| 质粒名称: | pdCas9-bacteria |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 44249 |
| 存储实验室: | Stanley Qi |
|
载体骨架基本信息 |
|
| 载体名称: | p15A |
| 载体抗性: | 氯霉素 |
| 完整质粒大小: | 6705 bp |
| 载体类型: | 细菌表达，CRISPR |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dCas9 (细菌) |
| 物种来源: | S.化脓性链球菌 |
| 插入大小: | 4107 bp |
| 基因突变: | D10A H840A (催化活性) |
| 启动子 | pLtetO-1 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BglII(未破坏) |
| 3' 克隆位点: | XhoI (未破坏) |
| 5' 测序引物 | ttcaaaaGATCTAAAGAGGAGAAAGGATCTATGGATAAGAAATACTCAATAG |
| 3' 测序引物 | tgcctggagatccttactcgaGTTAGTCACCTCCTAGCTGACTCAAATCAAT |
|
文献引用方法 |
|
| 材料和方法部分: | pdCas9-bacteria was a gift from Stanley Qi (Addgene plasmid # 44249) |
| 参考文献部分: |
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. 10.1016/j.cell.2013.02.022 PubMed 23452860 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pdCas9-bacteria 6705 bp DNA SYN
DEFINITION pdCas9-bacteria
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6705
/organism="pdCas9-bacteria"
/mol_type="other DNA"
CDS complement(7..633)
/label="ORF frame 1"
misc_feature complement(21..627)
/label="TetR"
CDS 740..4846
/label="ORF frame 2"
terminator 4873..4916
/label="rrnB_T1_terminator"
gene complement(5920..6579)
/label="CAT/CamR"
/gene="CAT/CamR"
CDS complement(5920..6579)
/label="ORF frame 1"
ORIGIN
1 gacgtcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
661 tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagaat tcaaaaGATC
721 TAAAGAGGAG AAAGGATCTA TGGATAAGAA ATACTCAATA GGCTTAGCTA TCGGCACAAA
781 TAGCGTCGGA TGGGCGGTGA TCACTGATGA ATATAAGGTT CCGTCTAAAA AGTTCAAGGT
841 TCTGGGAAAT ACAGACCGCC ACAGTATCAA AAAAAATCTT ATAGGGGCTC TTTTATTTGA
901 CAGTGGAGAG ACAGCGGAAG CGACTCGTCT CAAACGGACA GCTCGTAGAA GGTATACACG
961 TCGGAAGAAT CGTATTTGTT ATCTACAGGA GATTTTTTCA AATGAGATGG CGAAAGTAGA
1021 TGATAGTTTC TTTCATCGAC TTGAAGAGTC TTTTTTGGTG GAAGAAGACA AGAAGCATGA
1081 ACGTCATCCT ATTTTTGGAA ATATAGTAGA TGAAGTTGCT TATCATGAGA AATATCCAAC
1141 TATCTATCAT CTGCGAAAAA AATTGGTAGA TTCTACTGAT AAAGCGGATT TGCGCTTAAT
1201 CTATTTGGCC TTAGCGCATA TGATTAAGTT TCGTGGTCAT TTTTTGATTG AGGGAGATTT
1261 AAATCCTGAT AATAGTGATG TGGACAAACT ATTTATCCAG TTGGTACAAA CCTACAATCA
1321 ATTATTTGAA GAAAACCCTA TTAACGCAAG TGGAGTAGAT GCTAAAGCGA TTCTTTCTGC
1381 ACGATTGAGT AAATCAAGAC GATTAGAAAA TCTCATTGCT CAGCTCCCCG GTGAGAAGAA
1441 AAATGGCTTA TTTGGGAATC TCATTGCTTT GTCATTGGGT TTGACCCCTA ATTTTAAATC
1501 AAATTTTGAT TTGGCAGAAG ATGCTAAATT ACAGCTTTCA AAAGATACTT ACGATGATGA
1561 TTTAGATAAT TTATTGGCGC AAATTGGAGA TCAATATGCT GATTTGTTTT TGGCAGCTAA
1621 GAATTTATCA GATGCTATTT TACTTTCAGA TATCCTAAGA GTAAATACTG AAATAACTAA
1681 GGCTCCCCTA TCAGCTTCAA TGATTAAACG CTACGATGAA CATCATCAAG ACTTGACTCT
1741 TTTAAAAGCT TTAGTTCGAC AACAACTTCC AGAAAAGTAT AAAGAAATCT TTTTTGATCA
1801 ATCAAAAAAC GGATATGCAG GTTATATTGA TGGGGGAGCT AGCCAAGAAG AATTTTATAA
1861 ATTTATCAAA CCAATTTTAG AAAAAATGGA TGGTACTGAG GAATTATTGG TGAAACTAAA
1921 TCGTGAAGAT TTGCTGCGCA AGCAACGGAC CTTTGACAAC GGCTCTATTC CCCATCAAAT
1981 TCACTTGGGT GAGCTGCATG CTATTTTGAG AAGACAAGAA GACTTTTATC CATTTTTAAA
2041 AGACAATCGT GAGAAGATTG AAAAAATCTT GACTTTTCGA ATTCCTTATT ATGTTGGTCC
2101 ATTGGCGCGT GGCAATAGTC GTTTTGCATG GATGACTCGG AAGTCTGAAG AAACAATTAC
2161 CCCATGGAAT TTTGAAGAAG TTGTCGATAA AGGTGCTTCA GCTCAATCAT TTATTGAACG
2221 CATGACAAAC TTTGATAAAA ATCTTCCAAA TGAAAAAGTA CTACCAAAAC ATAGTTTGCT
2281 TTATGAGTAT TTTACGGTTT ATAACGAATT GACAAAGGTC AAATATGTTA CTGAAGGAAT
2341 GCGAAAACCA GCATTTCTTT CAGGTGAACA GAAGAAAGCC ATTGTTGATT TACTCTTCAA
2401 AACAAATCGA AAAGTAACCG TTAAGCAATT AAAAGAAGAT TATTTCAAAA AAATAGAATG
2461 TTTTGATAGT GTTGAAATTT CAGGAGTTGA AGATAGATTT AATGCTTCAT TAGGTACCTA
2521 CCATGATTTG CTAAAAATTA TTAAAGATAA AGATTTTTTG GATAATGAAG AAAATGAAGA
2581 TATCTTAGAG GATATTGTTT TAACATTGAC CTTATTTGAA GATAGGGAGA TGATTGAGGA
2641 AAGACTTAAA ACATATGCTC ACCTCTTTGA TGATAAGGTG ATGAAACAGC TTAAACGTCG
2701 CCGTTATACT GGTTGGGGAC GTTTGTCTCG AAAATTGATT AATGGTATTA GGGATAAGCA
2761 ATCTGGCAAA ACAATATTAG ATTTTTTGAA ATCAGATGGT TTTGCCAATC GCAATTTTAT
2821 GCAGCTGATC CATGATGATA GTTTGACATT TAAAGAAGAC ATTCAAAAAG CACAAGTGTC
2881 TGGACAAGGC GATAGTTTAC ATGAACATAT TGCAAATTTA GCTGGTAGCC CTGCTATTAA
2941 AAAAGGTATT TTACAGACTG TAAAAGTTGT TGATGAATTG GTCAAAGTAA TGGGGCGGCA
3001 TAAGCCAGAA AATATCGTTA TTGAAATGGC ACGTGAAAAT CAGACAACTC AAAAGGGCCA
3061 GAAAAATTCG CGAGAGCGTA TGAAACGAAT CGAAGAAGGT ATCAAAGAAT TAGGAAGTCA
3121 GATTCTTAAA GAGCATCCTG TTGAAAATAC TCAATTGCAA AATGAAAAGC TCTATCTCTA
3181 TTATCTCCAA AATGGAAGAG ACATGTATGT GGACCAAGAA TTAGATATTA ATCGTTTAAG
3241 TGATTATGAT GTCGATGCCA TTGTTCCACA AAGTTTCCTT AAAGACGATT CAATAGACAA
3301 TAAGGTCTTA ACGCGTTCTG ATAAAAATCG TGGTAAATCG GATAACGTTC CAAGTGAAGA
3361 AGTAGTCAAA AAGATGAAAA ACTATTGGAG ACAACTTCTA AACGCCAAGT TAATCACTCA
3421 ACGTAAGTTT GATAATTTAA CGAAAGCTGA ACGTGGAGGT TTGAGTGAAC TTGATAAAGC
3481 TGGTTTTATC AAACGCCAAT TGGTTGAAAC TCGCCAAATC ACTAAGCATG TGGCACAAAT
3541 TTTGGATAGT CGCATGAATA CTAAATACGA TGAAAATGAT AAACTTATTC GAGAGGTTAA
3601 AGTGATTACC TTAAAATCTA AATTAGTTTC TGACTTCCGA AAAGATTTCC AATTCTATAA
3661 AGTACGTGAG ATTAACAATT ACCATCATGC CCATGATGCG TATCTAAATG CCGTCGTTGG
3721 AACTGCTTTG ATTAAGAAAT ATCCAAAACT TGAATCGGAG TTTGTCTATG GTGATTATAA
3781 AGTTTATGAT GTTCGTAAAA TGATTGCTAA GTCTGAGCAA GAAATAGGCA AAGCAACCGC
3841 AAAATATTTC TTTTACTCTA ATATCATGAA CTTCTTCAAA ACAGAAATTA CACTTGCAAA
3901 TGGAGAGATT CGCAAACGCC CTCTAATCGA AACTAATGGG GAAACTGGAG AAATTGTCTG
3961 GGATAAAGGG CGAGATTTTG CCACAGTGCG CAAAGTATTG TCCATGCCCC AAGTCAATAT
4021 TGTCAAGAAA ACAGAAGTAC AGACAGGCGG ATTCTCCAAG GAGTCAATTT TACCAAAAAG
4081 AAATTCGGAC AAGCTTATTG CTCGTAAAAA AGACTGGGAT CCAAAAAAAT ATGGTGGTTT
4141 TGATAGTCCA ACGGTAGCTT ATTCAGTCCT AGTGGTTGCT AAGGTGGAAA AAGGGAAATC
4201 GAAGAAGTTA AAATCCGTTA AAGAGTTACT AGGGATCACA ATTATGGAAA GAAGTTCCTT
4261 TGAAAAAAAT CCGATTGACT TTTTAGAAGC TAAAGGATAT AAGGAAGTTA AAAAAGACTT
4321 AATCATTAAA CTACCTAAAT ATAGTCTTTT TGAGTTAGAA AACGGTCGTA AACGGATGCT
4381 GGCTAGTGCC GGAGAATTAC AAAAAGGAAA TGAGCTGGCT CTGCCAAGCA AATATGTGAA
4441 TTTTTTATAT TTAGCTAGTC ATTATGAAAA GTTGAAGGGT AGTCCAGAAG ATAACGAACA
4501 AAAACAATTG TTTGTGGAGC AGCATAAGCA TTATTTAGAT GAGATTATTG AGCAAATCAG
4561 TGAATTTTCT AAGCGTGTTA TTTTAGCAGA TGCCAATTTA GATAAAGTTC TTAGTGCATA
4621 TAACAAACAT AGAGACAAAC CAATACGTGA ACAAGCAGAA AATATTATTC ATTTATTTAC
4681 GTTGACGAAT CTTGGAGCTC CCGCTGCTTT TAAATATTTT GATACAACAA TTGATCGTAA
4741 ACGATATACG TCTACAAAAG AAGTTTTAGA TGCCACTCTT ATCCATCAAT CCATCACTGG
4801 TCTTTATGAA ACACGCATTG ATTTGAGTCA GCTAGGAGGT GACTAACtcg agtaaggatc
4861 tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
4921 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
4981 tgcgtttata cctagggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg
5041 ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca
5101 ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg
5161 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
5221 actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc
5281 ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga
5341 cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
5401 agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg
5461 caaaagcacc actggcagca gccactggta attgatttag aggagttagt cttgaagtca
5521 tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag
5581 ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg
5641 gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat
5701 cttattaatc agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca
5761 cctgaagtca gccccatacg atataagttg ttactagtgc ttggattctc accaataaaa
5821 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
5881 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
5941 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
6001 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
6061 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
6121 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
6181 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
6241 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
6301 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
6361 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
6421 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
6481 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
6541 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
6601 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
6661 ggaacctctt acgtgccgat caacgtctca ttttcgccag atatc
//
