出品公司: | ATCC |
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载体名称: | pRS303 |
质粒类型: | 酵母表达载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | T3 |
载体大小: | 4.4 kb |
5’测序引物: | M13-F:TGTAAAACGACGGCCAGT |
3’测序引物: | M13-R:CAGGAAACAGCTATGACC |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | 组氨酸筛选 |
克隆菌株: | DH5 alpha; 等 |
宿主细胞(系): | 酿酒酵母 |
产品目录号: | 77138 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
备注: | pRS303载体是酿酒酵母表达载体。 |
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pRS303载体质粒基本信息
pRS303质粒图谱载体图谱和pRS303载体序列质粒序列多克隆位点信息
pRS303质粒载体简介
pRS303质粒序列载体序列
LOCUS pRS303 4443 bp DNA circular SYN 07-MAR-2024 DEFINITION Yeast integrative vector with a HIS3 marker and an MCS derived from pBLUESCRIPT. ACCESSION U03435 VERSION . KEYWORDS pRS303 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4443) AUTHORS Sikorski RS, Hieter P. TITLE A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. JOURNAL Genetics 1989;122:19-27. PUBMED 2659436 REFERENCE 2 (bases 1 to 4443) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 14, 2024 from SnapGene 6.0.2 https://www.biofeng.com COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; date: "1989"; volume: "122"; pages: "19-27" FEATURES Location/Qualifiers source 1..4443 /lab_host="Saccharomyces cerevisiae" /mol_type="other DNA" /organism="synthetic DNA construct" promoter 315..502 /gene="S. cerevisiae HIS3" /label=HIS3 promoter /note="color: #ffffff; direction: RIGHT" CDS 503..1162 /codon_start=1 /gene="S. cerevisiae HIS3" /product="imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis" /label=HIS3 /note="yeast auxotrophic marker" /note="color: #ff7f50" /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM" rep_origin complement(1423..1878) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /note="color: #ffff00" CDS complement(1661..2236) /codon_start=1 /gene="lacZ fragment" /product="fragment of beta-galactosidase" /label=lacZ' /note="color: #993366" /translation="MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCSPGDPLV LERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART DRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAG FPRQALNRGLPLGFRFSALRHLDPKKLD" primer_bind 2023..2039 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" /note="color: #a020f0; direction: RIGHT" promoter 2046..2064 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" /note="color: #ffffff; direction: RIGHT" misc_feature 2073..2180 /label=MCS /note="pBluescript multiple cloning site" /note="color: #99ccff" promoter complement(2193..2211) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" /note="color: #ffffff; direction: LEFT" primer_bind complement(2232..2248) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" /note="color: #a020f0; direction: LEFT" protein_bind 2256..2272 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /note="color: #31849b" promoter complement(2280..2310) /label=lac promoter /note="promoter for the E. coli lac operon" /note="This reverse directional feature has 3 segments: 1: 2280 .. 2286 / #ffffff / -10 2: 2287 .. 2304 / #ffffff 3: 2305 .. 2310 / #ffffff / -35" rep_origin complement(2634..3222) /direction=LEFT /label=ori /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(3393..4253) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 3393 .. 4184 / #ccffcc 2: 4185 .. 4253 / #ccffcc / signal sequence Cleavage site after base 4184" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4254..4358) /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: LEFT" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga 241 acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt 301 actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt 361 ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa 421 tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca 481 ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa 541 tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc 601 gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat 661 taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc 721 cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac 781 tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctactgg cgcgtggagt 841 aaaaaggttt ggatcaggat ttgcgccttt ggatgaggca ctttccagag cggtggtaga 901 tctttcgaac aggccgtacg cagttgtcga acttggtttg caaagggaga aagtaggaga 961 tctctcttgc gagatgatcc cgcattttct tgaaagcttt gcagaggcta gcagaattac 1021 cctccacgtt gattgtctgc gaggcaagaa tgatcatcac cgtagtgaga gtgcgttcaa 1081 ggctcttgcg gttgccataa gagaagccac ctcgcccaat ggtaccaacg atgttccctc 1141 caccaaaggt gttcttatgt agtgacaccg attatttaaa gctgcagcat acgatatata 1201 tacatgtgta tatatgtata cctatgaatg tcagtaagta tgtatacgaa cagtatgata 1261 ctgaagatga caaggtaatg catcattcta tacgtgtcat tctgaacgag gcgcgctttc 1321 cttttttctt tttgcttttt cttttttttt ctcttgaact cgacggatca tatgcggtgt 1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa ggggggatgt gctgcaaggc 1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 2041 aattgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta 2101 gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc 2161 tcgagggggg gcccggtacc cagcttttgt tccctttagt gagggttaat tccgagcttg 2221 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 2281 aacataggag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc 2341 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 2401 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 2461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 2521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 2581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 2641 aggctcggcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 2701 ccgacaggac tataaagata ccaggcgttc ccccctggaa gctccctcgt gcgctctcct 2761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 2821 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 2881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 2941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 3001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 3061 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 3121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 3181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 3241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 3301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 3361 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 3421 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactgcccgt cgtgtagata 3481 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 3541 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 3601 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 3661 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 3721 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 3781 gttacatgat cccccatgtt gtgaaaaaaa gcggttagct ccttcggtcc tccgatcgtt 3841 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 3901 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 3961 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 4021 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 4081 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 4141 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 4201 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 4261 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 4321 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 4381 cctgacgtct tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 4441 gtc //
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