出品公司: | 爱迪基因 |
---|---|
载体名称: | pCMV-dR8.2-dvpr 其它名称 pCMVdR8.2 dvpr、 pHR' CMV 8.2 deltaR |
质粒类型: | 哺乳动物载体;第二代慢病毒包装;双质粒包装系统 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 13380 bp |
5' 测序引物及序列: | CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’ |
3' 测序引物及序列: | -- |
载体标签: | 无 |
载体抗性: | Ampicillin |
筛选标记: | 无 |
克隆菌株: | DH5α,HB101等 |
宿主细胞(系): | 包装细胞系如293T |
备注: |
第二代慢病毒包装载体pCMV-dR8.2-dvpr需要与信封载体 pCMV-VSVG 、pCMV-VSV-G 共同使用,使用方法见下文。用来包装第二代、第三代慢病毒表达载体。 |
产品目录号: | #8455 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
买家导航
pCMV-dR8.2-dvpr载体质粒基本信息
pCMV-dR8.2-dvpr质粒图谱载体图谱和pCMV-dR8.2-dvpr载体序列质粒序列多克隆位点信息
pCMV-dR8.2-dvpr质粒载体简介
双质粒系统慢病毒包装质粒pCMV-dR8.2-dvpr使用方法
Day 1
1. Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scaled up if desired)
Day 2
1. Set up (use polypropylene tubes for this; polystyrene tubes DO NOT work!):
1 µg retroviral DNA encoding gene X
1 µg packaging plasmid Mix
the packaging plasmid (pCMV-dR8.2-dvpr) at a 8:1 ratio with the envelope plasmid (pCVM-VSV-G)-a total of 1µg
DME without serum to 94μL total
6 μL Fugene
Mix and wait 15 to 30 minutes at room temperature
Add to 293T cells without touching the sides of the dish (DO NOT CHANGE MEDIA)
If you are using amphotropic virus then move immediately to BL2+ in a secondary container, which has an absorbent material.
(This does not mean a couple of hours; it means Immediately!). The rest of this protocol is the same for all viruses---the BL2+ safety practices are in place if you are using amphotropic viruses
Day 3
1. Change the media to whatever media you wish to use when infecting target cells. 293T cells are easily detached so remember not to put the media directly onto to cells, but rather “run” it down the side of the dish. Remember that you will get the highest titer virus when your cells are “happy.”
2. Plate out your target cells
Day 4
1. Remove the medium from the 293T cells and use a 0.45 u syringe filter to remove any 293T cells. DO NOT use the 0.2 u filter, as it is likely to shear the envelope from your virus
making it noninfectious
Note: After filtering, the filter should be removed and placed in the biohazard bag in the hood and the syringe rinsed with bleach and decontaminated for a minimum of 20 min. It is useful to place a plastic beaker with bleach in the hood in advance
2. Add 8 to 10 μg/ml of polybrene (Hexadimethrine bromide) or protamine sulfate to the target cells
3. Carry out infection for 1 to 4 hours. Remove virus and replace with fresh media
Note: If you wish to do a second infection the following day, it is important to put fresh media on the cells and not let the virus remain on the cells overnight. The media contains huge amounts of envelope, both associated and unassociated with viral particles, which will bind all the cell surface receptors required for virus adsorption, resulting in their down-regulation. Hence, if you don’t change the media after the initial infection, very few receptors will be available for the next round of infection. In addition, very few cells tolerate the presence of high levels of the VSV-G envelope for extended periods of time (i.e. a lot of your cells may die)
Day 5 +
1. Allow the cells to recover and begin to express the virus-encoded genes. The cells usually require 48 hours for this to occur
2. Add drug if you are scoring for the presence of a vector that carries a drug resistance marker. Prior to this step it is advisable that you titrate the drug to be used for selection in order to know precisely how much to add. In addition, it is necessary to bring an extra plate of uninfected cells (often referred to as “canaries”) which will function as a positive control in the kill assay. Add drug to both plates. When your canaries are dead, you can remove the drug.
pCMV-dR8.2-dvpr质粒序列载体序列
LOCUS pCMV-dR8.2 dvpr Sequencing Result 13380 bp DNA SYN DEFINITION pCMV-dR8.2 dvpr Sequencing Result ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..13380 /organism="pCMV-dR8.2 dvpr Sequencing Result" /mol_type="other DNA" promoter 27..603 /label="CMV_immearly_promoter" misc_feature 106..393 /label="CAG_enhancer" misc_feature 560..580 /label="CMV_fwd_primer" promoter 561..630 /label="CMV_promoter" promoter 573..692 /label="CMV2_promoter" misc_feature 604..623 /label="pCEP_fwd_primer" misc_feature 606..630 /label="LNCX_primer" gene 880..2382 /label="Gag_HIV (variant)" /gene="Gag_HIV (variant)" CDS 880..2382 /label="ORF frame 1" /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERF AVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALD KIEEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVE EKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPV HAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRM YSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTIL KALGPGATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKC FNCGKEGHIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQ SRPEPTAPPEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ*" CDS 2448..5186 /label="ORF frame 3" /translation="MNLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGP TPVNIIGRNLLTQIGCTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEIC TEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPH PAGLKQKKSVTVLDVGDAYFSVPLDKDFRKYTAFTIPSINNETPGIRYQYNVLPQGWK GSPAIFQCSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRW GFTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQI YAGIKVRQLCKLLRGTKALTEVVPLTEEAELELAENREILKEPVHGVYYDPSKDLIAE IQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHTNDVKQLTEAVQKIATESIVIWGKT PKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIIGAETFYVDG AANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQAIHLALQDSGLEVNIVTDSQYA LGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKLVSAGIRKVL FLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSP GIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTVHTD NGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNKELKKIIGQVRDQAEHLKT AVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRD PVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDED*" misc_feature 4875..4890 /label="cPPT" /translation="MNLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGP TPVNIIGRNLLTQIGCTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEIC TEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPH PAGLKQKKSVTVLDVGDAYFSVPLDKDFRKYTAFTIPSINNETPGIRYQYNVLPQGWK GSPAIFQCSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRW GFTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQI YAGIKVRQLCKLLRGTKALTEVVPLTEEAELELAENREILKEPVHGVYYDPSKDLIAE IQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHTNDVKQLTEAVQKIATESIVIWGKT PKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIIGAETFYVDG AANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQAIHLALQDSGLEVNIVTDSQYA LGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKLVSAGIRKVL FLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSP GIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTVHTD NGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNKELKKIIGQVRDQAEHLKT AVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRD PVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDED*" CDS 5131..5709 /label="ORF frame 1" /translation="MENRWQVMIVWQVDRMRINTWKRLVKHHMYISRKAKDWFYRHHY ESTNPKISSEVHIPLGDAKLVITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPDL ADQLIHLHYFDCFSESAIRNTILGRIVSPRCEYQAGHNKVGSLQYLALAALIKPKQIK PPLPSVRKLTEDRWNKPQKTKGHRGSHTMNGH*" CDS complement(6153..6656) /label="ORF frame 2" /translation="MPQTVSCNRCCCASIALSKLFCCCTIPDNNCLACTVSVIDAAPI VLPAAPKNPRNKAPIPTALFSLCTTLLFALVGATPNGSIFTTLYLYNSLLQLSLISPP PGLKISGRSTTDHQYPKEHGAPFPPPSPQVLILLLHSHCHCLLLFLLVYQLTCLFFFV LIFSYIL*" CDS 6361..7509 /label="ORF frame 1" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELK NSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL*" misc_feature 6483..6716 /label="RRE" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELK NSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL*" misc_feature 7239..7268 /label="HIV_Rev_NES" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELK NSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL*" CDS 7511..8131 /label="ORF frame 2" /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*" misc_feature 7783..7798 /label="cPPT" /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*" misc_feature 7783..7804 /label="U3PPT" /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*" misc_feature complement(8503..8520) /label="Sp6_primer" /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*" gene 8758..9021 /label="tet (300 - 563)" /gene="tet (300 - 563)" /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*" misc_feature complement(8810..8829) /label="pBRrevBam_primer" /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*" misc_feature 9162..9184 /label="pGEX_3_primer" /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*" promoter 9343..9371 /label="AmpR_promoter" /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*" gene 9413..10273 /label="Ampicillin" /gene="Ampicillin" /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*" CDS 9413..10273 /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 10428..11047 /label="pBR322_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature complement(11273..11293) /label="pBABE_3_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature complement(11279..11732) /label="SV40_enhancer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter 11291..11559 /label="SV40_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 11458..11535 /label="SV40_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 11520..11539 /label="SV40pro_F_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 12740..12755 /label="SV40_int" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 12761..12808 /label="SV40_3_splice" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" CDS 12885..13358 /label="ORF frame 3" /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*" misc_feature 12936..12956 /label="SV40 NLS" /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*" misc_feature 12942..12956 /label="NLS" /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*" ORIGIN 1 GGGCTGCAGG AATTCGAGCT CGCCCGACAT TGATTATTGA CTAGTTATTA ATAGTAATCA 61 ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA ACTTACGGTA 121 AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT 181 GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG 241 TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC 301 GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT 361 CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG 421 CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC 481 ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT 541 AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 601 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC GCCTGGAGAC GCCATCCACG CTGTTTTGAC 661 CTCCATAGAA GACACCGGGA CCGATCCAGC CTCCGCGGCC GGGAACGGTG CATTGGAACG 721 CGGATTCCCC GTGCCAAGAG TGACGTAAGT ACCGCCTATA GAGTCTATAG GCCCACCCCC 781 TTGGCTTCTT ATGCGACGGA TCGATCCCGT AATAAGCTTC GAGGTCCGCG GCCGCGTTGA 841 CGCGCACGGC AAGAGGCGAG GGGCGGCGAC TGGTGAGAGA TGGGTGCGAG AGCGTCAGTA 901 TTAAGCGGGG GAGAATTAGA TCGATGGGAA AAAATTCGGT TAAGGCCAGG GGGAAAGAAA 961 AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC TAGAACGATT CGCAGTTAAT 1021 CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC TGGGACAGCT ACAACCATCC 1081 CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA CAGTAGCAAC CCTCTATTGT 1141 GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT TAGACAAGAT AGAGGAAGAG 1201 CAAAACAAAA GTAAGAAAAA AGCACAGCAA GCAGCAGCTG ACACAGGACA CAGCAATCAG 1261 GTCAGCCAAA ATTACCCTAT AGTGCAGAAC ATCCAGGGGC AAATGGTACA TCAGGCCATA 1321 TCACCTAGAA CTTTAAATGC ATGGGTAAAA GTAGTAGAAG AGAAGGCTTT CAGCCCAGAA 1381 GTGATACCCA TGTTTTCAGC ATTATCAGAA GGAGCCACCC CACAAGATTT AAACACCATG 1441 CTAAACACAG TGGGGGGACA TCAAGCAGCC ATGCAAATGT TAAAAGAGAC CATCAATGAG 1501 GAAGCTGCAG AATGGGATAG AGTGCATCCA GTGCATGCAG GGCCTATTGC ACCAGGCCAG 1561 ATGAGAGAAC CAAGGGGAAG TGACATAGCA GGAACTACTA GTACCCTTCA GGAACAAATA 1621 GGATGGATGA CACATAATCC ACCTATCCCA GTAGGAGAAA TCTATAAAAG ATGGATAATC 1681 CTGGGATTAA ATAAAATAGT AAGAATGTAT AGCCCTACCA GCATTCTGGA CATAAGACAA 1741 GGACCAAAGG AACCCTTTAG AGACTATGTA GACCGATTCT ATAAAACTCT AAGAGCCGAG 1801 CAAGCTTCAC AAGAGGTAAA AAATTGGATG ACAGAAACCT TGTTGGTCCA AAATGCGAAC 1861 CCAGATTGTA AGACTATTTT AAAAGCATTG GGACCAGGAG CGACACTAGA AGAAATGATG 1921 ACAGCATGTC AGGGAGTGGG GGGACCCGGC CATAAAGCAA GAGTTTTGGC TGAAGCAATG 1981 AGCCAAGTAA CAAATCCAGC TACCATAATG ATACAGAAAG GCAATTTTAG GAACCAAAGA 2041 AAGACTGTTA AGTGTTTCAA TTGTGGCAAA GAAGGGCACA TAGCCAAAAA TTGCAGGGCC 2101 CCTAGGAAAA AGGGCTGTTG GAAATGTGGA AAGGAAGGAC ACCAAATGAA AGATTGTACT 2161 GAGAGACAGG CTAATTTTTT AGGGAAGATC TGGCCTTCCC ACAAGGGAAG GCCAGGGAAT 2221 TTTCTTCAGA GCAGACCAGA GCCAACAGCC CCACCAGAAG AGAGCTTCAG GTTTGGGGAA 2281 GAGACAACAA CTCCCTCTCA GAAGCAGGAG CCGATAGACA AGGAACTGTA TCCTTTAGCT 2341 TCCCTCAGAT CACTCTTTGG CAGCGACCCC TCGTCACAAT AAAGATAGGG GGGCAATTAA 2401 AGGAAGCTCT ATTAGATACA GGAGCAGATG ATACAGTATT AGAAGAAATG AATTTGCCAG 2461 GAAGATGGAA ACCAAAAATG ATAGGGGGAA TTGGAGGTTT TATCAAAGTA AGACAGTATG 2521 ATCAGATACT CATAGAAATC TGCGGACATA AAGCTATAGG TACAGTATTA GTAGGACCTA 2581 CACCTGTCAA CATAATTGGA AGAAATCTGT TGACTCAGAT TGGCTGCACT TTAAATTTTC 2641 CCATTAGTCC TATTGAGACT GTACCAGTAA AATTAAAGCC AGGAATGGAT GGCCCAAAAG 2701 TTAAACAATG GCCATTGACA GAAGAAAAAA TAAAAGCATT AGTAGAAATT TGTACAGAAA 2761 TGGAAAAGGA AGGAAAAATT TCAAAAATTG GGCCTGAAAA TCCATACAAT ACTCCAGTAT 2821 TTGCCATAAA GAAAAAAGAC AGTACTAAAT GGAGAAAATT AGTAGATTTC AGAGAACTTA 2881 ATAAGAGAAC TCAAGATTTC TGGGAAGTTC AATTAGGAAT ACCACATCCT GCAGGGTTAA 2941 AACAGAAAAA ATCAGTAACA GTACTGGATG TGGGCGATGC ATATTTTTCA GTTCCCTTAG 3001 ATAAAGACTT CAGGAAGTAT ACTGCATTTA CCATACCTAG TATAAACAAT GAGACACCAG 3061 GGATTAGATA TCAGTACAAT GTGCTTCCAC AGGGATGGAA AGGATCACCA GCAATATTCC 3121 AGTGTAGCAT GACAAAAATC TTAGAGCCTT TTAGAAAACA AAATCCAGAC ATAGTCATCT 3181 ATCAATACAT GGATGATTTG TATGTAGGAT CTGACTTAGA AATAGGGCAG CATAGAACAA 3241 AAATAGAGGA ACTGAGACAA CATCTGTTGA GGTGGGGATT TACCACACCA GACAAAAAAC 3301 ATCAGAAAGA ACCTCCATTC CTTTGGATGG GTTATGAACT CCATCCTGAT AAATGGACAG 3361 TACAGCCTAT AGTGCTGCCA GAAAAGGACA GCTGGACTGT CAATGACATA CAGAAATTAG 3421 TGGGAAAATT GAATTGGGCA AGTCAGATTT ATGCAGGGAT TAAAGTAAGG CAATTATGTA 3481 AACTTCTTAG GGGAACCAAA GCACTAACAG AAGTAGTACC ACTAACAGAA GAAGCAGAGC 3541 TAGAACTGGC AGAAAACAGG GAGATTCTAA AAGAACCGGT ACATGGAGTG TATTATGACC 3601 CATCAAAAGA CTTAATAGCA GAAATACAGA AGCAGGGGCA AGGCCAATGG ACATATCAAA 3661 TTTATCAAGA GCCATTTAAA AATCTGAAAA CAGGAAAGTA TGCAAGAATG AAGGGTGCCC 3721 ACACTAATGA TGTGAAACAA TTAACAGAGG CAGTACAAAA AATAGCCACA GAAAGCATAG 3781 TAATATGGGG AAAGACTCCT AAATTTAAAT TACCCATACA AAAGGAAACA TGGGAAGCAT 3841 GGTGGACAGA GTATTGGCAA GCCACCTGGA TTCCTGAGTG GGAGTTTGTC AATACCCCTC 3901 CCTTAGTGAA GTTATGGTAC CAGTTAGAGA AAGAACCCAT AATAGGAGCA GAAACTTTCT 3961 ATGTAGATGG GGCAGCCAAT AGGGAAACTA AATTAGGAAA AGCAGGATAT GTAACTGACA 4021 GAGGAAGACA AAAAGTTGTC CCCCTAACGG ACACAACAAA TCAGAAGACT GAGTTACAAG 4081 CAATTCATCT AGCTTTGCAG GATTCGGGAT TAGAAGTAAA CATAGTGACA GACTCACAAT 4141 ATGCATTGGG AATCATTCAA GCACAACCAG ATAAGAGTGA ATCAGAGTTA GTCAGTCAAA 4201 TAATAGAGCA GTTAATAAAA AAGGAAAAAG TCTACCTGGC ATGGGTACCA GCACACAAAG 4261 GAATTGGAGG AAATGAACAA GTAGATAAAT TGGTCAGTGC TGGAATCAGG AAAGTACTAT 4321 TTTTAGATGG AATAGATAAG GCCCAAGAAG AACATGAGAA ATATCACAGT AATTGGAGAG 4381 CAATGGCTAG TGATTTTAAC CTACCACCTG TAGTAGCAAA AGAAATAGTA GCCAGCTGTG 4441 ATAAATGTCA GCTAAAAGGG GAAGCCATGC ATGGACAAGT AGACTGTAGC CCAGGAATAT 4501 GGCAGCTAGA TTGTACACAT TTAGAAGGAA AAGTTATCTT GGTAGCAGTT CATGTAGCCA 4561 GTGGATATAT AGAAGCAGAA GTAATTCCAG CAGAGACAGG GCAAGAAACA GCATACTTCC 4621 TCTTAAAATT AGCAGGAAGA TGGCCAGTAA AAACAGTACA TACAGACAAT GGCAGCAATT 4681 TCACCAGTAC TACAGTTAAG GCCGCCTGTT GGTGGGCGGG GATCAAGCAG GAATTTGGCA 4741 TTCCCTACAA TCCCCAAAGT CAAGGAGTAA TAGAATCTAT GAATAAAGAA TTAAAGAAAA 4801 TTATAGGACA GGTAAGAGAT CAGGCTGAAC ATCTTAAGAC AGCAGTACAA ATGGCAGTAT 4861 TCATCCACAA TTTTAAAAGA AAAGGGGGGA TTGGGGGGTA CAGTGCAGGG GAAAGAATAG 4921 TAGACATAAT AGCAACAGAC ATACAAACTA AAGAATTACA AAAACAAATT ACAAAAATTC 4981 AAAATTTTCG GGTTTATTAC AGGGACAGCA GAGATCCAGT TTGGAAAGGA CCAGCAAAGC 5041 TCCTCTGGAA AGGTGAAGGG GCAGTAGTAA TACAAGATAA TAGTGACATA AAAGTAGTGC 5101 CAAGAAGAAA AGCAAAGATC ATCAGGGATT ATGGAAAACA GATGGCAGGT GATGATTGTG 5161 TGGCAAGTAG ACAGGATGAG GATTAACACA TGGAAAAGAT TAGTAAAACA CCATATGTAT 5221 ATTTCAAGGA AAGCTAAGGA CTGGTTTTAT AGACATCACT ATGAAAGTAC TAATCCAAAA 5281 ATAAGTTCAG AAGTACACAT CCCACTAGGG GATGCTAAAT TAGTAATAAC AACATATTGG 5341 GGTCTGCATA CAGGAGAAAG AGACTGGCAT TTGGGTCAGG GAGTCTCCAT AGAATGGAGG 5401 AAAAAGAGAT ATAGCACACA AGTAGACCCT GACCTAGCAG ACCAACTAAT TCATCTGCAC 5461 TATTTTGATT GTTTTTCAGA ATCTGCTATA AGAAATACCA TATTAGGACG TATAGTTAGT 5521 CCTAGGTGTG AATATCAAGC AGGACATAAC AAGGTAGGAT CTCTACAGTA CTTGGCACTA 5581 GCAGCATTAA TAAAACCAAA ACAGATAAAG CCACCTTTGC CTAGTGTTAG GAAACTGACA 5641 GAGGACAGAT GGAACAAGCC CCAGAAGACC AAGGGCCACA GAGGGAGCCA TACAATGAAT 5701 GGACACTAGA GCTTTAGTAG CCCGAACCCG GGGCGGACCG AAGTGGAAGC CATAATAAGA 5761 ATTCTGCAAC AACTGCTGTT TATCCATTTC AGAATTGGGT GTCGACATAG CAGAATAGGC 5821 GTTACTCGAC AGAGGAGAGC AAGAAATGGA GCCAGTAGAT CCTAGACTAG AGCCCTGGAA 5881 GCATCCAGGA AGTCAGCCTA AAACTGCTTG TACCAATTGC TATTGTAAAA AGTGTTGCTT 5941 TCATTGCCAA GTTTGTTTCA TGACAAAAGC CTTAGGCATC TCCTATGGCA GGAAGAAGCG 6001 GAGACAGCGA CGAAGAGCTC ATCAGAACAG TCAGACTCAT CAAGCTTCTC TATCAAAGCA 6061 GTAAGTAGTA CATGTAATGC AACCTATAAT AGTAGCAATA GTAGCATTAG TAGTAGCAAT 6121 AATAATAGCA ATAGTTGTGT GGTCCATAGT AATCATAGAA TATAGGAAAA TATTAAGACA 6181 AAGAAAAATA GACAGGTTAA TTGATAGACT AATAGAAAGA GCAGAAGACA GTGGCAATGA 6241 GAGTGAAGGA GAAGTATCAG CACTTGTGGA GATGGGGGTG GAAATGGGGC ACCATGCTCC 6301 TTGGGATATT GATGATCTGT AGTTGAGCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 6361 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 6421 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 6481 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 6541 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 6601 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 6661 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 6721 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 6781 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 6841 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 6901 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 6961 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 7021 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 7081 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 7141 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCCTTGGCA 7201 CTTATCTGGG ACGATCTGCG GAGCCTGTGC CTCTTCAGCT ACCACCGCTT GAGAGACTTA 7261 CTCTTGATTG TAACGAGGAT TGTGGAACTT CTGGGACGCA GGGGGTGGGA AGCCCTCAAA 7321 TATTGGTGGA ATCTCCTACA ATATTGGAGT CAGGAGCTAA AGAATAGTGC TGTTAGCTTG 7381 CTCAATGCCA CAGCCATAGC AGTAGCTGAG GGGACAGATA GGGTTATAGA AGTAGTACAA 7441 GGAGCTTGTA GAGCTATTCG CCACATACCT AGAAGAATAA GACAGGGCTT GGAAAGGATT 7501 TTGCTATAAG ATGGGTGGCA AGTGGTCAAA AAGTAGTGTG ATTGGATGGC CTACTGTAAG 7561 GGAAAGAATG AGACGAGCTG AGCCAGCAGC AGATGGGGTG GGAGCAGTAT CTCGAGACCT 7621 AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT ACCAATGCTG ATTGTGCCTG 7681 GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC ACACCTCAGG TACCTTTAAG 7741 ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT 7801 GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATATCCTT GATCTGTGGA TCTACCACAC 7861 ACAAGGCTAC TTCCCTGATT GGCAGAACTA CACACCAGGG CCAGGGATCA GATATCCACT 7921 GACCTTTGGA TGGTGCTACA AGCTAGTACC AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA 7981 TGAAGGAGAG AACACCCGCT TGTTACACCC TGTGAGCCTG CATGGGATGG ATGACCCGGA 8041 GAGAGAAGTA TTAGAGTGGA GGTTTGACAG CCGCCTAGCA TTTCATCACA TGGCCCGAGA 8101 GCTGCATCCG GAGTACTTCA AGAACTGCTG AGCGGCCGCC CCGGTGACCT TCAGACCTTG 8161 GCACTGGAGG TGGCCCGGCA GAAGCGCGGC ATCGTGGATC AGTGCTGCAC CAGCATCTGC 8221 TCTCTCTACC AACTGGAGAA CTACTGCAAC TAGGCCCACC ACTACCCTGT CCACCCCTCT 8281 GCAATGAATA AAACCTTTGA AAGAGCACTA CAAGTTGTGT GTACATGCGT GCATGTGCAT 8341 ATGTGGTGCG GGGGGAACAT GAGTGGGGCT GGCTGGAGTG GCGATGATAA GCTGTCAAAC 8401 ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA 8461 TGATAATAAT GGTTTCTTAG TCTAGAATTA ATTCCGTGTA TTCTATAGTG TCACCTAAAT 8521 CGTATGTGTA TGATACATAA GGTTATGTAT TAATTGTAGC CGCGTTCTAA CGACAATATG 8581 TACAAGCCTA ATTGTGTAGC ATCTGGCTTA CTGAAGCAGA CCCTATCATC TCTCTCGTAA 8641 ACTGCCGTCA GAGTCGGTTT GGTTGGACGA ACCTTCTGAG TTTCTGGTAA CGCCGTCCCG 8701 CACCCGGAAA TGGTCAGCGA ACCAATCAGC AGGGTCATCG CTAGCCAGAT CCTCTACGCC 8761 GGACGCATCG TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC 8821 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC 8881 GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA 8941 CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG 9001 CAGGAGTCGC ATAAGGGAGA GCGTCGAATG GTGCACTCTC AGTACAATCT GCTCTGATGC 9061 CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG 9121 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC Tccgggagct gcatgtgtca 9181 gaggttttca ccgtcatcac cgaaacgcgc gagACGAAAG GGCCTCGTGA TACGCCTATT 9241 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG 9301 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT 9361 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT 9421 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC 9481 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG 9541 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG 9601 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA 9661 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA 9721 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC 9781 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC 9841 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG 9901 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC 9961 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA 10021 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT 10081 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT 10141 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG 10201 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT 10261 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT 10321 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT 10381 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 10441 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 10501 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 10561 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 10621 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 10681 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 10741 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 10801 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 10861 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 10921 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 10981 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 11041 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC 11101 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC 11161 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC 11221 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GTGGAATGTG 11281 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 11341 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 11401 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 11461 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 11521 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 11581 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTTGGACACA AGACAGGCTT GCGAGATATG 11641 TTTGAGAATA CCACTTTATC CCGCGTCAGG GAGAGGCAGT GCGTAAAAAG ACGCGGACTC 11701 ATGTGAAATA CTGGTTTTTA GTGCGCCAGA TCTCTATAAT CTCGCGCAAC CTATTTTCCC 11761 CTCGAACACT TTTTAAGCCG TAGATAAACA GGCTGGGACA CTTCACATGA GCGAAAAATA 11821 CATCGTCACC TGGGACATGT TGCAGATCCA TGCACGTAAA CTCGCAAGCC GACTGATGCC 11881 TTCTGAACAA TGGAAAGGCA TTATTGCCGT AAGCCGTGGC GGTCTGTACC GGGTGCGTTA 11941 CTGGCGCGTG AACTGGGTAT TCGTCATGTC GATACCGTTT GTATTTCCAG CTACGATCAC 12001 GACAACCAGC GCGAGCTTAA AGTGCTGAAA CGCGCAGAAG GCGATGGCGA AGGCTTCATC 12061 GTTATTGATG ACCTGGTGGA TACCGGTGGT ACTGCGGTTG CGATTCGTGA AATGTATCCA 12121 AAAGCGCACT TTGTCACCAT CTTCGCAAAA CCGGCTGGTC GTCCGCTGGT TGATGACTAT 12181 GTTGTTGATA TCCCGCAAGA TACCTGGATT GAACAGCCGT GGGATATGGG CGTCGTATTC 12241 GTCCCGCCAA TCTCCGGTCG CTAATCTTTT CAACGCCTGG CACTGCCGGG CGTTGTTCTT 12301 TTTAACTTCA GGCGGGTTAC AATAGTTTCC AGTAAGTATT CTGGAGGCTG CATCCATGAC 12361 ACAGGCAAAC CTGAGCGAAA CCCTGTTCAA ACCCCGCTTT AAACATCCTG AAACCTCGAC 12421 GCTAGTCCGC CGCTTTAATC ACGGCGCACA ACCGCCTGTG CAGTCGGCCC TTGATGGTAA 12481 AACCATCCCT CACTGGTATC GCATGATTAA CCGTCTGATG TGGATCTGGC GCGGCATTGA 12541 CCCACGCGAA ATCCTCGACG TCCAGGCACG TATTGTGATG AGCGATGCCG AACGTACCGA 12601 CGATGATTTA TACGATACGG TGATTGGCTA CCGTGGCGGC AACTGGATTT ATGAGTGGGC 12661 CCCGGATCTT TGTGAAGGAA CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA 12721 CAGAGATTTA AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC 12781 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA TGGGAGCAGT 12841 GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA AGAAATGCCA TCTAGTGATG 12901 ATGAGGCTAC TGCTGACTCT CAACATTCTA CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG 12961 ACCCCAAGGA CTTTCCTTCA GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA 13021 GAACTCTTGC TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA 13081 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT AATCATAACA 13141 TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC TATTAATAAC TATGCTCAAA 13201 AATTGTGTAC CTTTAGCTTT TTAATTTGTA AAGGGGTTAA TAAGGAATAT TTGATGTATA 13261 GTGCCTTGAC TAGAGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA 13321 AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT //
pCMV-dR8.2-dvpr载体图谱质粒图谱pdf版和pCMV-dR8.2-dvpr载体序列质粒序列等相关资料下载
pCMV-dR8.2-dvpr质粒载体应用举例
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