| 质粒名称: | pDONR223-LRPPRC |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 23521 |
| 存储实验室: | William Hahn, David Root |
|
载体骨架基本信息 |
|
| 载体名称: | pDONR223 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 壮观霉素 |
| 空载体大小: | 5005 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | Gateway Donor vector |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LRPPRC,leucine-rich PPR-motif containing;LRPPRC |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4185 bp |
| 基因突变: | G246A；删除E579 |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13pUC-fwd |
| 3' 测序引物 | M13rev |
|
文献引用方法 |
|
| 材料和方法部分: | pDONR223-LRPPRC was a gift from William Hahn & David Root (Addgene plasmid # 23521 ; http://n2t.net/addgene:23521 ; RRID:Addgene_23521) |
| 参考文献部分: | COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA, Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM, Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA. Nature. 2010 Nov 24. ():. 10.1038/nature09627 PubMed 21107320 |
买家导航
pDONR223LRPPRC载体基本信息
pDONR223LRPPRC载体图谱质粒图谱和多克隆位点信息
pDONR223LRPPRC载体简介
pDONR223LRPPRC载体序列
LOCUS pDONR223-LRPPRC 6972 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pDONR223-LRPPRC
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6972)
AUTHORS Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G,
Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao
JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL,
Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
TITLE COT drives resistance to RAF inhibition through MAP kinase pathway
reactivation.
JOURNAL Nature. 2010 Nov 24. ():.
PUBMED 21107320
REFERENCE 2 (bases 1 to 6972)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6972
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 149..176
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 268..354
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 403..425
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 417..434
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 418..434
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 450..549
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
protein_bind complement(4732..4831)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(4848..4867)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(4849..4867)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4872..4888)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4872..4888)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 5319..6110
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 6203..6791
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6692..6711
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6945..6962
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
121 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
181 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
241 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
301 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
361 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
421 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
481 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
541 aagttggcac catggcagcc ctgctgagat ccgcgcgttg gttgctgcgt gccggggcgg
601 ccccgcgcct cccgctctcc ctgcgcctcc tccctggcgg cccgggccgg ctgcatgccg
661 cctcctatct gcccgccgct cgcgccgggc ccgtggccgg aggactactg agcccagcca
721 ggctgtatgc cattgctgcc aaagaaaaag atattcaaga ggagtccact ttttcttcta
781 ggaagatttc caatcaattt gattgggctc taatgagact agatctttct gttcgaagaa
841 ctggccgcat tccaaagaag cttctacaaa aagtttttaa tgatacctgc cgctcaggtg
901 gcctaggtgg tagtcatgcc ttgcttctac tacgtagttg tggttctctc ttgcctgaac
961 taaagcttga agagagaaca gaatttgctc ataggatatg ggacacactt cagaaattag
1021 gtgctgtgta tgatgtgagt cactataatg ctttacttaa agtctatctt caaaatgaat
1081 ataaattctc accaactgat ttcctggcaa aaatggagga agcaaacatt caaccaaatc
1141 gagtgacata ccagagattg attgcttctt attgtaatgt aggagatatt gaaggtgcca
1201 gcaagattct tggatttatg aaaactaagg atctcccagt tacagaggca gtattcagtg
1261 cccttgtgac agggcatgcc agagctggtg atatggagaa tgcagaaaac attctcacag
1321 tgatgagaga tgccggaatt gagcctggtc cagacacata cctcgcatta ttgaatgcat
1381 atgctgagaa gggcgacatt gaccatgtta agcagactct ggagaaggtg gagaagtccg
1441 agcttcacct tatggaccgt gatttactgc aaattatttt tagcttcagt aaagctgggt
1501 atcctcagta tgtctcagaa attttggaaa aagttacatg tgaaagaaga tatattccag
1561 atgcaatgaa cctcatttta cttttagtca ctgaaaaatt ggaagatgta gcgttgcaaa
1621 ttttactagc atgccccgta tcaaaggaag atggcccaag tgtctttggc agtttctttt
1681 tacaacactg tgtgactatg aatacgcctg tggagaagct aacagactac tgtaagaagt
1741 taaaggaagt ccagatgcac tcctttcctc tgcagttcac cctccattgt gctttactcg
1801 ccaataaaac tgatttggca aaagccttaa tgaaggctgt gaaggaggaa ggttttccta
1861 tcagacctca ctatttctgg ccattgctag ttggacgtcg gaaggaaaaa aatgttcaag
1921 gtataattga aatcctcaaa ggaatgcaag aattgggagt acatcctgat caggaaacat
1981 atacagatta tgtgattcca tgctttgata gtgtaaactc agcacgagcc attttgcagg
2041 aaaatggatg tctgtctgat agtgatatgt tttctcaagc tggattgaga agtgaagcag
2101 caaatgggaa cttagacttt gtattatcat ttttgaaatc aaatacattg cccatctcgc
2161 tgcagtctat aagaagtagc ctactgctag gcttcaggag gtctatgaat ataaatcttt
2221 ggagcgagat aacagaattg ttgtacaagg atggacgtta ttgccaggag cctcgaggac
2281 cgacggctgt tggctatttt ctttataact tgattgacag catgagtgac tcagaggtac
2341 aggccaagga ggagcatttg agacaatact tccatcagct ggagaagatg aatgtaaaaa
2401 ttcctgaaaa tatctacaga ggcattcgta atctcctgga aagctaccat gttcctgaat
2461 tgattaagga tgctcacttg ttggttgaga gtaagaattt agactttcaa aaaactgtgc
2521 aacttacatc atctgaattg gagtccacac ttgaaacact aaaagctgaa aatcaaccta
2581 taagagatgt cctaaagcaa ctcatattag tgctttgttc agaagagaat atgcaaaaag
2641 cccttgaatt gaaagcaaaa tatgaatccg acatggttac tggtggctat gcagctttaa
2701 taaatttatg ctgtcgacat gataaagtag aagatgcctt gaacttgaaa gaagaatttg
2761 accgcttaga ttcatctgct gtccttgaca ccggcaagta tgtaggcctt gtaagagtat
2821 tggcaaagca tggcaagctc caagatgcta ttaacattct gaaggagatg aaagagaagg
2881 atgttcttat caaagataca acagccttgt cctttttcca catgctaaat ggcgcagctt
2941 taagaggtga aattgaaaca gtaaaacagt tgcatgaagc catcgtgact ctagggttag
3001 cagaaccatc caccaacata agtttcccat tggtcactgt acacttggaa aagggcgacc
3061 tatctactgc tcttgaggtc gccattgact gctatgaaaa gtataaagta ttaccaagga
3121 ttcatgatgt cttgtgtaaa ctggtagaga aaggcgagac tgatctaatt cagaaagcaa
3181 tggactttgt gagccaagaa caaggtgaaa tggtgatgct ctatgatctc ttctttgcct
3241 tcctacaaac aggaaattac aaagaggcca agaagatcat tgagactcca gggattagag
3301 ctcgatctgc aaggcttcag tggttttgtg acagatgtgt tgcaaataat caggttgaaa
3361 ctctggaaaa attagtggag ctgacacaga agctatttga atgtgataga gaccagatgt
3421 actacaatct gctaaaactg tataaaataa acggtgactg gcaaagagct gatgcagtct
3481 ggaataaaat ccaagaagaa aatgttattc ctcgtgaaaa gacattaaga ttattagcag
3541 aaatccttag agagggtaac caggaagttc cgtttgacgt acctgagttg tggtatgaag
3601 atgaaaaaca ttccctgaat tcttcgtcag cctcaaccac agaacctgat ttccagaaag
3661 atatattgat tgcctgccga ttgaaccaaa aaaaaggggc atatgatatt ttcctgaatg
3721 caaaagagca aaacattgtg tttaatgctg aaacctacag caatctcatt aaattactga
3781 tgtcagaaga ttattttaca caagcaatgg aagtgaaagc attcgcggag acccacatca
3841 agggcttcac actgaacgat gctgccaaca gccgcctcat cataacgcaa gttaggcggg
3901 attatttgaa agaggctgtg acaacactga aaacagtatt ggatcagcag cagacccctt
3961 ctaggttagc agtgacccgt gtcatccagg cattggccat gaagggtgat gttgaaaaca
4021 tagaagtagt tcagaagatg ttaaatggac tcgaagactc cattggactt tcaaaaatgg
4081 ttttcatcaa taacattgct ttggctcaaa taaagaataa taacatagat gccgcaatag
4141 aaaacattga aaatatgctt acttcagaga ataaagtcat tgaaccccaa tacttcggct
4201 tggcatactt attcagaaaa gtaatagagg agcagttgga accagcagtt gaaaagataa
4261 gcatcatggc ggagagattg gccaatcagt ttgcaattta taaacctgtc actgattttt
4321 tccttcaact tgtggatgca ggcaaggtgg atgatgccag agctctccta cagagatgtg
4381 gtgcaattgc tgaacaaacc ccgattttgt tgttgttcct ccttaggaat tctaggaaac
4441 aaggaaaggc atcaactgtg aaatctgtgt tagaattgat tcctgaatta aatgaaaagg
4501 aagaagcata caattccctc atgaaaagct atgtctcaga gaaagatgtc acatctgcta
4561 aagcactgta tgaacatttg actgcaaaga atacaaaatt ggatgatctg tttctaaagc
4621 gttacgcatc tttgctgaag tatgctggag agcctgtccc tttcattgaa ccccctgaaa
4681 gctttgaatt ttatgcacag cagctaagaa aattgaggga aaactcttct ttgccaactt
4741 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
4801 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
4861 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
4921 gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa
4981 atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
5041 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
5101 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
5161 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
5221 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
5281 cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg
5341 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
5401 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
5461 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
5521 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
5581 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
5641 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
5701 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
5761 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
5821 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
5881 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
5941 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
6001 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
6061 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc
6121 acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
6181 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
6241 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
6301 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
6361 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
6421 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
6481 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
6541 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
6601 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
6661 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
6721 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
6781 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
6841 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
6901 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
6961 aggaagcgga ag
//
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