P445-FBP_17

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: P445-FBP_17 
出品公司: 爱迪基因
目录编号: 139708
存储实验室: Daniel Voytas

载体骨架基本信息
载体名称: pTRANS_220d
载体出品公司: --
载体抗性: 卡那霉素
空载体大小: --
完整质粒大小: --
载体修饰: --
载体类型: 植物表达,荧光素酶
筛选标记: --
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs
物种来源: Neonothopanus nambi and Aspergillus nidulans
插入大小: --
基因突变: --
GenBank编号 --
启动子 various
标签/融合蛋白: --
克隆方法: 限制性内切酶
5' 克隆位点: AarI(克隆时销毁)
3' 克隆位点: AarI(克隆时销毁)
5' 测序引物 --
3' 测序引物 --

文献引用方法
材料和方法部分: P445-FBP_17 was a gift from Daniel Voytas (Addgene plasmid # 139708 ; http://n2t.net/addgene:139708 ; RRID:Addgene_139708)
参考文献部分: Building customizable auto-luminescent luciferase-based reporters in plants. Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson R, Rizvi F, Imaizumi T, Voytas DF. Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786. 10.7554/eLife.52786 PubMed 32209230

载体图谱质粒图谱和多克隆位点信息

P445-FBP_17质粒图谱

载体简介

载体序列

LOCUS       P445-FBP_17             23318 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  T-DNA encoding Fungal Bioluminescent Pathway Components, complete 
            pathway, Luz promoter is flower specific, circadian controlled.
ACCESSION   .
VERSION     .
KEYWORDS    P445-FBP_17
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23318)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 23318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..23318
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(5..22)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5..21)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..36)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    224..248
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1549..2178
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2607..3680
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3746..3940
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4176..4198)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4284..4424
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4339..4358
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4439..4456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4610..5198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4690..4709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5285..6079)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6504..6528
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6606..6780
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6837..7634)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6948..6967)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7558..7577
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7697..8373)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8564..8585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8600..8630
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8638..8654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8643..8665
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8662..8678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8662..8678
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      12555..12755
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        12792..13172
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     terminator      14444..14696
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     promoter        14808..15153
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    15172..15227
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     polyA_signal    20360..20534
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     terminator      22590..23297
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
       61 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      121 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
      181 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
      241 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
      301 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
      361 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
      421 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
      481 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
      541 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
      601 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
      661 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
      721 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
      781 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
      841 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
      901 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
      961 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     1021 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     1081 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     1141 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     1201 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     1261 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     1321 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc
     1381 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     1441 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     1501 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     1561 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     1621 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     1681 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     1741 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     1801 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     1861 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     1921 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     1981 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     2041 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     2101 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     2161 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     2221 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     2281 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     2341 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     2401 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     2461 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     2521 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     2581 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     2641 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     2701 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     2761 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     2821 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     2881 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     2941 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     3001 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     3061 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     3121 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     3181 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     3241 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     3301 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     3361 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     3421 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     3481 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     3541 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     3601 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     3661 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     3721 aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     3781 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     3841 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     3901 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     3961 cccaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     4021 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     4081 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     4141 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggaa
     4201 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4261 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     4321 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4381 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggccctct tccgcttcct
     4441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5041 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5221 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
     5281 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
     5341 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
     5401 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
     5461 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
     5521 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
     5581 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
     5641 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
     5701 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
     5761 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
     5821 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
     5881 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
     5941 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
     6001 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
     6061 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
     6121 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
     6181 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
     6241 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
     6301 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
     6361 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
     6421 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
     6481 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
     6541 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
     6601 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
     6661 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
     6721 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
     6781 ctcgagcttg tcgatcgact ctagctagag gatcgatccg aaccccagag tcccgctcag
     6841 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     6901 taaagcacga ggaagcggtc agcccattcg ccgccaagtt cttcagcaat atcacgggta
     6961 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     7021 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg tgtcacgacg
     7081 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     7141 ccctgatgtt cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     7201 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     7261 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     7321 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     7381 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     7441 gcctcgtcct ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     7501 cgcccctgcg ctgacagccg aaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     7561 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     7621 tgttcaatcc ccatggtcga tcgacagatc tgcgaaagct cgagagagat agatttgtag
     7681 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
     7741 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
     7801 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     7861 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     7921 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     7981 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     8041 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     8101 gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg
     8161 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
     8221 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
     8281 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
     8341 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
     8401 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct
     8461 agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8521 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8581 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8641 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg
     8701 tacccgggga tcggcgcgcc ctatggagaa tatcattttt aagtaaaatt ttgaattcaa
     8761 atgttacgtg tattatttaa ttcatcaatt tgccttgtca tagcgagtac attacaaaca
     8821 tcacatatat ttgattgatt gtcaaaaaat atcaaaatat atatcaattt taagaggtat
     8881 aggtgtctaa tatgtactag ccctaattta aatatctaaa ttaattattc ggatgaatct
     8941 atataccatc tttttaatgg acacccaaaa tcacacatca aacatcatat acatgttgaa
     9001 aacatattat tgatatagct acatatatgt tttaatataa ataaaagacg agtcatatat
     9061 tcaaaaatta agaatcaaat aattttaatt tatttaatat tcaaaactta atactattta
     9121 aatttagata ttctaatttt aatacacgtc tgataaaata gatgaggact aaataaataa
     9181 tttgagacta tcttttcttt atttggcggc ccacaaataa tttagattct cgtaaccccc
     9241 tctttttctc tcactgaaaa agcacaatcc gtgtccaaac acaaagaagc actcgacacc
     9301 gtagatctcc attcagatca acggcttata ttcagttttc tccattcacg tggatcgaca
     9361 ttcttatccg tccgattatc aataaatttc ccaaaattta gcggccatga ttttaacccc
     9421 gcctcatttc aaaccgccca cgaaatcctc gacgcccaaa ttcaccaact ataaatagcc
     9481 accaccatcc ccttcatcaa tcatcaaatt tcataaccct agaatcatca cctttttcaa
     9541 atttcaatgg ctcctatctc ctccacctgg tccagattaa tcaggttcgt agctatagaa
     9601 acgtcactcg tgcatatagg cgaaccgatt gatgcaacta tggacgtagg gcttgctcgt
     9661 cgagaaggga aaactattca ggcctatgag attatcggat ctggcagcgc cctggacctt
     9721 tctgcacagg tgagtaagaa cgtgctcaca gtacgagaat tgttgatgcc actttctaga
     9781 gaagagataa agacggtcag atgcttaggc cttaactatc ctgtccacgc aacagaagcc
     9841 aatgttgctg taccgaagtt tccgaacctg ttctacaaac cagtcacaag tctcatcggg
     9901 cctgatggtc ttatcacgat ccctagtgta gtgcaaccgc ctaaggaaca tcagtcagat
     9961 tatgaagctg aacttgtgat tgtaatcgga aaggccgcca agaacgtgtc tgaggacgaa
    10021 gccttggatt atgtcttagg ctatacagct gctaacgata ttagtttccg aaaacaccag
    10081 ctcgcagtct cacagtggtc attctcaaaa ggatttggat ctctccttct cactatacgt
    10141 atggcccaga cacatagtgg aaacataaat aggttctcac gtgatcaaat ctttaatgtt
    10201 aagaaaacaa tcagcttttt gtctcaagga actaccctcg aacctgggtc tatcattttg
    10261 actggcaccc ctgatggggt cggcttcgtc aggaacccgc ccttatactt aaaagatggg
    10321 gatgaagtga tgacttggat aggttccggc attggtacgc ttgctaacac cgttcaagaa
    10381 gagaaaacat gctttgcaag cggtgggcat gagtaggctt ttatgttggt gatatggtgg
    10441 taaatgtagg gatttagttt acaattgcgt atgtctgtgt tggatatctg tagtgctgtt
    10501 cttatggctt agatcttgta atttctcatt acagtatcaa tgaatagata tcagtttcta
    10561 gtgatgacat tggttcgtct tttagctgtt gattaatttt tcttaattga ttcatcctat
    10621 tgcaattctt ctgaatttaa attgtatact gtgaaattaa gaaaattctt gaaattaatg
    10681 agaatttgag taatagcgct gttagagctc tgacggcgcg ccctatggag aattcatggg
    10741 gcattacaat taattagtaa tactaatttt aataacacta aatcatatta gtctctatag
    10801 gtataatata taactctgga gtcactatct gtatttggga tcaacagtta caaaggtacg
    10861 agtaaagtct ctgatccgac aaacaatccg tatatcctac cttcccgaaa tttcatgtat
    10921 aaaattatac taaaatatta tttttcttgt attatccgat tgaaaattat gcgaattcaa
    10981 cataaaactc taaaaaaaga aaataatgaa aattacaact taaaagagtg tctaagttgg
    11041 aatcatggta ttacataaag ttgaggacaa tataagcaag caaacaagta ctctagctat
    11101 caatttactt tggactacta tatgataaat atttcaaccc ccctcctccc ccaccaaaaa
    11161 aaaattaaaa taacaagttg aaggactcaa aaaaaaaaaa aaaaaaaact caaaaaaaaa
    11221 aaaaaaaaaa aactcaaaac caacctcaat catacattca tatcctcttc ctacccccat
    11281 cttggatgag ataagattaa cgaggtgctt acacgtgtca cctctattgt ggtgacttaa
    11341 aaaaaattcc aacctttcat atgtagatat taagtaattg tataatgtta tcaagaacca
    11401 cataacatat caaaaacctt atcatttcat tatataaaag gatagtggac atcaaaaggt
    11461 tcatattgaa ccaaaaaaag agagaagaag caaaatggtg caggacactt caagtgctag
    11521 cacaagtcca attttaacca gatggtatat agacactcga cctctcacgg caagtaccgc
    11581 cgcacttccg ctgctggaaa cgctgcaacc agctgatcag atctccgtcc aaaaatatta
    11641 tcatctcaag gacaagcaca tgtcacttgc ctccaacctt ttgaagtatt tattcgttca
    11701 tcgaaattgc cgaataccat ggagtagtat tgtcatatca agaacgcccg acccgcacag
    11761 aaggccttgc tatatacctc cttcaggttc acaagaggac tccttcaaag acggatatac
    11821 gggaatcaac gtagaattca acgttagtca ccaagccagc atggtggcaa tcgcaggtac
    11881 ggctttcaca cctaatagcg gtggagatag caaactcaaa ccggaggtcg gaatagatat
    11941 aacatgtgta aatgagaggc agggcaggaa tggagaagaa agatccttgg aatccctgag
    12001 gcagtatata gatatattca gcgaagtatt cagcaccgca gagatggcta atataaggcg
    12061 acttgatggg gtttcctcaa gttcccttag tgcagacaga ctcgtagatt atggataccg
    12121 attattctac acctattggg ccttaaaaga ggcttatatc aagatgacag gtgaggcctt
    12181 attggctcct tggttgagag agctggagtt ttccaacgtg gtcgctccag ccgctgtcgc
    12241 cgaaagcggg gactctgctg gagactttgg cgaaccgtac actggcgtgc gtacaacatt
    12301 gtataagaac ctcgtagagg acgtacgaat tgaggtagct gctttgggag gagattatct
    12361 gttcgcaaca gccgctagag gtggtggtat aggcgcatcc tcacgtcctg gtggtggccc
    12421 agatggctct ggaattagat cccaggaccc ttggaggccg ttcaagaaac tcgatataga
    12481 gcgtgacata cagccatgtg ctacgggcgt ttgtaattgc ctgtcctcag gataggcttg
    12541 atcccccgtc gacagctagc tatatcatca atttatgtat tacacataat atcgcactca
    12601 gtctttcatc tacggcaatg taccagctga tataatcagt tattgaaata tttctgaatt
    12661 taaacttgca tcaataaatt tatgtttttg cttggactat aatacctgac ttgttatttt
    12721 atcaataaat atttaaacta tatttctttc aagatcgctg ttagagctcg gacggcgcgc
    12781 cctatggaga tttttcaaat cagtgcgcaa gacgtgacgt aagtatccga gtcagttttt
    12841 atttttctac taatttggtc gtttatttcg gcgtgtagga catggcaacc gggcctgaat
    12901 ttcgcgggta ttctgtttct attccaactt tttcttgatc cgcagccatt aacgactttt
    12961 gaatagatac gctgacacgc caagcctcgc tagtcaaaag tgtaccaaac aacgctttac
    13021 agcaagaacg gaatgcgcgt gacgctcgcg gtgacgccat ttcgcctttt cagaaatgga
    13081 taaatagcct tgcttcctat tatatcttcc caaattacca atacattaca ctagcatctg
    13141 aatttcataa ccaatctcga tacaccaaat cgaatggcat cctttgaaaa ttcactcagt
    13201 gtcttaatag ttggggctgg cttaggtggt ttggccgctg ctatagcact gcgaaggcaa
    13261 ggacatgtag tcaagatata cgatagttct tcattcaagg ccgaattggg agcaggatta
    13321 gctgtaccac ccaatactct ccgttccctg caacaactgg gctgtaatac agaaaacctg
    13381 aacggagtag acaacctctg ctttacggcc atgggatacg acgggagtgt aggtatgatg
    13441 aataatatga ccgactaccg tgaggcatac ggcacttcct ggataatggt acatcgagtg
    13501 gatctccaca acgagctgat gcgagtcgcc ttggatccgg gcgggttagg gcctcctgcc
    13561 actcttcacc ttaaccatcg tgttacgttc tgcgatgtgg atgcctgtac agtcacgttt
    13621 acgaacggga cgacacagtc tgctgacttg attgtcggag ctgatggtat taggtctacg
    13681 attcgaagat tcgttctgga ggaggacgtg actgttccag ctagcggaat tgtcggcttt
    13741 cgatggttgg tccaggccga tgctctcgac ccatatcccg aactggattg gattgtcaaa
    13801 aaaccaccct tgggagccag gttgatttct actccacaga acccgcagag tggcgtagga
    13861 ctggccgata gacgaacgat cattatttac gcatgcagag gtgggaccat ggttaatgta
    13921 ttagccgtac acgacgacga aagagatcaa aacactgctg attggtccgt tcccgcatca
    13981 aaggatgatt tattcagagt gtttcacgat taccatccaa gattccgacg actcttagag
    14041 ttagcacagg acattaattt atggcagatg agggtggtcc cggtcttgaa aaaatgggtg
    14101 aacaagaggg tatgcttgct gggtgatgcc gcccatgctt cactgcccac acttggacaa
    14161 ggcttcggga tggggttgga ggatgcagtc gctttgggca cgttgttgcc taagggaacg
    14221 accgcatcac agatcgagac aaggctggcc gtttacgaac agctgcgaaa ggatcgagct
    14281 gagtttgtcg ccgcagagag ctatgaggag caatatgtcc cagagatgag agggctgtac
    14341 cttaggtcca aagaattgag agacagagtg atgggttatg atattaaggt agagtcagag
    14401 aaggttttgg aaacactgtt gcgatcatcc aactcagctt gagcttggac tcccatgttg
    14461 gcaaaggcaa ccaaacaaac aatgaatgat ccgctcctgc atatggggcg gtttgagtat
    14521 ttcaactgcc atttgggctg aattgtagac atgctcctgt cagaaattcc gtgatcttac
    14581 tcaatattca gtaatctcgg ccaatatcct aaatgtgcgt ggctttatct gtctttgtat
    14641 tgtttcatca attcatgtaa cgtttgcttt tcttatgaat tttcaaataa attatccgct
    14701 gttagagctc ccggggcgcg ccctatggag gtcaacatgg tggagcacga cactctggtc
    14761 tactccaaaa atgtcaaaga tacagtctca gaagatcaaa gggctattga gacttttcaa
    14821 caaaggataa tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
    14881 gaaaggacag tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag
    14941 gctatcattc aagatctctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
    15001 agcatcgtgg aaaaagaaga ggttccaacc acgtctacaa agcaagtgga ttgatgtgac
    15061 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
    15121 atataaggaa gttcatttca tttggagagg acacgctact gtatttttac aacaattacc
    15181 aacaacaaca aacaacaaac aacattacaa ttactattta caattacaat gaatagtagt
    15241 aaaaaccctc catccactct gttagatgtt tttctggata ccgctaggaa ccttgataca
    15301 gcatccagaa atgtcctgga gtgcggagaa cacaggtgga gttacagaga gttggacaca
    15361 gtgtcatccg cattggccca acaccttcga tacactgtgg gtcttagccc cactgtagcc
    15421 gtgattagcg agaatcaccc ctacatactt gcattaatgc tcgccgtatg gaaactcggc
    15481 ggaacgttcg caccgataga tgttcattcc ccagccgaac ttgttgctgg aatgttgaat
    15541 attgtaagcc cgtcatgtct tgtcattcct agctctgatg tgacaaatca gacattggca
    15601 tgcgatctta atataccggt tgttgcattc cacccacatc aaagtacaat tcccgaactc
    15661 aacaagaagt atctcacaga ttctcagatc agtcctgacc tgcccttccc cgaccctaat
    15721 cgacctgctt tgtacttatt cacgtcatct gcaacgtcta ggtccaattt gaagtgcgtg
    15781 ccacttacac acacattcat attgcgaaac tccttaagta aacgagcatg gtgcaaaaga
    15841 atgaggccag agacagattt tgatggtatt agggtcctgg ggtgggcccc ctggagccat
    15901 gtcctcgctc atatgcagga cattggccct cttacattat tgaatgccgg gtgctacgta
    15961 tttgccacga ccccatctac ctacccgacg gagctgaagg atgaccgtga tgttattagt
    16021 tgcgccgcaa atgccgtaat gtacaaaggt gttaaatcct tcgcatgcct cccattcgtg
    16081 cttgggggac tcaaggcttt atgtgagtca gagcccagtg ttaaggcaca actgcaagtg
    16141 gaggaacgag cccagctttt gaagtcactc caacacatga aaatcctgga atgcgggggg
    16201 gcaatgctcg aagtgagtgt ggcaagctgg gctatagaga ataggattcc tatatctatt
    16261 gggattggta tgacagagac aggcggcgct cttttcgctg ggccggtcca agcaattcaa
    16321 accggcttta gcagtgaaga taaatttatc gaggacgcaa cctacttgct ggtcaaggac
    16381 gattacgaga gccacgcaga ggaggacata aacgagggcg agttagtggt aaagtccaga
    16441 atgttaccta ggggttattt gggctataac gacccctctt tctctgttga cgatgctggt
    16501 tgggttactt tcaaaactgg agatcgatat tcagtcactc ctgacggcaa attctcctgg
    16561 cttggtcgaa atactgattt cattcagatg acgagtggtg agactctcga tccccgtccg
    16621 attgagtccc tcctttgtga gtccagcctt atatcacgag cctgtgtcat cggggataag
    16681 ttcctgaacg gaccagccac agctgtatgc gctattatag aactcgaacc aacgactgtt
    16741 gagaaaggcc aggctcacag cagggatatt gcccgaatct ttgcccccat caatcgagat
    16801 cttcctcccc cgttacgtat agcatggagt cacgtacttg tacttcagcc aagcgagaaa
    16861 ataccgatga cgaaaaaggg tacaatcttc cgtaagaaaa tcgaacaggt tttcggttcc
    16921 gctttaggag gcagtagcgg cgacaactca caagcaacca ctgacgcttc agtagttagg
    16981 agggatgagt tgagcaacac ggtcaaacat atcataagca gagttttggg agtctccgac
    17041 gatgaactcc tttggacact gagctttgct gagcttggca tgacgtccgc tcttgctact
    17101 agaatagcaa atgaattgaa cgaagttctg gtaggtgtga acctccccat caatgcatgc
    17161 tacattcatg tggacctgcc ttctttgtca aatgccgtgt atgcaaagtt agcacacctt
    17221 aaactcccgg ataggacgcc cgagccaagg aaggccccag ttgaaaatcc cggtggaaag
    17281 gaaatcgtaa tagtaggtca ggcttttagg cttccgggga gtataaatga tgtcgcttct
    17341 ttgagggacg cttttttggc tagacaagca tccagtatta ttactgaaat accgccagat
    17401 agatgggacc acgcatcatt ttatccaaaa gacatacgat ttaataaagc agggctcgtc
    17461 gacatcgcta actacgacca cagttttttc ggattgacag ccacggaggc actgtacctg
    17521 tcaccaacga tgcgtttggc acttgaagtg tcctttgaag ccttggagaa tgctaatata
    17581 cccgtgtccc agttaaaggg ctctcaaaca gctgtatatg tcgcaaccac cgacgacggg
    17641 tttgagacac tgctgaatgc tgaggcagga tatgatgcat acacacgatt ctatggcact
    17701 ggtagggccg cctctacggc cagtggacga attagttatt tactcgacgt acacggccct
    17761 tcaattaccg tagacactgc ttgttcaggc ggtgctgttt gcatagacca ggccatagac
    17821 tacctccaat cctctagcgc agctgatacc gccataatct gcgccagcaa cactcactgc
    17881 tggccaggtt ccttcatgtt tctgagtgct caaggtatgg taagttccgg ggggcgatgt
    17941 gcaactttta cgacggatgc cgacggctat gtaccctctg aaggtgctgt ggcatttatc
    18001 ctcaaaaccc gagaagcagc tatgagagat aaagatacta tcttggcaac tatcaaagca
    18061 acgcagatta gtcataatgg tcgttcccaa ggtttagtcg caccaaacgt caattcacaa
    18121 gctgatttac acagaagctt attgcagaaa gctggtttga gtccagccga cattcacttc
    18181 atcgaagctc acgggacagg aacgtcactc ggtgacctta gtgaaataca agcaattaat
    18241 gatgcctaca cgtcatctca gcctcgaaca gccggaccct taatagtaag cgcaagtaaa
    18301 accgtcattg gtcacacaga gcctgctgga ccgttggtag gcatgctctc agttctcaac
    18361 tcctttaaag agggcgcagt tcccggctta gctcacctga cagcagataa cctcaatccg
    18421 gctttagact gctcctccgt ccctttactt atcccctacc agcctgtcca cttggcagct
    18481 cctaagccac acagggctgc tgtgatgagt tatggcttct ccggcactct tggaggtata
    18541 gttcttgaag caccggatga ggaaaggctt gaggaagaac cacccaacga taagcctatg
    18601 ttatttgttg ttagcgccaa aactcatact gcattgattg agtaccttgg acgatacctt
    18661 gaatttctgc tccaggccaa tccccaagac ttctgcgata tctgttacac tagctgcgtt
    18721 ggacgtgagc attacaggta ccgttttgca tgtgtggcaa acgatatgga agatttaatt
    18781 ggccaacttc agaagaggct tgggtctaag gttccaccta aaccgtccta taaaagagga
    18841 gccctcgctt ttgctttcag tggacaaggg acccagtttc gtggaatggc aacagaattg
    18901 gctaaggcat atagtggttt tcgtaagata gtcagtgatc ttgctaaaag agcatcagaa
    18961 ctgtccggcc atgcaatcga caggtttctg cttgcatatg acattggggc tgagaatgtt
    19021 gcaccggata gtgaagctga tcaaatctgc atattcgttt accaatgtag tgttctcaga
    19081 tggctccaaa caatggggat tcgtccgtca gccgtgatcg gtcacagcct cggtgagatt
    19141 agcgcatccg tagctgctgg agcattgtcc ttggactctg cccttgacct cgtaataagt
    19201 agagccaggt tactgaggtc atctacgaat gccccagctg gcatggcagc tatgtctgca
    19261 tcccaggatg aagtagtcga actcatagga aagctcgatt tagataaagc taatagcctc
    19321 agcgtgtccg taataaacgg cccccagaac accgttgttt caggatcttc tgcagcaata
    19381 gaaagtatcg ttgctctcgc aaagggacga aaaatcaagg ccagcgcact caacattaac
    19441 caggcattcc atagtcctta cgtcgattct gcagtacccg gcttacgagc ttggagtgag
    19501 aaacatatat catcagctcg accattgcaa atccctttgt actctaccct cttgggcgct
    19561 caagtttctg agggtcaaat gttaaatccc gatcactggg tagaccatgc taggaaaccc
    19621 gttcagttcg cacaagcagc cactataatg aaggaatctt tcaccggagt aataatcgat
    19681 attggacctc aagttgtagc atggtccctg ctcttaagca atggtcttac ttccgtgacc
    19741 gcactcgccg caaagcgtgg ccgttcacag caagtggcct tcctctccgc cttagctgat
    19801 ctttatcagg actacggcgt cgtaccagac tttgttggac tgtacgcaca acaggaggac
    19861 gcttccagac tgaaaaagac cgatatctta acttacccct ttcagagggt tcgacgttac
    19921 ccttccttta taccctctag aagggcaccc acccacgctc atgtccaaga cgaggaaact
    19981 ctgtcatcag gatcatcaac tccaacctta gaaaatactg acctggacag tgggaaggaa
    20041 tctctgatgg gaccaacaag agggcttttg cgagtcgatg atcttaggga ctcaatagtc
    20101 agttcagtca aagatgtcct ggaattgaag agtaacgaag atctggacct cagcgagtcc
    20161 cttaatgctc ttggcatgga ctctataatg ttcgctcaat tacgaaaaag gataggggag
    20221 ggcctcggcc tgtccgttcc aatggttttt ctcagcgatg ccttcagtat tggagaaatg
    20281 gtcagtaacc tcgtggaaca ggccgaagct tccgaggaca attgagcttc tctagctaga
    20341 gtcgatcgac aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa
    20401 ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
    20461 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta
    20521 ctaaaatcca gatcgctgtt agagctctcc cggcgcgccc tatggagcag ttctttcaat
    20581 gtaattccgc agtttagggc gtggccttgt atatcaaata attccaaaaa gtacgataaa
    20641 ataggacata aacctaataa aaaatatctt gatacataat atacctctta ctcaatgcag
    20701 ttaggggcgt ggtccttgta tattaattaa ttccaaaatt aagtacgaaa aaaattggac
    20761 ataaacctaa taaaaaatat ctccatacat aatacctctt attcaacctt ctagtgctag
    20821 attttcagtt taattggtaa caaatagtat ctacgttcac atggtttgac aaactcatca
    20881 aagtaaacaa atcaaacgaa tgttttaact aagctgagaa taagatatca tgggtaaaca
    20941 tagtacgaga ttacttccca tgtatgaaag gtcccgtaaa cgtggtgatg acgaaatggt
    21001 tgagtaagcg tgcaatcaca gagacagtag atagtgtatg aaaatgggaa gaaatttaag
    21061 atttaaaaaa aacacaaatc tttggatatg tggggcaaaa ggtttaaaga gcaagaagta
    21121 tagtggaatg tttgaatttt attgacttag gtgtttgggt taacatcatt tctttctttc
    21181 ccaccagtgt ttcaattgat ctgaaattta tgcgaaaaaa tttgcacttt ggatcgtaaa
    21241 acacttccta tatatcaaat acgactccat atcaagagtt tatattttcc aacaacatac
    21301 cgagtatagt cctacaagta ggatctggag agggtgatac gtacaaagac cttaccctca
    21361 ccaaaagtgg agaggctgtt ttcgataaat tctcaactca atgaaagagt tcaaatttta
    21421 gatacctcta attaattatg gaccaatatt tatctctcca cacaatcagc tatcattcac
    21481 tgtcacactt cctttggtgg agtcgataac accagaaaag gaattgtagt tcatgagtag
    21541 ctttcacaaa cccattggtg ggccatggaa tcttacattc tctctcacta tatatatagc
    21601 catacacttc ccattaatca ataaatatat atgtttaaac aaaactttag agagaaagga
    21661 gagaactaat tgcaagaaaa gttggtagat ttttttatat atttaggtgg gtagaagggc
    21721 aataaaatta gcctacacaa tttctttttt gttggtggta gctgagagtc agtagtgaat
    21781 gcgtataaat atctctctgt ctagcttatt cgagagatta agcaaactca gctctcgtag
    21841 catagctatt acatgtggtg tcgtcctcgc ctctgctatt gcttttccca tcatacgacg
    21901 agattatcag acgttcttag aggttggtcc tagctatgcc cctcaaaatt ttcgaggcta
    21961 cattatcgtt tgcgtactca gcttgttccg acaagagcaa aaagggttgg caatctacga
    22021 cagactccca gagaagagaa gatggctggc cgatctccca tttcgtgaag gtactagacc
    22081 tagtataaca tcacacataa tacagagaca gcgaactcaa cttgtagacc aagagtttgc
    22141 cacacgagaa ctgatagaca aagttattcc cagggtgcaa gcacgacata cagataaaac
    22201 atttttgagc acgagcaagt ttgaatttca tgctaaagcc attttcctgt tgccaagtat
    22261 tcctataaac gaccccttaa acatacctag tcatgatacg gttcgtagaa cgaaacgaga
    22321 gattgcacac atgcatgact accacgactg tactcttcac ttagctcttg cagctcaaga
    22381 tggaaaagaa gtcctgaaaa aaggctgggg ccaacgtcat ccgcttgccg ggccgggtgt
    22441 acctgggccg ccgacagaat ggactttctt atacgcaccc cgtaatgagg aagaagctag
    22501 ggtagtagaa atgattgtcg aggcaagtat cggatacatg actaatgatc cggcaggtaa
    22561 aatcgtcgag aatgccaaat gagcttgtcc tgctttaatg agatatgcga gaagcctatg
    22621 atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta
    22681 gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg
    22741 tatttttatg aataatattc tccgttcaat ttactgattg taccctacta cttatatgta
    22801 caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca tctatgatag
    22861 agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta aaaaaagcgg
    22921 cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt caaaagtgcc
    22981 ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac tgaagggaac
    23041 tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt ggccgtccgc
    23101 tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg gtaaccgact
    23161 tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa tgaagtgcag
    23221 gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac
    23281 atgtcgaggc tcagcaggac cgctgttaga gctcagtg
//

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