LOCUS pDD103 4743 bp DNA circular SYN 25-AUG-2024
DEFINITION Constitutive expression of mCherry on pAL5000/pMB1 backbone with
kanamycin resistance..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4743)
AUTHORS DeLorenzo DM, Henson WR, Moon TS
TITLE Development of Chemical and Metabolite Sensors for Rhodococcus
opacus PD630.
JOURNAL ACS Synth Biol. 2017 Oct 20;6(10):1973-1978. doi:
10.1021/acssynbio.7b00192. Epub 2017 Aug 10.
PUBMED 28745867
REFERENCE 2 (bases 1 to 4743)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1021/acssynbio.7b00192"; journalName: "ACS Synth Biol"; date:
"2017-10-20- 20"; volume: "6"; issue: "10"; pages: "1973-1978"
FEATURES Location/Qualifiers
source 1..4743
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 1..711
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(146..164)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 414..433
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind complement(738..755)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(909..1497)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(989..1008)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1781..2575)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(1892..1911)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 2502..2521
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
terminator 4448..4475
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
ORIGIN
1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
421 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta acctgaattc
721 gagctggatc cagcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
781 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
841 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
1021 ctcgtgcgct ctcctgtttc gaccctgccg cttaccggat acctgtccgc ctttctccct
1081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
1141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
1201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
1261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
1321 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
1381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
1441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
1501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
1561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttggg gtgggcgaag
1621 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
1681 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
1741 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
1801 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
1861 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
1921 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
1981 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
2041 gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
2101 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
2161 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
2221 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
2281 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
2341 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttggagtt
2401 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
2461 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
2521 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
2581 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg
2641 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
2701 ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa
2761 gcccactgca agctacctgc tttctctttg cgcttgcgtc tgacattcat ccggggtcag
2821 caccgtttct gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct
2881 gagtgcttgc ggcagcgtga agctagcgtc taccaggact tttacctgtc cgacccgttg
2941 caacggaacc ccccacggaa cccccgcgac acccgctccc caattgcgtt agaacagcgg
3001 tggattgtcg gcttcgttgt gggccttttg agccgcttcc tgttctgccg cacgctcttt
3061 cctcgcccga tagccgagtc gcttaacggt gtccagatgc agcccgaaat gtttggccgt
3121 ttgcggccaa gagtggccct cgtcgtcgtg ataggcgcgg atgcgttcgc ggcgtgcagc
3181 ctgctcggcg agccactcgc tgcgttcctg cgccacgagc cggacgacgt ggcgttcgga
3241 tagtccggtg attcgagcgc cttcggcggc ggtcacgcgc cgctttttgc ggacagtcgg
3301 ctgccggttg tagccgtcgc tgtagccgtc gctcatagca atgcctccat ggctgacgcg
3361 gactttgcgc gccgcgcaac tgtgctcgcc gccgtgcgcg ctgctgcgcc cttccgcgag
3421 atggccgact ggcgcgcact gagtgtggcc tcgtagacca cgatcccgtc cgcccaaatg
3481 cgcgacttgg ttgtgatcca acgccaaatg ctgttggcga tggcgcggac ctcgctgtcc
3541 ggtagcggtc cgggacacac gtcgttgcac gggaactcgg cgtttcgcgc gtggcactcg
3601 gcatagatcg cgcggccgag tccgtccacg ttccgggtcg gcaggtagat ccgcatgagg
3661 gcgggacgat aggcccacaa cctgacggaa tcgaacagtg cgcaattccg ccctagcggc
3721 gtcggagccg ctttgtacgt ggtctgctga cgccagcgcg gcggtggcat gttcgcgccg
3781 agctcggcct cgatgtggct gagtgtgtag agatctgagt ggagccattc cgtttcccag
3841 gcgatgtggc cggggttttt ggtcatgagg cctgagtaac tgcggtcgcc gtccacggcg
3901 cgccgaaggc cttcggcgca cgccgccatg tatgcgagcg gcttacgccg cgcgtattcg
3961 gtgcgtggaa caggggcgtt gagtgcccac actgcgtgtg cgtggccgtt ggcgcgattg
4021 cccacgatcg cgttgggcag cggatgggac ccccgggcgc tgagcgctcg gagcgctgcg
4081 tctggatggt ctacgtccac gaccagcagg tttgccagcg ctgttgggtt cgcctcgatg
4141 taccggcggc ctagggccga cgcgcggctt tggcggtaga tcccctcgag cagatcgtcg
4201 cttgccagcg gccagtacgg cagccagagc tgctcaaatt cgtcggcgac gtggctcacg
4261 cttggtagta gaccacgatt aatcaccggt gtatggtccg acacgagctc caagtcagat
4321 atttcgctga ggggccaccc cacaactgca cactcccccg ctctcccgtc gagccctggt
4381 ggtggaacac cagcgacagc cgagcacccc caaccacctg taccaaccac tagttgatta
4441 gctaagcaga aggccatcct gacggatggc ctttttgcgt ttaatactgt ttaaactcta
4501 gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc ggcacgatcc gcgacgtgat
4561 gatcggccgc atccccacgg tgctgcgcag tgagctctac gccatcccgg cgttgatctg
4621 tgcgttcgct gtgcgggctc taacacgtcc tagtatggta ggatgagcaa catttcgacg
4681 ccgagagatt cgccgcccga aatgggcacg atccgcatgc ttaattaaga aggagatata
4741 cat
//
