pComb3XTT

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pComb3XTT 
出品公司: 爱迪基因
目录编号: 63891
存储实验室: Carlos Barbas

质粒骨架基本信息
质粒名称: pComb3X
质粒抗性: 氨苄霉素
空质粒大小: 3300 bp
完整质粒大小: 4700 bp
质粒类型: 细菌表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: TT Fab
物种来源: H. sapiens (human)
插入大小: 1400 bp
启动子 lacZ
标签/融合蛋白: 6xHis(C端)     HA tag(C端)       geneIII(C端)
克隆方法: 限制性内切酶
5' 克隆位点: SfiI(未破坏)
3' 克隆位点: SfiI(未破坏)
5' 测序引物 AAG ACA GCT ATC GCG ATT GCA G
3' 测序引物 GCC CCC TTA TTA GCG TTT GCC ATC

文献引用方法
材料和方法部分: pComb3XTT was a gift from Carlos Barbas (Addgene plasmid # 63891)
参考文献部分: Methods for the generation of chicken monoclonal antibody fragments by phage display. Andris-Widhopf J, Rader C, Steinberger P, Fuller R, Barbas CF 3rd. J Immunol Methods. 2000 Aug 28;242(1-2):159-81. S0022175900002210 [pii] PubMed 10986398

载体图谱质粒图谱和多克隆位点信息

pComb3XTT载体图谱

载体简介

载体序列

LOCUS       pComb3XTT	4766 bp 	DNA	SYN
DEFINITION  pComb3XTT
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="pComb3XTT"
                     /mol_type="other DNA"
     promoter        145..174
                     /label="lac_promoter"
     CDS             222..926
                     /label="ORF frame 3"
     CDS             957..1793
                     /label="ORF frame 3"
     misc_feature    1737..1754
                     /label="6xHIS"
     misc_feature    1761..1787
                     /label="HA"
     rep_origin      complement(2513..2819)
                     /label="f1_origin"
     promoter        2898..2926
                     /label="AmpR_promoter"
     gene            2968..3828
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             2968..3828
                     /label="ORF frame 1"
     rep_origin      3983..4602
                     /label="pBR322_origin"
ORIGIN
    1 AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG
   61 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA
  121 GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG
  181 AATTGTGAGC GGATAACAAT TGAATTCAGG AGGAATTTAA AATGAAAAAG ACAGCTATCG
  241 CGATTGCAGT GGCACTGGCT GGTTTCGCTA CCGTGGCCCA GGCGGCCGAG CTCACGCAGT
  301 CTCCAGGCAC CCTGTCTTTG TCTCCAGGGG AAAGAGCCAC CCTCTCCTGC AGGGCCAGTC
  361 ACAGTGTTAG CAGGGCCTAC TTAGCCTGGT ACCAGCAGAA ACCTGGCCAG GCTCCCAGGC
  421 TCCTCATCTA TGGTACATCC AGCAGGGCCA CTGGCATCCC AGACAGGTTC AGTGGCAGTG
  481 GGTCTGGGAC AGACTTCACT CTCACCATCA GCAGACTGGA GCCTGAAGAT TTTGCAGTGT
  541 ACTACTGTCA GCAGTATGGT GGCTCACCGT GGTTCGGCCA AGGGACCAAG GTGGAACTCA
  601 AACGAACTGT GGCTGCACCA TCTGTCTTCA TCTTCCCGCC ATCTGATGAG CAGTTGAAAT
  661 CTGGAACTGC CTCTGTTGTG TGCCTGCTGA ATAACTTCTA TCCCAGAGAG GCCAAAGTAC
  721 AGTGGAAGGT GGATAACGCC CTCCAATCGG GTAACTCCCA GGAGAGTGTC ACAGAGCAGG
  781 ACAGCAAGGA CAGCACCTAC AGCCTCAGCA GCACCCTGAC GCTGAGCAAA GCAGACTACG
  841 AGAAACACAA AGTCTACGCC TGCGAAGTCA CCCATCAGGG CCTGAGCTTG CCCGTCACAA
  901 AGAGCTTCAA CAGGGGAGAG TGTTAGTTCT AGATAATTAA TTAGGAGGAA TTTAAAATGA
  961 AATACCTATT GCCTACGGCA GCCGCTGGAT TGTTATTACT CGCTGCCCAA CCAGCCATGG
 1021 CCGAGGTGCA GCTGCTCGAG CAGTCTGGGG CTGAGGTGAA GAAGCCTGGG TCCTCGGTGA
 1081 AGGTCTCCTG CAGGGCTTCT GGAGGCACCT TCAACAATTA TGCCATCAGC TGGGTGCGAC
 1141 AGGCCCCTGG ACAAGGGCTT GAGTGGATGG GAGGGATCTT CCCTTTCCGT AATACAGCAA
 1201 AGTACGCACA ACACTTCCAG GGCAGAGTCA CCATTACCGC GGACGAATCC ACGGGCACAG
 1261 CCTACATGGA GCTGAGCAGC CTGAGATCTG AGGACACGGC CATATATTAT TGTGCGAGAG
 1321 GGGATACGAT TTTTGGAGTG ACCATGGGAT ACTACGCTAT GGACGTCTGG GGCCAAGGGA
 1381 CCACGGTCAC CGTCTCCGCA GCCTCCACCA AGGGCCCATC GGTCTTCCCC CTGGCACCCT
 1441 CCTCCAAGAG CACCTCTGGG GGCACAGCGG CCCTGGGCTG CCTGGTCAAG GACTACTTCC
 1501 CCGAACCGGT GACGGTGTCG TGGAACTCAG GCGCCCTGAC CAGCGGCGTG CACACCTTCC
 1561 CGGCTGTCCT ACAGTCCTCA GGACTCTACT CCCTCAGCAG CGTGGTGACC GTGCCCTCCA
 1621 GCAGCTTGGG CACCCAGACC TACATCTGCA ACGTGAATCA CAAGCCCAGC AACACCAAGG
 1681 TGGACAAGAA AGTTGAGCCC AAATCTTGTG ACAAAACTAG TGGCCAGGCC GGCCAGCACC
 1741 ATCACCATCA CCATGGCGCA TACCCGTACG ACGTTCCGGA CTACGCTTCT TAGGAGGGTG
 1801 GTGGCTCTGA GGGTGGCGGT TCTGAGGGTG GCGGCTCTGA GGGAGGCGGT TCCGGTGGTG
 1861 GCTCTGGTTC CGGTGATTTT GATTATGAAA AGATGGCAAA CGCTAATAAG GGGGCTATGA
 1921 CCGAAAATGC CGATGAAAAC GTGCTACAGT CTGACGCTAA AGGCAAACTT GATTCTGTCG
 1981 CTACTGATTA CGGTGCTGCT ATCGATGGTT TCATTGGTGA CGTTTCCGGC CTTGCTAATG
 2041 GTAATGGTGC TACTGGTGAT TTTGCTGGCT CTAATTCCCA AATGGCTCAA GTCGGTGACG
 2101 GTGATAATTC ACCTTTAATG AATAATTTCC GTCAATATTT ACCTTCCCTC CCTCAATCGG
 2161 TTGAATGTCG CCCTTTTGTC TTTGGCGCTG GTAAACCATA TGAATTTTCT ATTGATTGTG
 2221 ACAAAATAAA CTTATTCCGT GGTGTCTTTG CGTTTCTTTT ATATGTTGCC ACCTTTATGT
 2281 ATGTATTTTC TACGTTTGCT AACATACTGC GTAATAAGGA GTCTTAAGCT AGCTAATTAA
 2341 TTTAAGCGGC CGCAGATCTG CTCTCTGAGG AGGATCTGGG AAATTGTAAG CGTTAATATT
 2401 TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA
 2461 ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA
 2521 GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC
 2581 GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG
 2641 AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG
 2701 GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG
 2761 GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG
 2821 CCGCTACAGG GCGCGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT
 2881 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG
 2941 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT
 3001 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA
 3061 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC
 3121 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA
 3181 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC
 3241 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT
 3301 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT
 3361 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC
 3421 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA
 3481 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA
 3541 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG
 3601 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT
 3661 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT
 3721 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA
 3781 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA
 3841 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG
 3901 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC
 3961 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC
 4021 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT
 4081 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT
 4141 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT
 4201 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT
 4261 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG
 4321 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA
 4381 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG
 4441 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG
 4501 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC
 4561 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
 4621 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
 4681 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
 4741 AGCGAGTCAG TGAGCGAGGA AGCGGA
//

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