pCMVLacI

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pCMV-LacI载体基本信息

出品公司: Stratagene
载体名称: pCMVLacI
质粒类型: LacSwith II哺乳动物诱导表达系统
高拷贝/低拷贝: --
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8.0kb
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄
筛选标记: Hygromycin
备注: --
产品目录号: --
稳定性: --
组成型: --
病毒/非病毒: --

pCMV-LacI载体质粒图谱和多克隆位点信息

pCMVLacI载体图谱



pCMVLacI载体特征

pCMV-LacI载体简介

pCMV-LacI载体序列

LOCUS       pCMVLacI                7995 bp    DNA     circular SYN 23-JUL-2016
DEFINITION  Cloning vector pCMVLacI from Lacswitch II System, complete
            sequence.
ACCESSION   U64448
VERSION     U64448.1
KEYWORDS    .
SOURCE      Cloning vector pCMVLacI
  ORGANISM  Cloning vector pCMVLacI
            other sequences; artificial sequences; vectors.
COMMENT     VNTAUTHORNAME|www.biofeng.com|
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Wyborski,D.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-1996) Genetic Systems, Stratagene, 11011 North
            Torrey Pines Road, La Jolla, CA 92037, USA
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Cloning vector pCMVLacI"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:50944"
                     /plasmid="pCMVLacI"
     gene            2685..3803
                     /gene="lacI"
     CDS             2685..3803
                     /gene="lacI"
                     /codon_start=1
                     /transl_table=11
                     /product="lac repressor"
                     /protein_id="AAB17268.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQSSLRPPKKKRKV"
     CDS             4789..5826
                     /codon_start=1
                     /transl_table=11
                     /product="hygromycin B phosphotransferase"
                     /protein_id="AAB17269.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPDREMGEAN"
ORIGIN      
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta cgcgtataga tctagcttcg
      481 tgtcaaggac ggtgactgca gtgaataata aaatgtgtgt ttgtccgaaa tacgcgtttt
      541 gagatttctg tcgccgacta aattcatgtc gcgcgatagt ggtgtttatc gccgatagag
      601 atggcgatat tggaaatcga tatttgaaaa tatggcatat tgaaaatgtc gccgatgtga
      661 gtttctgtgt aactgatatc gccatttttc caaaagtgat ttttgggcat acgcgatatc
      721 tggcgatacg gcttatatcg tttacggggg atggcgatag acgactttgg cgacttgggc
      781 gattctgtgt gtcgcaaata tcgcagtttc gatataggtg acagacgata tgaggctata
      841 tcgccgatag aggcgacatg aagctggcac atggccaatg catatcgatc tatacattga
      901 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
      961 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
     1021 caatatgacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     1081 ggtcattagt tcatagccca tatatggact tccgcgttac ataacttacg gtaaatggcc
     1141 cgcctcgtga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1201 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1261 ccacttggca gtacatcaag tgtatcatat gccaagtccg gccccctatt gacgtcaatg
     1321 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt
     1381 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     1441 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     1501 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc
     1561 ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     1621 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     1681 gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc
     1741 cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc cctttggctc
     1801 ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgctcct tatgctatag
     1861 gtgatggtat agcttagcct ataggtgtgg gttattgacc attattgacc actcccctat
     1921 tggtgacgat actttccatt actaatccat aacatggctc tttgccacaa ctatctctat
     1981 tggcttatgc caatactctg tccttcagag actgacacgg actctgtatt tttacaggat
     2041 ggggtcccat ttattattta caaattcaca tatacaacaa cgccgtcccc cgtgcccgca
     2101 gtttttatta aacatagcgt gggatctcca cgcgaatctc gggtacgtgt tccggacatg
     2161 ggctcttctc cggtagcggc ggagcttcca catccgagcc ctggtcccat gcctccagcg
     2221 gctcatggtc gctcggcagc tccttgctcc taacagtgga ggccagactt aggcacagca
     2281 caatgcccac caccaccagt gtgccgcaca aggccgtggc ggtagggtat gtgtctgaaa
     2341 atgagctcgg agattgggct cgcaccgtga cgcagatgga agacttaagg cagcggcaga
     2401 agaagatgca ggcagctgag ttgttgtatt ctgataagag tcagaggtaa ctcccgttgc
     2461 ggtgctgtta acggtggagg gcagtgtagt ctgagcagta ctcgttgctg ccgcgcgcgc
     2521 caccagacat aatagctgac agactaacag actgttcctt tccatgggtc ttttctgcag
     2581 tcaccgtcct tgacacgaag cttggtaccg agctcggatc tccattcgcc attcaggctg
     2641 cgcaactgtt gggaagggcg atcgtctaga ggtaccctcc caccatgaaa ccagtaacgt
     2701 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
     2761 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
     2821 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
     2881 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     2941 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
     3001 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
     3061 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
     3121 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
     3181 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
     3241 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
     3301 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
     3361 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
     3421 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
     3481 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     3541 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     3601 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     3661 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3721 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagagcagc ctgaggcctc
     3781 ctaagaagaa gaggaaggtt tgagcgcaac gcaattaatg taagttagct cactcattag
     3841 gcaccccagg ctttacactt tatgcttccg acctgcagcc ccttggatct ttgtgaagga
     3901 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     3961 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     4021 attttagatc acagtcccaa ggctcatttc aggcccctca gtcctcacag tctgttcatg
     4081 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     4141 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     4201 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4261 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgggcc
     4321 caagcttggc actgggatct gcgaacgcag caagacgtag cccagcgcgt cggccccgag
     4381 atgcgccgcg tgccgtgctg gagatggcgg accggatgga tatgttctgc caagggttgg
     4441 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     4501 cgttagcgag gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt
     4561 gtccccggaa gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag
     4621 cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc
     4681 cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc
     4741 gcttaacagc gtcaacagcg tgccgcagat cccggggggc aatgagatat gaaaaagcct
     4801 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     4861 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     4921 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     4981 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     5041 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     5101 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     5161 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     5221 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     5281 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     5341 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     5401 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     5461 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     5521 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     5581 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     5641 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     5701 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     5761 ctcgccgata gtggaaaccg acgccccagc actcgtccgg atcgggagat gggggaggct
     5821 aactgaaaca cggaaggaga caataccgga aggaaccgcg ctatgacggc aataaaaaga
     5881 cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     5941 ctggcactct gtcgataccc caccgagacc ccattgggcc aatacgcccg cgtttcttcc
     6001 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     6061 gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc actagctagt
     6121 tctagtatgc atggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6181 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6241 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6301 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6361 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6421 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6481 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6541 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6601 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6661 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     6721 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6781 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6841 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6901 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6961 atcaatctaa agtatatatg agtaacctga ggctgacagt taccaatgct taatcagtga
     7021 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7081 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7141 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7201 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7261 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7321 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7381 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7441 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7501 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7561 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7621 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7681 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7741 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7801 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7861 cctcaggact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7921 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7981 cccgaaaagt gccac
//

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