质粒名称: | pComb3XTT |
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出品公司: | 爱迪基因 |
目录编号: | 63891 |
存储实验室: | Carlos Barbas |
质粒骨架基本信息 |
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质粒名称: | pComb3X |
质粒抗性: | 氨苄霉素 |
空质粒大小: | 3300 bp |
完整质粒大小: | 4700 bp |
质粒类型: | 细菌表达 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | TT Fab |
物种来源: | H. sapiens (human) |
插入大小: | 1400 bp |
启动子 | lacZ |
标签/融合蛋白: | 6xHis(C端) HA tag(C端) geneIII(C端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | SfiI(未破坏) |
3' 克隆位点: | SfiI(未破坏) |
5' 测序引物 | AAG ACA GCT ATC GCG ATT GCA G |
3' 测序引物 | GCC CCC TTA TTA GCG TTT GCC ATC |
文献引用方法 |
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材料和方法部分: | pComb3XTT was a gift from Carlos Barbas (Addgene plasmid # 63891) |
参考文献部分: | Methods for the generation of chicken monoclonal antibody fragments by phage display. Andris-Widhopf J, Rader C, Steinberger P, Fuller R, Barbas CF 3rd. J Immunol Methods. 2000 Aug 28;242(1-2):159-81. S0022175900002210 [pii] PubMed 10986398 |
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载体简介
载体序列
LOCUS pComb3XTT 4766 bp DNA SYN DEFINITION pComb3XTT ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4766 /organism="pComb3XTT" /mol_type="other DNA" promoter 145..174 /label="lac_promoter" CDS 222..926 /label="ORF frame 3" CDS 957..1793 /label="ORF frame 3" misc_feature 1737..1754 /label="6xHIS" misc_feature 1761..1787 /label="HA" rep_origin complement(2513..2819) /label="f1_origin" promoter 2898..2926 /label="AmpR_promoter" gene 2968..3828 /label="Ampicillin" /gene="Ampicillin" CDS 2968..3828 /label="ORF frame 1" rep_origin 3983..4602 /label="pBR322_origin" ORIGIN 1 AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG 61 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA 121 GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG 181 AATTGTGAGC GGATAACAAT TGAATTCAGG AGGAATTTAA AATGAAAAAG ACAGCTATCG 241 CGATTGCAGT GGCACTGGCT GGTTTCGCTA CCGTGGCCCA GGCGGCCGAG CTCACGCAGT 301 CTCCAGGCAC CCTGTCTTTG TCTCCAGGGG AAAGAGCCAC CCTCTCCTGC AGGGCCAGTC 361 ACAGTGTTAG CAGGGCCTAC TTAGCCTGGT ACCAGCAGAA ACCTGGCCAG GCTCCCAGGC 421 TCCTCATCTA TGGTACATCC AGCAGGGCCA CTGGCATCCC AGACAGGTTC AGTGGCAGTG 481 GGTCTGGGAC AGACTTCACT CTCACCATCA GCAGACTGGA GCCTGAAGAT TTTGCAGTGT 541 ACTACTGTCA GCAGTATGGT GGCTCACCGT GGTTCGGCCA AGGGACCAAG GTGGAACTCA 601 AACGAACTGT GGCTGCACCA TCTGTCTTCA TCTTCCCGCC ATCTGATGAG CAGTTGAAAT 661 CTGGAACTGC CTCTGTTGTG TGCCTGCTGA ATAACTTCTA TCCCAGAGAG GCCAAAGTAC 721 AGTGGAAGGT GGATAACGCC CTCCAATCGG GTAACTCCCA GGAGAGTGTC ACAGAGCAGG 781 ACAGCAAGGA CAGCACCTAC AGCCTCAGCA GCACCCTGAC GCTGAGCAAA GCAGACTACG 841 AGAAACACAA AGTCTACGCC TGCGAAGTCA CCCATCAGGG CCTGAGCTTG CCCGTCACAA 901 AGAGCTTCAA CAGGGGAGAG TGTTAGTTCT AGATAATTAA TTAGGAGGAA TTTAAAATGA 961 AATACCTATT GCCTACGGCA GCCGCTGGAT TGTTATTACT CGCTGCCCAA CCAGCCATGG 1021 CCGAGGTGCA GCTGCTCGAG CAGTCTGGGG CTGAGGTGAA GAAGCCTGGG TCCTCGGTGA 1081 AGGTCTCCTG CAGGGCTTCT GGAGGCACCT TCAACAATTA TGCCATCAGC TGGGTGCGAC 1141 AGGCCCCTGG ACAAGGGCTT GAGTGGATGG GAGGGATCTT CCCTTTCCGT AATACAGCAA 1201 AGTACGCACA ACACTTCCAG GGCAGAGTCA CCATTACCGC GGACGAATCC ACGGGCACAG 1261 CCTACATGGA GCTGAGCAGC CTGAGATCTG AGGACACGGC CATATATTAT TGTGCGAGAG 1321 GGGATACGAT TTTTGGAGTG ACCATGGGAT ACTACGCTAT GGACGTCTGG GGCCAAGGGA 1381 CCACGGTCAC CGTCTCCGCA GCCTCCACCA AGGGCCCATC GGTCTTCCCC CTGGCACCCT 1441 CCTCCAAGAG CACCTCTGGG GGCACAGCGG CCCTGGGCTG CCTGGTCAAG GACTACTTCC 1501 CCGAACCGGT GACGGTGTCG TGGAACTCAG GCGCCCTGAC CAGCGGCGTG CACACCTTCC 1561 CGGCTGTCCT ACAGTCCTCA GGACTCTACT CCCTCAGCAG CGTGGTGACC GTGCCCTCCA 1621 GCAGCTTGGG CACCCAGACC TACATCTGCA ACGTGAATCA CAAGCCCAGC AACACCAAGG 1681 TGGACAAGAA AGTTGAGCCC AAATCTTGTG ACAAAACTAG TGGCCAGGCC GGCCAGCACC 1741 ATCACCATCA CCATGGCGCA TACCCGTACG ACGTTCCGGA CTACGCTTCT TAGGAGGGTG 1801 GTGGCTCTGA GGGTGGCGGT TCTGAGGGTG GCGGCTCTGA GGGAGGCGGT TCCGGTGGTG 1861 GCTCTGGTTC CGGTGATTTT GATTATGAAA AGATGGCAAA CGCTAATAAG GGGGCTATGA 1921 CCGAAAATGC CGATGAAAAC GTGCTACAGT CTGACGCTAA AGGCAAACTT GATTCTGTCG 1981 CTACTGATTA CGGTGCTGCT ATCGATGGTT TCATTGGTGA CGTTTCCGGC CTTGCTAATG 2041 GTAATGGTGC TACTGGTGAT TTTGCTGGCT CTAATTCCCA AATGGCTCAA GTCGGTGACG 2101 GTGATAATTC ACCTTTAATG AATAATTTCC GTCAATATTT ACCTTCCCTC CCTCAATCGG 2161 TTGAATGTCG CCCTTTTGTC TTTGGCGCTG GTAAACCATA TGAATTTTCT ATTGATTGTG 2221 ACAAAATAAA CTTATTCCGT GGTGTCTTTG CGTTTCTTTT ATATGTTGCC ACCTTTATGT 2281 ATGTATTTTC TACGTTTGCT AACATACTGC GTAATAAGGA GTCTTAAGCT AGCTAATTAA 2341 TTTAAGCGGC CGCAGATCTG CTCTCTGAGG AGGATCTGGG AAATTGTAAG CGTTAATATT 2401 TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA 2461 ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA 2521 GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC 2581 GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG 2641 AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG 2701 GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG 2761 GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG 2821 CCGCTACAGG GCGCGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 2881 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 2941 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 3001 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA 3061 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC 3121 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA 3181 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC 3241 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 3301 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT 3361 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC 3421 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 3481 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 3541 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 3601 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT 3661 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT 3721 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 3781 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA 3841 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG 3901 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 3961 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 4021 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 4081 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 4141 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 4201 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 4261 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 4321 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 4381 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 4441 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 4501 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 4561 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 4621 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 4681 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 4741 AGCGAGTCAG TGAGCGAGGA AGCGGA //
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