pGEX-3X

价格:800元

联系方式:I47-825O-882O

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pGEX3X载体基本信息

出品公司: GE
别名: pGEX-3X, pGEX3X
质粒类型: 大肠杆菌蛋白表达载体
表达水平: 高拷贝
启动子: Tac
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4952 bp
5' 测序引物及序列: pGEX5':  GGGCTGGCAAGCCACGTTTGGTG
3' 测序引物及序列: pGEX3': CCGGGAGCTGCATGTGTCAGAGG
载体标签: N-GST
载体抗性: Ampicillin 氨苄
备注:
复制子是pMB1
产品目录号: 27-4803-01
稳定性: 瞬时表达 Transient
组成型: 诱导表达
病毒/非病毒: 非病毒

pGEX3X载体质粒图谱和多克隆位点信息

pGEX-3X载体图谱和多克隆位点

pGEX3X载体简介

pGEX3X载体序列

LOCUS       pGEX-3X	4952 bp 	DNA    circular 	SYN
DEFINITION  pGEX-3X
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="pGEX-3X"
                     /mol_type="other DNA"
     promoter        184..212
                     /label="tac_promoter"
     misc_feature    224..246
                     /label="M13_pUC_rev_primer"
     gene            258..960
                     /label="GST (variant)"
                     /gene="GST (variant)"
     CDS             258..956
                     /label="ORF frame 3"
     misc_feature    complement(1002..1024)
                     /label="pGEX_3_primer"
     promoter        1290..1318
                     /label="AmpR_promoter"
     gene            1360..2220
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             1360..2220
                     /label="ORF frame 1"
     rep_origin      2375..2994
                     /label="pBR322_origin"
     misc_feature    3292..4383
                     /label="lacI"
     CDS             3424..4383
                     /label="ORF frame 1"
     promoter        4432..4461
                     /label="lac_promoter"
     misc_feature    4475..4497
                     /label="M13_pUC_rev_primer"
     promoter        4496..4514
                     /label="M13_reverse_primer"
     CDS             4506..81
                     /label="ORF frame 3"
     misc_feature    4523..4678
                     /label="lacZ_a"
     promoter        complement(4526..4542)
                     /label="M13_forward20_primer"
     misc_feature    complement(4535..4557)
                     /label="M13_pUC_fwd_primer"
ORIGIN
    1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
   61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
  121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
  181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
  241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
  301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
  361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
  421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
  481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
  541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
  601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
  661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
  721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
  781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
  841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
  901 atcctccaaa atcggatctg atcgaaggtc gtgggatccc cgggaattca tcgtgactga
  961 ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
 1021 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
 1081 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
 1141 ggagtgtata attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
 1201 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
 1261 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
 1321 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
 1381 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
 1441 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
 1501 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
 1561 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
 1621 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
 1681 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
 1741 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
 1801 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
 1861 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
 1921 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
 1981 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
 2041 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
 2101 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
 2161 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
 2221 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
 2281 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
 2341 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
 2401 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
 2461 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
 2521 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
 2581 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
 2641 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
 2701 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
 2761 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
 2821 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
 2881 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
 2941 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
 3001 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
 3061 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
 3121 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
 3181 ctccttacgc atctgtgcgg tatttcacac cgcataaatt ccgacaccat cgaatggtgc
 3241 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
 3301 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
 3361 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
 3421 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
 3481 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
 3541 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
 3601 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
 3661 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
 3721 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
 3781 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
 3841 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
 3901 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
 3961 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
 4021 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
 4081 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
 4141 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
 4201 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
 4261 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
 4321 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
 4381 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
 4441 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
 4501 cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
 4561 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
 4621 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
 4681 ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc
 4741 ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc
 4801 ccatctacac caacgtaacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga
 4861 atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc
 4921 agacgcgaat tatttttgat ggcgttggaa tt
//

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