买家导航
pLVX-EGFP-C1载体质粒基本信息
pLVX-EGFP-C1质粒图谱载体图谱和pLVX-EGFP-C1载体序列质粒序列多克隆位点信息
pLVX-EGFP-C1质粒载体简介
pLVX-EGFP-C1质粒序列载体序列
LOCUS pLVX-EGFP-C1 8786 bp ds-DNA circular SYN 13-JAN-2022 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8786) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Jan 13, 2022 from SnapGene Viewer 5.1.4.1 to Vector NTI(R) format https://www.biofeng.com COMMENT VNTDATE|974332800| COMMENT VNTDBDATE|974332800| COMMENT VNTNAME|pLVX-EGFP-C1| FEATURES Location/Qualifiers LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" misc_feature 2028..2143 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" enhancer 2201..2504 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2505..2708 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" regulatory 2800..2809 /regulatory_class="other" /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2806..3522 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" misc_feature 3523..3588 /label=MCS /note="multiple cloning site" promoter 3621..4120 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" CDS 4141..4740 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" misc_feature 4754..5342 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(5225..5236) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site LTR 5549..6182 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(6311..6327) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 6335..6351 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6359..6389) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6404..6425 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(6713..7301) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7472..8332) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(8333..8437) /gene="bla" /label=AmpR promoter polyA_signal 8485..8619 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgacc gtcagatcgc ctggagacgc catccacgct gttttgacct 2761 ccatagaaga caccgactct actagaggat ctaccggtcg ccaccatggt gagcaagggc 2821 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 2881 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 2941 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 3001 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 3061 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 3121 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 3181 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 3241 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 3301 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 3361 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 3421 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 3481 accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga 3541 gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga 3601 taactgatca taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg 3661 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca 3721 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc 3781 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac 3841 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca 3901 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg 3961 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg 4021 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc 4081 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc 4141 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 4201 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 4261 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 4321 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 4381 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 4441 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 4501 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 4561 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 4621 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 4681 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 4741 ccgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 4801 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 4861 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 4921 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 4981 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 5041 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 5101 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 5161 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 5221 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 5281 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 5341 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat 5401 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt 5461 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 5521 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa 5581 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac 5641 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca 5701 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct 5761 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc 5821 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga 5881 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc 5941 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac 6001 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 6061 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 6121 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 6181 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 6241 agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc tagagcttgg 6301 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 6361 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 6421 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 6481 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 6541 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 6601 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 6661 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 6721 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 6781 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 6841 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 6901 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 6961 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 7021 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 7081 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 7141 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 7201 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 7261 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 7321 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 7381 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 7441 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 7501 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 7561 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 7621 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 7681 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 7741 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 7801 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 7861 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 7921 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 7981 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 8041 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 8101 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 8161 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 8221 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 8281 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 8341 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 8401 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 8461 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa 8521 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 8581 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac 8641 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt 8701 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt 8761 taaatatgta ctacaaactt agtagt //