pCMV-dR8.2-dvpr

价格:1250元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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pCMV-dR8.2-dvpr载体质粒基本信息

出品公司: 爱迪基因
载体名称: pCMV-dR8.2-dvpr   其它名称 pCMVdR8.2 dvpr、 pHR' CMV 8.2 deltaR
质粒类型: 哺乳动物载体;第二代慢病毒包装;双质粒包装系统
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 13380 bp
5' 测序引物及序列: CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
3' 测序引物及序列: --
载体标签:
载体抗性: Ampicillin
筛选标记:
克隆菌株: DH5α,HB101等
宿主细胞(系): 包装细胞系如293T
备注:
第二代慢病毒包装载体pCMV-dR8.2-dvpr需要与信封载体 pCMV-VSVGpCMV-VSV-G 共同使用,使用方法见下文。用来包装第二代、第三代慢病毒表达载体。
 
产品目录号: #8455
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pCMV-dR8.2-dvpr质粒图谱载体图谱和pCMV-dR8.2-dvpr载体序列质粒序列多克隆位点信息

pCMV-dR8.2-dvpr载体图谱



pCMV-dR8.2-dvpr质粒载体简介

双质粒系统慢病毒包装质粒pCMV-dR8.2-dvpr使用方法

Day 1

1.      Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scaled up if desired)


Day 2

1.      Set up (use polypropylene tubes for this; polystyrene tubes DO NOT work!):

           1 µg retroviral DNA encoding gene X
           1 µg packaging plasmid Mix
                the packaging plasmid (pCMV-dR8.2-dvpr) at a 8:1 ratio with the envelope plasmid (pCVM-VSV-G)-a total of 1µg
           DME without serum to 94μL total
           6 μL Fugene

       Mix and wait 15 to 30 minutes at room temperature

       Add to 293T cells without touching the sides of the dish (DO NOT CHANGE MEDIA)

       If you are using amphotropic virus then move immediately to BL2+ in a secondary container, which has an absorbent material. 

       (This does not mean a couple of hours; it means Immediately!).  The rest of this protocol is the same for all viruses---the BL2+ safety practices are in place if you are using amphotropic viruses 


Day 3

1.      Change the media to whatever media you wish to use when infecting target cells. 293T cells are easily detached so remember not to put the media directly onto to cells, but rather “run” it down the side of the dish. Remember that you will get the highest titer virus when your cells are “happy.”

2.      Plate out your target cells


Day 4

1.      Remove the medium from the 293T cells and use a 0.45 u syringe filter to remove any 293T cells. DO NOT use the 0.2 u filter, as it is likely to shear the envelope from your virus
making it noninfectious

        Note: After filtering, the filter should be removed and placed in the biohazard bag in the hood and the syringe rinsed with bleach and decontaminated for a minimum of 20 min. It is useful to place a plastic beaker with bleach in the hood in advance

2.      Add 8 to 10 μg/ml of polybrene (Hexadimethrine bromide) or protamine sulfate to the target cells

3.      Carry out infection for 1 to 4 hours. Remove virus and replace with fresh media

        Note: If you wish to do a second infection the following day, it is important to put fresh media on the cells and not let the virus remain on the cells overnight. The media contains huge amounts of envelope, both associated and unassociated with viral particles, which will bind all the cell surface receptors required for virus adsorption, resulting in their down-regulation. Hence, if you don’t change the media after the initial infection, very few receptors will be available for the next round of infection. In addition, very few cells tolerate the presence of high levels of the VSV-G envelope for extended periods of time (i.e. a lot of your cells may die)


Day 5 +

1.      Allow the cells to recover and begin to express the virus-encoded genes. The cells usually require 48 hours for this to occur

2.      Add drug if you are scoring for the presence of a vector that carries a drug resistance marker. Prior to this step it is advisable that you titrate the drug to be used for selection in order to know precisely how much to add. In addition, it is necessary to bring an extra plate of uninfected cells (often referred to as “canaries”) which will function as a positive control in the kill assay.  Add drug to both plates. When your canaries are dead, you can remove the drug.

pCMV-dR8.2-dvpr质粒序列载体序列

LOCUS       pCMV-dR8.2 dvpr Sequencing Result	13380 bp 	DNA	SYN
DEFINITION  pCMV-dR8.2 dvpr Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="pCMV-dR8.2 dvpr Sequencing Result"
                     /mol_type="other DNA"
     promoter        27..603
                     /label="CMV_immearly_promoter"
     misc_feature    106..393
                     /label="CAG_enhancer"
     misc_feature    560..580
                     /label="CMV_fwd_primer"
     promoter        561..630
                     /label="CMV_promoter"
     promoter        573..692
                     /label="CMV2_promoter"
     misc_feature    604..623
                     /label="pCEP_fwd_primer"
     misc_feature    606..630
                     /label="LNCX_primer"
     gene            880..2382
                     /label="Gag_HIV (variant)"
                     /gene="Gag_HIV (variant)"
     CDS             880..2382
                     /label="ORF frame 1"
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERF
                     AVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALD
                     KIEEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVE
                     EKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPV
                     HAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRM
                     YSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTIL
                     KALGPGATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKC
                     FNCGKEGHIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQ
                     SRPEPTAPPEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ*"
     CDS             2448..5186
                     /label="ORF frame 3"
                     /translation="MNLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGP
                     TPVNIIGRNLLTQIGCTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEIC
                     TEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPH
                     PAGLKQKKSVTVLDVGDAYFSVPLDKDFRKYTAFTIPSINNETPGIRYQYNVLPQGWK
                     GSPAIFQCSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRW
                     GFTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQI
                     YAGIKVRQLCKLLRGTKALTEVVPLTEEAELELAENREILKEPVHGVYYDPSKDLIAE
                     IQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHTNDVKQLTEAVQKIATESIVIWGKT
                     PKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIIGAETFYVDG
                     AANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQAIHLALQDSGLEVNIVTDSQYA
                     LGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKLVSAGIRKVL
                     FLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSP
                     GIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTVHTD
                     NGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNKELKKIIGQVRDQAEHLKT
                     AVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRD
                     PVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDED*"
     misc_feature    4875..4890
                     /label="cPPT"
                     /translation="MNLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGP
                     TPVNIIGRNLLTQIGCTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEIC
                     TEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPH
                     PAGLKQKKSVTVLDVGDAYFSVPLDKDFRKYTAFTIPSINNETPGIRYQYNVLPQGWK
                     GSPAIFQCSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRW
                     GFTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQI
                     YAGIKVRQLCKLLRGTKALTEVVPLTEEAELELAENREILKEPVHGVYYDPSKDLIAE
                     IQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHTNDVKQLTEAVQKIATESIVIWGKT
                     PKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIIGAETFYVDG
                     AANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQAIHLALQDSGLEVNIVTDSQYA
                     LGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKLVSAGIRKVL
                     FLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSP
                     GIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTVHTD
                     NGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNKELKKIIGQVRDQAEHLKT
                     AVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRD
                     PVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDED*"
     CDS             5131..5709
                     /label="ORF frame 1"
                     /translation="MENRWQVMIVWQVDRMRINTWKRLVKHHMYISRKAKDWFYRHHY
                     ESTNPKISSEVHIPLGDAKLVITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPDL
                     ADQLIHLHYFDCFSESAIRNTILGRIVSPRCEYQAGHNKVGSLQYLALAALIKPKQIK
                     PPLPSVRKLTEDRWNKPQKTKGHRGSHTMNGH*"
     CDS             complement(6153..6656)
                     /label="ORF frame 2"
                     /translation="MPQTVSCNRCCCASIALSKLFCCCTIPDNNCLACTVSVIDAAPI
                     VLPAAPKNPRNKAPIPTALFSLCTTLLFALVGATPNGSIFTTLYLYNSLLQLSLISPP
                     PGLKISGRSTTDHQYPKEHGAPFPPPSPQVLILLLHSHCHCLLLFLLVYQLTCLFFFV
                     LIFSYIL*"
     CDS             6361..7509
                     /label="ORF frame 1"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELK
                     NSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL*"
     misc_feature    6483..6716
                     /label="RRE"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELK
                     NSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL*"
     misc_feature    7239..7268
                     /label="HIV_Rev_NES"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELK
                     NSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL*"
     CDS             7511..8131
                     /label="ORF frame 2"
                     /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
                     SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
                     NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
     misc_feature    7783..7798
                     /label="cPPT"
                     /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
                     SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
                     NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
     misc_feature    7783..7804
                     /label="U3PPT"
                     /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
                     SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
                     NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
     misc_feature    complement(8503..8520)
                     /label="Sp6_primer"
                     /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
                     SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
                     NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
     gene            8758..9021
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
                     /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
                     SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
                     NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
     misc_feature    complement(8810..8829)
                     /label="pBRrevBam_primer"
                     /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
                     SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
                     NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
     misc_feature    9162..9184
                     /label="pGEX_3_primer"
                     /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
                     SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
                     NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
     promoter        9343..9371
                     /label="AmpR_promoter"
                     /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
                     SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
                     NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
     gene            9413..10273
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
                     SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
                     NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
     CDS             9413..10273
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      10428..11047
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(11273..11293)
                     /label="pBABE_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(11279..11732)
                     /label="SV40_enhancer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        11291..11559
                     /label="SV40_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      11458..11535
                     /label="SV40_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    11520..11539
                     /label="SV40pro_F_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    12740..12755
                     /label="SV40_int"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    12761..12808
                     /label="SV40_3_splice"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             12885..13358
                     /label="ORF frame 3"
                     /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS
                     NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN
                     YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*"
     misc_feature    12936..12956
                     /label="SV40 NLS"
                     /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS
                     NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN
                     YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*"
     misc_feature    12942..12956
                     /label="NLS"
                     /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS
                     NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN
                     YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*"
ORIGIN
    1 GGGCTGCAGG AATTCGAGCT CGCCCGACAT TGATTATTGA CTAGTTATTA ATAGTAATCA
   61 ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA ACTTACGGTA
  121 AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT
  181 GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG
  241 TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC
  301 GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT
  361 CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG
  421 CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC
  481 ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT
  541 AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
  601 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC GCCTGGAGAC GCCATCCACG CTGTTTTGAC
  661 CTCCATAGAA GACACCGGGA CCGATCCAGC CTCCGCGGCC GGGAACGGTG CATTGGAACG
  721 CGGATTCCCC GTGCCAAGAG TGACGTAAGT ACCGCCTATA GAGTCTATAG GCCCACCCCC
  781 TTGGCTTCTT ATGCGACGGA TCGATCCCGT AATAAGCTTC GAGGTCCGCG GCCGCGTTGA
  841 CGCGCACGGC AAGAGGCGAG GGGCGGCGAC TGGTGAGAGA TGGGTGCGAG AGCGTCAGTA
  901 TTAAGCGGGG GAGAATTAGA TCGATGGGAA AAAATTCGGT TAAGGCCAGG GGGAAAGAAA
  961 AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC TAGAACGATT CGCAGTTAAT
 1021 CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC TGGGACAGCT ACAACCATCC
 1081 CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA CAGTAGCAAC CCTCTATTGT
 1141 GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT TAGACAAGAT AGAGGAAGAG
 1201 CAAAACAAAA GTAAGAAAAA AGCACAGCAA GCAGCAGCTG ACACAGGACA CAGCAATCAG
 1261 GTCAGCCAAA ATTACCCTAT AGTGCAGAAC ATCCAGGGGC AAATGGTACA TCAGGCCATA
 1321 TCACCTAGAA CTTTAAATGC ATGGGTAAAA GTAGTAGAAG AGAAGGCTTT CAGCCCAGAA
 1381 GTGATACCCA TGTTTTCAGC ATTATCAGAA GGAGCCACCC CACAAGATTT AAACACCATG
 1441 CTAAACACAG TGGGGGGACA TCAAGCAGCC ATGCAAATGT TAAAAGAGAC CATCAATGAG
 1501 GAAGCTGCAG AATGGGATAG AGTGCATCCA GTGCATGCAG GGCCTATTGC ACCAGGCCAG
 1561 ATGAGAGAAC CAAGGGGAAG TGACATAGCA GGAACTACTA GTACCCTTCA GGAACAAATA
 1621 GGATGGATGA CACATAATCC ACCTATCCCA GTAGGAGAAA TCTATAAAAG ATGGATAATC
 1681 CTGGGATTAA ATAAAATAGT AAGAATGTAT AGCCCTACCA GCATTCTGGA CATAAGACAA
 1741 GGACCAAAGG AACCCTTTAG AGACTATGTA GACCGATTCT ATAAAACTCT AAGAGCCGAG
 1801 CAAGCTTCAC AAGAGGTAAA AAATTGGATG ACAGAAACCT TGTTGGTCCA AAATGCGAAC
 1861 CCAGATTGTA AGACTATTTT AAAAGCATTG GGACCAGGAG CGACACTAGA AGAAATGATG
 1921 ACAGCATGTC AGGGAGTGGG GGGACCCGGC CATAAAGCAA GAGTTTTGGC TGAAGCAATG
 1981 AGCCAAGTAA CAAATCCAGC TACCATAATG ATACAGAAAG GCAATTTTAG GAACCAAAGA
 2041 AAGACTGTTA AGTGTTTCAA TTGTGGCAAA GAAGGGCACA TAGCCAAAAA TTGCAGGGCC
 2101 CCTAGGAAAA AGGGCTGTTG GAAATGTGGA AAGGAAGGAC ACCAAATGAA AGATTGTACT
 2161 GAGAGACAGG CTAATTTTTT AGGGAAGATC TGGCCTTCCC ACAAGGGAAG GCCAGGGAAT
 2221 TTTCTTCAGA GCAGACCAGA GCCAACAGCC CCACCAGAAG AGAGCTTCAG GTTTGGGGAA
 2281 GAGACAACAA CTCCCTCTCA GAAGCAGGAG CCGATAGACA AGGAACTGTA TCCTTTAGCT
 2341 TCCCTCAGAT CACTCTTTGG CAGCGACCCC TCGTCACAAT AAAGATAGGG GGGCAATTAA
 2401 AGGAAGCTCT ATTAGATACA GGAGCAGATG ATACAGTATT AGAAGAAATG AATTTGCCAG
 2461 GAAGATGGAA ACCAAAAATG ATAGGGGGAA TTGGAGGTTT TATCAAAGTA AGACAGTATG
 2521 ATCAGATACT CATAGAAATC TGCGGACATA AAGCTATAGG TACAGTATTA GTAGGACCTA
 2581 CACCTGTCAA CATAATTGGA AGAAATCTGT TGACTCAGAT TGGCTGCACT TTAAATTTTC
 2641 CCATTAGTCC TATTGAGACT GTACCAGTAA AATTAAAGCC AGGAATGGAT GGCCCAAAAG
 2701 TTAAACAATG GCCATTGACA GAAGAAAAAA TAAAAGCATT AGTAGAAATT TGTACAGAAA
 2761 TGGAAAAGGA AGGAAAAATT TCAAAAATTG GGCCTGAAAA TCCATACAAT ACTCCAGTAT
 2821 TTGCCATAAA GAAAAAAGAC AGTACTAAAT GGAGAAAATT AGTAGATTTC AGAGAACTTA
 2881 ATAAGAGAAC TCAAGATTTC TGGGAAGTTC AATTAGGAAT ACCACATCCT GCAGGGTTAA
 2941 AACAGAAAAA ATCAGTAACA GTACTGGATG TGGGCGATGC ATATTTTTCA GTTCCCTTAG
 3001 ATAAAGACTT CAGGAAGTAT ACTGCATTTA CCATACCTAG TATAAACAAT GAGACACCAG
 3061 GGATTAGATA TCAGTACAAT GTGCTTCCAC AGGGATGGAA AGGATCACCA GCAATATTCC
 3121 AGTGTAGCAT GACAAAAATC TTAGAGCCTT TTAGAAAACA AAATCCAGAC ATAGTCATCT
 3181 ATCAATACAT GGATGATTTG TATGTAGGAT CTGACTTAGA AATAGGGCAG CATAGAACAA
 3241 AAATAGAGGA ACTGAGACAA CATCTGTTGA GGTGGGGATT TACCACACCA GACAAAAAAC
 3301 ATCAGAAAGA ACCTCCATTC CTTTGGATGG GTTATGAACT CCATCCTGAT AAATGGACAG
 3361 TACAGCCTAT AGTGCTGCCA GAAAAGGACA GCTGGACTGT CAATGACATA CAGAAATTAG
 3421 TGGGAAAATT GAATTGGGCA AGTCAGATTT ATGCAGGGAT TAAAGTAAGG CAATTATGTA
 3481 AACTTCTTAG GGGAACCAAA GCACTAACAG AAGTAGTACC ACTAACAGAA GAAGCAGAGC
 3541 TAGAACTGGC AGAAAACAGG GAGATTCTAA AAGAACCGGT ACATGGAGTG TATTATGACC
 3601 CATCAAAAGA CTTAATAGCA GAAATACAGA AGCAGGGGCA AGGCCAATGG ACATATCAAA
 3661 TTTATCAAGA GCCATTTAAA AATCTGAAAA CAGGAAAGTA TGCAAGAATG AAGGGTGCCC
 3721 ACACTAATGA TGTGAAACAA TTAACAGAGG CAGTACAAAA AATAGCCACA GAAAGCATAG
 3781 TAATATGGGG AAAGACTCCT AAATTTAAAT TACCCATACA AAAGGAAACA TGGGAAGCAT
 3841 GGTGGACAGA GTATTGGCAA GCCACCTGGA TTCCTGAGTG GGAGTTTGTC AATACCCCTC
 3901 CCTTAGTGAA GTTATGGTAC CAGTTAGAGA AAGAACCCAT AATAGGAGCA GAAACTTTCT
 3961 ATGTAGATGG GGCAGCCAAT AGGGAAACTA AATTAGGAAA AGCAGGATAT GTAACTGACA
 4021 GAGGAAGACA AAAAGTTGTC CCCCTAACGG ACACAACAAA TCAGAAGACT GAGTTACAAG
 4081 CAATTCATCT AGCTTTGCAG GATTCGGGAT TAGAAGTAAA CATAGTGACA GACTCACAAT
 4141 ATGCATTGGG AATCATTCAA GCACAACCAG ATAAGAGTGA ATCAGAGTTA GTCAGTCAAA
 4201 TAATAGAGCA GTTAATAAAA AAGGAAAAAG TCTACCTGGC ATGGGTACCA GCACACAAAG
 4261 GAATTGGAGG AAATGAACAA GTAGATAAAT TGGTCAGTGC TGGAATCAGG AAAGTACTAT
 4321 TTTTAGATGG AATAGATAAG GCCCAAGAAG AACATGAGAA ATATCACAGT AATTGGAGAG
 4381 CAATGGCTAG TGATTTTAAC CTACCACCTG TAGTAGCAAA AGAAATAGTA GCCAGCTGTG
 4441 ATAAATGTCA GCTAAAAGGG GAAGCCATGC ATGGACAAGT AGACTGTAGC CCAGGAATAT
 4501 GGCAGCTAGA TTGTACACAT TTAGAAGGAA AAGTTATCTT GGTAGCAGTT CATGTAGCCA
 4561 GTGGATATAT AGAAGCAGAA GTAATTCCAG CAGAGACAGG GCAAGAAACA GCATACTTCC
 4621 TCTTAAAATT AGCAGGAAGA TGGCCAGTAA AAACAGTACA TACAGACAAT GGCAGCAATT
 4681 TCACCAGTAC TACAGTTAAG GCCGCCTGTT GGTGGGCGGG GATCAAGCAG GAATTTGGCA
 4741 TTCCCTACAA TCCCCAAAGT CAAGGAGTAA TAGAATCTAT GAATAAAGAA TTAAAGAAAA
 4801 TTATAGGACA GGTAAGAGAT CAGGCTGAAC ATCTTAAGAC AGCAGTACAA ATGGCAGTAT
 4861 TCATCCACAA TTTTAAAAGA AAAGGGGGGA TTGGGGGGTA CAGTGCAGGG GAAAGAATAG
 4921 TAGACATAAT AGCAACAGAC ATACAAACTA AAGAATTACA AAAACAAATT ACAAAAATTC
 4981 AAAATTTTCG GGTTTATTAC AGGGACAGCA GAGATCCAGT TTGGAAAGGA CCAGCAAAGC
 5041 TCCTCTGGAA AGGTGAAGGG GCAGTAGTAA TACAAGATAA TAGTGACATA AAAGTAGTGC
 5101 CAAGAAGAAA AGCAAAGATC ATCAGGGATT ATGGAAAACA GATGGCAGGT GATGATTGTG
 5161 TGGCAAGTAG ACAGGATGAG GATTAACACA TGGAAAAGAT TAGTAAAACA CCATATGTAT
 5221 ATTTCAAGGA AAGCTAAGGA CTGGTTTTAT AGACATCACT ATGAAAGTAC TAATCCAAAA
 5281 ATAAGTTCAG AAGTACACAT CCCACTAGGG GATGCTAAAT TAGTAATAAC AACATATTGG
 5341 GGTCTGCATA CAGGAGAAAG AGACTGGCAT TTGGGTCAGG GAGTCTCCAT AGAATGGAGG
 5401 AAAAAGAGAT ATAGCACACA AGTAGACCCT GACCTAGCAG ACCAACTAAT TCATCTGCAC
 5461 TATTTTGATT GTTTTTCAGA ATCTGCTATA AGAAATACCA TATTAGGACG TATAGTTAGT
 5521 CCTAGGTGTG AATATCAAGC AGGACATAAC AAGGTAGGAT CTCTACAGTA CTTGGCACTA
 5581 GCAGCATTAA TAAAACCAAA ACAGATAAAG CCACCTTTGC CTAGTGTTAG GAAACTGACA
 5641 GAGGACAGAT GGAACAAGCC CCAGAAGACC AAGGGCCACA GAGGGAGCCA TACAATGAAT
 5701 GGACACTAGA GCTTTAGTAG CCCGAACCCG GGGCGGACCG AAGTGGAAGC CATAATAAGA
 5761 ATTCTGCAAC AACTGCTGTT TATCCATTTC AGAATTGGGT GTCGACATAG CAGAATAGGC
 5821 GTTACTCGAC AGAGGAGAGC AAGAAATGGA GCCAGTAGAT CCTAGACTAG AGCCCTGGAA
 5881 GCATCCAGGA AGTCAGCCTA AAACTGCTTG TACCAATTGC TATTGTAAAA AGTGTTGCTT
 5941 TCATTGCCAA GTTTGTTTCA TGACAAAAGC CTTAGGCATC TCCTATGGCA GGAAGAAGCG
 6001 GAGACAGCGA CGAAGAGCTC ATCAGAACAG TCAGACTCAT CAAGCTTCTC TATCAAAGCA
 6061 GTAAGTAGTA CATGTAATGC AACCTATAAT AGTAGCAATA GTAGCATTAG TAGTAGCAAT
 6121 AATAATAGCA ATAGTTGTGT GGTCCATAGT AATCATAGAA TATAGGAAAA TATTAAGACA
 6181 AAGAAAAATA GACAGGTTAA TTGATAGACT AATAGAAAGA GCAGAAGACA GTGGCAATGA
 6241 GAGTGAAGGA GAAGTATCAG CACTTGTGGA GATGGGGGTG GAAATGGGGC ACCATGCTCC
 6301 TTGGGATATT GATGATCTGT AGTTGAGCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT
 6361 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA
 6421 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA
 6481 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA
 6541 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT
 6601 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG
 6661 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG
 6721 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG
 6781 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA
 6841 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA
 6901 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC
 6961 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT
 7021 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA
 7081 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA
 7141 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCCTTGGCA
 7201 CTTATCTGGG ACGATCTGCG GAGCCTGTGC CTCTTCAGCT ACCACCGCTT GAGAGACTTA
 7261 CTCTTGATTG TAACGAGGAT TGTGGAACTT CTGGGACGCA GGGGGTGGGA AGCCCTCAAA
 7321 TATTGGTGGA ATCTCCTACA ATATTGGAGT CAGGAGCTAA AGAATAGTGC TGTTAGCTTG
 7381 CTCAATGCCA CAGCCATAGC AGTAGCTGAG GGGACAGATA GGGTTATAGA AGTAGTACAA
 7441 GGAGCTTGTA GAGCTATTCG CCACATACCT AGAAGAATAA GACAGGGCTT GGAAAGGATT
 7501 TTGCTATAAG ATGGGTGGCA AGTGGTCAAA AAGTAGTGTG ATTGGATGGC CTACTGTAAG
 7561 GGAAAGAATG AGACGAGCTG AGCCAGCAGC AGATGGGGTG GGAGCAGTAT CTCGAGACCT
 7621 AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT ACCAATGCTG ATTGTGCCTG
 7681 GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC ACACCTCAGG TACCTTTAAG
 7741 ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT
 7801 GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATATCCTT GATCTGTGGA TCTACCACAC
 7861 ACAAGGCTAC TTCCCTGATT GGCAGAACTA CACACCAGGG CCAGGGATCA GATATCCACT
 7921 GACCTTTGGA TGGTGCTACA AGCTAGTACC AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA
 7981 TGAAGGAGAG AACACCCGCT TGTTACACCC TGTGAGCCTG CATGGGATGG ATGACCCGGA
 8041 GAGAGAAGTA TTAGAGTGGA GGTTTGACAG CCGCCTAGCA TTTCATCACA TGGCCCGAGA
 8101 GCTGCATCCG GAGTACTTCA AGAACTGCTG AGCGGCCGCC CCGGTGACCT TCAGACCTTG
 8161 GCACTGGAGG TGGCCCGGCA GAAGCGCGGC ATCGTGGATC AGTGCTGCAC CAGCATCTGC
 8221 TCTCTCTACC AACTGGAGAA CTACTGCAAC TAGGCCCACC ACTACCCTGT CCACCCCTCT
 8281 GCAATGAATA AAACCTTTGA AAGAGCACTA CAAGTTGTGT GTACATGCGT GCATGTGCAT
 8341 ATGTGGTGCG GGGGGAACAT GAGTGGGGCT GGCTGGAGTG GCGATGATAA GCTGTCAAAC
 8401 ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA
 8461 TGATAATAAT GGTTTCTTAG TCTAGAATTA ATTCCGTGTA TTCTATAGTG TCACCTAAAT
 8521 CGTATGTGTA TGATACATAA GGTTATGTAT TAATTGTAGC CGCGTTCTAA CGACAATATG
 8581 TACAAGCCTA ATTGTGTAGC ATCTGGCTTA CTGAAGCAGA CCCTATCATC TCTCTCGTAA
 8641 ACTGCCGTCA GAGTCGGTTT GGTTGGACGA ACCTTCTGAG TTTCTGGTAA CGCCGTCCCG
 8701 CACCCGGAAA TGGTCAGCGA ACCAATCAGC AGGGTCATCG CTAGCCAGAT CCTCTACGCC
 8761 GGACGCATCG TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC
 8821 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC
 8881 GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA
 8941 CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG
 9001 CAGGAGTCGC ATAAGGGAGA GCGTCGAATG GTGCACTCTC AGTACAATCT GCTCTGATGC
 9061 CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG
 9121 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC Tccgggagct gcatgtgtca
 9181 gaggttttca ccgtcatcac cgaaacgcgc gagACGAAAG GGCCTCGTGA TACGCCTATT
 9241 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG
 9301 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
 9361 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
 9421 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
 9481 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
 9541 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
 9601 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
 9661 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
 9721 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
 9781 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
 9841 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
 9901 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
 9961 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
10021 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
10081 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT
10141 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
10201 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
10261 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
10321 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
10381 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
10441 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
10501 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
10561 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
10621 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
10681 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
10741 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
10801 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
10861 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
10921 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
10981 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
11041 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC
11101 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC
11161 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC
11221 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GTGGAATGTG
11281 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG
11341 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT
11401 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC
11461 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT
11521 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC
11581 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTTGGACACA AGACAGGCTT GCGAGATATG
11641 TTTGAGAATA CCACTTTATC CCGCGTCAGG GAGAGGCAGT GCGTAAAAAG ACGCGGACTC
11701 ATGTGAAATA CTGGTTTTTA GTGCGCCAGA TCTCTATAAT CTCGCGCAAC CTATTTTCCC
11761 CTCGAACACT TTTTAAGCCG TAGATAAACA GGCTGGGACA CTTCACATGA GCGAAAAATA
11821 CATCGTCACC TGGGACATGT TGCAGATCCA TGCACGTAAA CTCGCAAGCC GACTGATGCC
11881 TTCTGAACAA TGGAAAGGCA TTATTGCCGT AAGCCGTGGC GGTCTGTACC GGGTGCGTTA
11941 CTGGCGCGTG AACTGGGTAT TCGTCATGTC GATACCGTTT GTATTTCCAG CTACGATCAC
12001 GACAACCAGC GCGAGCTTAA AGTGCTGAAA CGCGCAGAAG GCGATGGCGA AGGCTTCATC
12061 GTTATTGATG ACCTGGTGGA TACCGGTGGT ACTGCGGTTG CGATTCGTGA AATGTATCCA
12121 AAAGCGCACT TTGTCACCAT CTTCGCAAAA CCGGCTGGTC GTCCGCTGGT TGATGACTAT
12181 GTTGTTGATA TCCCGCAAGA TACCTGGATT GAACAGCCGT GGGATATGGG CGTCGTATTC
12241 GTCCCGCCAA TCTCCGGTCG CTAATCTTTT CAACGCCTGG CACTGCCGGG CGTTGTTCTT
12301 TTTAACTTCA GGCGGGTTAC AATAGTTTCC AGTAAGTATT CTGGAGGCTG CATCCATGAC
12361 ACAGGCAAAC CTGAGCGAAA CCCTGTTCAA ACCCCGCTTT AAACATCCTG AAACCTCGAC
12421 GCTAGTCCGC CGCTTTAATC ACGGCGCACA ACCGCCTGTG CAGTCGGCCC TTGATGGTAA
12481 AACCATCCCT CACTGGTATC GCATGATTAA CCGTCTGATG TGGATCTGGC GCGGCATTGA
12541 CCCACGCGAA ATCCTCGACG TCCAGGCACG TATTGTGATG AGCGATGCCG AACGTACCGA
12601 CGATGATTTA TACGATACGG TGATTGGCTA CCGTGGCGGC AACTGGATTT ATGAGTGGGC
12661 CCCGGATCTT TGTGAAGGAA CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA
12721 CAGAGATTTA AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
12781 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA TGGGAGCAGT
12841 GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA AGAAATGCCA TCTAGTGATG
12901 ATGAGGCTAC TGCTGACTCT CAACATTCTA CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG
12961 ACCCCAAGGA CTTTCCTTCA GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA
13021 GAACTCTTGC TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
13081 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT AATCATAACA
13141 TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC TATTAATAAC TATGCTCAAA
13201 AATTGTGTAC CTTTAGCTTT TTAATTTGTA AAGGGGTTAA TAAGGAATAT TTGATGTATA
13261 GTGCCTTGAC TAGAGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA
13321 AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT

//

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