| 出品公司: | -- |
|---|---|
| 载体名称: | pmGeos-M-C1 |
| 质粒类型: | 哺乳动物细胞载体；荧光蛋白报告载体 |
| 复制子: | pUC |
| 终止子: | SV40 poly(A) signal |
| 启动子: | CMV |
| 载体大小: | 4692 bp |
| 5' 测序引物及序列: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 哺乳细胞 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pmGeos-M-C1载体质粒基本信息
pmGeos-M-C1质粒图谱载体图谱和pmGeos-M-C1载体序列质粒序列多克隆位点信息
pmGeos-M-C1质粒载体简介
pmGeos-M-C1质粒序列载体序列
LOCUS mGeos-M-C1 4692 bp ds-DNA circular SYN 02-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS mGeos-M-C1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4692)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Friday, September 2, 2016 from SnapGene Viewer 3.1.4
http://www.biofeng.com/
FEATURES Location/Qualifiers
source 1..4692
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1290
/codon_start=1
/product="monomeric variant of green-to-red photoswitchable
fluorescent protein EosFP (McKinney et al., 2009)"
/note="mEos2"
/translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
EGGPLPFAFDILTTAFMYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
NARR"
misc_feature 1291..1356
/note="MCS"
/note="multiple cloning site"
polyA_signal 1480..1601
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1608..2063)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2090..2194
/gene="bla"
/note="AmpR promoter"
promoter 2196..2553
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2404..2539
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2588..3382
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3614..3661
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3990..4578
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaaactccg tatggaaggc
661 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
721 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
781 ctgaccactg cattcatgta cggcaacagg gtattcgcca aatatccaga caacatacaa
841 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
901 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
961 aaagttcgat tttatggtac caactttccc gccaatggtc cagttatgca gaagaagacg
1021 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gacgggtgat
1081 attcatatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
1141 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
1201 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
1261 cattctggat tgcctgacaa tgccagacga tccggactca gatctcgagc tcaagcttcg
1321 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata
1381 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
1441 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
1501 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
1561 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
1621 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
1681 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
1741 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
1801 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
1861 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
1921 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
1981 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
2041 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
2101 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
2161 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
2221 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
2281 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
2341 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
2401 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
2461 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
2521 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
2581 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
2641 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
2701 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
2761 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
2821 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
2881 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
2941 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
3001 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
3061 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
3121 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
3181 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
3241 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
3301 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
3361 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
3421 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
3481 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
3541 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
3601 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
3661 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
3721 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
3781 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
3841 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
3901 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
3961 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
4021 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
4081 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
4141 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
4201 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
4261 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
4321 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
4381 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
4441 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
4501 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
4561 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
4621 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
4681 accgccatgc at
//
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