| 出品公司: | 爱迪基因 |
|---|---|
| 载体名称: | pCMV-dR8.2-dvpr 其它名称 pCMVdR8.2 dvpr、 pHR' CMV 8.2 deltaR |
| 质粒类型: | 哺乳动物载体；第二代慢病毒包装；双质粒包装系统 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 13380 bp |
| 5' 测序引物及序列: | CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’ |
| 3' 测序引物及序列: | -- |
| 载体标签: | 无 |
| 载体抗性: | Ampicillin |
| 筛选标记: | 无 |
| 克隆菌株: | DH5α,HB101等 |
| 宿主细胞（系）: | 包装细胞系如293T |
| 备注: |
第二代慢病毒包装载体pCMV-dR8.2-dvpr需要与信封载体 pCMV-VSVG 、pCMV-VSV-G 共同使用，使用方法见下文。用来包装第二代、第三代慢病毒表达载体。 |
| 产品目录号: | #8455 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
双质粒系统慢病毒包装质粒pCMV-dR8.2-dvpr使用方法
Day 1
1. Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scaled up if desired)
Day 2
1. Set up (use polypropylene tubes for this; polystyrene tubes DO NOT work!):
1 µg retroviral DNA encoding gene X
1 µg packaging plasmid Mix
the packaging plasmid (pCMV-dR8.2-dvpr) at a 8:1 ratio with the envelope plasmid (pCVM-VSV-G)-a total of 1µg
DME without serum to 94μL total
6 μL Fugene
Mix and wait 15 to 30 minutes at room temperature
Add to 293T cells without touching the sides of the dish (DO NOT CHANGE MEDIA)
If you are using amphotropic virus then move immediately to BL2+ in a secondary container, which has an absorbent material.
(This does not mean a couple of hours; it means Immediately!). The rest of this protocol is the same for all viruses---the BL2+ safety practices are in place if you are using amphotropic viruses
Day 3
1. Change the media to whatever media you wish to use when infecting target cells. 293T cells are easily detached so remember not to put the media directly onto to cells, but rather “run” it down the side of the dish. Remember that you will get the highest titer virus when your cells are “happy.”
2. Plate out your target cells
Day 4
1. Remove the medium from the 293T cells and use a 0.45 u syringe filter to remove any 293T cells. DO NOT use the 0.2 u filter, as it is likely to shear the envelope from your virus
making it noninfectious
Note: After filtering, the filter should be removed and placed in the biohazard bag in the hood and the syringe rinsed with bleach and decontaminated for a minimum of 20 min. It is useful to place a plastic beaker with bleach in the hood in advance
2. Add 8 to 10 μg/ml of polybrene (Hexadimethrine bromide) or protamine sulfate to the target cells
3. Carry out infection for 1 to 4 hours. Remove virus and replace with fresh media
Note: If you wish to do a second infection the following day, it is important to put fresh media on the cells and not let the virus remain on the cells overnight. The media contains huge amounts of envelope, both associated and unassociated with viral particles, which will bind all the cell surface receptors required for virus adsorption, resulting in their down-regulation. Hence, if you don’t change the media after the initial infection, very few receptors will be available for the next round of infection. In addition, very few cells tolerate the presence of high levels of the VSV-G envelope for extended periods of time (i.e. a lot of your cells may die)
Day 5 +
1. Allow the cells to recover and begin to express the virus-encoded genes. The cells usually require 48 hours for this to occur
2. Add drug if you are scoring for the presence of a vector that carries a drug resistance marker. Prior to this step it is advisable that you titrate the drug to be used for selection in order to know precisely how much to add. In addition, it is necessary to bring an extra plate of uninfected cells (often referred to as “canaries”) which will function as a positive control in the kill assay. Add drug to both plates. When your canaries are dead, you can remove the drug.
载体序列
LOCUS pCMV-dR8.2 dvpr Sequencing Result 13380 bp DNA SYN
DEFINITION pCMV-dR8.2 dvpr Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..13380
/organism="pCMV-dR8.2 dvpr Sequencing Result"
/mol_type="other DNA"
promoter 27..603
/label="CMV_immearly_promoter"
misc_feature 106..393
/label="CAG_enhancer"
misc_feature 560..580
/label="CMV_fwd_primer"
promoter 561..630
/label="CMV_promoter"
promoter 573..692
/label="CMV2_promoter"
misc_feature 604..623
/label="pCEP_fwd_primer"
misc_feature 606..630
/label="LNCX_primer"
gene 880..2382
/label="Gag_HIV (variant)"
/gene="Gag_HIV (variant)"
CDS 880..2382
/label="ORF frame 1"
/translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERF
AVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALD
KIEEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVE
EKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPV
HAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRM
YSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTIL
KALGPGATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKC
FNCGKEGHIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQ
SRPEPTAPPEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ*"
CDS 2448..5186
/label="ORF frame 3"
/translation="MNLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGP
TPVNIIGRNLLTQIGCTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEIC
TEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPH
PAGLKQKKSVTVLDVGDAYFSVPLDKDFRKYTAFTIPSINNETPGIRYQYNVLPQGWK
GSPAIFQCSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRW
GFTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQI
YAGIKVRQLCKLLRGTKALTEVVPLTEEAELELAENREILKEPVHGVYYDPSKDLIAE
IQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHTNDVKQLTEAVQKIATESIVIWGKT
PKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIIGAETFYVDG
AANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQAIHLALQDSGLEVNIVTDSQYA
LGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKLVSAGIRKVL
FLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSP
GIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTVHTD
NGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNKELKKIIGQVRDQAEHLKT
AVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRD
PVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDED*"
misc_feature 4875..4890
/label="cPPT"
/translation="MNLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGP
TPVNIIGRNLLTQIGCTLNFPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEIC
TEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPH
PAGLKQKKSVTVLDVGDAYFSVPLDKDFRKYTAFTIPSINNETPGIRYQYNVLPQGWK
GSPAIFQCSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRW
GFTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQI
YAGIKVRQLCKLLRGTKALTEVVPLTEEAELELAENREILKEPVHGVYYDPSKDLIAE
IQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHTNDVKQLTEAVQKIATESIVIWGKT
PKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIIGAETFYVDG
AANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQAIHLALQDSGLEVNIVTDSQYA
LGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKLVSAGIRKVL
FLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSP
GIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTVHTD
NGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNKELKKIIGQVRDQAEHLKT
AVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRD
PVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDED*"
CDS 5131..5709
/label="ORF frame 1"
/translation="MENRWQVMIVWQVDRMRINTWKRLVKHHMYISRKAKDWFYRHHY
ESTNPKISSEVHIPLGDAKLVITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPDL
ADQLIHLHYFDCFSESAIRNTILGRIVSPRCEYQAGHNKVGSLQYLALAALIKPKQIK
PPLPSVRKLTEDRWNKPQKTKGHRGSHTMNGH*"
CDS complement(6153..6656)
/label="ORF frame 2"
/translation="MPQTVSCNRCCCASIALSKLFCCCTIPDNNCLACTVSVIDAAPI
VLPAAPKNPRNKAPIPTALFSLCTTLLFALVGATPNGSIFTTLYLYNSLLQLSLISPP
PGLKISGRSTTDHQYPKEHGAPFPPPSPQVLILLLHSHCHCLLLFLLVYQLTCLFFFV
LIFSYIL*"
CDS 6361..7509
/label="ORF frame 1"
/translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
GSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELK
NSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL*"
misc_feature 6483..6716
/label="RRE"
/translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
GSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELK
NSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL*"
misc_feature 7239..7268
/label="HIV_Rev_NES"
/translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
GSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELK
NSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL*"
CDS 7511..8131
/label="ORF frame 2"
/translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
misc_feature 7783..7798
/label="cPPT"
/translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
misc_feature 7783..7804
/label="U3PPT"
/translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
misc_feature complement(8503..8520)
/label="Sp6_primer"
/translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
gene 8758..9021
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
/translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
misc_feature complement(8810..8829)
/label="pBRrevBam_primer"
/translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
misc_feature 9162..9184
/label="pGEX_3_primer"
/translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
promoter 9343..9371
/label="AmpR_promoter"
/translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
gene 9413..10273
/label="Ampicillin"
/gene="Ampicillin"
/translation="MGGKWSKSSVIGWPTVRERMRRAEPAADGVGAVSRDLEKHGAIT
SSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
SQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVEQEKVEEANEGE
NTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELHPEYFKNC*"
CDS 9413..10273
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 10428..11047
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(11273..11293)
/label="pBABE_3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(11279..11732)
/label="SV40_enhancer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 11291..11559
/label="SV40_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 11458..11535
/label="SV40_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 11520..11539
/label="SV40pro_F_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 12740..12755
/label="SV40_int"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 12761..12808
/label="SV40_3_splice"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS 12885..13358
/label="ORF frame 3"
/translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS
NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN
YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*"
misc_feature 12936..12956
/label="SV40 NLS"
/translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS
NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN
YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*"
misc_feature 12942..12956
/label="NLS"
/translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS
NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN
YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*"
ORIGIN
1 GGGCTGCAGG AATTCGAGCT CGCCCGACAT TGATTATTGA CTAGTTATTA ATAGTAATCA
61 ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA ACTTACGGTA
121 AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT
181 GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG
241 TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC
301 GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT
361 CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG
421 CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC
481 ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT
541 AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
601 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC GCCTGGAGAC GCCATCCACG CTGTTTTGAC
661 CTCCATAGAA GACACCGGGA CCGATCCAGC CTCCGCGGCC GGGAACGGTG CATTGGAACG
721 CGGATTCCCC GTGCCAAGAG TGACGTAAGT ACCGCCTATA GAGTCTATAG GCCCACCCCC
781 TTGGCTTCTT ATGCGACGGA TCGATCCCGT AATAAGCTTC GAGGTCCGCG GCCGCGTTGA
841 CGCGCACGGC AAGAGGCGAG GGGCGGCGAC TGGTGAGAGA TGGGTGCGAG AGCGTCAGTA
901 TTAAGCGGGG GAGAATTAGA TCGATGGGAA AAAATTCGGT TAAGGCCAGG GGGAAAGAAA
961 AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC TAGAACGATT CGCAGTTAAT
1021 CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC TGGGACAGCT ACAACCATCC
1081 CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA CAGTAGCAAC CCTCTATTGT
1141 GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT TAGACAAGAT AGAGGAAGAG
1201 CAAAACAAAA GTAAGAAAAA AGCACAGCAA GCAGCAGCTG ACACAGGACA CAGCAATCAG
1261 GTCAGCCAAA ATTACCCTAT AGTGCAGAAC ATCCAGGGGC AAATGGTACA TCAGGCCATA
1321 TCACCTAGAA CTTTAAATGC ATGGGTAAAA GTAGTAGAAG AGAAGGCTTT CAGCCCAGAA
1381 GTGATACCCA TGTTTTCAGC ATTATCAGAA GGAGCCACCC CACAAGATTT AAACACCATG
1441 CTAAACACAG TGGGGGGACA TCAAGCAGCC ATGCAAATGT TAAAAGAGAC CATCAATGAG
1501 GAAGCTGCAG AATGGGATAG AGTGCATCCA GTGCATGCAG GGCCTATTGC ACCAGGCCAG
1561 ATGAGAGAAC CAAGGGGAAG TGACATAGCA GGAACTACTA GTACCCTTCA GGAACAAATA
1621 GGATGGATGA CACATAATCC ACCTATCCCA GTAGGAGAAA TCTATAAAAG ATGGATAATC
1681 CTGGGATTAA ATAAAATAGT AAGAATGTAT AGCCCTACCA GCATTCTGGA CATAAGACAA
1741 GGACCAAAGG AACCCTTTAG AGACTATGTA GACCGATTCT ATAAAACTCT AAGAGCCGAG
1801 CAAGCTTCAC AAGAGGTAAA AAATTGGATG ACAGAAACCT TGTTGGTCCA AAATGCGAAC
1861 CCAGATTGTA AGACTATTTT AAAAGCATTG GGACCAGGAG CGACACTAGA AGAAATGATG
1921 ACAGCATGTC AGGGAGTGGG GGGACCCGGC CATAAAGCAA GAGTTTTGGC TGAAGCAATG
1981 AGCCAAGTAA CAAATCCAGC TACCATAATG ATACAGAAAG GCAATTTTAG GAACCAAAGA
2041 AAGACTGTTA AGTGTTTCAA TTGTGGCAAA GAAGGGCACA TAGCCAAAAA TTGCAGGGCC
2101 CCTAGGAAAA AGGGCTGTTG GAAATGTGGA AAGGAAGGAC ACCAAATGAA AGATTGTACT
2161 GAGAGACAGG CTAATTTTTT AGGGAAGATC TGGCCTTCCC ACAAGGGAAG GCCAGGGAAT
2221 TTTCTTCAGA GCAGACCAGA GCCAACAGCC CCACCAGAAG AGAGCTTCAG GTTTGGGGAA
2281 GAGACAACAA CTCCCTCTCA GAAGCAGGAG CCGATAGACA AGGAACTGTA TCCTTTAGCT
2341 TCCCTCAGAT CACTCTTTGG CAGCGACCCC TCGTCACAAT AAAGATAGGG GGGCAATTAA
2401 AGGAAGCTCT ATTAGATACA GGAGCAGATG ATACAGTATT AGAAGAAATG AATTTGCCAG
2461 GAAGATGGAA ACCAAAAATG ATAGGGGGAA TTGGAGGTTT TATCAAAGTA AGACAGTATG
2521 ATCAGATACT CATAGAAATC TGCGGACATA AAGCTATAGG TACAGTATTA GTAGGACCTA
2581 CACCTGTCAA CATAATTGGA AGAAATCTGT TGACTCAGAT TGGCTGCACT TTAAATTTTC
2641 CCATTAGTCC TATTGAGACT GTACCAGTAA AATTAAAGCC AGGAATGGAT GGCCCAAAAG
2701 TTAAACAATG GCCATTGACA GAAGAAAAAA TAAAAGCATT AGTAGAAATT TGTACAGAAA
2761 TGGAAAAGGA AGGAAAAATT TCAAAAATTG GGCCTGAAAA TCCATACAAT ACTCCAGTAT
2821 TTGCCATAAA GAAAAAAGAC AGTACTAAAT GGAGAAAATT AGTAGATTTC AGAGAACTTA
2881 ATAAGAGAAC TCAAGATTTC TGGGAAGTTC AATTAGGAAT ACCACATCCT GCAGGGTTAA
2941 AACAGAAAAA ATCAGTAACA GTACTGGATG TGGGCGATGC ATATTTTTCA GTTCCCTTAG
3001 ATAAAGACTT CAGGAAGTAT ACTGCATTTA CCATACCTAG TATAAACAAT GAGACACCAG
3061 GGATTAGATA TCAGTACAAT GTGCTTCCAC AGGGATGGAA AGGATCACCA GCAATATTCC
3121 AGTGTAGCAT GACAAAAATC TTAGAGCCTT TTAGAAAACA AAATCCAGAC ATAGTCATCT
3181 ATCAATACAT GGATGATTTG TATGTAGGAT CTGACTTAGA AATAGGGCAG CATAGAACAA
3241 AAATAGAGGA ACTGAGACAA CATCTGTTGA GGTGGGGATT TACCACACCA GACAAAAAAC
3301 ATCAGAAAGA ACCTCCATTC CTTTGGATGG GTTATGAACT CCATCCTGAT AAATGGACAG
3361 TACAGCCTAT AGTGCTGCCA GAAAAGGACA GCTGGACTGT CAATGACATA CAGAAATTAG
3421 TGGGAAAATT GAATTGGGCA AGTCAGATTT ATGCAGGGAT TAAAGTAAGG CAATTATGTA
3481 AACTTCTTAG GGGAACCAAA GCACTAACAG AAGTAGTACC ACTAACAGAA GAAGCAGAGC
3541 TAGAACTGGC AGAAAACAGG GAGATTCTAA AAGAACCGGT ACATGGAGTG TATTATGACC
3601 CATCAAAAGA CTTAATAGCA GAAATACAGA AGCAGGGGCA AGGCCAATGG ACATATCAAA
3661 TTTATCAAGA GCCATTTAAA AATCTGAAAA CAGGAAAGTA TGCAAGAATG AAGGGTGCCC
3721 ACACTAATGA TGTGAAACAA TTAACAGAGG CAGTACAAAA AATAGCCACA GAAAGCATAG
3781 TAATATGGGG AAAGACTCCT AAATTTAAAT TACCCATACA AAAGGAAACA TGGGAAGCAT
3841 GGTGGACAGA GTATTGGCAA GCCACCTGGA TTCCTGAGTG GGAGTTTGTC AATACCCCTC
3901 CCTTAGTGAA GTTATGGTAC CAGTTAGAGA AAGAACCCAT AATAGGAGCA GAAACTTTCT
3961 ATGTAGATGG GGCAGCCAAT AGGGAAACTA AATTAGGAAA AGCAGGATAT GTAACTGACA
4021 GAGGAAGACA AAAAGTTGTC CCCCTAACGG ACACAACAAA TCAGAAGACT GAGTTACAAG
4081 CAATTCATCT AGCTTTGCAG GATTCGGGAT TAGAAGTAAA CATAGTGACA GACTCACAAT
4141 ATGCATTGGG AATCATTCAA GCACAACCAG ATAAGAGTGA ATCAGAGTTA GTCAGTCAAA
4201 TAATAGAGCA GTTAATAAAA AAGGAAAAAG TCTACCTGGC ATGGGTACCA GCACACAAAG
4261 GAATTGGAGG AAATGAACAA GTAGATAAAT TGGTCAGTGC TGGAATCAGG AAAGTACTAT
4321 TTTTAGATGG AATAGATAAG GCCCAAGAAG AACATGAGAA ATATCACAGT AATTGGAGAG
4381 CAATGGCTAG TGATTTTAAC CTACCACCTG TAGTAGCAAA AGAAATAGTA GCCAGCTGTG
4441 ATAAATGTCA GCTAAAAGGG GAAGCCATGC ATGGACAAGT AGACTGTAGC CCAGGAATAT
4501 GGCAGCTAGA TTGTACACAT TTAGAAGGAA AAGTTATCTT GGTAGCAGTT CATGTAGCCA
4561 GTGGATATAT AGAAGCAGAA GTAATTCCAG CAGAGACAGG GCAAGAAACA GCATACTTCC
4621 TCTTAAAATT AGCAGGAAGA TGGCCAGTAA AAACAGTACA TACAGACAAT GGCAGCAATT
4681 TCACCAGTAC TACAGTTAAG GCCGCCTGTT GGTGGGCGGG GATCAAGCAG GAATTTGGCA
4741 TTCCCTACAA TCCCCAAAGT CAAGGAGTAA TAGAATCTAT GAATAAAGAA TTAAAGAAAA
4801 TTATAGGACA GGTAAGAGAT CAGGCTGAAC ATCTTAAGAC AGCAGTACAA ATGGCAGTAT
4861 TCATCCACAA TTTTAAAAGA AAAGGGGGGA TTGGGGGGTA CAGTGCAGGG GAAAGAATAG
4921 TAGACATAAT AGCAACAGAC ATACAAACTA AAGAATTACA AAAACAAATT ACAAAAATTC
4981 AAAATTTTCG GGTTTATTAC AGGGACAGCA GAGATCCAGT TTGGAAAGGA CCAGCAAAGC
5041 TCCTCTGGAA AGGTGAAGGG GCAGTAGTAA TACAAGATAA TAGTGACATA AAAGTAGTGC
5101 CAAGAAGAAA AGCAAAGATC ATCAGGGATT ATGGAAAACA GATGGCAGGT GATGATTGTG
5161 TGGCAAGTAG ACAGGATGAG GATTAACACA TGGAAAAGAT TAGTAAAACA CCATATGTAT
5221 ATTTCAAGGA AAGCTAAGGA CTGGTTTTAT AGACATCACT ATGAAAGTAC TAATCCAAAA
5281 ATAAGTTCAG AAGTACACAT CCCACTAGGG GATGCTAAAT TAGTAATAAC AACATATTGG
5341 GGTCTGCATA CAGGAGAAAG AGACTGGCAT TTGGGTCAGG GAGTCTCCAT AGAATGGAGG
5401 AAAAAGAGAT ATAGCACACA AGTAGACCCT GACCTAGCAG ACCAACTAAT TCATCTGCAC
5461 TATTTTGATT GTTTTTCAGA ATCTGCTATA AGAAATACCA TATTAGGACG TATAGTTAGT
5521 CCTAGGTGTG AATATCAAGC AGGACATAAC AAGGTAGGAT CTCTACAGTA CTTGGCACTA
5581 GCAGCATTAA TAAAACCAAA ACAGATAAAG CCACCTTTGC CTAGTGTTAG GAAACTGACA
5641 GAGGACAGAT GGAACAAGCC CCAGAAGACC AAGGGCCACA GAGGGAGCCA TACAATGAAT
5701 GGACACTAGA GCTTTAGTAG CCCGAACCCG GGGCGGACCG AAGTGGAAGC CATAATAAGA
5761 ATTCTGCAAC AACTGCTGTT TATCCATTTC AGAATTGGGT GTCGACATAG CAGAATAGGC
5821 GTTACTCGAC AGAGGAGAGC AAGAAATGGA GCCAGTAGAT CCTAGACTAG AGCCCTGGAA
5881 GCATCCAGGA AGTCAGCCTA AAACTGCTTG TACCAATTGC TATTGTAAAA AGTGTTGCTT
5941 TCATTGCCAA GTTTGTTTCA TGACAAAAGC CTTAGGCATC TCCTATGGCA GGAAGAAGCG
6001 GAGACAGCGA CGAAGAGCTC ATCAGAACAG TCAGACTCAT CAAGCTTCTC TATCAAAGCA
6061 GTAAGTAGTA CATGTAATGC AACCTATAAT AGTAGCAATA GTAGCATTAG TAGTAGCAAT
6121 AATAATAGCA ATAGTTGTGT GGTCCATAGT AATCATAGAA TATAGGAAAA TATTAAGACA
6181 AAGAAAAATA GACAGGTTAA TTGATAGACT AATAGAAAGA GCAGAAGACA GTGGCAATGA
6241 GAGTGAAGGA GAAGTATCAG CACTTGTGGA GATGGGGGTG GAAATGGGGC ACCATGCTCC
6301 TTGGGATATT GATGATCTGT AGTTGAGCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT
6361 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA
6421 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA
6481 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA
6541 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT
6601 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG
6661 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG
6721 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG
6781 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA
6841 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA
6901 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC
6961 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT
7021 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA
7081 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA
7141 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCCTTGGCA
7201 CTTATCTGGG ACGATCTGCG GAGCCTGTGC CTCTTCAGCT ACCACCGCTT GAGAGACTTA
7261 CTCTTGATTG TAACGAGGAT TGTGGAACTT CTGGGACGCA GGGGGTGGGA AGCCCTCAAA
7321 TATTGGTGGA ATCTCCTACA ATATTGGAGT CAGGAGCTAA AGAATAGTGC TGTTAGCTTG
7381 CTCAATGCCA CAGCCATAGC AGTAGCTGAG GGGACAGATA GGGTTATAGA AGTAGTACAA
7441 GGAGCTTGTA GAGCTATTCG CCACATACCT AGAAGAATAA GACAGGGCTT GGAAAGGATT
7501 TTGCTATAAG ATGGGTGGCA AGTGGTCAAA AAGTAGTGTG ATTGGATGGC CTACTGTAAG
7561 GGAAAGAATG AGACGAGCTG AGCCAGCAGC AGATGGGGTG GGAGCAGTAT CTCGAGACCT
7621 AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT ACCAATGCTG ATTGTGCCTG
7681 GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC ACACCTCAGG TACCTTTAAG
7741 ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT
7801 GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATATCCTT GATCTGTGGA TCTACCACAC
7861 ACAAGGCTAC TTCCCTGATT GGCAGAACTA CACACCAGGG CCAGGGATCA GATATCCACT
7921 GACCTTTGGA TGGTGCTACA AGCTAGTACC AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA
7981 TGAAGGAGAG AACACCCGCT TGTTACACCC TGTGAGCCTG CATGGGATGG ATGACCCGGA
8041 GAGAGAAGTA TTAGAGTGGA GGTTTGACAG CCGCCTAGCA TTTCATCACA TGGCCCGAGA
8101 GCTGCATCCG GAGTACTTCA AGAACTGCTG AGCGGCCGCC CCGGTGACCT TCAGACCTTG
8161 GCACTGGAGG TGGCCCGGCA GAAGCGCGGC ATCGTGGATC AGTGCTGCAC CAGCATCTGC
8221 TCTCTCTACC AACTGGAGAA CTACTGCAAC TAGGCCCACC ACTACCCTGT CCACCCCTCT
8281 GCAATGAATA AAACCTTTGA AAGAGCACTA CAAGTTGTGT GTACATGCGT GCATGTGCAT
8341 ATGTGGTGCG GGGGGAACAT GAGTGGGGCT GGCTGGAGTG GCGATGATAA GCTGTCAAAC
8401 ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA
8461 TGATAATAAT GGTTTCTTAG TCTAGAATTA ATTCCGTGTA TTCTATAGTG TCACCTAAAT
8521 CGTATGTGTA TGATACATAA GGTTATGTAT TAATTGTAGC CGCGTTCTAA CGACAATATG
8581 TACAAGCCTA ATTGTGTAGC ATCTGGCTTA CTGAAGCAGA CCCTATCATC TCTCTCGTAA
8641 ACTGCCGTCA GAGTCGGTTT GGTTGGACGA ACCTTCTGAG TTTCTGGTAA CGCCGTCCCG
8701 CACCCGGAAA TGGTCAGCGA ACCAATCAGC AGGGTCATCG CTAGCCAGAT CCTCTACGCC
8761 GGACGCATCG TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC
8821 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC
8881 GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA
8941 CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG
9001 CAGGAGTCGC ATAAGGGAGA GCGTCGAATG GTGCACTCTC AGTACAATCT GCTCTGATGC
9061 CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG
9121 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC Tccgggagct gcatgtgtca
9181 gaggttttca ccgtcatcac cgaaacgcgc gagACGAAAG GGCCTCGTGA TACGCCTATT
9241 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG
9301 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
9361 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
9421 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
9481 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
9541 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
9601 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
9661 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
9721 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
9781 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
9841 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
9901 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
9961 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
10021 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
10081 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT
10141 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
10201 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
10261 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
10321 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
10381 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
10441 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
10501 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
10561 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
10621 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
10681 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
10741 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
10801 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
10861 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
10921 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
10981 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
11041 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC
11101 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC
11161 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC
11221 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GTGGAATGTG
11281 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG
11341 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT
11401 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC
11461 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT
11521 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC
11581 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTTGGACACA AGACAGGCTT GCGAGATATG
11641 TTTGAGAATA CCACTTTATC CCGCGTCAGG GAGAGGCAGT GCGTAAAAAG ACGCGGACTC
11701 ATGTGAAATA CTGGTTTTTA GTGCGCCAGA TCTCTATAAT CTCGCGCAAC CTATTTTCCC
11761 CTCGAACACT TTTTAAGCCG TAGATAAACA GGCTGGGACA CTTCACATGA GCGAAAAATA
11821 CATCGTCACC TGGGACATGT TGCAGATCCA TGCACGTAAA CTCGCAAGCC GACTGATGCC
11881 TTCTGAACAA TGGAAAGGCA TTATTGCCGT AAGCCGTGGC GGTCTGTACC GGGTGCGTTA
11941 CTGGCGCGTG AACTGGGTAT TCGTCATGTC GATACCGTTT GTATTTCCAG CTACGATCAC
12001 GACAACCAGC GCGAGCTTAA AGTGCTGAAA CGCGCAGAAG GCGATGGCGA AGGCTTCATC
12061 GTTATTGATG ACCTGGTGGA TACCGGTGGT ACTGCGGTTG CGATTCGTGA AATGTATCCA
12121 AAAGCGCACT TTGTCACCAT CTTCGCAAAA CCGGCTGGTC GTCCGCTGGT TGATGACTAT
12181 GTTGTTGATA TCCCGCAAGA TACCTGGATT GAACAGCCGT GGGATATGGG CGTCGTATTC
12241 GTCCCGCCAA TCTCCGGTCG CTAATCTTTT CAACGCCTGG CACTGCCGGG CGTTGTTCTT
12301 TTTAACTTCA GGCGGGTTAC AATAGTTTCC AGTAAGTATT CTGGAGGCTG CATCCATGAC
12361 ACAGGCAAAC CTGAGCGAAA CCCTGTTCAA ACCCCGCTTT AAACATCCTG AAACCTCGAC
12421 GCTAGTCCGC CGCTTTAATC ACGGCGCACA ACCGCCTGTG CAGTCGGCCC TTGATGGTAA
12481 AACCATCCCT CACTGGTATC GCATGATTAA CCGTCTGATG TGGATCTGGC GCGGCATTGA
12541 CCCACGCGAA ATCCTCGACG TCCAGGCACG TATTGTGATG AGCGATGCCG AACGTACCGA
12601 CGATGATTTA TACGATACGG TGATTGGCTA CCGTGGCGGC AACTGGATTT ATGAGTGGGC
12661 CCCGGATCTT TGTGAAGGAA CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA
12721 CAGAGATTTA AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
12781 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA TGGGAGCAGT
12841 GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA AGAAATGCCA TCTAGTGATG
12901 ATGAGGCTAC TGCTGACTCT CAACATTCTA CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG
12961 ACCCCAAGGA CTTTCCTTCA GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA
13021 GAACTCTTGC TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
13081 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT AATCATAACA
13141 TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC TATTAATAAC TATGCTCAAA
13201 AATTGTGTAC CTTTAGCTTT TTAATTTGTA AAGGGGTTAA TAAGGAATAT TTGATGTATA
13261 GTGCCTTGAC TAGAGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA
13321 AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
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