出品公司: | Euroscarf |
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载体名称: | pFA6a-natMX6 |
质粒类型: | 酵母表达载体,酵母基因编辑质粒 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | AgTEF |
载体大小: | 3.7 kb |
5’测序引物: | -- |
3’测序引物: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
克隆菌株: | DH5 alpha; 等 |
宿主细胞(系): | 酵母菌 |
产品目录号: | P30437 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
备注: | pFA6a-natMX6是酵母菌基因编辑载体。 |
参考文献: | Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J , Carr AM . Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe. Yeast. 2005; 22, 1013-9. |
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pFA6a-natMX6载体质粒基本信息
pFA6a-natMX6质粒图谱载体图谱和pFA6a-natMX6载体序列质粒序列多克隆位点信息
pFA6a-natMX6质粒载体简介
pFA6a-natMX6质粒序列载体序列
LOCUS pFA6a-natMX6 3704 bp DNA circular SYN 07-MAR-2024 DEFINITION Plasmid for yeast gene deletion using the natMX6 selectable marker conferring nourseothricin resistance. ACCESSION . VERSION . KEYWORDS pFA6a-natMX6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3704) AUTHORS Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J, Carr AM. TITLE Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe. JOURNAL Yeast 2005;22:1013-9. PUBMED 16200533 REFERENCE 2 (bases 1 to 3704) AUTHORS EUROSCARF TITLE Direct Submission JOURNAL Exported Jul 14, 2024 from SnapGene 6.0.2 https://www.biofeng.com COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; date: "2005"; volume: "22"; pages: "1013-9" FEATURES Location/Qualifiers source 1..3704 /lab_host="Schizosaccharomyces pombe" /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 40..59 /label=F1 /note="Forward primer for gene deletion. This primer includes a BamHI recognition sequence. A gene-specific sequence should be added at the 5' end of the primer." /note="color: black; sequence: CGGATCCCCGGGTTAATTAA" gene 113..1235 /label=natMX6 /note="yeast selectable marker conferring nourseothricin resistance" /note="color: #ff7f50; direction: RIGHT" promoter 113..456 /label=TEF promoter /note="Ashbya gossypii TEF promoter" /note="color: #ffffff; direction: RIGHT" CDS 457..1032 /codon_start=1 /gene="Streptomyces noursei nat1" /product="nourseothricin acetyltransferase" /label=NrsR /note="confers resistance to nourseothricin" /note="color: #ccffcc" /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVISYSAWNR RLTVEDIEVAPEHRGHGVGRALMGLATEFAGERGAGHLWLEVTNVNAPAIHAYRRMGFT LCGLDTALYDGTASDGERQALYMSMPCP" terminator 1038..1235 /label=TEF terminator /note="Ashbya gossypii TEF terminator" /note="color: #ffffff" primer_bind complement(1266..1285) /label=R1 /note="Reverse primer for gene deletion or C-terminal tagging. This primer includes an EcoRI recognition sequence. A gene-specific sequence should be added at the 5' end of the primer." /note="color: black; sequence: GAATTCGAGCTCGTTTAAAC" promoter complement(1340..1358) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" /note="color: #ffffff; direction: LEFT" rep_origin complement(1616..2204) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(2375..3235) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 2375 .. 3166 / #ccffcc 2: 3167 .. 3235 / #ccffcc / signal sequence Cleavage site after base 3166" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3236..3340) /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: LEFT" promoter 3686..3704 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" /note="color: #ffffff; direction: RIGHT" ORIGIN 1 acgcggccgc cagctgaagc ttcgtacgct gcaggtcgac ggatccccgg gttaattaag 61 gcgcgccaga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga 121 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg 181 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga 241 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa 301 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa 361 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg 421 atacagttct cacatcacat ccgaacataa acaaccatgg gtaccactct tgacgacacg 481 gcttaccggt accgcaccag tgtcccgggg gacgccgagg ccatcgaggc actggatggg 541 tccttcacca ccgacaccgt cttccgcgtc accgccaccg gggacggctt caccctgcgg 601 gaggtgccgg tggacccgcc cctgaccaag gtgttccccg acgacgaatc ggacgacgaa 661 tcggacgacg gggaggacgg cgacccggac tcccggacgt tcgtcgcgta cggggacgac 721 ggcgacctgg cgggcttcgt ggtcatctcg tactcggcgt ggaaccgccg gctgaccgtc 781 gaggacatcg aggtcgcccc ggagcaccgg gggcacgggg tcgggcgcgc gttgatgggg 841 ctcgcgacgg agttcgccgg cgagcggggc gccgggcacc tctggctgga ggtcaccaac 901 gtcaacgcac cggcgatcca cgcgtaccgg cggatggggt tcaccctctg cggcctggac 961 accgccctgt acgacggcac cgcctcggac ggcgagcggc aggcgctcta catgagcatg 1021 ccctgcccct aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg 1081 tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt 1141 tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat 1201 gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc 1261 atccagttta aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcgg 1321 ccgcggatct gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc 1381 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 1441 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 1501 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 1561 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 1621 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 1681 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 1741 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 1801 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 1861 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 1921 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 1981 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 2041 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 2101 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 2161 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 2221 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 2281 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 2341 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 2401 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 2461 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 2521 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 2581 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 2641 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 2701 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 2761 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 2821 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 2881 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 2941 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 3001 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 3061 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 3121 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 3181 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 3241 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 3301 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 3361 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 3421 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 3481 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 3541 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 3601 ttgtactgag agtgcaccat atggacatat tgtcgttaga acgcggctac aattaataca 3661 taaccttatg tatcatacac atacgattta ggtgacacta taga //