tcgctcggtc tcgacgtgcc ggagctcgcg catctgacgc
     1861 ggatggcgag cggcgtggtc gcggcgccga tttcggatca tcgcggcgag tttctgatgt
     1921 ggttccgccc cgagcgcgtc cacaccgtta cctggggcgg cgatccgaag aagccgttca
     1981 cgatgggcga tacaccggcg gatctgtcgc cgcggcgctc cttcgccaaa tggcatcagg
     2041 ttgtcgaagg cacgtccgat ccgtggacgg ccgccgatct cgccgcggct cgcaccatcg
     2101 gtcagaccgt cgccgacatc gtgctgcaat tccgcgcggt gcggacactg atcgcccgcg
     2161 aacagtacga acagttttcg tcccaggtgc acgcttcgat gcagccggtg ctgatcaccg
     2221 acgccgaagg ccgcatcctg ctgatgaacg actcgttccg cgacatgttg ccggcggggt
     2281 cgccatccgc cgtccatctc gacgatctcg ccgggttctt cgtcgaatcg aacgatttcc
     2341 tgcgcaacgt cgccgaactg atcgatcacg gccgcgggtg gcgcggcgaa gttctgctgc
     2401 gcggcgcagg taatcgcccg ttgccgctgg cagtgcgcgc cgatccggtg acgcgcacgg
     2461 aggaccagtc gctcggcttc gtgctgatct tcagcgacgc taccgatcgt cgcaccgcag
     2521 atgccgcacg cacgcgtttc caggaaggca ttcttgccag cgcacgtccc ggcgtgcggc
     2581 tcgactccaa gtccgacctc ttgcacgaga agctgctgtc cgcgctggtc gagaacgcgc
     2641 agcttgccgc attggaaatt acttacggcg tcgagaccgg acgcatcgcc gagctgctcg
     2701 aaggcgttcg ccagtcgatg ctgcgcaccg ccgaagtgct cggccatctg gtgcagcacg
     2761 cggcgcgcac ggccggcagc gacagctcga gcaatggctc gcagaacaag aaggctagca
     2821 gtgctggcgg tagtgctggt agtgctggtg gcactagtag caagggcgag gagctgttca
     2881 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     2941 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca
     3001 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc
     3061 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3121 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3181 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3241 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3301 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     3361 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3421 gcgacggccc cgtgctgctg cccgacaacc actacctaag ctaccagtcc aagctgagca
     3481 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga acgcgtgacc gccgccggga
     3541 tcactctcgg catggacgag ctgtacaagg ctagcggtaa aaagaagaaa aagaagtcaa
     3601 agacaaagtg tgtaattatg taaagatctc gagctcaagc ttcgaattct gcagtcgacg
     3661 gtaccgcggg cccgggatcc gcccctctcc ctcccccccc ccctaacgtt actggccgaa
     3721 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     3781 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     3841 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     3901 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     3961 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     4021 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     4081 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     4141 gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     4201 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     4261 gccacaaacc ggtccatggg aattgatctc tcaggattaa cactgcagac tagtatggtg
     4321 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     4381 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     4441 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     4501 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     4561 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     4621 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     4681 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     4741 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     4801 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     4861 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     4921 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     4981 gagggccgcc actccaccgg cggcatggac gagctgtaca agagtgctgg tgggtccgcc
     5041 ggcgggagtg ctggcggtag tgccggcggt agttctagag gcaagaacat gcaggcggtc
     5101 accgagctgc attcccggct gatcgctgcg cagcaggcga tggagcgcga ctattggcgg
     5161 ttgcgtgaat tggagactcg ctaccgcctg gtgttcgacg ctgccgccga tgcggtgatg
     5221 atcgtctccg ccggcgacat gcgcatcgtc gaagccaacc gggcggcggt gaatgcgatc
     5281 agccgcgtcg agcgcggcaa tgacgacctt gcggggcgtg atttcctcgc cgaagtggcg
     5341 gctgccgatc gcgatgcggt gcgcgacatg ctggcccagg tgcgtcagcg cggcaccgca
     5401 ctcagcgtcc tcgttcatct cggccgttac gaccgcgcct ggatgctgcg cggttcgctg
     5461 atgtcgtccg agcgtcgtca ggttttcctg ctgcacttca ccccggtgac cacgactccc
     5521 gcgatcgacg acgacgacaa aggtgtcgtt gcttctgctg ccgacggtgc agagggcgcc
     5581 tcagacgacg cagaggatga attcccggct agcggcgccg gcgccttttt ggctactaca
     5641 cttgaacgta ttgagaagaa ctttgtcatt actgacccaa ggttgccaga taatcccatt
     5701 atattcgcgt ccgatagttt cttgcagttg acagaatata gccgtgaaga aattttggga
     5761 agaaactgca ggtttctaca aggtcctgaa actgatcgcg cgacagtgag aaaaattaga
     5821 gatgccatag ataaccaaac agaggtcact gttcagctga ttaattatac aaagagtggt
     5881 aaaaagttct ggaacctctt tcacttgcag cctatgcgag atcagaaggg agatgtccag
     5941 tactttattg gggttcagtt ggatggaact gagcatgtcc gagatgctgc cgagagagag
     6001 ggcgttatgc tggccaagaa gaccgccgag aacatcgaca tggctgccaa gagggtcaag
     6061 ttggactgag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     6121 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     6181 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6241 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6301 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     6361 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     6421 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     6481 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     6541 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     6601 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     6661 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     6721 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     6781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6901 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6961 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7021 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7081 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7141 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7201 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7261 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     7321 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     7381 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     7441 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     7501 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     7561 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     7621 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     7681 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     7741 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     7801 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     7861 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     7921 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     7981 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     8041 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     8101 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     8161 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8221 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     8281 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     8341 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     8401 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     8461 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     8521 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     8581 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8641 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8701 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8761 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8821 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8881 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8941 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9001 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9061 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9121 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9181 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9241 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9301 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9361 gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//