质粒名称: | P445-FBP_17 |
---|---|
出品公司: | 爱迪基因 |
目录编号: | 139708 |
存储实验室: | Daniel Voytas |
载体骨架基本信息 |
|
载体名称: | pTRANS_220d |
载体出品公司: | -- |
载体抗性: | 卡那霉素 |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 植物表达,荧光素酶 |
筛选标记: | -- |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
|
插入基因名称: | pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs |
物种来源: | Neonothopanus nambi and Aspergillus nidulans |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | various |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | AarI(克隆时销毁) |
3' 克隆位点: | AarI(克隆时销毁) |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
|
材料和方法部分: | P445-FBP_17 was a gift from Daniel Voytas (Addgene plasmid # 139708 ; http://n2t.net/addgene:139708 ; RRID:Addgene_139708) |
参考文献部分: | Building customizable auto-luminescent luciferase-based reporters in plants. Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson R, Rizvi F, Imaizumi T, Voytas DF. Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786. 10.7554/eLife.52786 PubMed 32209230 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS P445-FBP_17 23318 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlled. ACCESSION . VERSION . KEYWORDS P445-FBP_17 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 23318) AUTHORS Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson R, Rizvi F, Imaizumi T, Voytas DF TITLE Building customizable auto-luminescent luciferase-based reporters in plants. JOURNAL Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786. PUBMED 32209230 REFERENCE 2 (bases 1 to 23318) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..23318 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(5..22) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(5..21) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(14..36) /label=M13/pUC Forward /note="In lacZ gene" misc_feature 224..248 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 1549..2178 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 2607..3680 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 3746..3940 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(4176..4198) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 4284..4424 /label=bom /note="basis of mobility region from pBR322" primer_bind 4339..4358 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(4439..4456) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4610..5198) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4690..4709) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5285..6079) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 6504..6528 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" polyA_signal 6606..6780 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(6837..7634) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6948..6967) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 7558..7577 /label=Neo-R /note="Neomycin resistance gene, reverse primer" promoter complement(7697..8373) /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" protein_bind 8564..8585 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8600..8630 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8638..8654 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8643..8665 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 8662..8678 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 8662..8678 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" terminator 12555..12755 /label=gene 7 terminator /note="transcription terminator for octopine-type Ti plasmid gene 7" promoter 12792..13172 /label=MAS promoter /note="mannopine synthase promoter (Velten et al., 1984)" terminator 14444..14696 /label=MAS terminator /note="mannopine synthase terminator" promoter 14808..15153 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" misc_feature 15172..15227 /label=TMV Omega /note="translational enhancer from the tobacco mosaic virus 5'-leader sequence (Gallie et al., 1988)" polyA_signal 20360..20534 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" terminator 22590..23297 /label=OCS terminator /note="octopine synthase terminator" ORIGIN 1 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta 61 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg 121 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg 181 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc 241 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa 301 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc 361 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc 421 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc 481 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg 541 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg 601 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg 661 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg 721 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc 781 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg 841 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt 901 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga 961 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc 1021 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg 1081 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg 1141 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg 1201 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca 1261 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc 1321 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc 1381 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga 1441 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc 1501 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc 1561 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc 1621 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc 1681 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg 1741 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg 1801 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg 1861 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag 1921 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa 1981 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt 2041 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt 2101 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt 2161 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc 2221 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag 2281 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga 2341 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc 2401 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga 2461 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga 2521 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact 2581 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa 2641 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc 2701 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa 2761 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca 2821 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata 2881 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg 2941 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca 3001 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca 3061 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg 3121 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag 3181 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga 3241 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa 3301 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg 3361 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg 3421 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag 3481 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt 3541 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg 3601 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg 3661 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg 3721 aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt 3781 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac 3841 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa 3901 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag 3961 cccaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt 4021 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc 4081 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca 4141 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggaa 4201 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 4261 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg 4321 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 4381 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggccctct tccgcttcct 4441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 4501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 4561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 4621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 4681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 4741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 4801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 4861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 4921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 4981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 5041 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 5101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 5161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 5221 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta 5281 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga 5341 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg 5401 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa 5461 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg 5521 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat 5581 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca 5641 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata 5701 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc 5761 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat 5821 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca 5881 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt 5941 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag 6001 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg 6061 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc 6121 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct 6181 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc 6241 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa 6301 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt 6361 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg 6421 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg 6481 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt 6541 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt 6601 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag 6661 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga 6721 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa 6781 ctcgagcttg tcgatcgact ctagctagag gatcgatccg aaccccagag tcccgctcag 6841 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg 6901 taaagcacga ggaagcggtc agcccattcg ccgccaagtt cttcagcaat atcacgggta 6961 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca 7021 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg tgtcacgacg 7081 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc 7141 ccctgatgtt cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt 7201 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta 7261 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat 7321 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg 7381 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct 7441 gcctcgtcct ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg 7501 cgcccctgcg ctgacagccg aaacacggcg gcatcagagc agccgattgt ctgttgtgcc 7561 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct 7621 tgttcaatcc ccatggtcga tcgacagatc tgcgaaagct cgagagagat agatttgtag 7681 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg 7741 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac 7801 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg 7861 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc 7921 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga 7981 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa 8041 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga 8101 gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg 8161 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca 8221 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct 8281 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg 8341 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta 8401 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct 8461 agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 8521 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 8581 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 8641 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg 8701 tacccgggga tcggcgcgcc ctatggagaa tatcattttt aagtaaaatt ttgaattcaa 8761 atgttacgtg tattatttaa ttcatcaatt tgccttgtca tagcgagtac attacaaaca 8821 tcacatatat ttgattgatt gtcaaaaaat atcaaaatat atatcaattt taagaggtat 8881 aggtgtctaa tatgtactag ccctaattta aatatctaaa ttaattattc ggatgaatct 8941 atataccatc tttttaatgg acacccaaaa tcacacatca aacatcatat acatgttgaa 9001 aacatattat tgatatagct acatatatgt tttaatataa ataaaagacg agtcatatat 9061 tcaaaaatta agaatcaaat aattttaatt tatttaatat tcaaaactta atactattta 9121 aatttagata ttctaatttt aatacacgtc tgataaaata gatgaggact aaataaataa 9181 tttgagacta tcttttcttt atttggcggc ccacaaataa tttagattct cgtaaccccc 9241 tctttttctc tcactgaaaa agcacaatcc gtgtccaaac acaaagaagc actcgacacc 9301 gtagatctcc attcagatca acggcttata ttcagttttc tccattcacg tggatcgaca 9361 ttcttatccg tccgattatc aataaatttc ccaaaattta gcggccatga ttttaacccc 9421 gcctcatttc aaaccgccca cgaaatcctc gacgcccaaa ttcaccaact ataaatagcc 9481 accaccatcc ccttcatcaa tcatcaaatt tcataaccct agaatcatca cctttttcaa 9541 atttcaatgg ctcctatctc ctccacctgg tccagattaa tcaggttcgt agctatagaa 9601 acgtcactcg tgcatatagg cgaaccgatt gatgcaacta tggacgtagg gcttgctcgt 9661 cgagaaggga aaactattca ggcctatgag attatcggat ctggcagcgc cctggacctt 9721 tctgcacagg tgagtaagaa cgtgctcaca gtacgagaat tgttgatgcc actttctaga 9781 gaagagataa agacggtcag atgcttaggc cttaactatc ctgtccacgc aacagaagcc 9841 aatgttgctg taccgaagtt tccgaacctg ttctacaaac cagtcacaag tctcatcggg 9901 cctgatggtc ttatcacgat ccctagtgta gtgcaaccgc ctaaggaaca tcagtcagat 9961 tatgaagctg aacttgtgat tgtaatcgga aaggccgcca agaacgtgtc tgaggacgaa 10021 gccttggatt atgtcttagg ctatacagct gctaacgata ttagtttccg aaaacaccag 10081 ctcgcagtct cacagtggtc attctcaaaa ggatttggat ctctccttct cactatacgt 10141 atggcccaga cacatagtgg aaacataaat aggttctcac gtgatcaaat ctttaatgtt 10201 aagaaaacaa tcagcttttt gtctcaagga actaccctcg aacctgggtc tatcattttg 10261 actggcaccc ctgatggggt cggcttcgtc aggaacccgc ccttatactt aaaagatggg 10321 gatgaagtga tgacttggat aggttccggc attggtacgc ttgctaacac cgttcaagaa 10381 gagaaaacat gctttgcaag cggtgggcat gagtaggctt ttatgttggt gatatggtgg 10441 taaatgtagg gatttagttt acaattgcgt atgtctgtgt tggatatctg tagtgctgtt 10501 cttatggctt agatcttgta atttctcatt acagtatcaa tgaatagata tcagtttcta 10561 gtgatgacat tggttcgtct tttagctgtt gattaatttt tcttaattga ttcatcctat 10621 tgcaattctt ctgaatttaa attgtatact gtgaaattaa gaaaattctt gaaattaatg 10681 agaatttgag taatagcgct gttagagctc tgacggcgcg ccctatggag aattcatggg 10741 gcattacaat taattagtaa tactaatttt aataacacta aatcatatta gtctctatag 10801 gtataatata taactctgga gtcactatct gtatttggga tcaacagtta caaaggtacg 10861 agtaaagtct ctgatccgac aaacaatccg tatatcctac cttcccgaaa tttcatgtat 10921 aaaattatac taaaatatta tttttcttgt attatccgat tgaaaattat gcgaattcaa 10981 cataaaactc taaaaaaaga aaataatgaa aattacaact taaaagagtg tctaagttgg 11041 aatcatggta ttacataaag ttgaggacaa tataagcaag caaacaagta ctctagctat 11101 caatttactt tggactacta tatgataaat atttcaaccc ccctcctccc ccaccaaaaa 11161 aaaattaaaa taacaagttg aaggactcaa aaaaaaaaaa aaaaaaaact caaaaaaaaa 11221 aaaaaaaaaa aactcaaaac caacctcaat catacattca tatcctcttc ctacccccat 11281 cttggatgag ataagattaa cgaggtgctt acacgtgtca cctctattgt ggtgacttaa 11341 aaaaaattcc aacctttcat atgtagatat taagtaattg tataatgtta tcaagaacca 11401 cataacatat caaaaacctt atcatttcat tatataaaag gatagtggac atcaaaaggt 11461 tcatattgaa ccaaaaaaag agagaagaag caaaatggtg caggacactt caagtgctag 11521 cacaagtcca attttaacca gatggtatat agacactcga cctctcacgg caagtaccgc 11581 cgcacttccg ctgctggaaa cgctgcaacc agctgatcag atctccgtcc aaaaatatta 11641 tcatctcaag gacaagcaca tgtcacttgc ctccaacctt ttgaagtatt tattcgttca 11701 tcgaaattgc cgaataccat ggagtagtat tgtcatatca agaacgcccg acccgcacag 11761 aaggccttgc tatatacctc cttcaggttc acaagaggac tccttcaaag acggatatac 11821 gggaatcaac gtagaattca acgttagtca ccaagccagc atggtggcaa tcgcaggtac 11881 ggctttcaca cctaatagcg gtggagatag caaactcaaa ccggaggtcg gaatagatat 11941 aacatgtgta aatgagaggc agggcaggaa tggagaagaa agatccttgg aatccctgag 12001 gcagtatata gatatattca gcgaagtatt cagcaccgca gagatggcta atataaggcg 12061 acttgatggg gtttcctcaa gttcccttag tgcagacaga ctcgtagatt atggataccg 12121 attattctac acctattggg ccttaaaaga ggcttatatc aagatgacag gtgaggcctt 12181 attggctcct tggttgagag agctggagtt ttccaacgtg gtcgctccag ccgctgtcgc 12241 cgaaagcggg gactctgctg gagactttgg cgaaccgtac actggcgtgc gtacaacatt 12301 gtataagaac ctcgtagagg acgtacgaat tgaggtagct gctttgggag gagattatct 12361 gttcgcaaca gccgctagag gtggtggtat aggcgcatcc tcacgtcctg gtggtggccc 12421 agatggctct ggaattagat cccaggaccc ttggaggccg ttcaagaaac tcgatataga 12481 gcgtgacata cagccatgtg ctacgggcgt ttgtaattgc ctgtcctcag gataggcttg 12541 atcccccgtc gacagctagc tatatcatca atttatgtat tacacataat atcgcactca 12601 gtctttcatc tacggcaatg taccagctga tataatcagt tattgaaata tttctgaatt 12661 taaacttgca tcaataaatt tatgtttttg cttggactat aatacctgac ttgttatttt 12721 atcaataaat atttaaacta tatttctttc aagatcgctg ttagagctcg gacggcgcgc 12781 cctatggaga tttttcaaat cagtgcgcaa gacgtgacgt aagtatccga gtcagttttt 12841 atttttctac taatttggtc gtttatttcg gcgtgtagga catggcaacc gggcctgaat 12901 ttcgcgggta ttctgtttct attccaactt tttcttgatc cgcagccatt aacgactttt 12961 gaatagatac gctgacacgc caagcctcgc tagtcaaaag tgtaccaaac aacgctttac 13021 agcaagaacg gaatgcgcgt gacgctcgcg gtgacgccat ttcgcctttt cagaaatgga 13081 taaatagcct tgcttcctat tatatcttcc caaattacca atacattaca ctagcatctg 13141 aatttcataa ccaatctcga tacaccaaat cgaatggcat cctttgaaaa ttcactcagt 13201 gtcttaatag ttggggctgg cttaggtggt ttggccgctg ctatagcact gcgaaggcaa 13261 ggacatgtag tcaagatata cgatagttct tcattcaagg ccgaattggg agcaggatta 13321 gctgtaccac ccaatactct ccgttccctg caacaactgg gctgtaatac agaaaacctg 13381 aacggagtag acaacctctg ctttacggcc atgggatacg acgggagtgt aggtatgatg 13441 aataatatga ccgactaccg tgaggcatac ggcacttcct ggataatggt acatcgagtg 13501 gatctccaca acgagctgat gcgagtcgcc ttggatccgg gcgggttagg gcctcctgcc 13561 actcttcacc ttaaccatcg tgttacgttc tgcgatgtgg atgcctgtac agtcacgttt 13621 acgaacggga cgacacagtc tgctgacttg attgtcggag ctgatggtat taggtctacg 13681 attcgaagat tcgttctgga ggaggacgtg actgttccag ctagcggaat tgtcggcttt 13741 cgatggttgg tccaggccga tgctctcgac ccatatcccg aactggattg gattgtcaaa 13801 aaaccaccct tgggagccag gttgatttct actccacaga acccgcagag tggcgtagga 13861 ctggccgata gacgaacgat cattatttac gcatgcagag gtgggaccat ggttaatgta 13921 ttagccgtac acgacgacga aagagatcaa aacactgctg attggtccgt tcccgcatca 13981 aaggatgatt tattcagagt gtttcacgat taccatccaa gattccgacg actcttagag 14041 ttagcacagg acattaattt atggcagatg agggtggtcc cggtcttgaa aaaatgggtg 14101 aacaagaggg tatgcttgct gggtgatgcc gcccatgctt cactgcccac acttggacaa 14161 ggcttcggga tggggttgga ggatgcagtc gctttgggca cgttgttgcc taagggaacg 14221 accgcatcac agatcgagac aaggctggcc gtttacgaac agctgcgaaa ggatcgagct 14281 gagtttgtcg ccgcagagag ctatgaggag caatatgtcc cagagatgag agggctgtac 14341 cttaggtcca aagaattgag agacagagtg atgggttatg atattaaggt agagtcagag 14401 aaggttttgg aaacactgtt gcgatcatcc aactcagctt gagcttggac tcccatgttg 14461 gcaaaggcaa ccaaacaaac aatgaatgat ccgctcctgc atatggggcg gtttgagtat 14521 ttcaactgcc atttgggctg aattgtagac atgctcctgt cagaaattcc gtgatcttac 14581 tcaatattca gtaatctcgg ccaatatcct aaatgtgcgt ggctttatct gtctttgtat 14641 tgtttcatca attcatgtaa cgtttgcttt tcttatgaat tttcaaataa attatccgct 14701 gttagagctc ccggggcgcg ccctatggag gtcaacatgg tggagcacga cactctggtc 14761 tactccaaaa atgtcaaaga tacagtctca gaagatcaaa gggctattga gacttttcaa 14821 caaaggataa tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc 14881 gaaaggacag tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag 14941 gctatcattc aagatctctc tgccgacagt ggtcccaaag atggaccccc acccacgagg 15001 agcatcgtgg aaaaagaaga ggttccaacc acgtctacaa agcaagtgga ttgatgtgac 15061 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct 15121 atataaggaa gttcatttca tttggagagg acacgctact gtatttttac aacaattacc 15181 aacaacaaca aacaacaaac aacattacaa ttactattta caattacaat gaatagtagt 15241 aaaaaccctc catccactct gttagatgtt tttctggata ccgctaggaa ccttgataca 15301 gcatccagaa atgtcctgga gtgcggagaa cacaggtgga gttacagaga gttggacaca 15361 gtgtcatccg cattggccca acaccttcga tacactgtgg gtcttagccc cactgtagcc 15421 gtgattagcg agaatcaccc ctacatactt gcattaatgc tcgccgtatg gaaactcggc 15481 ggaacgttcg caccgataga tgttcattcc ccagccgaac ttgttgctgg aatgttgaat 15541 attgtaagcc cgtcatgtct tgtcattcct agctctgatg tgacaaatca gacattggca 15601 tgcgatctta atataccggt tgttgcattc cacccacatc aaagtacaat tcccgaactc 15661 aacaagaagt atctcacaga ttctcagatc agtcctgacc tgcccttccc cgaccctaat 15721 cgacctgctt tgtacttatt cacgtcatct gcaacgtcta ggtccaattt gaagtgcgtg 15781 ccacttacac acacattcat attgcgaaac tccttaagta aacgagcatg gtgcaaaaga 15841 atgaggccag agacagattt tgatggtatt agggtcctgg ggtgggcccc ctggagccat 15901 gtcctcgctc atatgcagga cattggccct cttacattat tgaatgccgg gtgctacgta 15961 tttgccacga ccccatctac ctacccgacg gagctgaagg atgaccgtga tgttattagt 16021 tgcgccgcaa atgccgtaat gtacaaaggt gttaaatcct tcgcatgcct cccattcgtg 16081 cttgggggac tcaaggcttt atgtgagtca gagcccagtg ttaaggcaca actgcaagtg 16141 gaggaacgag cccagctttt gaagtcactc caacacatga aaatcctgga atgcgggggg 16201 gcaatgctcg aagtgagtgt ggcaagctgg gctatagaga ataggattcc tatatctatt 16261 gggattggta tgacagagac aggcggcgct cttttcgctg ggccggtcca agcaattcaa 16321 accggcttta gcagtgaaga taaatttatc gaggacgcaa cctacttgct ggtcaaggac 16381 gattacgaga gccacgcaga ggaggacata aacgagggcg agttagtggt aaagtccaga 16441 atgttaccta ggggttattt gggctataac gacccctctt tctctgttga cgatgctggt 16501 tgggttactt tcaaaactgg agatcgatat tcagtcactc ctgacggcaa attctcctgg 16561 cttggtcgaa atactgattt cattcagatg acgagtggtg agactctcga tccccgtccg 16621 attgagtccc tcctttgtga gtccagcctt atatcacgag cctgtgtcat cggggataag 16681 ttcctgaacg gaccagccac agctgtatgc gctattatag aactcgaacc aacgactgtt 16741 gagaaaggcc aggctcacag cagggatatt gcccgaatct ttgcccccat caatcgagat 16801 cttcctcccc cgttacgtat agcatggagt cacgtacttg tacttcagcc aagcgagaaa 16861 ataccgatga cgaaaaaggg tacaatcttc cgtaagaaaa tcgaacaggt tttcggttcc 16921 gctttaggag gcagtagcgg cgacaactca caagcaacca ctgacgcttc agtagttagg 16981 agggatgagt tgagcaacac ggtcaaacat atcataagca gagttttggg agtctccgac 17041 gatgaactcc tttggacact gagctttgct gagcttggca tgacgtccgc tcttgctact 17101 agaatagcaa atgaattgaa cgaagttctg gtaggtgtga acctccccat caatgcatgc 17161 tacattcatg tggacctgcc ttctttgtca aatgccgtgt atgcaaagtt agcacacctt 17221 aaactcccgg ataggacgcc cgagccaagg aaggccccag ttgaaaatcc cggtggaaag 17281 gaaatcgtaa tagtaggtca ggcttttagg cttccgggga gtataaatga tgtcgcttct 17341 ttgagggacg cttttttggc tagacaagca tccagtatta ttactgaaat accgccagat 17401 agatgggacc acgcatcatt ttatccaaaa gacatacgat ttaataaagc agggctcgtc 17461 gacatcgcta actacgacca cagttttttc ggattgacag ccacggaggc actgtacctg 17521 tcaccaacga tgcgtttggc acttgaagtg tcctttgaag ccttggagaa tgctaatata 17581 cccgtgtccc agttaaaggg ctctcaaaca gctgtatatg tcgcaaccac cgacgacggg 17641 tttgagacac tgctgaatgc tgaggcagga tatgatgcat acacacgatt ctatggcact 17701 ggtagggccg cctctacggc cagtggacga attagttatt tactcgacgt acacggccct 17761 tcaattaccg tagacactgc ttgttcaggc ggtgctgttt gcatagacca ggccatagac 17821 tacctccaat cctctagcgc agctgatacc gccataatct gcgccagcaa cactcactgc 17881 tggccaggtt ccttcatgtt tctgagtgct caaggtatgg taagttccgg ggggcgatgt 17941 gcaactttta cgacggatgc cgacggctat gtaccctctg aaggtgctgt ggcatttatc 18001 ctcaaaaccc gagaagcagc tatgagagat aaagatacta tcttggcaac tatcaaagca 18061 acgcagatta gtcataatgg tcgttcccaa ggtttagtcg caccaaacgt caattcacaa 18121 gctgatttac acagaagctt attgcagaaa gctggtttga gtccagccga cattcacttc 18181 atcgaagctc acgggacagg aacgtcactc ggtgacctta gtgaaataca agcaattaat 18241 gatgcctaca cgtcatctca gcctcgaaca gccggaccct taatagtaag cgcaagtaaa 18301 accgtcattg gtcacacaga gcctgctgga ccgttggtag gcatgctctc agttctcaac 18361 tcctttaaag agggcgcagt tcccggctta gctcacctga cagcagataa cctcaatccg 18421 gctttagact gctcctccgt ccctttactt atcccctacc agcctgtcca cttggcagct 18481 cctaagccac acagggctgc tgtgatgagt tatggcttct ccggcactct tggaggtata 18541 gttcttgaag caccggatga ggaaaggctt gaggaagaac cacccaacga taagcctatg 18601 ttatttgttg ttagcgccaa aactcatact gcattgattg agtaccttgg acgatacctt 18661 gaatttctgc tccaggccaa tccccaagac ttctgcgata tctgttacac tagctgcgtt 18721 ggacgtgagc attacaggta ccgttttgca tgtgtggcaa acgatatgga agatttaatt 18781 ggccaacttc agaagaggct tgggtctaag gttccaccta aaccgtccta taaaagagga 18841 gccctcgctt ttgctttcag tggacaaggg acccagtttc gtggaatggc aacagaattg 18901 gctaaggcat atagtggttt tcgtaagata gtcagtgatc ttgctaaaag agcatcagaa 18961 ctgtccggcc atgcaatcga caggtttctg cttgcatatg acattggggc tgagaatgtt 19021 gcaccggata gtgaagctga tcaaatctgc atattcgttt accaatgtag tgttctcaga 19081 tggctccaaa caatggggat tcgtccgtca gccgtgatcg gtcacagcct cggtgagatt 19141 agcgcatccg tagctgctgg agcattgtcc ttggactctg cccttgacct cgtaataagt 19201 agagccaggt tactgaggtc atctacgaat gccccagctg gcatggcagc tatgtctgca 19261 tcccaggatg aagtagtcga actcatagga aagctcgatt tagataaagc taatagcctc 19321 agcgtgtccg taataaacgg cccccagaac accgttgttt caggatcttc tgcagcaata 19381 gaaagtatcg ttgctctcgc aaagggacga aaaatcaagg ccagcgcact caacattaac 19441 caggcattcc atagtcctta cgtcgattct gcagtacccg gcttacgagc ttggagtgag 19501 aaacatatat catcagctcg accattgcaa atccctttgt actctaccct cttgggcgct 19561 caagtttctg agggtcaaat gttaaatccc gatcactggg tagaccatgc taggaaaccc 19621 gttcagttcg cacaagcagc cactataatg aaggaatctt tcaccggagt aataatcgat 19681 attggacctc aagttgtagc atggtccctg ctcttaagca atggtcttac ttccgtgacc 19741 gcactcgccg caaagcgtgg ccgttcacag caagtggcct tcctctccgc cttagctgat 19801 ctttatcagg actacggcgt cgtaccagac tttgttggac tgtacgcaca acaggaggac 19861 gcttccagac tgaaaaagac cgatatctta acttacccct ttcagagggt tcgacgttac 19921 ccttccttta taccctctag aagggcaccc acccacgctc atgtccaaga cgaggaaact 19981 ctgtcatcag gatcatcaac tccaacctta gaaaatactg acctggacag tgggaaggaa 20041 tctctgatgg gaccaacaag agggcttttg cgagtcgatg atcttaggga ctcaatagtc 20101 agttcagtca aagatgtcct ggaattgaag agtaacgaag atctggacct cagcgagtcc 20161 cttaatgctc ttggcatgga ctctataatg ttcgctcaat tacgaaaaag gataggggag 20221 ggcctcggcc tgtccgttcc aatggttttt ctcagcgatg ccttcagtat tggagaaatg 20281 gtcagtaacc tcgtggaaca ggccgaagct tccgaggaca attgagcttc tctagctaga 20341 gtcgatcgac aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa 20401 ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat 20461 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta 20521 ctaaaatcca gatcgctgtt agagctctcc cggcgcgccc tatggagcag ttctttcaat 20581 gtaattccgc agtttagggc gtggccttgt atatcaaata attccaaaaa gtacgataaa 20641 ataggacata aacctaataa aaaatatctt gatacataat atacctctta ctcaatgcag 20701 ttaggggcgt ggtccttgta tattaattaa ttccaaaatt aagtacgaaa aaaattggac 20761 ataaacctaa taaaaaatat ctccatacat aatacctctt attcaacctt ctagtgctag 20821 attttcagtt taattggtaa caaatagtat ctacgttcac atggtttgac aaactcatca 20881 aagtaaacaa atcaaacgaa tgttttaact aagctgagaa taagatatca tgggtaaaca 20941 tagtacgaga ttacttccca tgtatgaaag gtcccgtaaa cgtggtgatg acgaaatggt 21001 tgagtaagcg tgcaatcaca gagacagtag atagtgtatg aaaatgggaa gaaatttaag 21061 atttaaaaaa aacacaaatc tttggatatg tggggcaaaa ggtttaaaga gcaagaagta 21121 tagtggaatg tttgaatttt attgacttag gtgtttgggt taacatcatt tctttctttc 21181 ccaccagtgt ttcaattgat ctgaaattta tgcgaaaaaa tttgcacttt ggatcgtaaa 21241 acacttccta tatatcaaat acgactccat atcaagagtt tatattttcc aacaacatac 21301 cgagtatagt cctacaagta ggatctggag agggtgatac gtacaaagac cttaccctca 21361 ccaaaagtgg agaggctgtt ttcgataaat tctcaactca atgaaagagt tcaaatttta 21421 gatacctcta attaattatg gaccaatatt tatctctcca cacaatcagc tatcattcac 21481 tgtcacactt cctttggtgg agtcgataac accagaaaag gaattgtagt tcatgagtag 21541 ctttcacaaa cccattggtg ggccatggaa tcttacattc tctctcacta tatatatagc 21601 catacacttc ccattaatca ataaatatat atgtttaaac aaaactttag agagaaagga 21661 gagaactaat tgcaagaaaa gttggtagat ttttttatat atttaggtgg gtagaagggc 21721 aataaaatta gcctacacaa tttctttttt gttggtggta gctgagagtc agtagtgaat 21781 gcgtataaat atctctctgt ctagcttatt cgagagatta agcaaactca gctctcgtag 21841 catagctatt acatgtggtg tcgtcctcgc ctctgctatt gcttttccca tcatacgacg 21901 agattatcag acgttcttag aggttggtcc tagctatgcc cctcaaaatt ttcgaggcta 21961 cattatcgtt tgcgtactca gcttgttccg acaagagcaa aaagggttgg caatctacga 22021 cagactccca gagaagagaa gatggctggc cgatctccca tttcgtgaag gtactagacc 22081 tagtataaca tcacacataa tacagagaca gcgaactcaa cttgtagacc aagagtttgc 22141 cacacgagaa ctgatagaca aagttattcc cagggtgcaa gcacgacata cagataaaac 22201 atttttgagc acgagcaagt ttgaatttca tgctaaagcc attttcctgt tgccaagtat 22261 tcctataaac gaccccttaa acatacctag tcatgatacg gttcgtagaa cgaaacgaga 22321 gattgcacac atgcatgact accacgactg tactcttcac ttagctcttg cagctcaaga 22381 tggaaaagaa gtcctgaaaa aaggctgggg ccaacgtcat ccgcttgccg ggccgggtgt 22441 acctgggccg ccgacagaat ggactttctt atacgcaccc cgtaatgagg aagaagctag 22501 ggtagtagaa atgattgtcg aggcaagtat cggatacatg actaatgatc cggcaggtaa 22561 aatcgtcgag aatgccaaat gagcttgtcc tgctttaatg agatatgcga gaagcctatg 22621 atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta 22681 gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg 22741 tatttttatg aataatattc tccgttcaat ttactgattg taccctacta cttatatgta 22801 caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca tctatgatag 22861 agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta aaaaaagcgg 22921 cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt caaaagtgcc 22981 ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac tgaagggaac 23041 tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt ggccgtccgc 23101 tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg gtaaccgact 23161 tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa tgaagtgcag 23221 gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac 23281 atgtcgaggc tcagcaggac cgctgttaga gctcagtg //
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇:IDP427
- 下一篇:pBS-ldhGFP