| 质粒名称: | pCiPS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 60011 |
| 存储实验室: | David Russell |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV beta |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7164 bp |
| 完整质粒大小: | 7842 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Pol |
| 物种来源: | human spumaretrovirus (foamy virus) |
| 插入大小: | 4152 bp |
| 基因突变: | -- |
| GenBank编号 | U21247.1 |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NotI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCiPS was a gift from David Russell (Addgene plasmid # 60011 ; http://n2t.net/addgene:60011 ; RRID:Addgene_60011) |
| 参考文献部分: | Improved foamy virus vectors with minimal viral sequences. Trobridge G, Josephson N, Vassilopoulos G, Mac J, Russell DW. Mol Ther. 2002 Sep;6(3):321-8. S1525001602906726 [pii] PubMed 12231167 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCiPS 7846 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Foamy virus helper plasmid expressing Pol, driven by a CMV promoter.
Ampicillin resistant..
ACCESSION .
VERSION .
KEYWORDS pCiPS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7846)
AUTHORS Trobridge G, Josephson N, Vassilopoulos G, Mac J, Russell DW
TITLE Improved foamy virus vectors with minimal viral sequences.
JOURNAL Mol Ther. 2002 Sep;6(3):321-8.
PUBMED 12231167
REFERENCE 2 (bases 1 to 7846)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7846
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 27..330
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 331..534
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 485..505
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 531..555
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 672..768
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
polyA_signal 4985..5119
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5009..5028)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 5063..5082
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(5230..5246)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5230..5246)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5243..5265)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5254..5270
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5278..5308)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5323..5344
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5461..5478)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5632..6220)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5712..5731)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6391..7251)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7014..7033
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7252..7356)
/gene="bla"
/label=AmpR promoter
primer_bind 7424..7442
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(7480..7502)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 7602..7621
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7815..7837
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 7829..7846
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 7830..7846
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gaattcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
601 cgggaccgat ccagcctccg gactctagag gatccggtac tcgaggaact gaaaaaccag
661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg gccgcaaaaa tcaaggagga
841 tataatcttc gaccccgtac ttaccaacct caaaggtacg gaggaggacg tggacgaaga
901 tggaacgata atactaacaa tcaagagtcc agaccatcag atcaaggttc tcaaactcct
961 aggccaaatc aagcaggctc tggggtgcgt ggcaatcagt cacaaactcc cagaccagct
1021 gctggtcgcg gaggaagagg taaccacaac cgaaaccaac gatcatccgg tgctggtgac
1081 tcacgcgctg tcaataccgt gacacagagt gccacgtcct ccacagatga atcctcttca
1141 gctgttacag ccgcttccgg cggagatcaa agggactaaa ttgttagccc actgggattc
1201 aggggcaaca ataacttgta ttcctgaaag ttttttagaa gatgaacaac ctattaaaaa
1261 gactttaata aaaacaattc atggagaaaa acaacaaaat gtttattatg taacctttaa
1321 agttaaagga agaaaagtgg aagcagaagt gatagcttct ccttatgagt atattttgct
1381 gtcgccaaca gatgttcctt ggttaacaca gcaaccactt cagttaacaa ttttagttcc
1441 tcttcaagaa tatcaagaga aaatcttaag taagactgct cttccagaag atcaaaaaca
1501 acaattaaaa accttgtttg tcaagtatga caatctatgg caacattggg aaaatcaagt
1561 cgggcataga aaaattaggc cacataatat agcaactggt gattatcctc ctcgccctca
1621 aaaacaatat cctattaatc ctaaggcaaa gcctagtata caaattgtaa tagatgactt
1681 attgaaacaa ggggtgttaa cgcctcaaaa tagtacaatg aatacaccag tgtatcctgt
1741 tcctaaacca gatggaaggt ggagaatggt attagattat agagaagtaa ataaaactat
1801 tccattaaca gctgcccaaa accaacactc tgctggtatt ttagctacta ttgttagaca
1861 aaaatataaa actaccttag atttagctaa tggattttgg gctcatccta ttacaccaga
1921 atcttattgg ttaacagcat ttacctggca aggtaaacag tattgttgga cacgtcttcc
1981 tcaaggattt ttaaatagtc cagcattgtt tacagctgat gtagtagatt tactaaaaga
2041 aatccctaat gtacaagtgt atgttgatga tatatattta agccatgatg atcctaaaga
2101 gcatgttcaa caattagaaa aagtgtttca aattttacta caggcaggat atgtagtatc
2161 tttgaaaaaa tcagaaattg gtcaaaaaac tgtagaattt ttaggattta atattactaa
2221 agaaggtcgt ggcctaacag acacttttaa aacaaaactg ttaaatatta ctcctccaaa
2281 agacttaaag caattacaaa gcatattagg attgttaaat tttgctagaa attttatacc
2341 taattttgct gaactggtac aaccattata caatttaata gcctcagcaa aaggcaaata
2401 tattgagtgg tctgaagaaa atactaaaca attaaatatg gtaatagaag cattaaacac
2461 tgcctctaat ttagaagaaa ggttaccaga acagagactg gtaattaaag tcaatacttc
2521 tccatcagca ggatatgtaa gatattataa tgagactggt aaaaagccta ttatgtacct
2581 aaattatgtg ttttccaaag cagaattaaa attttctatg ttagaaaaac tattaactac
2641 aatgcacaaa gccttaatta aggctatgga tttggccatg ggacaagaaa tattagttta
2701 tagtcccatt gtatctatga ctaaaataca aaaaactcca ctaccagaaa gaaaagcttt
2761 acccattaga tggataacat ggatgactta tttagaagat ccaagaatcc aatttcatta
2821 tgataaaacc ttaccagaac ttaagcatat tccagatgta tatacatcta gtcagtctcc
2881 tgttaaacat ccttctcaat atgaaggagt gttttatact gatggctcgg ccatcaaaag
2941 tcctgatcct acaaaaagca ataatgctgg catgggaata gtacatgcca catacaaacc
3001 tgaatatcaa gttttgaatc aatggtcaat accactaggt aatcatactg ctcagatggc
3061 tgaaatagct gcagttgaat ttgcctgtaa aaaagcttta aaaatacctg gtcctgtatt
3121 agttataact gatagtttct atgtagcaga aagtgctaat aaagaattac catactggaa
3181 atctaatggg tttgttaata ataagaaaaa gcctcttaaa catatctcca aatggaagtc
3241 tattgctgag tgtttatcta tgaaaccaga cattactatt caacatgaaa aagggcatca
3301 gcctacaaat accagtattc atactgaagg caatgcccta gcagataagc ttgccaccca
3361 aggaagttat gtagttaatt gtaataccaa aaaaccaaac ctggatgcag agttggatca
3421 attattacag ggtcattata taaaaggata tcccaaacaa tatacatatt ttttagaaga
3481 tggcaaagta aaagtttcca gacctgaagg ggttaaaatt attccccctc agtcagacag
3541 acaaaaaatt gtgcttcaag cccacaattt ggctcacacc ggacgtgaag ccactctttt
3601 aaaaattgcc aacctttatt ggtggccaaa tatgagaaag gatgtggtta aacaactagg
3661 acgctgtcaa cagtgtttaa tcacaaatgc ttccaacaaa gcctctggtc ctattctaag
3721 accagatagg cctcaaaaac cttttgataa attctttatt gactatattg gacctttgcc
3781 accttcacag ggatacctat atgtattagt agttgttgat ggaatgacag gattcacttg
3841 gttatacccc actaaggctc cttctactag cgcaactgtt aaatctctca atgtactcac
3901 tagtattgca attccaaagg tgattcactc tgatcaaggt gcagcattca cttcttcaac
3961 ctttgctgaa tgggcaaagg aaagaggtat acatttggaa ttcagtactc cttatcaccc
4021 ccaaagtagt ggtaaggtgg aaaggaaaaa tagtgatata aaacgacttt taactaaact
4081 gctagtagga agacccacaa agtggtatga cctattgcct gttgtacaac ttgctttaaa
4141 caacacctat agccctgtat taaaatatac tccacatcaa ctcttatttg gtatagattc
4201 aaatactcca tttgcaaatc aagatacact tgacttgacc agagaagaag aactttctct
4261 tttacaggaa attcgtactt ctttatacca tccatccacc cctccaacct cctctcgttc
4321 ctggtctcct gttgttggcc aattggtcca ggagagggtg gctaggcctg cttctttgag
4381 acctcgttgg cataaaccgt ctactgtact taaggtgttg aatccaagga ctgttgttat
4441 tttggaccat cttggcaaca acagaactgt aagtatagat aatttaaaac ctacttctca
4501 tcagaatggc accaccaatg acactgcaac aatggatcat ttggaacaaa atgaataaag
4561 cgcatgaggc acttcagaat acaacaactg tgactgaaca gcagaaggaa caaattatac
4621 tggacattca aaatgaagaa gtacaaccaa ctaggagaga taaatttaga tatctgcttt
4681 atacttgttg tgctactagc tcaagagtat tggcctggat atttttagtt tgtatattgt
4741 taatcattgt tttggtttca tgctttgtga ctatatccag aatacaatgg aataaggata
4801 ttcaggtatt aggacctgta atagactgga atgttactca aagagctgtt tatcaaccct
4861 tacagactag aaggattgca cgttccctta gaatgcagca tcctgttcca aaatatgtgg
4921 aggtaaatat gactagtatt ccacaaggtg tatactatga accccatccg gttgcggccg
4981 cggggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
5041 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
5101 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
5161 ggaggtgtgg gaggtttttt cggatcctct agagtcgacc tgcaggcatg caagcttggc
5221 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
5281 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
5341 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
5401 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
5461 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
5521 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
5581 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
5641 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
5701 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
5761 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
5821 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
5881 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
5941 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
6001 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
6061 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
6121 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
6181 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
6241 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
6301 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
6361 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
6421 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
6481 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
6541 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
6601 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
6661 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
6721 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
6781 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
6841 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
6901 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
6961 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
7021 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
7081 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
7141 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
7201 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
7261 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
7321 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
7381 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
7441 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
7501 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
7561 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
7621 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
7681 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
7741 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
7801 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagt
//
