质粒名称: | pPD49_83 |
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出品公司: | 爱迪基因 |
目录编号: | 1448 |
存储实验室: | Andrew Fire |
载体骨架基本信息 |
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载体名称: | -- |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | 3826 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 蠕虫表达式 |
筛选标记: | -- |
高拷贝/低拷贝: | -- |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | hsp16-25', hsp16-415', IVSA, rf2128 |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pPD49_83 was a gift from Andrew Fire (Addgene plasmid # 1448 ; http://n2t.net/addgene:1448 ; RRID:Addgene_1448) |
买家导航
pPD4983载体基本信息
pPD4983载体图谱质粒图谱和多克隆位点信息
pPD4983载体简介
pPD4983载体序列
LOCUS pPD49_83 3818 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pPD49_83 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3818) AUTHORS Fire A et al TITLE Fire Lab C. Elegans Vector Kit 1995 REFERENCE 2 (bases 1 to 3818) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3818 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(1392..1414) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 1514..1533 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(1611..1629) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1697..1801 /gene="bla" /label=AmpR promoter CDS 1802..2662 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2020..2039) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2833..3421 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3322..3341 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3575..3592 /label=L4440 /note="L4440 vector, forward primer" protein_bind 3709..3730 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3745..3775 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3783..3799 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3788..3810 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 3807..5 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 3807..5 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tcaccaaaaa 61 cggaacgttg agctggacgg aaatagtggt aaagtgacat gattatagtt tgaagatttc 121 taatttcaca attagagcaa atgttgttcg gtatttattt tcaacggtat ttatactatt 181 ttccaccttt ttctagaaca ttcgagctgc ttgttgcaaa aggagggcga ctcacattcg 241 gtacatggaa aagtagtgta cacaataaag agacccagat acattttccg tctgcgtctc 301 tttgcaccca ccgggagtat tttcaaacga atgcatctag gaccttctag aacattctgt 361 aaggctgcag aatgcgggta tataaggaaa gcgggctcag aggaagccaa cacgctttgt 421 tctagtgcat ctaaaaaact tcgaaaatcc tcatcggatc cccgggattg gccaaaggac 481 ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga ggacccttgg 541 ctagcgtcga cggtaccatg gtattgatat ctgagctccg catcggccgc tgtcatcaga 601 tcgccatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca 661 tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa 721 tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa 781 aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa 841 taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg 901 ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac 961 gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa 1021 atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt 1081 ttatgaaatt aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt 1141 tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga 1201 tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa 1261 gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa 1321 acataactgt ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat 1381 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 1441 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 1501 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa 1561 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat 1621 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac 1681 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 1741 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 1801 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 1861 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 1921 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 1981 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 2041 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 2101 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 2161 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 2221 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 2281 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 2341 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 2401 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 2461 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 2521 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 2581 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 2641 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 2701 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 2761 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 2821 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 2881 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 2941 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 3001 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 3061 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 3121 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 3181 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac 3241 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 3301 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 3361 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 3421 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 3481 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 3541 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 3601 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 3661 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 3721 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 3781 aattgtgagc ggataacaat ttcacacagg aaacagct //