| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pET102 ； pET102/D-TOPO |
| 质粒类型: | 大肠杆菌表达载体；细菌表达载体；TOPO载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | TOPO |
| 启动子: | T7/lacO |
| 载体大小: | 6315 bp |
| 5' 测序引物及序列: | TrxFus fwd 5’ TTCCTCGACGCTAACCTG 3’ |
| 3' 测序引物及序列: | T7 rev 5’ TAGTTATTGCTCAGCGGTGG 3’ |
| 载体标签: | 硫氧还蛋白Trx tag （N-term）；V5 tag、6xHis tag （C-term） |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | TOP10 |
| 表达菌株: |
BL21-Star(DE3) BL21-Star(DE3) pLysS BL21-TrxB(DE3) Origami2(DE3) |
| 备注: |
pET102含有T7聚合酶结合位点和RBS核糖体结合位点，可以做体外转录和翻译； 硫氧还蛋白Trx 标签可以帮助难溶解的目的蛋白折叠，提高可溶性蛋白产量。 |
| 产品目录号: | K10201 |
| 组成型/诱导型: | 诱导型（IPTG） |
| 病毒/非病毒: | 非病毒 |
买家导航
pET102/D-TOPO载体pET102质粒基本信息
pET102/D-TOPO质粒图谱pET102载体图谱和pET102/D-TOPO载体序列pET102质粒序列多克隆位点信息
pET102/D-TOPO质粒pET102载体简介
Champion™ pET102 Directional TOPO® Expression Kit
The Champion™ pET Expression System yields the highest-level protein production in
E. coli. During expression, your protein of interest can reach levels greater than
50 percent of total cellular protein. Based on T7 expression vectors originally
developed by Studier and colleagues (1-3), high-level expression is achieved
because the T7 RNA polymerase is more processive than native E. coliRNA polymerase
and is dedicated to the transcription of your gene of interest. Protein production
is further enhanced in the system by the expression strain BL21 Star™ E. coli,
which significantly improves the stability of mRNA transcripts and increases protein
expression up to ten-fold.
Simplified, efficient Directional TOPO® cloning allows you to quickly enter a
Champion™ pET Expression vector. These kits feature linearized, topoisomerase
I-activated Champion™ pET expression vectors for five-minute directional cloning.
Directional TOPO® Cloning technology facilitates gene expression because:
• A proofreading enzyme is used for PCR, resulting in fewer errors in cloned genes
• Greater than 90% of the clones are in the correct orientation for gene expression,
reducing the time spent on colony screening
Seven Champion™ pET Directional TOPO® Expression Vectors are available: Each vector
carries a T7lac promoter for high-level expression. Flexible options for simplifying
protein detection, cleaving purification tags, selecting plasmid carrying clones,
and/or improving protein yields are available.
With the Champion™ pET Directional TOPO® vectors you can expect highest-level protein
production. Figure 2 shows expression of the lacZ gene in Champion™ pET Directional
TOPO® vectors. demonstrates efficient cleavage using TEV protease of the
N-terminal tag of a β-galactosidase fusion protein expressed from pET151/D-TOPO®.
pET102/D-TOPO质粒序列pET102载体序列
LOCUS pET102 Directional TOPO 6315 bp DNA SYN
DEFINITION pET102 Directional TOPO
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6315
/organism="pET102 Directional TOPO"
/mol_type="other DNA"
misc_feature 106..125
/label="pBRrevBam_primer"
promoter 209..227
/label="T7_promoter"
misc_feature 227..254
/label="lacO"
CDS complement(256..768)
/label="ORF frame 1"
/translation="MVMVMMTGTRRIETEERVRDRLTFKLELALGEGINSQLVIVITG
SRAGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQSRDTTDAIFRRSARVVIDVQFCNG
QFALIFVSDFIQNRSDHFARTAPVCPEINQDCTICLKYISIKRIISQMNNFIRSHYVY
LLLKVKQNYF*"
misc_feature 272..288
/label="T7_transl_en_RBS"
/translation="MVMVMMTGTRRIETEERVRDRLTFKLELALGEGINSQLVIVITG
SRAGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQSRDTTDAIFRRSARVVIDVQFCNG
QFALIFVSDFIQNRSDHFARTAPVCPEINQDCTICLKYISIKRIISQMNNFIRSHYVY
LLLKVKQNYF*"
misc_feature 298..626
/label="His_patch_thio"
/translation="MVMVMMTGTRRIETEERVRDRLTFKLELALGEGINSQLVIVITG
SRAGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQSRDTTDAIFRRSARVVIDVQFCNG
QFALIFVSDFIQNRSDHFARTAPVCPEINQDCTICLKYISIKRIISQMNNFIRSHYVY
LLLKVKQNYF*"
CDS 298..771
/label="ORF frame 1"
/translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
misc_feature 643..657
/label="EK"
/translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
misc_feature 700..741
/label="V5"
/translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
misc_feature 751..768
/label="6xHIS"
/translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
terminator 783..911
/label="T7_terminator"
/translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
misc_feature complement(825..843)
/label="T7_Terminal_primer"
/translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
promoter 1436..1464
/label="AmpR_promoter"
/translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
gene 1506..2366
/label="Ampicillin"
/gene="Ampicillin"
/translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
CDS 1506..2366
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 2521..3140
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3536..3558
/label="pGEX_3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(3552..3743)
/label="ROP"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS complement(4382..4999)
/label="ORF frame 3"
/translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSIIG
PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
CDS 4577..5272
/label="ORF frame 2"
/translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVAL
TARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAAS
GPRWFAPAGENPV*"
misc_feature complement(5055..6146)
/label="lacI"
/translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVAL
TARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAAS
GPRWFAPAGENPV*"
CDS complement(5055..6014)
/label="ORF frame 2"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
CDS complement(5887..72)
/label="ORF frame 1"
/translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
ANCRGD*"
ORIGIN
1 CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA
61 ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT
121 ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA
181 GAGGATCGAG ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG
241 GATAACAATT CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACATAATG
301 GGATCTGATA AAATTATTCA TCTGACTGAT GATTCTTTTG ATACTGATGT ACTTAAGGCA
361 GATGGTGCAA TCCTGGTTGA TTTCTGGGCA CACTGGTGCG GTCCGTGCAA AATGATCGCT
421 CCGATTCTGG ATGAAATCGC TGACGAATAT CAGGGCAAAC TGACCGTTGC AAAACTGAAC
481 ATCGATCACA ACCCGGGCAC TGCGCCGAAA TATGGCATCC GTGGTATCCC GACTCTGCTG
541 CTGTTCAAAA ACGGTGAAGT GGCGGCAACC AAAGTGGGTG CACTGTCTAA AGGTCAGTTG
601 AAAGAGTTCC TCGACGCTAA CCTGGCCGGC TCTGGATCCG GTGATGACGA TGACAAGCTG
661 GGAATTGATC CCTTCACCAA GGGCGAGCTC AAGCTTGAAG GTAAGCCTAT CCCTAACCCT
721 CTCCTCGGTC TCGATTCTAC GCGTACCGGT CATCATCACC ATCACCATTG AGTTTGATCC
781 GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT
841 AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC
901 TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC
961 CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA
1021 CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG
1081 CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC
1141 CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT
1201 TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT
1261 AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTAATTC TTGAAGACGA AAGGGCCTCG
1321 TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG
1381 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA
1441 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA
1501 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC
1561 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG
1621 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC
1681 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT
1741 TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG
1801 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG
1861 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA
1921 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC
1981 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA
2041 CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC
2101 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC
2161 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG
2221 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA
2281 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG
2341 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA
2401 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC
2461 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
2521 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
2581 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
2641 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT
2701 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
2761 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
2821 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
2881 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
2941 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
3001 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
3061 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
3121 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
3181 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT
3241 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
3301 CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA
3361 ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG
3421 CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG
3481 CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG
3541 AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA
3601 AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC
3661 TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG
3721 GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG
3781 GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT
3841 GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG
3901 AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG
3961 GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC
4021 CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC
4081 GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC
4141 TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG
4201 CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG
4261 GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG
4321 AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA
4381 TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT
4441 ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA
4501 TCGCCGTGAC GATCAGCGGT CCAATGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC
4561 CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG
4621 GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG
4681 TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT
4741 GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA
4801 AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT
4861 CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA
4921 CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT
4981 TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT
5041 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT
5101 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT
5161 CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT
5221 TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT
5281 AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC
5341 GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG
5401 TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA
5461 AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA
5521 GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC
5581 GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA
5641 CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT
5701 AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA
5761 TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG
5821 GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG
5881 CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA
5941 ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA
6001 TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC
6061 TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT
6121 AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC
6181 ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG
6241 CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC
6301 AAGGAATGGT GCATG
//
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pET102/D-TOPO质粒pET102载体应用举例
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