| 质粒名称: | pYC44 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 63903 |
| 存储实验室: | Irene Castano Navarro |
|
载体骨架基本信息 |
|
| 载体名称: | pRS306 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4372 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 酵母表达 |
| 筛选标记: | URA3 ; Nourseothricin |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | FRT::3'UTRcta::NAT::FRT |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pYC44 was a gift from Irene Castano Navarro (Addgene plasmid # 63903 ; http://n2t.net/addgene:63903 ; RRID:Addgene_63903) |
| 参考文献部分: | Expression vectors for C-terminal fusions with fluorescent proteins and epitope tags in Candida glabrata. Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, Patron-Soberano A, De Las Penas A, Castano I. Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 10.1016/j.fgb.2015.04.020. 10.1016/j.fgb.2015.04.020 PubMed 25986172 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pYC44 5913 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Initial Integrative Vector
.
ACCESSION .
VERSION .
KEYWORDS pYC44
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5913)
AUTHORS Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G,
Patron-Soberano A, De Las Penas A, Castano I
TITLE Expression vectors for C-terminal fusions with fluorescent proteins
and epitope tags in Candida glabrata.
JOURNAL Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi:
10.1016/j.fgb.2015.04.020.
PUBMED 25986172
REFERENCE 2 (bases 1 to 5913)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5913
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(29..51)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 151..170
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 195..410
/gene="S. cerevisiae URA3"
/label=URA3 promoter
CDS 411..1214
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
rep_origin complement(1343..1798)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1480..1501)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1692..1711
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1928..1950
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 1942..1959
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 1943..1959
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1966..1985
/label=T7
/note="T7 promoter, forward primer"
promoter 1966..1984
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 2017..2033
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2017..2033
/label=pBluescriptSK
/note="For pBluescript vector"
protein_bind complement(2035..2068)
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
promoter 2398..2741
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
terminator 3344..3541
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
protein_bind complement(3584..3617)
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
promoter complement(3653..3671)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(3692..3708)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3692..3708)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3705..3727)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3716..3732
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3740..3770)
/label=lac promoter
/note="promoter for the E. coli lac operon"
primer_bind complement(3923..3940)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4094..4682)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4853..5713)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5476..5495
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5714..5818)
/gene="bla"
/label=AmpR promoter
primer_bind 5886..5904
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca
1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atccgaagtt
2041 cctatacttt ctagagaata ggaacttcgt gcgcttttga accacgtaaa gtgctgtaaa
2101 ccagctctat tcaatgaggt aaatgataaa gtttataaga gaccaaaaaa ttcaaaaaaa
2161 aaattgaaaa attatattgg tatttatatg aaacaatagt tcagttcaaa gcgctagctt
2221 ttctcatctc aactaaccgg tattaggtgg gtatttattt aatatgggaa caagaaatgt
2281 gacattaatg ttcatgtttt tattttcatt atttattcta tatggtttgg gacttgatgt
2341 tatgtcttaa ttcatagaga tccgcttgcc tcgtccccgc cgggtcaccc ggccagcgac
2401 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt
2461 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat
2521 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag
2581 ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat
2641 ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg
2701 ctaggataca gttctcacat cacatccgaa cataaacaac cggatcatcc tacaattatg
2761 tctactactt tggatgatac tgcttataga tacagaactt ctgttccagg tgatgctgaa
2821 gctattgaag ctttggatgg ttctttcact accgatactg tttttagagt tactgctact
2881 ggtgatggtt tcactttgag agaagttcca gttgatccac cattgactaa ggttttccca
2941 gatgatgaat ccgatgatga atccgatgct ggtgaagatg gtgatccaga ttctagaact
3001 ttcgttgctt atggtgatga tggtgatttg gctggtttcg ttgttgtttc ttattctggt
3061 tggaacagaa gattgactgt tgaagatatt gaagttgctc cagaacatag aggtcatggt
3121 gttggtagag ctttgatggg tttggctact gaattcgcca gagaaagagg tgctggtcat
3181 ttgtggttgg aagttaccaa tgttaatgct ccagctattc atgcttatag aagaatgggt
3241 ttcactttgt gtggtttgga tactgcttta tacgatggta ctgcttccga tggtgaacaa
3301 gctttgtata tgtctatgcc atgtccataa taactcgacc agtactgaca ataaaaagat
3361 tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta
3421 atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa
3481 gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact
3541 gctgtcgatt cgatactaac gccgccatcc agtgtcgatc gacgaagttc ctatactttc
3601 tagagaatag gaacttcgtc ctcgaggggg ggcccggtac cagcttttgt tccctttagt
3661 gagggttaat tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
3721 atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa gcctggggtg
3781 cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
3841 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
3901 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
3961 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
4021 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
4081 cgttgctggc gtttttccat aggctcggcc cccctgacga gcatcacaaa aatcgacgct
4141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttc ccccctggaa
4201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
4261 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
4321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
4381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
4441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
4501 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
4561 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
4621 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
4681 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
4741 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
4801 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
4861 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
4921 gactgcccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
4981 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
5041 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
5101 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
5161 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
5221 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgaaaaaaa gcggttagct
5281 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
5341 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
5401 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
5461 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
5521 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
5581 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
5641 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
5701 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
5761 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
5821 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
5881 ataaaaatag gcgtatcacg aggccctttc gtc
//
