| 出品公司: | Stratagene |
|---|---|
| 载体名称: | pCMVLacI |
| 质粒类型: | LacSwith II哺乳动物诱导表达系统 |
| 高拷贝/低拷贝: | -- |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8.0kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 筛选标记: | Hygromycin |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
买家导航
pCMV-LacI载体基本信息
pCMV-LacI载体图谱质粒图谱和多克隆位点信息
pCMV-LacI载体简介
pCMV-LacI载体序列
LOCUS pCMVLacI 7995 bp DNA circular SYN 23-JUL-2016
DEFINITION Cloning vector pCMVLacI from Lacswitch II System, complete
sequence.
ACCESSION U64448
VERSION U64448.1
KEYWORDS .
SOURCE Cloning vector pCMVLacI
ORGANISM Cloning vector pCMVLacI
other sequences; artificial sequences; vectors.
COMMENT VNTAUTHORNAME|www.biofeng.com|
REFERENCE 1 (bases 1 to 7995)
AUTHORS Wyborski,D.L.
TITLE Direct Submission
JOURNAL Submitted (18-JUL-1996) Genetic Systems, Stratagene, 11011 North
Torrey Pines Road, La Jolla, CA 92037, USA
FEATURES Location/Qualifiers
source 1..7995
/organism="Cloning vector pCMVLacI"
/mol_type="genomic DNA"
/db_xref="taxon:50944"
/plasmid="pCMVLacI"
gene 2685..3803
/gene="lacI"
CDS 2685..3803
/gene="lacI"
/codon_start=1
/transl_table=11
/product="lac repressor"
/protein_id="AAB17268.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQSSLRPPKKKRKV"
CDS 4789..5826
/codon_start=1
/transl_table=11
/product="hygromycin B phosphotransferase"
/protein_id="AAB17269.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPDREMGEAN"
ORIGIN
1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
421 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta cgcgtataga tctagcttcg
481 tgtcaaggac ggtgactgca gtgaataata aaatgtgtgt ttgtccgaaa tacgcgtttt
541 gagatttctg tcgccgacta aattcatgtc gcgcgatagt ggtgtttatc gccgatagag
601 atggcgatat tggaaatcga tatttgaaaa tatggcatat tgaaaatgtc gccgatgtga
661 gtttctgtgt aactgatatc gccatttttc caaaagtgat ttttgggcat acgcgatatc
721 tggcgatacg gcttatatcg tttacggggg atggcgatag acgactttgg cgacttgggc
781 gattctgtgt gtcgcaaata tcgcagtttc gatataggtg acagacgata tgaggctata
841 tcgccgatag aggcgacatg aagctggcac atggccaatg catatcgatc tatacattga
901 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
961 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
1021 caatatgacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
1081 ggtcattagt tcatagccca tatatggact tccgcgttac ataacttacg gtaaatggcc
1141 cgcctcgtga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
1201 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
1261 ccacttggca gtacatcaag tgtatcatat gccaagtccg gccccctatt gacgtcaatg
1321 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt
1381 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
1441 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
1501 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc
1561 ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
1621 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
1681 gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc
1741 cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc cctttggctc
1801 ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgctcct tatgctatag
1861 gtgatggtat agcttagcct ataggtgtgg gttattgacc attattgacc actcccctat
1921 tggtgacgat actttccatt actaatccat aacatggctc tttgccacaa ctatctctat
1981 tggcttatgc caatactctg tccttcagag actgacacgg actctgtatt tttacaggat
2041 ggggtcccat ttattattta caaattcaca tatacaacaa cgccgtcccc cgtgcccgca
2101 gtttttatta aacatagcgt gggatctcca cgcgaatctc gggtacgtgt tccggacatg
2161 ggctcttctc cggtagcggc ggagcttcca catccgagcc ctggtcccat gcctccagcg
2221 gctcatggtc gctcggcagc tccttgctcc taacagtgga ggccagactt aggcacagca
2281 caatgcccac caccaccagt gtgccgcaca aggccgtggc ggtagggtat gtgtctgaaa
2341 atgagctcgg agattgggct cgcaccgtga cgcagatgga agacttaagg cagcggcaga
2401 agaagatgca ggcagctgag ttgttgtatt ctgataagag tcagaggtaa ctcccgttgc
2461 ggtgctgtta acggtggagg gcagtgtagt ctgagcagta ctcgttgctg ccgcgcgcgc
2521 caccagacat aatagctgac agactaacag actgttcctt tccatgggtc ttttctgcag
2581 tcaccgtcct tgacacgaag cttggtaccg agctcggatc tccattcgcc attcaggctg
2641 cgcaactgtt gggaagggcg atcgtctaga ggtaccctcc caccatgaaa ccagtaacgt
2701 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
2761 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
2821 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
2881 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
2941 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
3001 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
3061 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
3121 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
3181 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
3241 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
3301 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
3361 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
3421 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
3481 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
3541 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
3601 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
3661 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
3721 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagagcagc ctgaggcctc
3781 ctaagaagaa gaggaaggtt tgagcgcaac gcaattaatg taagttagct cactcattag
3841 gcaccccagg ctttacactt tatgcttccg acctgcagcc ccttggatct ttgtgaagga
3901 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
3961 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
4021 attttagatc acagtcccaa ggctcatttc aggcccctca gtcctcacag tctgttcatg
4081 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
4141 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
4201 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
4261 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgggcc
4321 caagcttggc actgggatct gcgaacgcag caagacgtag cccagcgcgt cggccccgag
4381 atgcgccgcg tgccgtgctg gagatggcgg accggatgga tatgttctgc caagggttgg
4441 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
4501 cgttagcgag gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt
4561 gtccccggaa gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag
4621 cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc
4681 cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc
4741 gcttaacagc gtcaacagcg tgccgcagat cccggggggc aatgagatat gaaaaagcct
4801 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
4861 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
4921 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
4981 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
5041 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
5101 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
5161 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
5221 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
5281 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
5341 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
5401 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
5461 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
5521 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
5581 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
5641 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
5701 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
5761 ctcgccgata gtggaaaccg acgccccagc actcgtccgg atcgggagat gggggaggct
5821 aactgaaaca cggaaggaga caataccgga aggaaccgcg ctatgacggc aataaaaaga
5881 cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
5941 ctggcactct gtcgataccc caccgagacc ccattgggcc aatacgcccg cgtttcttcc
6001 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
6061 gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc actagctagt
6121 tctagtatgc atggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
6181 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
6241 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
6301 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
6361 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
6421 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
6481 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
6541 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
6601 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
6661 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
6721 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
6781 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
6841 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
6901 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
6961 atcaatctaa agtatatatg agtaacctga ggctgacagt taccaatgct taatcagtga
7021 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
7081 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
7141 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
7201 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
7261 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
7321 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
7381 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
7441 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
7501 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
7561 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
7621 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
7681 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
7741 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
7801 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
7861 cctcaggact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
7921 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
7981 cccgaaaagt gccac
//
