质粒名称: | pAdEasy-2 |
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出品公司: | 爱迪基因 |
目录编号: | 16401 |
存储实验室: | Bert Vogelstein |
载体骨架基本信息 |
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载体名称: | pAdEasy-2 |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | 30767 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳动物表达,腺病毒 |
筛选标记: | -- |
高拷贝/低拷贝: | 低拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | AY370909 |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pAdEasy-2 was a gift from Bert Vogelstein (Addgene plasmid # 16401 ; http://n2t.net/addgene:16401 ; RRID:Addgene_16401) |
参考文献部分: | A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916 |
买家导航
pAdEasy2载体基本信息
pAdEasy2载体图谱质粒图谱和多克隆位点信息
pAdEasy2载体简介
pAdEasy2载体序列
LOCUS pAdEasy-2 30829 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Adenoviral vector for use in AdEasy System, for use with large inserts.. ACCESSION . VERSION . KEYWORDS pAdEasy-2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 30829) AUTHORS He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B TITLE A simplified system for generating recombinant adenoviruses. JOURNAL Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. PUBMED 9482916 REFERENCE 2 (bases 1 to 30829) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..30829 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 3262..3365 /label=Ad SA /note="adenovirus major late transcript splice acceptor (Friedrich and Soriano, 1991)" CDS complement(10458..10469) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" regulatory 17941..17950 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" repeat_region 26763..26865 /label=ITR /note="inverted terminal repeat of human adenovirus serotype 5" CDS 28156..28347 /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" primer_bind complement(28341..28363) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 28449..28589 /label=bom /note="basis of mobility region from pBR322" primer_bind 28504..28523 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(28604..28621) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(28775..29363) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(28855..28874) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(29534..30394) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 30157..30176 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(30395..30499) /gene="bla" /label=AmpR promoter ORIGIN 1 ctgcagcctc cgccgccgct tcagccgctg cagccaccgc ccgcgggatt gtgactgact 61 ttgctttcct gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc cgcgatgaca 121 agttgacggc tcttttggca caattggatt ctttgacccg ggaacttaat gtcgtttctc 181 agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc cctcccaatg 241 cggtttaaaa cataaataaa aaaccagact ctgtttggat ttggatcaag caagtgtctt 301 gctgtcttta tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg tctcggtcgt 361 tgagggtcct gtgtattttt tccaggacgt ggtaaaggtg actctggatg ttcagataca 421 tgggcataag cccgtctctg gggtggaggt agcaccactg cagagcttca tgctgcgggg 481 tggtgttgta gatgatccag tcgtagcagg agcgctgggc gtggtgccta aaaatgtctt 541 tcagtagcaa gctgattgcc aggggcaggc ccttggtgta agtgtttaca aagcggttaa 601 gctgggatgg gtgcatacgt ggggatatga gatgcatctt ggactgtatt tttaggttgg 661 ctatgttccc agccatatcc ctccggggat tcatgttgtg cagaaccacc agcacagtgt 721 atccggtgca cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg aagaacttgg 781 agacgccctt gtgacctcca agattttcca tgcattcgtc cataatgatg gcaatgggcc 841 cacgggcggc ggcctgggcg aagatatttc tgggatcact aacgtcatag ttgtgttcca 901 ggatgagatc gtcataggcc atttttacaa agcgcgggcg gagggtgcca gactgcggta 961 taatggttcc atccggccca ggggcgtagt taccctcaca gatttgcatt tcccacgctt 1021 tgagttcaga tggggggatc atgtctacct gcggggcgat gaagaaaacg gtttccgggg 1081 taggggagat cagctgggaa gaaagcaggt tcctgagcag ctgcgactta ccgcagccgg 1141 tgggcccgta aatcacacct attaccggct gcaactggta gttaagagag ctgcagctgc 1201 cgtcatccct gagcaggggg gccacttcgt taagcatgtc cctgactcgc atgttttccc 1261 tgaccaaatc cgccagaagg cgctcgccgc ccagcgatag cagttcttgc aaggaagcaa 1321 agtttttcaa cggtttgaga ccgtccgccg taggcatgct tttgagcgtt tgaccaagca 1381 gttccaggcg gtcccacagc tcggtcacct gctctacggc atctcgatcc agcatatctc 1441 ctcgtttcgc gggttggggc ggctttcgct gtacggcagt agtcggtgct cgtccagacg 1501 ggccagggtc atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct gggtcacggt 1561 gaaggggtgc gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg tcctgctggt 1621 gctgaagcgc tgccggtctt cgccctgcgc gtcggccagg tagcatttga ccatggtgtc 1681 atagtccagc ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg aggaggcgcc 1741 gcacgagggg cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa ataccgattc 1801 cggggagtag gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca cgagccaggt 1861 gagctctggc cgttcggggt caaaaaccag gtttccccca tgctttttga tgcgtttctt 1921 acctctggtt tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt ccgtgtcccc 1981 gtatacagac ttgagaggcc tgtcctcgag cggtgttccg cggtcctcct cgtatagaaa 2041 ctcggaccac tctgagacaa aggctcgcgt ccaggccagc acgaaggagg ctaagtggga 2101 ggggtagcgg tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa gacacatgtc 2161 gccctcttcg gcatcaagga aggtgattgg tttgtaggtg taggccacgt gaccgggtgt 2221 tcctgaaggg gggctataaa agggggtggg ggcgcgttcg tcctcactct cttccgcatc 2281 gctgtctgcg agggccagct gttggggtga gtactccctc tgaaaagcgg gcatgacttc 2341 tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt 2401 gatgcctttg agggtggccg catccatctg gtcagaaaag acaatctttt tgttgtcaag 2461 cttggtggca aacgacccgt agagggcgtt ggacagcaac ttggcgatgg agcgcagggt 2521 ttggtttttg tcgcgatcgg cgcgctcctt ggccgcgatg tttagctgca cgtattcgcg 2581 cgcaacgcac cgccattcgg gaaagacggt ggtgcgctcg tcgggcacca ggtgcacgcg 2641 ccaaccgcgg ttgtgcaggg tgacaaggtc aacgctggtg gctacctctc cgcgtaggcg 2701 ctcgttggtc cagcagaggc ggccgccctt gcgcgagcag aatggcggta gggggtctag 2761 ctgcgtctcg tccggggggt ctgcgtccac ggtaaagacc ccgggcagca ggcgcgcgtc 2821 gaagtagtct atcttgcatc cttgcaagtc tagcgcctgc tgccatgcgc gggcggcaag 2881 cgcgcgctcg tatgggttga gtgggggacc ccatggcatg gggtgggtga gcgcggaggc 2941 gtacatgccg caaatgtcgt aaacgtagag gggctctctg agtattccaa gatatgtagg 3001 gtagcatctt ccaccgcgga tgctggcgcg cacgtaatcg tatagttcgt gcgagggagc 3061 gaggaggtcg ggaccgaggt tgctacgggc gggctgctct gctcggaaga ctatctgcct 3121 gaagatggca tgtgagttgg atgatatggt tggacgctgg aagacgttga agctggcgtc 3181 tgtgagacct accgcgtcac gcacgaagga ggcgtaggag tcgcgcagct tgttgaccag 3241 ctcggcggtg acctgcacgt ctagggcgca gtagtccagg gtttccttga tgatgtcata 3301 cttatcctgt cccttttttt tccacagctc gcggttgagg acaaactctt cgcggtcttt 3361 ccagtactct tggatcggaa acccgtcggc ctccgaacgg taagagccta gcatgtagaa 3421 ctggttgacg gcctggtagg cgcagcatcc cttttctacg ggtagcgcgt atgcctgcgc 3481 ggccttccgg agcgaggtgt gggtgagcgc aaaggtgtcc ctgaccatga ctttgaggta 3541 ctggtatttg aagtcagtgt cgtcgcatcc gccctgctcc cagagcaaaa agtccgtgcg 3601 ctttttggaa cgcggatttg gcagggcgaa ggtgacatcg ttgaagagta tctttcccgc 3661 gcgaggcata aagttgcgtg tgatgcggaa gggtcccggc acctcggaac ggttgttaat 3721 tacctgggcg gcgagcacga tctcgtcaaa gccgttgatg ttgtggccca caatgtaaag 3781 ttccaagaag cgcgggatgc ccttgatgga aggcaatttt ttaagttcct cgtaggtgag 3841 ctcttcaggg gagctgagcc cgtgctctga aagggcccag tctgcaagat gagggttgga 3901 agcgacgaat gagctccaca ggtcacgggc cattagcatt tgcaggtggt cgcgaaaggt 3961 cctaaactgg cgacctatgg ccattttttc tggggtgatg cagtagaagg taagcgggtc 4021 ttgttcccag cggtcccatc caaggttcgc ggctaggtct cgcgcggcag tcactagagg 4081 ctcatctccg ccgaacttca tgaccagcat gaagggcacg agctgcttcc caaaggcccc 4141 catccaagta taggtctcta catcgtaggt gacaaagaga cgctcggtgc gaggatgcga 4201 gccgatcggg aagaactgga tctcccgcca ccaattggag gagtggctat tgatgtggtg 4261 aaagtagaag tccctgcgac gggccgaaca ctcgtgctgg cttttgtaaa aacgtgcgca 4321 gtactggcag cggtgcacgg gctgtacatc ctgcacgagg ttgacctgac gaccgcgcac 4381 aaggaagcag agtgggaatt tgagcccctc gcctggcggg tttggctggt ggtcttctac 4441 ttcggctgct tgtccttgac cgtctggctg ctcgagggga gttacggtgg atcggaccac 4501 cacgccgcgc gagcccaaag tccagatgtc cgcgcgcggc ggtcggagct tgatgacaac 4561 atcgcgcaga tgggagctgt ccatggtctg gagctcccgc ggcgtcaggt caggcgggag 4621 ctcctgcagg tttacctcgc atagacgggt cagggcgcgg gctagatcca ggtgatacct 4681 aatttccagg ggctggttgg tggcggcgtc gatggcttgc aagaggccgc atccccgcgg 4741 cgcgactacg gtaccgcgcg gcgggcggtg ggccgcgggg gtgtccttgg atgatgcatc 4801 taaaagcggt gacgcgggcg agcccccgga ggtagggggg gctccggacc cgccgggaga 4861 gggggcaggg gcacgtcggc gccgcgcgcg ggcaggagct ggtgctgcgc gcgtaggttg 4921 ctggcgaacg cgacgacgcg gcggttgatc tcctgaatct ggcgcctctg cgtgaagacg 4981 acgggcccgg tgagcttgaa cctgaaagag agttcgacag aatcaatttc ggtgtcgttg 5041 acggcggcct ggcgcaaaat ctcctgcacg tctcctgagt tgtcttgata ggcgatctcg 5101 gccatgaact gctcgatctc ttcctcctgg agatctccgc gtccggctcg ctccacggtg 5161 gcggcgaggt cgttggaaat gcgggccatg agctgcgaga aggcgttgag gcctccctcg 5221 ttccagacgc ggctgtagac cacgccccct tcggcatcgc gggcgcgcat gaccacctgc 5281 gcgagattga gctccacgtg ccgggcgaag acggcgtagt ttcgcaggcg ctgaaagagg 5341 tagttgaggg tggtggcggt gtgttctgcc acgaagaagt acataaccca gcgtcgcaac 5401 gtggattcgt tgatatcccc caaggcctca aggcgctcca tggcctcgta gaagtccacg 5461 gcgaagttga aaaactggga gttgcgcgcc gacacggtta actcctcctc cagaagacgg 5521 atgagctcgg cgacagtgtc gcgcacctcg cgctcaaagg ctacaggggc ctcttcttct 5581 tcttcaatct cctcttccat aagggcctcc ccttcttctt cttctggcgg cggtggggga 5641 ggggggacac ggcggcgacg acggcgcacc gggaggcggt cgacaaagcg ctcgatcatc 5701 tccccgcggc gacggcgcat ggtctcggtg acggcgcggc cgttctcgcg ggggcgcagt 5761 tggaagacgc cgcccgtcat gtcccggtta tgggttggcg gggggctgcc atgcggcagg 5821 gatacggcgc taacgatgca tctcaacaat tgttgtgtag gtactccgcc gccgagggac 5881 ctgagcgagt ccgcatcgac cggatcggaa aacctctcga gaaaggcgtc taaccagtca 5941 cagtcgcaag gtaggctgag caccgtggcg ggcggcagcg ggcggcggtc ggggttgttt 6001 ctggcggagg tgctgctgat gatgtaatta aagtaggcgg tcttgagacg gcggatggtc 6061 gacagaagca ccatgtcctt gggtccggcc tgctgaatgc gcaggcggtc ggccatgccc 6121 caggcttcgt tttgacatcg gcgcaggtct ttgtagtagt cttgcatgag cctttctacc 6181 ggcacttctt cttctccttc ctcttgtcct gcatctcttg catctatcgc tgcggcggcg 6241 gcggagtttg gccgtaggtg gcgccctctt cctcccatgc gtgtgacccc gaagcccctc 6301 atcggctgaa gcagggctag gtcggcgaca acgcgctcgg ctaatatggc ctgctgcacc 6361 tgcgtgaggg tagactggaa gtcatccatg tccacaaagc ggtggtatgc gcccgtgttg 6421 atggtgtaag tgcagttggc cataacggac cagttaacgg tctggtgacc cggctgcgag 6481 agctcggtgt acctgagacg cgagtaagcc ctcgagtcaa atacgtagtc gttgcaagtc 6541 cgcaccaggt actggtatcc caccaaaaag tgcggcggcg gctggcggta gaggggccag 6601 cgtagggtgg ccggggctcc gggggcgaga tcttccaaca taaggcgatg atatccgtag 6661 atgtacctgg acatccaggt gatgccggcg gcggtggtgg aggcgcgcgg aaagtcgcgg 6721 acgcggttcc agatgttgcg cagcggcaaa aagtgctcca tggtcgggac gctctggccg 6781 gtcaggcgcg cgcaatcgtt gacgctctag cgtgcaaaag gagagcctgt aagcgggcac 6841 tcttccgtgg tctggtggat aaattcgcaa gggtatcatg gcggacgacc ggggttcgag 6901 ccccgtatcc ggccgtccgc cgtgatccat gcggttaccg cccgcgtgtc gaacccaggt 6961 gtgcgacgtc agacaacggg ggagtgctcc ttttggcttc cttccaggcg cggcggctgc 7021 tgcgctagct tttttggcca ctggccgcgc gcagcgtaag cggttaggct ggaaagcgaa 7081 agcattaagt ggctcgctcc ctgtagccgg agggttattt tccaagggtt gagtcgcggg 7141 acccccggtt cgagtctcgg accggccgga ctgcggcgaa cgggggtttg cctccccgtc 7201 atgcaagacc ccgcttgcaa attcctccgg aaacagggac gagccccttt tttgcttttc 7261 ccagatgcat ccggtgctgc ggcagatgcg cccccctcct cagcagcggc aagagcaaga 7321 gcagcggcag acatgcaggg caccctcccc tcctcctacc gcgtcaggag gggcgacatc 7381 cgcggttgac gcggcagcag atggtgatta cgaacccccg cggcgccggg cccggcacta 7441 cctggacttg gaggagggcg agggcctggc gcggctagga gcgccctctc ctgagcggca 7501 cccaagggtg cagctgaagc gtgatacgcg tgaggcgtac gtgccgcggc agaacctgtt 7561 tcgcgaccgc gagggagagg agcccgagga gatgcgggat cgaaagttcc acgcagggcg 7621 cgagctgcgg catggcctga atcgcgagcg gttgctgcgc gaggaggact ttgagcccga 7681 cgcgcgaacc gggattagtc ccgcgcgcgc acacgtggcg gccgccgacc tggtaaccgc 7741 atacgagcag acggtgaacc aggagattaa ctttcaaaaa agctttaaca accacgtgcg 7801 tacgcttgtg gcgcgcgagg aggtggctat aggactgatg catctgtggg actttgtaag 7861 cgcgctggag caaaacccaa atagcaagcc gctcatggcg cagctgttcc ttatagtgca 7921 gcacagcagg gacaacgagg cattcaggga tgcgctgcta aacatagtag agcccgaggg 7981 ccgctggctg ctcgatttga taaacatcct gcagagcata gtggtgcagg agcgcagctt 8041 gagcctggct gacaaggtgg ccgccatcaa ctattccatg cttagcctgg gcaagtttta 8101 cgcccgcaag atataccata ccccttacgt tcccatagac aaggaggtaa agatcgaggg 8161 gttctacatg cgcatggcgc tgaaggtgct taccttgagc gacgacctgg gcgtttatcg 8221 caacgagcgc atccacaagg ccgtgagcgt gagccggcgg cgcgagctca gcgaccgcga 8281 gctgatgcac agcctgcaaa gggccctggc tggcacgggc agcggcgata gagaggccga 8341 gtcctacttt gacgcgggcg ctgacctgcg ctgggcccca agccgacgcg ccctggaggc 8401 agctggggcc ggacctgggc tggcggtggc acccgcgcgc gctggcaacg tcggcggcgt 8461 ggaggaatat gacgaggacg atgagtacga gccagaggac ggcgagtact aagcggtgat 8521 gtttctgatc agatgatgca agacgcaacg gacccggcgg tgcgggcggc gctgcagagc 8581 cagccgtccg gccttaactc cacggacgac tggcgccagg tcatggaccg catcatgtcg 8641 ctgactgcgc gcaatcctga cgcgttccgg cagcagccgc aggccaaccg gctctccgca 8701 attctggaag cggtggtccc ggcgcgcgca aaccccacgc acgagaaggt gctggcgatc 8761 gtaaacgcgc tggccgaaaa cagggccatc cggcccgacg aggccggcct ggtctacgac 8821 gcgctgcttc agcgcgtggc tcgttacaac agcggcaacg tgcagaccaa cctggaccgg 8881 ctggtggggg atgtgcgcga ggccgtggcg cagcgtgagc gcgcgcagca gcagggcaac 8941 ctgggctcca tggttgcact aaacgccttc ctgagtacac agcccgccaa cgtgccgcgg 9001 ggacaggagg actacaccaa ctttgtgagc gcactgcggc taatggtgac tgagacaccg 9061 caaagtgagg tgtaccagtc tgggccagac tattttttcc agaccagtag acaaggcctg 9121 cagaccgtaa acctgagcca ggctttcaaa aacttgcagg ggctgtgggg ggtgcgggct 9181 cccacaggcg accgcgcgac cgtgtctagc ttgctgacgc ccaactcgcg cctgttgctg 9241 ctgctaatag cgcccttcac ggacagtggc agcgtgtccc gggacacata cctaggtcac 9301 ttgctgacac tgtaccgcga ggccataggt caggcgcatg tggacgagca tactttccag 9361 gagattacaa gtgtcagccg cgcgctgggg caggaggaca cgggcagcct ggaggcaacc 9421 ctaaactacc tgctgaccaa ccggcggcag aagatcccct cgttgcacag tttaaacagc 9481 gaggaggagc gcattttgcg ctacgtgcag cagagcgtga gccttaacct gatgcgcgac 9541 ggggtaacgc ccagcgtggc gctggacatg accgcgcgca acatggaacc gggcatgtat 9601 gcctcaaacc ggccgtttat caaccgccta atggactact tgcatcgcgc ggccgccgtg 9661 aaccccgagt atttcaccaa tgccatcttg aacccgcact ggctaccgcc ccctggtttc 9721 tacaccgggg gattcgaggt gcccgagggt aacgatggat tcctctggga cgacatagac 9781 gacagcgtgt tttccccgca accgcagacc ctgctagagt tgcaacagcg cgagcaggca 9841 gaggcggcgc tgcgaaagga aagcttccgc aggccaagca gcttgtccga tctaggcgct 9901 gcggccccgc ggtcagatgc tagtagccca tttccaagct tgatagggtc tcttaccagc 9961 actcgcacca cccgcccgcg cctgctgggc gaggaggagt acctaaacaa ctcgctgctg 10021 cagccgcagc gcgaaaaaaa cctgcctccg gcatttccca acaacgggat agagagccta 10081 gtggacaaga tgagtagatg gaagacgtac gcgcaggagc acagggacgt gccaggcccg 10141 cgcccgccca cccgtcgtca aaggcacgac cgtcagcggg gtctggtgtg ggaggacgat 10201 gactcggcag acgacagcag cgtcctggat ttgggaggga gtggcaaccc gtttgcgcac 10261 cttcgcccca ggctggggag aatgttttaa aaaaaaaaaa gcatgatgca aaataaaaaa 10321 ctcaccaagg ccatggcacc gagcgttggt tttcttgtat tccccttagt atgcggcgcg 10381 cggcgatgta tgaggaaggt cctcctccct cctacgagag tgtggtgagc gcggcgccag 10441 tggcggcggc gctgggttct cccttcgatg ctcccctgga cccgccgttt gtgcctccgc 10501 ggtacctgcg gcctaccggg gggagaaaca gcatccgtta ctctgagttg gcacccctat 10561 tcgacaccac ccgtgtgtac ctggtggaca acaagtcaac ggatgtggca tccctgaact 10621 accagaacga ccacagcaac tttctgacca cggtcattca aaacaatgac tacagcccgg 10681 gggaggcaag cacacagacc atcaatcttg acgaccggtc gcactggggc ggcgacctga 10741 aaaccatcct gcataccaac atgccaaatg tgaacgagtt catgtttacc aataagttta 10801 aggcgcgggt gatggtgtcg cgcttgccta ctaaggacaa tcaggtggag ctgaaatacg 10861 agtgggtgga gttcacgctg cccgagggca actactccga gaccatgacc atagacctta 10921 tgaacaacgc gatcgtggag cactacttga aagtgggcag acagaacggg gttctggaaa 10981 gcgacatcgg ggtaaagttt gacacccgca acttcagact ggggtttgac cccgtcactg 11041 gtcttgtcat gcctggggta tatacaaacg aagccttcca tccagacatc attttgctgc 11101 caggatgcgg ggtggacttc acccacagcc gcctgagcaa cttgttgggc atccgcaagc 11161 ggcaaccctt ccaggagggc tttaggatca cctacgatga tctggagggt ggtaacattc 11221 ccgcactgtt ggatgtggac gcctaccagg cgagcttgaa agatgacacc gaacagggcg 11281 ggggtggcgc aggcggcagc aacagcagtg gcagcggcgc ggaagagaac tccaacgcgg 11341 cagccgcggc aatgcagccg gtggaggaca tgaacgatca tgccattcgc ggcgacacct 11401 ttgccacacg ggctgaggag aagcgcgctg aggccgaagc agcggccgaa gctgccgccc 11461 ccgctgcgca acccgaggtc gagaagcctc agaagaaacc ggtgatcaaa cccctgacag 11521 aggacagcaa gaaacgcagt tacaacctaa taagcaatga cagcaccttc acccagtacc 11581 gcagctggta ccttgcatac aactacggcg accctcagac cggaatccgc tcatggaccc 11641 tgctttgcac tcctgacgta acctgcggct cggagcaggt ctactggtcg ttgccagaca 11701 tgatgcaaga ccccgtgacc ttccgctcca cgcgccagat cagcaacttt ccggtggtgg 11761 gcgccgagct gttgcccgtg cactccaaga gcttctacaa cgaccaggcc gtctactccc 11821 aactcatccg ccagtttacc tctctgaccc acgtgttcaa tcgctttccc gagaaccaga 11881 ttttggcgcg cccgccagcc cccaccatca ccaccgtcag tgaaaacgtt cctgctctca 11941 cagatcacgg gacgctaccg ctgcgcaaca gcatcggagg agtccagcga gtgaccatta 12001 ctgacgccag acgccgcacc tgcccctacg tttacaaggc cctgggcata gtctcgccgc 12061 gcgtcctatc gagccgcact ttttgagcaa gcatgtccat ccttatatcg cccagcaata 12121 acacaggctg gggcctgcgc ttcccaagca agatgtttgg cggggccaag aagcgctccg 12181 accaacaccc agtgcgcgtg cgcgggcact accgcgcgcc ctggggcgcg cacaaacgcg 12241 gccgcactgg gcgcaccacc gtcgatgacg ccatcgacgc ggtggtggag gaggcgcgca 12301 actacacgcc cacgccgcca ccagtgtcca cagtggacgc ggccattcag accgtggtgc 12361 gcggagcccg gcgctatgct aaaatgaaga gacggcggag gcgcgtagca cgtcgccacc 12421 gccgccgacc cggcactgcc gcccaacgcg cggcggcggc cctgcttaac cgcgcacgtc 12481 gcaccggccg acgggcggcc atgcgggccg ctcgaaggct ggccgcgggt attgtcactg 12541 tgccccccag gtccaggcga cgagcggccg ccgcagcagc cgcggccatt agtgctatga 12601 ctcagggtcg caggggcaac gtgtattggg tgcgcgactc ggttagcggc ctgcgcgtgc 12661 ccgtgcgcac ccgccccccg cgcaactaga ttgcaagaaa aaactactta gactcgtact 12721 gttgtatgta tccagcggcg gcggcgcgca acgaagctat gtccaagcgc aaaatcaaag 12781 aagagatgct ccaggtcatc gcgccggaga tctatggccc cccgaagaag gaagagcagg 12841 attacaagcc ccgaaagcta aagcgggtca aaaagaaaaa gaaagatgat gatgatgaac 12901 ttgacgacga ggtggaactg ctgcacgcta ccgcgcccag gcgacgggta cagtggaaag 12961 gtcgacgcgt aaaacgtgtt ttgcgacccg gcaccaccgt agtctttacg cccggtgagc 13021 gctccacccg cacctacaag cgcgtgtatg atgaggtgta cggcgacgag gacctgcttg 13081 agcaggccaa cgagcgcctc ggggagtttg cctacggaaa gcggcataag gacatgctgg 13141 cgttgccgct ggacgagggc aacccaacac ctagcctaaa gcccgtaaca ctgcagcagg 13201 tgctgcccgc gcttgcaccg tccgaagaaa agcgcggcct aaagcgcgag tctggtgact 13261 tggcacccac cgtgcagctg atggtaccca agcgccagcg actggaagat gtcttggaaa 13321 aaatgaccgt ggaacctggg ctggagcccg aggtccgcgt gcggccaatc aagcaggtgg 13381 cgccgggact gggcgtgcag accgtggacg ttcagatacc cactaccagt agcaccagta 13441 ttgccaccgc cacagagggc atggagacac aaacgtcccc ggttgcctca gcggtggcgg 13501 atgccgcggt gcaggcggtc gctgcggccg cgtccaagac ctctacggag gtgcaaacgg 13561 acccgtggat gtttcgcgtt tcagcccccc ggcgcccgcg ccgttcgagg aagtacggcg 13621 ccgccagcgc gctactgccc gaatatgccc tacatccttc cattgcgcct acccccggct 13681 atcgtggcta cacctaccgc cccagaagac gagcaactac ccgacgccga accaccactg 13741 gaacccgccg ccgccgtcgc cgtcgccagc ccgtgctggc cccgatttcc gtgcgcaggg 13801 tggctcgcga aggaggcagg accctggtgc tgccaacagc gcgctaccac cccagcatcg 13861 tttaaaagcc ggtctttgtg gttcttgcag atatggccct cacctgccgc ctccgtttcc 13921 cggtgccggg attccgagga agaatgcacc gtaggagggg catggccggc cacggcctga 13981 cgggcggcat gcgtcgtgcg caccaccggc ggcggcgcgc gtcgcaccgt cgcatgcgcg 14041 gcggtatcct gcccctcctt attccactga tcgccgcggc gattggcgcc gtgcccggaa 14101 ttgcatccgt ggccttgcag gcgcagagac actgattaaa aacaagttgc atgtggaaaa 14161 atcaaaataa aaagtctgga ctctcacgct cgcttggtcc tgtaactatt ttgtagaatg 14221 gaagacatca actttgcgtc tctggccccg cgacacggct cgcgcccgtt catgggaaac 14281 tggcaagata tcggcaccag caatatgagc ggtggcgcct tcagctgggg ctcgctgtgg 14341 agcggcatta aaaatttcgg ttccaccgtt aagaactatg gcagcaaggc ctggaacagc 14401 agcacaggcc agatgctgag ggataagttg aaagagcaaa atttccaaca aaaggtggta 14461 gatggcctgg cctctggcat tagcggggtg gtggacctgg ccaaccaggc agtgcaaaat 14521 aagattaaca gtaagcttga tccccgccct cccgtagagg agcctccacc ggccgtggag 14581 acagtgtctc cagaggggcg tggcgaaaag cgtccgcgcc ccgacaggga agaaactctg 14641 gtgacgcaaa tagacgagcc tccctcgtac gaggaggcac taaagcaagg cctgcccacc 14701 acccgtccca tcgcgcccat ggctaccgga gtgctgggcc agcacacacc cgtaacgctg 14761 gacctgcctc cccccgccga cacccagcag aaacctgtgc tgccaggccc gaccgccgtt 14821 gttgtaaccc gtcctagccg cgcgtccctg cgccgcgccg ccagcggtcc gcgatcgttg 14881 cggcccgtag ccagtggcaa ctggcaaagc acactgaaca gcatcgtggg tctgggggtg 14941 caatccctga agcgccgacg atgcttctga tagctaacgt gtcgtatgtg tgtcatgtat 15001 gcgtccatgt cgccgccaga ggagctgctg agccgccgcg cgcccgcttt ccaagatggc 15061 taccccttcg atgatgccgc agtggtctta catgcacatc tcgggccagg acgcctcgga 15121 gtacctgagc cccgggctgg tgcagtttgc ccgcgccacc gagacgtact tcagcctgaa 15181 taacaagttt agaaacccca cggtggcgcc tacgcacgac gtgaccacag accggtccca 15241 gcgtttgacg ctgcggttca tccctgtgga ccgtgaggat actgcgtact cgtacaaggc 15301 gcggttcacc ctagctgtgg gtgataaccg tgtgctggac atggcttcca cgtactttga 15361 catccgcggc gtgctggaca ggggccctac ttttaagccc tactctggca ctgcctacaa 15421 cgccctggct cccaagggtg ccccaaatcc ttgcgaatgg gatgaagctg ctactgctct 15481 tgaaataaac ctagaagaag aggacgatga caacgaagac gaagtagacg agcaagctga 15541 gcagcaaaaa actcacgtat ttgggcaggc gccttattct ggtataaata ttacaaagga 15601 gggtattcaa ataggtgtcg aaggtcaaac acctaaatat gccgataaaa catttcaacc 15661 tgaacctcaa ataggagaat ctcagtggta cgaaacagaa attaatcatg cagctgggag 15721 agtcctaaaa aagactaccc caatgaaacc atgttacggt tcatatgcaa aacccacaaa 15781 tgaaaatgga gggcaaggca ttcttgtaaa gcaacaaaat ggaaagctag aaagtcaagt 15841 ggaaatgcaa tttttctcaa ctactgaggc agccgcaggc aatggtgata acttgactcc 15901 taaagtggta ttgtacagtg aagatgtaga tatagaaacc ccagacactc atatttctta 15961 catgcccact attaaggaag gtaactcacg agaactaatg ggccaacaat ctatgcccaa 16021 caggcctaat tacattgctt ttagggacaa ttttattggt ctaatgtatt acaacagcac 16081 gggtaatatg ggtgttctgg cgggccaagc atcgcagttg aatgctgttg tagatttgca 16141 agacagaaac acagagcttt cataccagct tttgcttgat tccattggtg atagaaccag 16201 gtacttttct atgtggaatc aggctgttga cagctatgat ccagatgtta gaattattga 16261 aaatcatgga actgaagatg aacttccaaa ttactgcttt ccactgggag gtgtgattaa 16321 tacagagact cttaccaagg taaaacctaa aacaggtcag gaaaatggat gggaaaaaga 16381 tgctacagaa ttttcagata aaaatgaaat aagagttgga aataattttg ccatggaaat 16441 caatctaaat gccaacctgt ggagaaattt cctgtactcc aacatagcgc tgtatttgcc 16501 cgacaagcta aagtacagtc cttccaacgt aaaaatttct gataacccaa acacctacga 16561 ctacatgaac aagcgagtgg tggctcccgg gctagtggac tgctacatta accttggagc 16621 acgctggtcc cttgactata tggacaacgt caacccattt aaccaccacc gcaatgctgg 16681 cctgcgctac cgctcaatgt tgctgggcaa tggtcgctat gtgcccttcc acatccaggt 16741 gcctcagaag ttctttgcca ttaaaaacct ccttctcctg ccgggctcat acacctacga 16801 gtggaacttc aggaaggatg ttaacatggt tctgcagagc tccctaggaa atgacctaag 16861 ggttgacgga gccagcatta agtttgatag catttgcctt tacgccacct tcttccccat 16921 ggcccacaac accgcctcca cgcttgaggc catgcttaga aacgacacca acgaccagtc 16981 ctttaacgac tatctctccg ccgccaacat gctctaccct atacccgcca acgctaccaa 17041 cgtgcccata tccatcccct cccgcaactg ggcggctttc cgcggctggg ccttcacgcg 17101 ccttaagact aaggaaaccc catcactggg ctcgggctac gacccttatt acacctactc 17161 tggctctata ccctacctag atggaacctt ttacctcaac cacaccttta agaaggtggc 17221 cattaccttt gactcttctg tcagctggcc tggcaatgac cgcctgctta cccccaacga 17281 gtttgaaatt aagcgctcag ttgacgggga gggttacaac gttgcccagt gtaacatgac 17341 caaagactgg ttcctggtac aaatgctagc taactataac attggctacc agggcttcta 17401 tatcccagag agctacaagg accgcatgta ctccttcttt agaaacttcc agcccatgag 17461 ccgtcaggtg gtggatgata ctaaatacaa ggactaccaa caggtgggca tcctacacca 17521 acacaacaac tctggatttg ttggctacct tgcccccacc atgcgcgaag gacaggccta 17581 ccctgctaac ttcccctatc cgcttatagg caagaccgca gttgacagca ttacccagaa 17641 aaagtttctt tgcgatcgca ccctttggcg catcccattc tccagtaact ttatgtccat 17701 gggcgcactc acagacctgg gccaaaacct tctctacgcc aactccgccc acgcgctaga 17761 catgactttt gaggtggatc ccatggacga gcccaccctt ctttatgttt tgtttgaagt 17821 ctttgacgtg gtccgtgtgc accagccgca ccgcggcgtc atcgaaaccg tgtacctgcg 17881 cacgcccttc tcggccggca acgccacaac ataaagaagc aagcaacatc aacaacagct 17941 gccgccatgg gctccagtga gcaggaactg aaagccattg tcaaagatct tggttgtggg 18001 ccatattttt tgggcaccta tgacaagcgc tttccaggct ttgtttctcc acacaagctc 18061 gcctgcgcca tagtcaatac ggccggtcgc gagactgggg gcgtacactg gatggccttt 18121 gcctggaacc cgcactcaaa aacatgctac ctctttgagc cctttggctt ttctgaccag 18181 cgactcaagc aggtttacca gtttgagtac gagtcactcc tgcgccgtag cgccattgct 18241 tcttcccccg accgctgtat aacgctggaa aagtccaccc aaagcgtaca ggggcccaac 18301 tcggccgcct gtggactatt ctgctgcatg tttctccacg cctttgccaa ctggccccaa 18361 actcccatgg atcacaaccc caccatgaac cttattaccg gggtacccaa ctccatgctc 18421 aacagtcccc aggtacagcc caccctgcgt cgcaaccagg aacagctcta cagcttcctg 18481 gagcgccact cgccctactt ccgcagccac agtgcgcaga ttaggagcgc cacttctttt 18541 tgtcacttga aaaacatgta aaaataatgt actagagaca ctttcaataa aggcaaatgc 18601 ttttatttgt acactctcgg gtgattattt acccccaccc ttgccgtctg cgccgtttaa 18661 aaatcaaagg ggttctgccg cgcatcgcta tgcgccactg gcagggacac gttgcgatac 18721 tggtgtttag tgctccactt aaactcaggc acaaccatcc gcggcagctc ggtgaagttt 18781 tcactccaca ggctgcgcac catcaccaac gcgtttagca ggtcgggcgc cgatatcttg 18841 aagtcgcagt tggggcctcc gccctgcgcg cgcgagttgc gatacacagg gttgcagcac 18901 tggaacacta tcagcgccgg gtggtgcacg ctggccagca cgctcttgtc ggagatcaga 18961 tccgcgtcca ggtcctccgc gttgctcagg gcgaacggag tcaactttgg tagctgcctt 19021 cccaaaaagg gcgcgtgccc aggctttgag ttgcactcgc accgtagtgg catcaaaagg 19081 tgaccgtgcc cggtctgggc gttaggatac agcgcctgca taaaagcctt gatctgctta 19141 aaagccacct gagcctttgc gccttcagag aagaacatgc cgcaagactt gccggaaaac 19201 tgattggccg gacaggccgc gtcgtgcacg cagcaccttg cgtcggtgtt ggagatctgc 19261 accacatttc ggccccaccg gttcttcacg atcttggcct tgctagactg ctccttcagc 19321 gcgcgctgcc cgttttcgct cgtcacatcc atttcaatca cgtgctcctt atttatcata 19381 atgcttccgt gtagacactt aagctcgcct tcgatctcag cgcagcggtg cagccacaac 19441 gcgcagcccg tgggctcgtg atgcttgtag gtcacctctg caaacgactg caggtacgcc 19501 tgcaggaatc gccccatcat cgtcacaaag gtcttgttgc tggtgaaggt cagctgcaac 19561 ccgcggtgct cctcgttcag ccaggtcttg catacggccg ccagagcttc cacttggtca 19621 ggcagtagtt tgaagttcgc ctttagatcg ttatccacgt ggtacttgtc catcagcgcg 19681 cgcgcagcct ccatgccctt ctcccacgca gacacgatcg gcacactcag cgggttcatc 19741 accgtaattt cactttccgc ttcgctgggc tcttcctctt cctcttgcgt ccgcatacca 19801 cgcgccactg ggtcgtcttc attcagccgc cgcactgtgc gcttacctcc tttgccatgc 19861 ttgattagca ccggtgggtt gctgaaaccc accatttgta gcgccacatc ttctctttct 19921 tcctcgctgt ccacgattac ctctggtgat ggcgggcgct cgggcttggg agaagggcgc 19981 ttctttttct tcttgggcgc aatggccaaa tccgccgccg aggtcgatgg ccgcgggctg 20041 ggtgtgcgcg gcaccagcgc gtcttgtgat gagtcttcct cgtcctcgga ctcgatacgc 20101 cgcctcatcc gcttttttgg gggcgcccgg ggaggcggcg gcgacgggga cggggacgac 20161 acgtcctcca tggttggggg acgtcgcgcc gcaccgcgtc cgcgctcggg ggtggtttcg 20221 cgctgctcct cttcccgact ggccatttcc ttctcctata ggcagaaaaa gatcatggag 20281 tcagtcgaga agaaggacag cctaaccgcc ccctctgagt tcgccaccac cgcctccacc 20341 gatgccgcca acgcgcctac caccttcccc gtcgaggcac ccccgcttga ggaggaggaa 20401 gtgattatcg agcaggaccc aggttttgta agcgaagacg acgaggaccg ctcagtacca 20461 acagaggata aaaagcaaga ccaggacaac gcagaggcaa acgaggaaca agtcgggcgg 20521 ggggacgaaa ggcatggcga ctacctagat gtgggagacg acgtgctgtt gaagcatctg 20581 cagcgccagt gcgccattat ctgcgacgcg ttgcaagagc gcagcgatgt gcccctcgcc 20641 atagcggatg tcagccttgc ctacgaacgc cacctattct caccgcgcgt accccccaaa 20701 cgccaagaaa acggcacatg cgagcccaac ccgcgcctca acttctaccc cgtatttgcc 20761 gtgccagagg tgcttgccac ctatcacatc tttttccaaa actgcaagat acccctatcc 20821 tgccgtgcca accgcagccg agcggacaag cagctggcct tgcggcaggg cgctgtcata 20881 cctgatatcg cctcgctcaa cgaagtgcca aaaatctttg agggtcttgg acgcgacgag 20941 aagcgcgcgg caaacgctct gcaacaggaa aacagcgaaa atgaaagtca ctctggagtg 21001 ttggtggaac tcgagggtga caacgcgcgc ctagccgtac taaaacgcag catcgaggtc 21061 acccactttg cctacccggc acttaaccta ccccccaagg tcatgagcac agtcatgagt 21121 gagctgatcg tgcgccgtgc gcagcccctg gagagggatg caaatttgca agaacaaaca 21181 gaggagggcc tacccgcagt tggcgacgag cagctagcgc gctggcttca aacgcgcgag 21241 cctgccgact tggaggagcg acgcaaacta atgatggccg cagtgctcgt taccgtggag 21301 cttgagtgca tgcagcggtt ctttgctgac ccggagatgc agcgcaagct agaggaaaca 21361 ttgcactaca cctttcgaca gggctacgta cgccaggcct gcaagatctc caacgtggag 21421 ctctgcaacc tggtctccta ccttggaatt ttgcacgaaa accgccttgg gcaaaacgtg 21481 cttcattcca cgctcaaggg cgaggcgcgc cgcgactacg tccgcgactg cgtttactta 21541 tttctatgct acacctggca gacggccatg ggcgtttggc agcagtgctt ggaggagtgc 21601 aacctcaagg agctgcagaa actgctaaag caaaacttga aggacctatg gacggccttc 21661 aacgagcgct ccgtggccgc gcacctggcg gacatcattt tccccgaacg cctgcttaaa 21721 accctgcaac agggtctgcc agacttcacc agtcaaagca tgttgcagaa ctttaggaac 21781 tttatcctag agcgctcagg aatcttgccc gccacctgct gtgcacttcc tagcgacttt 21841 gtgcccatta agtaccgcga atgccctccg ccgctttggg gccactgcta ccttctgcag 21901 ctagccaact accttgccta ccactctgac ataatggaag acgtgagcgg tgacggtcta 21961 ctggagtgtc actgtcgctg caacctatgc accccgcacc gctccctggt ttgcaattcg 22021 cagctgctta acgaaagtca aattatcggt acctttgagc tgcagggtcc ctcgcctgac 22081 gaaaagtccg cggctccggg gttgaaactc actccggggc tgtggacgtc ggcttacctt 22141 cgcaaatttg tacctgagga ctaccacgcc cacgagatta ggttctacga agaccaatcc 22201 cgcccgccta atgcggagct taccgcctgc gtcattaccc agggccacat tcttggccaa 22261 ttgcaagcca tcaacaaagc ccgccaagag tttctgctac gaaagggacg gggggtttac 22321 ttggaccccc agtccggcga ggagctcaac ccaatccccc cgccgccgca gccctatcag 22381 cagcagccgc gggcccttgc ttcccaggat ggcacccaaa aagaagctgc agctgccgcc 22441 gccacccacg gacgaggagg aatactggga cagtcaggca gaggaggttt tggacgagga 22501 ggaggaggac atgatggaag actgggagag cctagacgag gaagcttccg aggtcgaaga 22561 ggtgtcagac gaaacaccgt caccctcggt cgcattcccc tcgccggcgc cccagaaatc 22621 ggcaaccggt tccagcatgg ctacaacctc cgctcctcag gcgccgccgg cactgcccgt 22681 tcgccgaccc aaccgtagat gggacaccac tggaaccagg gccggtaagt ccaagcagcc 22741 gccgccgtta gcccaagagc aacaacagcg ccaaggctac cgctcatggc gcgggcacaa 22801 gaacgccata gttgcttgct tgcaagactg tgggggcaac atctccttcg cccgccgctt 22861 tcttctctac catcacggcg tggccttccc ccgtaacatc ctgcattact accgtcatct 22921 ctacagccca tactgcaccg gcggcagcgg cagcaacagc agcggccaca cagaagcaaa 22981 ggcgaccgga tagcaagact ctgacaaagc ccaagaaatc cacagcggcg gcagcagcag 23041 gaggaggagc gctgcgtctg gcgcccaacg aacccgtatc gacccgcgag cttagaaaca 23101 ggatttttcc cactctgtat gctatatttc aacagagcag gggccaagaa caagagctga 23161 aaataaaaaa caggtctctg cgatccctca cccgcagctg cctgtatcac aaaagcgaag 23221 atcagcttcg gcgcacgctg gaagacgcgg aggctctctt cagtaaatac tgcgcgctga 23281 ctcttaagga ctagtttcgc gccctttctc aaatttaagc gcgaaaacta cgtcatctcc 23341 agcggccaca cccggcgcca gcacctgttg tcagcgccat tatgagcaag gaaattccca 23401 cgccctacat gtggagttac cagccacaaa tgggacttgc ggctggagct gcccaagact 23461 actcaacccg aataaactac atgagcgcgg gaccccacat gatatcccgg gtcaacggaa 23521 tacgcgccca ccgaaaccga attctcctgg aacaggcggc tattaccacc acacctcgta 23581 ataaccttaa tccccgtagt tggcccgctg ccctggtgta ccaggaaagt cccgctccca 23641 ccactgtggt acttcccaga gacgcccagg ccgaagttca gatgactaac tcaggggcgc 23701 agcttgcggg cggctttcgt cacagggtgc ggtcgcccgg gcagggtata actcacctga 23761 caatcagagg gcgaggtatt cagctcaacg acgagtcggt gagctcctcg cttggtctcc 23821 gtccggacgg gacatttcag atcggcggcg ccggccgctc ttcattcacg cctcgtcagg 23881 caatcctaac tctgcagacc tcgtcctctg agccgcgctc tggaggcatt ggaactctgc 23941 aatttattga ggagtttgtg ccatcggtct actttaaccc cttctcggga cctcccggcc 24001 actatccgga tcaatttatt cctaactttg acgcggtaaa ggactcggcg gacggctacg 24061 actgaatgtt aagtggagag gcagagcaac tgcgcctgaa acacctggtc cactgtcgcc 24121 gccacaagtg ctttgcccgc gactccggtg agttttgcta ctttgaattg cccgaggatc 24181 atatcgaggg cccggcgcac ggcgtccggc ttaccgccca gggagagctt gcccgtagcc 24241 tgattcggga gtttacccag cgccccctgc tagttgagcg ggacagggga ccctgtgttc 24301 tcactgtgat ttgcaactgt cctaaccctg gattacatca agatcctcta gttaatgtca 24361 ggtcgcctaa gtcgattaac tagagtaccc ggggatctta ttccctttaa ctaataaaaa 24421 aaaataataa agcatcactt acttaaaatc agttagcaaa tttctgtcca gtttattcag 24481 cagcacctcc ttgccctcct cccagctctg gtattgcagc ttcctcctgg ctgcaaactt 24541 tctccacaat ctaaatggaa tgtcagtttc ctcctgttcc tgtccatccg cacccactat 24601 cttcatgttg ttgcagatga agcgcgcaag accgtctgaa gataccttca accccgtgta 24661 tccatatgac acggaaaccg gtcctccaac tgtgcctttt cttactcctc cctttgtatc 24721 ccccaatggg tttcaagaga gtccccctgg ggtactctct ttgcgcctat ccgaacctct 24781 agttacctcc aatggcatgc ttgcgctcaa aatgggcaac ggcctctctc tggacgaggc 24841 cggcaacctt acctcccaaa atgtaaccac tgtgagccca cctctcaaaa aaaccaagtc 24901 aaacataaac ctggaaatat ctgcacccct cacagttacc tcagaagccc taactgtggc 24961 tgccgccgca cctctaatgg tcgcgggcaa cacactcacc atgcaatcac aggccccgct 25021 aaccgtgcac gactccaaac ttagcattgc cacccaagga cccctcacag tgtcagaagg 25081 aaagctagcc ctgcaaacat caggccccct caccaccacc gatagcagta cccttactat 25141 cactgcctca ccccctctaa ctactgccac tggtagcttg ggcattgact tgaaagagcc 25201 catttataca caaaatggaa aactaggact aaagtacggg gctcctttgc atgtaacaga 25261 cgacctaaac actttgaccg tagcaactgg tccaggtgtg actattaata atacttcctt 25321 gcaaactaaa gttactggag ccttgggttt tgattcacaa ggcaatatgc aacttaatgt 25381 agcaggagga ctaaggattg attctcaaaa cagacgcctt atacttgatg ttagttatcc 25441 gtttgatgct caaaaccaac taaatctaag actaggacag ggccctcttt ttataaactc 25501 agcccacaac ttggatatta actacaacaa aggcctttac ttgtttacag cttcaaacaa 25561 ttccaaaaag cttgaggtta acctaagcac tgccaagggg ttgatgtttg acgctacagc 25621 catagccatt aatgcaggag atgggcttga atttggttca cctaatgcac caaacacaaa 25681 tcccctcaaa acaaaaattg gccatggcct agaatttgat tcaaacaagg ctatggttcc 25741 taaactagga actggcctta gttttgacag cacaggtgcc attacagtag gaaacaaaaa 25801 taatgataag ctaactttgt ggaccacacc agctccatct cctaactgta gactaaatgc 25861 agagaaagat gctaaactca ctttggtctt aacaaaatgt ggcagtcaaa tacttgctac 25921 agtttcagtt ttggctgtta aaggcagttt ggctccaata tctggaacag ttcaaagtgc 25981 tcatcttatt ataagatttg acgaaaatgg agtgctacta aacaattcct tcctggaccc 26041 agaatattgg aactttagaa atggagatct tactgaaggc acagcctata caaacgctgt 26101 tggatttatg cctaacctat cagcttatcc aaaatctcac ggtaaaactg ccaaaagtaa 26161 cattgtcagt caagtttact taaacggaga caaaactaaa cctgtaacac taaccattac 26221 actaaacggt acacaggaaa caggagacac aactccaagt gcatactcta tgtcattttc 26281 atgggactgg tctggccaca actacattaa tgaaatattt gccacatcct cttacacttt 26341 ttcatacatt gcccaagaat aaagaatcgt ttgtgttatg tttcaacgtg cctaggcaaa 26401 atagcaccct cccgctccag aacaacatac agcgcttcca cagcggcagc cataacagtc 26461 agccttacca gtaaaaaaga aaacctatta aaaaaacacc actcgacacg gcaccagctc 26521 aatcagtcac agtgtaaaaa agggccaagt gcagagcgag tatatatagg actaaaaaat 26581 gacgtaacgg ttaaagtcca caaaaaacac ccagaaaacc gcacgcgaac ctacgcccag 26641 aaacgaaagc caaaaaaccc acaacttcct caaatcgtca cttccgtttt cccacgttac 26701 gtcacttccc attttaagaa aactacaatt cccaacacat acaagttact ccgccctaaa 26761 acctacgtca cccgccccgt tcccacgccc cgcgccacgt cacaaactcc accccctcat 26821 tatcatattg gcttcaatcc aaaataaggt atattattga tgatgttaat taacatgcat 26881 ggatcctacg tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg 26941 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt 27001 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc 27061 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt 27121 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc 27181 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc 27241 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc 27301 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag 27361 cctaacttcg atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac 27421 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt 27481 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct 27541 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg 27601 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg 27661 cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg 27721 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc 27781 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg 27841 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt 27901 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta 27961 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc 28021 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt 28081 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct 28141 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc 28201 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca 28261 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt 28321 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 28381 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 28441 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact 28501 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca 28561 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc 28621 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 28681 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 28741 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 28801 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 28861 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 28921 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 28981 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 29041 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 29101 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 29161 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 29221 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 29281 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 29341 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 29401 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 29461 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 29521 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 29581 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 29641 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 29701 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 29761 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 29821 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg 29881 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 29941 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 30001 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 30061 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 30121 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa 30181 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 30241 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 30301 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 30361 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 30421 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 30481 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgtctag ctacgaattc 30541 ttcgacagct tcgaaactag aatcgatttc gaaactagct taagggtggg aaagaatata 30601 taaggtgggg gtcttatgta gttttgtatc tgttttgcag cagccgccgc cgccatgagc 30661 accaactcgt ttgatggaag cattgtgagc tcatatttga caacgcgcat gcccccatgg 30721 gccggggtgc gtcagaatgt gatgggctcc agcattgatg gtcgccccgt cctgcccgca 30781 aactctacta ccttgaccta cgagaccgtg tctggaacgc cgttggaga //