| 质粒名称: | GBX |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 64123 |
| 存储实验室: | Linzhao Cheng |
|
载体骨架基本信息 |
|
| 载体名称: | pCEP4 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 9809 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | Top10 |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | GFP-2A-BCL2L1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1485 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | SFFV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AsiSI(破坏) |
| 3’克隆位点: | PmeI (破坏) |
| 5’测序引物 | 5-AGGCATGGAAAAATACCAAA, or 5-TAACCAATCAGCCTGCTTCT |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | GBX was a gift from Linzhao Cheng (Addgene plasmid # 64123 ; http://n2t.net/addgene:64123 ; RRID:Addgene_64123) |
| 参考文献部分: | A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Chou BK, Gu H, Gao Y, Dowey SN, Wang Y, Shi J, Li Y, Ye Z, Cheng T, Cheng L. Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214. 10.5966/sctm.2014-0214 PubMed 25742692 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS GBX 9809 bp ds-DNA circular SYN 03-NOV-2022
DEFINITION a modified episomal (EBNA1/OriP) vector expressing human eGFP and
BCL-xL genes.
ACCESSION .
VERSION .
KEYWORDS GBX
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9809)
AUTHORS Chou BK, Gu H, Gao Y, Dowey SN, Wang Y, Shi J, Li Y, Ye Z, Cheng T,
Cheng L
TITLE A Facile Method to Establish Human Induced Pluripotent Stem Cells
From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture
Conditions: A Clinically Compliant Approach.
JOURNAL Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214.
PUBMED 25742692
REFERENCE 2 (bases 1 to 9809)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 3, 2022 from SnapGene Server 1.1.58
http://www.biofrng.com
FEATURES Location/Qualifiers
source 1..9809
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..408
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
CDS 427..1143
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 427..1143
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(472..493)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(733..752)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1080..1101
/label=EGFP-C
/note="EGFP, forward primer"
CDS 1153..1209
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
primer_bind complement(1981..2001)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2399..2410)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2507..2641
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2531..2550)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2585..2604
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2784..2806)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 3366..5155
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
CDS complement(5457..7382)
/codon_start=1
/product="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/label=EBNA1
/note="crucial for latent viral infection, and for episomal
amplification of vectors containing the oriP origin (Okita
et al., 2013)"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGG?GG?GG?G?GGG?G?GGG?GG?GG?G?GGG?G?GGGGGG?GGGGG?
GG?GG?G?GGGAG?GGG?GG?G?GGG?GG?GG?G?GGG?G?GG?GGAGG?G?GG?G?GG
G?GG?GG?G?GGAG?GG?G?GGAGAGGAGGAGAGG?GG?G?GG?GG?G?GGG?GG?GAG
GG?GG?GAGG?GG?G?GG?GGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
GEEGQE"
primer_bind complement(7817..7835)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7903..8007
/gene="bla"
/label=AmpR promoter
CDS 8008..8868
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8226..8245)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9039..9627
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9528..9547
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccgggta ccgcgctagc
421 gccagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
481 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
541 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
601 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
661 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
721 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
781 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
841 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
901 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
961 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
1021 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
1081 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
1141 aagggatccg gagccacgaa cttctctctg ttaaagcaag caggagacgt ggaagaaaac
1201 cccggtccta tgtctcagag caaccgggag ctggtggttg actttctctc ctacaagctt
1261 tcccagaaag gatacagctg gagtcagttt agtgatgtgg aagagaacag gactgaggcc
1321 ccagaaggga ctgaatcgga gatggagacc cccagtgcca tcaatggcaa cccatcctgg
1381 cacctggcag acagccccgc ggtgaatgga gccactggcc acagcagcag tttggatgcc
1441 cgggaggtga tccccatggc agcagtaaag caagcgctga gggaggcagg cgacgagttt
1501 gaactgcggt accggcgggc attcagtgac ctgacatccc agctccacat caccccaggg
1561 acagcatatc agagctttga acaggtagtg aatgaactct tccgggatgg ggtaaactgg
1621 ggtcgcattg tggccttttt ctccttcggc ggggcactgt gcgtggaaag cgtagacaag
1681 gagatgcagg tattggtgag tcggatcgca gcttggatgg ccacttacct gaatgaccac
1741 ctagagcctt ggatccagga gaacggcggc tgggatactt ttgtggaact ctatgggaac
1801 aatgcagcag ccgagagccg aaagggccag gaacgcttca accgctggtt cctgacgggc
1861 atgactgtgg ccggcgtggt tctgctgggc tcactcttca gtcggaaatg agtttaaacg
1921 cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
1981 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
2041 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
2101 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
2161 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
2221 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
2281 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
2341 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
2401 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
2461 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggcggatc cagacatgat
2521 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
2581 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
2641 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
2701 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg
2761 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
2821 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
2881 gggtgtcggg gcgcagccat gaggtcgact ctagaggatc gatccccgcc ccggacgaac
2941 taaacctgac tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag
3001 ttggttggta caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa
3061 cacaaagggg ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa
3121 cactgagtgg ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg
3181 cctttatgtg taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc
3241 aggggcccag gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct
3301 accgataagc ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa
3361 ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa
3421 ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg
3481 gtcctaagga acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg
3541 tcaggattcc acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag
3601 gagcgggcag tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta
3661 atagaataac tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt
3721 tcaggtgacg cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg
3781 acaccaatat aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat
3841 tctgaatatc tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt
3901 ccatctcaca cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg
3961 ggttattaag atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta
4021 tttgtaacaa ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta
4081 acacccccga aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg
4141 ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg
4201 cggttttgga ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac
4261 tgcggtcaaa ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag
4321 taggtgggcg ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca
4381 cttgcgcctg agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag
4441 cacggtgggc taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg
4501 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
4561 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg
4621 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
4681 ctatcctaat ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg
4741 ctatcctaat ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat
4801 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag cataggctat
4861 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
4921 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
4981 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
5041 cctcatgcat atacagtcag catatgatac ccagtagtag agtgggagtg ctatcctttg
5101 catatgccgc cacctcccaa gggggcgtga attttcgctg cttgtccttt tcctgctggt
5161 tgctcccatt cttaggtgaa tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc
5221 tcaccaggta aatgtcgcta atgttttcca acgcgagaag gtgttgagcg cggagctgag
5281 tgacgtgaca acatgggtat gcccaattgc cccatgttgg gaggacgaaa atggtgacaa
5341 gacagatggc cagaaataca ccaacagcac gcatgatgtc tactggggat ttattcttta
5401 gtgcggggga atacacggct tttaatacga ttgagggcgt ctcctaacaa gttacatcac
5461 tcctgccctt cctcaccctc atctccatca cctccttcat ctccgtcatc tccgtcatca
5521 ccctccgcgg cagccccttc caccataggt ggaaaccagg gaggcaaatc tactccatcg
5581 tcaaagctgc acacagtcac cctgatattg caggtaggag cgggctttgt cataacaagg
5641 tccttaatcg catccttcaa aacctcagca aatatatgag tttgtaaaaa gaccatgaaa
5701 taacagacaa tggactccct tagcgggcca ggttgtgggc cgggtccagg ggccattcca
5761 aaggggagac gactcaatgg tgtaagacga cattgtggaa tagcaagggc agttcctcgc
5821 cttaggttgt aaagggaggt cttactacct ccatatacga acacaccggc gacccaagtt
5881 ccttcgtcgg tagtcctttc tacgtgactc ctagccagga gagctcttaa accttctgca
5941 atgttctcaa atttcgggtt ggaacctcct tgaccacgat gctttccaaa ccaccctcct
6001 tttttgcgcc tgcctccatc accctgaccc cggggtccag tgcttgggcc ttctcctggg
6061 tcatctgcgg ggccctgctc tatcgctccc gggggcacgt caggctcacc atctgggcca
6121 ccttcttggt ggtattcaaa ataatcggct tcccctacag ggtggaaaaa tggccttcta
6181 cctggagggg gcctgcgcgg tggagacccg gatgatgatg actgactact gggactcctg
6241 ggcctctttt ctccacgtcc acgacctctc cccctggctc tttcacgact tccccccctg
6301 gctctttcac gtcctctacc ccggcggcct ccactacctc ctcgaccccg gcctccacta
6361 cctcctcgac cccggcctcc actgcctcct cgaccccggc ctccacctcc tgctcctgcc
6421 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
6481 cctscccctc ctsctcctsc ccctcctscc cctcctgctc ctscccctcc tscccctcct
6541 cctgctccts cccctcctsc ccctcctcct sctcctsccc ctcctscccc tcctsctcct
6601 scccctccts cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctsct
6661 cctscccctc ctsctcctgc ccctcctsct cctscccctc ctscccctcc tscccctcct
6721 cctsctccts cccctcctsc tcctscccct cctgcccctc ctscccctcc tsctcctscc
6781 cctcctccts ctcctscccc tcctscccct cctscccctc ctcctsctcc tscccctcct
6841 scccctcctc ctsctcctgc ccctcctcct sctcctsccc ctcctscccc tcctscccct
6901 cctcctcctc ctscccctcc tccccctcct cctsctccts cccctcctcc tsctcctscc
6961 cctcctsccc ctcctscccc tcctcctsct cctscccctc ctcctsctcc tscccctcct
7021 scccctccts cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct
7081 cctgctcctg cccctcccgc tcctgctcct gctcctgttc caccgtgggt ccctttgcag
7141 ccaatgcaac ttggacgttt ttggggtctc cggacaccat ctctatgtct tggccctgat
7201 cctgagccgc ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct
7261 cgtccatggt tatcaccccc tcttctttga ggtccactgc cgccggagcc ttctggtcca
7321 gatgtgtctc ccttctctcc taggccattt ccaggtcctg tacctggccc ctcgtcagac
7381 atgattcaca ctaaaagaga tcaatagaca tctttattag acgacgctca gtgaatacag
7441 ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc tcatcccctt catggtcgct
7501 gtcagacaga tccaggtctg aaaattcccc atcctccgaa ccatcctcgt cctcatcacc
7561 aattactcgc agcccggaaa actcccgctg aacatcctca agatttgcgt cctgagcctc
7621 aagccaggcc tcaaattcct cgtccccctt tttgctggac ggtagggatg gggattctcg
7681 ggacccctcc tcttcctctt caaggtcacc agacagagat gctactgggg caacggaaga
7741 aaagctgggt gcggcctgtg aggatcagct tatcgatgat aagctgtcaa acatgagaat
7801 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
7861 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
7921 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
7981 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
8041 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
8101 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
8161 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
8221 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
8281 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
8341 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
8401 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
8461 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
8521 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
8581 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
8641 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
8701 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
8761 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
8821 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
8881 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
8941 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
9001 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
9061 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
9121 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
9181 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
9241 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
9301 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
9361 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
9421 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
9481 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
9541 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
9601 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
9661 gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg
9721 gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc
9781 atccagcctc gcgtcgcgag ctagctgca
//
