质粒名称: | AND-gate receiver plasmid |
---|---|
出品公司: | Addgene |
目录编号: | 127676 |
存储实验室: | Friedrich Simmel |
载体骨架基本信息 |
|
载体名称: | pSB1A3 |
载体出品公司: | -- |
空载体大小: | 2100 bp |
完整质粒大小: | 3964 bp |
载体修饰: | -- |
载体类型: | 细菌表达,合成生物学 |
筛选标记: | -- |
载体抗性: | 氨苄青霉素,100μg/mL |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 高拷贝 |
插入基因信息 |
|
插入基因名称: | LuxR, GFP |
物种来源: | Synthetic; Aliivibrio fischeri |
插入大小: | 1900 bp |
基因突变: | pLlacO-1 for LuxR, pLux for GFP |
GenBank编号 | -- |
启动子: | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5’克隆位点: | EcoRI(未破坏) |
3’克隆位点: | SpeI (未破坏) |
5’测序引物 | TGCCACCTGACGTCTAAGAA |
3’测序引物 | ATTACCGCCTTTGAGTGAGC |
文献引用方法 |
|
材料和方法部分: | "AND-gate" receiver plasmid was a gift from Friedrich Simmel (Addgene plasmid # 127676 ; http://n2t.net/addgene:127676 ; RRID:Addgene_127676) |
参考文献部分: | Chemical communication between bacteria and cell-free gene expression systems within linear chains of emulsion droplets. Schwarz-Schilling M, Aufinger L, Muckl A, Simmel FC. Integr Biol (Camb). 2016 Apr 18;8(4):564-70. doi: 10.1039/c5ib00301f. Epub 2016 Jan 18. 10.1039/c5ib00301f PubMed 26778746 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS AND-gate receiver plasmid 4028 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Encodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.. ACCESSION . VERSION . KEYWORDS ""AND-gate"" receiver plasmid SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4028) AUTHORS Schwarz-Schilling M, Aufinger L, Muckl A, Simmel FC TITLE Chemical communication between bacteria and cell-free gene expression systems within linear chains of emulsion droplets. JOURNAL Integr Biol (Camb). 2016 Apr 18;8(4):564-70. doi: 10.1039/c5ib00301f. Epub 2016 Jan 18. PUBMED 26778746 REFERENCE 2 (bases 1 to 4028) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..4028 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 6..20 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 27..43 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 64..75 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" terminator 879..950 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 966..993 /label=T7Te terminator /note="phage T7 early transcription terminator" CDS 1090..1806 /codon_start=1 /product="Aequoria victoria green fluorescent protein" /label=GFP /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" primer_bind complement(1120..1148) /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" primer_bind 1743..1763 /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" terminator 1826..1897 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1913..1940 /label=T7Te terminator /note="phage T7 early transcription terminator" misc_feature 1947..1967 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(1973..1990) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2144..2732) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2224..2243) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2903..3763) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3526..3545 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3764..3868) /gene="bla" /label=AmpR promoter primer_bind 3936..3954 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 3958..4001 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 4004..4025 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 ataaatgtga gcggataaca ttgacattgt gagcggataa caagatactg agcactacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag tcacacagga 1081 aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa 1141 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca 1201 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg 1261 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat 1321 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact 1381 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat 1441 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt 1501 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa 1561 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa 1621 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac 1681 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac 1741 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac 1801 aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1861 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1921 ttcgggtggg cctttctgcg tttatatact agtagcggcc gctgcaggct tcctcgctca 1981 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 2041 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 2101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc 2161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 2221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 2281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 2341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 2401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 2461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 2521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 2581 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 2641 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 2701 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 2761 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 2821 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 2881 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 2941 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 3001 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 3061 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 3121 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 3181 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 3241 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 3301 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 3361 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 3421 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 3481 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 3541 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 3601 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 3661 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 3721 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 3781 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 3841 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 3901 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt 3961 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg cggccgcttc 4021 tagagctg //
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
pRK5
2023-07-26pCMV-Gaussia Luc
2023-03-13pCMV-Gluc
2023-04-13pGL4.54[luc2-TK]
2022-10-01pTol2-Promoter-T2A-EGFP
2022-10-01pTol2-appb-APPsw-CMV-EGFP
2022-10-01pGL4.36[luc2P MMTV Hygro]
2022-10-01pGL4.21[luc2P/Puro]
2022-10-01pHBLV-U6-shRNA-ZsGreen-Puro
2022-10-01pRetroX-Tight-Puro-Gluc
2022-10-01
- 上一篇:GBX
- 下一篇:Sender plasmid