质粒名称: | EF.hICN1.Ubc.GFP |
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出品公司: | 爱迪基因 |
目录编号: | 17626 |
存储实验室: | Linzhao Cheng |
载体骨架基本信息 |
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载体名称: | cFUGW |
载体抗性: | 氨苄霉素 |
空载体大小: | 11500 bp |
载体类型: | 哺乳细胞表达,慢病毒 |
筛选标记: | GFP |
高拷贝/低拷贝: | 未知 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | ICN1 |
物种来源: | H. sapiens (人类) |
插入大小: | 2400 bp |
克隆方法: | 限制性内切酶 |
5' 测序引物 | EF-1a Forward |
文献引用方法 |
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材料和方法部分: | EF.hICN1.Ubc.GFP was a gift from Linzhao Cheng (Addgene plasmid # 17626) |
参考文献部分: | HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI. Stem Cells. 2006 Apr . 24(4):876-88. 10.1634/stemcells.2005-0598 PubMed 16513761 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS EF.hICN1.Ubc.GFP 13830 bp DNA SYN DEFINITION EF.hICN1.Ubc.GFP ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..13830 /organism="EF.hICN1.Ubc.GFP" /mol_type="other DNA" promoter 239..815 /label="CMV_immearly_promoter" misc_feature 318..605 /label="CAG_enhancer" misc_feature 772..792 /label="CMV_fwd_primer" misc_feature 835..1015 /label="HIV-1_5_LTR" misc_feature 835..1015 /label="truncHIV-1_3_LTR" misc_feature 1126..1170 /label="HIV-1_psi_pack" CDS 1558..2445 /label="ORF frame 1" misc_feature 1680..1913 /label="RRE" misc_feature 2444..2459 /label="cPPT" misc_feature 2623..2639 /label="pBluescriptKS_primer" promoter 2726..3909 /label="EF1a_promoter" CDS complement(3029..3649) /label="ORF frame 3" misc_feature 3857..3877 /label="EF1a_fwd_primer" promoter 3926..3944 /label="T7_promoter" CDS 3985..6375 /label="ORF frame 1" promoter 6507..7735 /label="hUbC_promoter" CDS 7762..8481 /label="ORF frame 1" gene 7765..8478 /label="EGFP" /gene="EGFP" misc_feature complement(7807..7828) /label="EGFP_N_primer" misc_feature 7945..7974 /label="Y66 (EGFP)" misc_feature 8415..8436 /label="EGFP_C_primer" misc_feature 8524..9111 /label="WPRE" CDS 8612..9661 /label="ORF frame 2" CDS 8625..9137 /label="ORF frame 3" misc_feature complement(9114..9130) /label="pBluescriptKS_primer" misc_feature 9298..9313 /label="cPPT" misc_feature 9298..9319 /label="U3PPT" misc_feature 9635..9815 /label="truncHIV-1_3_LTR" misc_feature 9635..9815 /label="HIV-1_5_LTR" misc_feature complement(9841..9858) /label="BGH_rev_primer" terminator 9844..10071 /label="bGH_PA_terminator" rep_origin 10134..10440 /label="f1_origin" misc_feature complement(10554..10574) /label="pBABE_3_primer" misc_feature complement(10560..10775) /label="SV40_enhancer" promoter 10572..10840 /label="SV40_promoter" rep_origin 10739..10816 /label="SV40_origin" misc_feature 10801..10820 /label="SV40pro_F_primer" promoter 10934..11001 /label="EM7_promoter" misc_feature 11002..11376 /label="sh_ble" gene 11002..11373 /label="bleo" /gene="bleo" terminator 11509..11628 /label="SV40_PA_terminator" misc_feature 11597..11616 /label="EBV_rev_primer" promoter complement(11672..11690) /label="M13_reverse_primer" misc_feature complement(11689..11711) /label="M13_pUC_rev_primer" promoter complement(11725..11754) /label="lac_promoter" gene complement(12837..13697) /label="Ampicillin" /gene="Ampicillin" CDS complement(12837..13697) /label="ORF frame 2" promoter complement(13739..13767) /label="AmpR_promoter" ORIGIN 1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC 241 ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 301 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG 361 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT 421 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG 481 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC 541 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 601 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT 661 TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC 721 ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG 781 GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGCGC GTTTTGCCTG TACTGGGTCT 841 CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT 901 AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC 961 TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC 1021 GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC 1081 GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA 1141 TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG 1201 GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA 1261 AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC 1321 TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA 1381 CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC 1441 AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA 1501 AAAGTAAGAC CACCGCACAG CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG 1561 AGGGACAATT GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA 1621 GTAGCACCCA CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA 1681 GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG 1741 ACGCTGACGG TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG 1801 CTGAGGGCTA TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG 1861 CTCCAGGCAA GAATCCTGGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT 1921 TGGGGTTGCT CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT 1981 AATAAATCTC TGGAACAGAT TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT 2041 AACAATTACA CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG 2101 AATGAACAAG AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA 2161 ACAAATTGGC TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA 2221 AGAATAGTTT TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA 2281 TCGTTTCAGA CCCACCTCCC AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA 2341 GAAGGTGGAG AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC GGCACTGCGT 2401 GCGCCAATTC TGCAGACAAA TGGCAGTATT CATCCACAAT TTTAAAAGAA AAGGGGGGAT 2461 TGGGGGGTAC AGTGCAGGGG AAAGAATAGT AGACATAATA GCAACAGACA TACAAACTAA 2521 AGAATTACAA AAACAAATTA CAAAAATTCA AAATTTTCGG GTTTATTACA GGGACAGCAG 2581 AGATCCAGTT TGGTTAATTA AgtttaaacG GGCCCTCTAG ActcgagGTC GACGGtATCG 2641 ATAAGCTTTG CAAAGATGGA TAAAGTTTTA AACAGAGAGG AATCTTTGCA GCTAATGGAC 2701 CTTCTAGGTC TTGAAAGGAG TGCCTCGTGA GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC 2761 ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC CGGTGCCTAG 2821 AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC 2881 GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC 2941 GGGTTTGCCG CCAGAACACA GGTAAGTGCC GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT 3001 ACGGGTTATG GCCCTTGCGT GCCTTGAATT ACTTCCACCT GGCTGCAGTA CGTGATTCTT 3061 GATCCCGAGC TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC 3121 CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT GGGGCCGCCG CGTGCGAATC 3181 TGGTGGCACC TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT TTAAAATTTT 3241 TGATGACCTG CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC GGGCCAAGAT 3301 CTGCACACTG GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG 3361 CGCACATGTT CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC CGAGAATCGG ACGGGGGTAG 3421 TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC 3481 TGGGCGGCAA GGCTGGCCCG GTCGGCACCA GTTGCGTGAG CGGAAAGATG GCCGCTTCCC 3541 GGCCCTGCTG CAGGGAGCTC AAAATGGAGG ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG 3601 TCACCCACAC AAAGGAAAAG GGCCTTTCCG TCCTCAGCCG TCGCTTCATG TGACTCCACG 3661 GAGTACCGGG CGCCGTCCAG GCACCTCGAT TAGTTCTCGA GCTTTTGGAG TACGTCGTCT 3721 TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT 3781 GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT 3841 GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG 3901 GTGTCGTGAG GAATTAGCTT GGTACTAATA CGACTCACTA TAGGGAGACC CAAGCTGGCT 3961 AGTTAAGCTT GATgatccgg aggCatgcgg cggcagcatg gccagctctg gttccctgag 4021 ggcttcaaag tgtctgaggc cagcaagaag aagcggcggg agcccctcgg cgaggactcc 4081 gtgggcctca agcccctgaa gaacgcttca gacggtgccc tcatggacga caaccagaat 4141 gagtgggggg acgaggacct ggagaccaag aagttccggt tcgaggagcc cgtggttctg 4201 cctgacctgg acgaccagac agaccaccgg cagtggactc agcagcacct ggatgccgct 4261 gacctgcgca tgtctgccat ggcccccaca ccgccccagg gtgaggttga cgccgactgc 4321 atggacgtca atgtccgcgg gcctgatggc ttcaccccgc tcatgatcgc ctcctgcagc 4381 gggggcggcc tggagacggg caacagcgag gaagaggagg acgcgccggc cgtcatctcc 4441 gacttcatct accagggcgc cagcctgcac aaccagacag accgcacggg cgagaccgcc 4501 ttgcacctgg ccgcccgcta ctcacgctct gatgccgcca agcgcctgct ggaggccagc 4561 gcagatgcca acatccagga caacatgggc cgcaccccgc tgcatgcggc tgtgtctgcc 4621 gacgcacaag gtgtcttcca gatcctgatc cggaaccgag ccacagacct ggatgcccgc 4681 atgcatgatg gcacgacgcc actgatcctg gctgcccgcc tggccgtgga gggcatgctg 4741 gaggacctca tcaactcaca cgccgacgtc aacgccgtag atgacctggg caagtccgcc 4801 ctgcactggg ccgccgccgt gaacaatgtg gatgccgcag ttgtgctcct gaagaacggg 4861 gctaacaaag atatgcagaa caacagggag gagacacccc tgtttctggc cgcccgggag 4921 ggcagctacg agaccgccaa ggtgctgctg gaccactttg ccaaccggga catcacggat 4981 catatggacc gcctgccgcg cgacatcgca caggagcgca tgcatcacga catcgtgagg 5041 ctgctggacg agtacaacct ggtgcgcagc ccgcagctgc acggagcccc gctggggggc 5101 acgcccaccc tgtcgccccc gctctgctcg cccaacggct acctgggcag cctcaagccc 5161 ggcgtgcagg gcaagaaggt ccgcaagccc agcagcaaag gcctggcctg tggaagcaag 5221 gaggccaagg acctcaaggc acggaggaag aagtcccagg atggcaaggg ctgcctgctg 5281 gacagctccg gcatgctctc gcccgtggac tccctggagt caccccatgg ctacctgtca 5341 gacgtggcct cgccgccact gctgccctcc ccgttccagc agtctccgtc cgtgcccctc 5401 aaccacctgc ctgggatgcc cgacacccac ctgggcatcg ggcacctgaa cgtggcggcc 5461 aagcccgaga tggcggcgct gggtgggggc ggccggctgg cctttgagac tggcccacct 5521 cgtctctccc acctgcctgt ggcctctggc accagcaccg tcctgggctc cagcagcgga 5581 ggggccctga atttcactgt gggcgggtcc accagtttga atggtcaatg cgagtggctg 5641 tcccggctgc agagcggcat ggtgccgaac caatacaacc ctctgcgggg gagtgtggca 5701 ccaggccccc tgagcacaca ggccccctcc ctgcagcatg gcatggtagg cccgctgcac 5761 agtagccttg ctgccagcgc cctgtcccag atgatgagct accagggcct gcccagcacc 5821 cggctggcca cccagcctca cctggtgcag acccagcagg tgcagccaca aaacttacag 5881 atgcagcagc agaacctgca gccagcaaac atccagcagc agcaaagcct gcagccgcca 5941 ccaccaccac cacagccgca ccttggcgtg agctcagcag ccagcggcca cctgggccgg 6001 agcttcctga gtggagagcc gagccaggca gacgtgcagc cactgggccc cagcagcctg 6061 gcggtgcaca ctattctgcc ccaggagagc cccgccctgc ccacgtcgct gccatcctcg 6121 ctggtcccac ccgtgaccgc agcccagttc ctgacgcccc cctcgcagca cagctactcc 6181 tcgcctgtgg acaacacccc cagccaccag ctacaggtgc ctgagcaccc cttcctgacc 6241 ccttcgccgg agtcgcccga ccaatggtcg tcctcgtcgc cgcactctaa tgtgtctgac 6301 tggtctgagg gcgtgtcgtc gcccccgacc tccatgcagt cccagatcgc gcgcatcccg 6361 gaggcgttca agTAAacggc gcgtcagatc cactagtaac ggccgccagt gtgctggaat 6421 tATCGAATTC Ccaccacact ggactagtGG ATCCgggggt accgcgtcga gTTAATTAAg 6481 ggcacagccg aggtctagag gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc 6541 ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca ggagcgttcc tgatccttcc 6601 gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat 6661 cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca 6721 gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg 6781 tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg 6841 tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg 6901 agttgcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg 6961 aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa 7021 ctgggggttg gggggagcgc acaaaatggc ggctgttccc gagtcttgaa tggaagacgc 7081 ttgtaaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac 7141 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg 7201 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg ggtttgtcgt 7261 ctggttgcgg gggcggcagt tatgcggtgc cgttgggcag tgcacccgta cctttgggag 7321 cgcgcgcctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc 7381 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta 7441 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg 7501 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt 7561 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa 7621 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaagc ttctgcaggt 7681 cgactctaga aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacaGGAT 7741 CCCCGGGTAC CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG 7801 CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG 7861 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG 7921 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC 7981 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC 8041 GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG 8101 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG 8161 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC 8221 ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG 8281 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC 8341 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC 8401 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC 8461 ATGGACGAGC TGTACAAGTA AAGCGGCCGC GACTCTAGAA TTCGATATCA AGCTTATCGA 8521 TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC 8581 TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG 8641 TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT 8701 GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC 8761 TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC 8821 TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT 8881 GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT 8941 CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT 9001 CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT 9061 TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG 9121 TCGACCTCGA GACCTAGAAA AACATGGAGC AATCACAAGT AGCAATACAG CAGCTACCAA 9181 TGCTGATTGT GCCTGGCTAG AAGCACAAGA GGAGGAGGAG GTGGGTTTTC CAGTCACACC 9241 TCAGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA 9301 AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATA TCCTTGATCT 9361 GTGGATCTAC CACACACAAG GCTACTTCCC TGATTGGCAG AACTACACAC CAGGGCCAGG 9421 GATCAGATAT CCACTGACCT TTGGATGGTG CTACAAGCTA GTACCAGTTG AGCAAGAGAA 9481 GGTAGAAGAA GCCAATGAAG GAGAGAACAC CCGCTTGTTA CACCCTGTGA GCCTGCATGG 9541 GATGGATGAC CCGGAGAGAG AAGTATTAGA GTGGAGGTTT GACAGCCGCC TAGCATTTCA 9601 TCACATGGCC CGAGAGCTGC ATCCGGACTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG 9661 AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC 9721 TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT 9781 CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGGGCC CGTTTAAACC CGCTGATCAG 9841 CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT 9901 TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC 9961 ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG 10021 AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG 10081 CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA 10141 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 10201 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 10261 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 10321 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 10381 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 10441 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 10501 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT 10561 GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 10621 GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG 10681 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT 10741 CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT 10801 TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG 10861 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG 10921 ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC 10981 GACAAGGTGA GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG 11041 CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC 11101 GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC 11161 CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG 11221 CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC 11281 ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC 11341 AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC 11401 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG 11461 ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA 11521 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT 11581 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA 11641 CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT 11701 TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG 11761 GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG 11821 TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT 11881 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 11941 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 12001 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 12061 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 12121 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 12181 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 12241 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 12301 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 12361 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 12421 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 12481 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT 12541 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 12601 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 12661 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 12721 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 12781 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC 12841 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT 12901 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT 12961 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 13021 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT 13081 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT 13141 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC 13201 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT 13261 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG 13321 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG 13381 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT 13441 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC 13501 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT 13561 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT 13621 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG 13681 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 13741 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 13801 CGCACATTTC CCCGAAAAGT GCCACCTGAC //