| 质粒名称: | GFP-hPIKfyve |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 121148 |
| 存储实验室: | Geert van den Bogaart |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 载体出品公司: | Clontech |
| 空载体大小: | -- |
| 完整质粒大小: | 11028 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达 |
| 筛选标记: | -- |
| 载体抗性: | 卡那霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 7026 bp |
| 基因突变: | -- |
| GenBank编号 | NP_055855.2 |
| 启动子: | CMV |
| 标签/融合蛋白: | GFP（插入物上的N端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI(未破坏) |
| 3’克隆位点: | BamHI (未破坏) |
| 5’测序引物 | CATGGTCCTGCTGGAGTTCGTG |
| 3’测序引物 | GAAATTTGTGATGCTATTGC |
|
文献引用方法 |
|
| 材料和方法部分: | GFP-hPIKfyve was a gift from Geert van den Bogaart (Addgene plasmid # 121148 ; http://n2t.net/addgene:121148 ; RRID:Addgene_121148) |
| 参考文献部分: | The Phosphoinositide Kinase PIKfyve Promotes Cathepsin-S-Mediated Major Histocompatibility Complex Class II Antigen Presentation. Baranov MV, Bianchi F, Schirmacher A, van Aart MAC, Maassen S, Muntjewerff EM, Dingjan I, Ter Beest M, Verdoes M, Keyser SGL, Bertozzi CR, Diederichsen U, van den Bogaart G. iScience. 2019 Jan 25;11:160-177. doi: 10.1016/j.isci.2018.12.015. Epub 2018 Dec 20. 10.1016/j.isci.2018.12.015 PubMed 30612035 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS GFP-hPIKfyve 11028 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses GFP-tagged human PIKfyve in mammalian cells..
ACCESSION .
VERSION .
KEYWORDS GFP-hPIKfyve
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11028)
AUTHORS Baranov MV, Bianchi F, Schirmacher A, van Aart MAC, Maassen S,
Muntjewerff EM, Dingjan I, Ter Beest M, Verdoes M, Keyser SGL,
Bertozzi CR, Diederichsen U, van den Bogaart G
TITLE The Phosphoinositide Kinase PIKfyve Promotes Cathepsin-S-Mediated
Major Histocompatibility Complex Class II Antigen Presentation.
JOURNAL iScience. 2019 Jan 25;11:160-177. doi: 10.1016/j.isci.2018.12.015.
Epub 2018 Dec 20.
PUBMED 30612035
REFERENCE 2 (bases 1 to 11028)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11028
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 1..589
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 490..509
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
enhancer 764..1067
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1068..1271
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1222..1242
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 1310..1319
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1316..2032
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 1316..2032
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1361..1382)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1622..1641)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1969..1990
/label=EGFP-C
/note="EGFP, forward primer"
CDS 4775..4786
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 8519..8640
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8556..8575)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8610..8629
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(8647..9102)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8784..8805)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 8996..9015
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 9129..9233
/gene="bla"
/label=AmpR promoter
promoter 9235..9592
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(9258..9278)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 9443..9578
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9505..9524
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 9627..10421
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(9681..9700)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 10291..10310
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(10609..10628)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 10653..10700
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
1261 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt
1321 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
1381 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
1441 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
1501 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
1561 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
1621 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
1681 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
1741 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
1801 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
1861 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
1921 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
1981 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact
2041 cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc cggtcaccat
2101 ggccacagat gataagacgt ccccaacact ggactctgct aatgatttgc ctcgatctcc
2161 tactagtcct tctcatctca cacactttaa acctttgact cctgatcaag atgagccccc
2221 ttttaaatca gcttatagtt cttttgtaaa tctctttcgt tttaacaaag agagagcaga
2281 aggaggccag ggagaacagc agcctttgag tggaagttgg accagccctc agctcccttc
2341 gaggacacag tctgttaggt cacccacacc ttataaaaag cagcttaatg aggaactcca
2401 gcggcgctct tcagcattag acacaagaag gaaagcagaa cctacctttg gaggtcatga
2461 ccctcgtaca gctgttcagc ttcgaagcct cagcacagta ttaaaacgcc tcaaggaaat
2521 catggagggg aaaagccagg atagtgacct gaaacaatac tggatgccag atagccaatg
2581 taaagagtgc tatgactgta gtgagaaatt tacaaccttt aggcgcagac accattgccg
2641 actatgtggg cagattttct gcagtcgttg ctgtaatcaa gaaatccctg gaaaatttat
2701 gggctataca ggagacctcc gagcttgcac atattgtaga aaaatagcct taagttatgc
2761 tcattccaca gacagtaatt ctattgggga agacttgaat gctctttcag attctgcttg
2821 ctctgtgtct gtgcttgatc caagtgaacc ccgaacacct gttgggagta ggaaagccag
2881 ccgtaacata tttttagagg atgatttggc ctggcaaagt ttgattcatc cagattcctc
2941 aaatactcct ctttcaacaa gacttgtatc tgtgcaagag gatgctggga aatctcctgc
3001 tcgaaataga tcagccagca ttactaacct gtcactggat agatctggtt ctcctatggt
3061 accttcatat gagacatctg tcagtcccca ggctaaccga acatatgtta ggacagagac
3121 cactgaggat gaacgcaaaa ttcttctgga cagtgtgcag ttaaaagacc tgtggaaaaa
3181 aatctgccat cacagcagtg gaatggagtt tcaggatcac cgctactggt tgagaacgca
3241 tcccaactgc attgtaggaa aggaattagt caactggcta atccgaaatg ggcatattgc
3301 cacaagggca caagctatag caattggaca agcaatggtt gatggacgtt ggctggattg
3361 tgttagtcat cacgaccagc ttttcagaga tgagtatgcg ctgtatagac cactgcagag
3421 tacagaattt tctgagacgc cttctcccga cagtgactca gtgaactccg tggaaggaca
3481 ctctgagcca tcctggttta aagacataaa gtttgatgac agtgacacag aacagatagc
3541 tgaagaaggt gacgataatt tggctaattc tgccagtcct agcaagcgca catcagtcag
3601 cagtttccag tccacagtgg acagtgactc agccgcttct atcagcctga acgtggagct
3661 ggacaacgtg aacttccata tcaagaagcc ctccaagtac ccacatgtgc cccctcaccc
3721 tgctgaccaa aaagagtatt tgatttctga cactggagga caacagctct caataagtga
3781 cgctttcatc aaagaatcct tatttaatcg ccgagtagag gaaaaatcca aagagctgcc
3841 tttcacacct ttgggctggc atcataacaa cctggagctc ctgagggagg agaatgggga
3901 gaaacaagcc atggagaggt tgctttcagc taatcataac cacatgatgg cactactcca
3961 gcagttgctc catagtgact cactgtcatc atcttggagg gacatcatcg tgtcattggt
4021 ctgccaggtt gttcagacag tccgacctga tgtcaagaac caggatgatg acatggatat
4081 ccgtcagttt gtccacatca aaaaaatccc aggtggaaag aagtttgatt ctgtggttgt
4141 caatggcttt gtttgtacca agaacattgc acataaaaag atgagttctt gtattaaaaa
4201 ccccaaaatt cttctgttga agtgttccat tgagtatctc tacagagaag aaactaagtt
4261 tacttgcatt gatcctattg tgcttcagga aagggaattc ttgaagaatt atgtccagcg
4321 aatagttgat gttcgaccca ccttggttct tgttgagaaa acagtgtctc ggattgccca
4381 ggacatgtta ttggaacatg gcattacttt ggtcattaat gtaaagtcac aagttttgga
4441 acgaatcagt cgaatgaccc aaggtgattt agtgatgtca atggaccagc tgcttacgaa
4501 accacacctg ggcacttgtc acaaatttta tatgcagata tttcagttgc ctaatgaaca
4561 aaccaagaca ctgatgtttt ttgaaggttg tccacagcac ctaggctgta caatcaagct
4621 aagaggaggc tctgattatg agctggctcg agttaaggag atcctaatat ttatgatctg
4681 tgttgcttat cattctcaac tagaaatatc ctttctcatg gatgaatttg ctatgcctcc
4741 cacattaatg caaaaccctt cattccattc cctgattgag ggacgagggc atgagggggc
4801 tgtccaagag cagtacggtg gaggttccat cccctgggat cctgacatcc ctcctgagtc
4861 tctgccctgt gatgatagca gtttgctgga attgaggatt gtgtttgaga agggtgagca
4921 ggaaaataaa aatcttccgc aggctgttgc ctctgtgaag catcaagaac atagcacaac
4981 agcttgcccg gcgggtctcc cttgtgcttt ctttgcacct gtaccggaat cattgttgcc
5041 actccctgtg gatgaccaac aagatgcttt aggcagcgag cagccagaga ctttgcagca
5101 aacagttgtg ctgcaggatc ccaaaagcca gataagagcc tttagagacc ctctacagga
5161 tgacactgga ttatatgtta ctgaggaagt cacctcctct gaagataaac gaaagactta
5221 ttctttggcc tttaagcagg aattaaaaga tgtgatcctc tgtatctccc cagtaatcac
5281 attccgagaa ccctttcttt taactgaaaa ggggatgaga tgctctaccc gagattattt
5341 tgcagagcag gtttactggt ctcctctcct caataaagaa ttcaaagaaa tggagaacag
5401 gaggaagaaa cagctgctca gggatctctc tggacttcag ggcatgaatg gaagtattca
5461 ggccaagtct attcaagtct taccctcaca tgagctagtg agcactagaa ttgctgagca
5521 tctgggcgat agccagagct tgggtagaat gctggccgat tatcgagcca gaggaggaag
5581 aattcagccc aaaaattcag acccttttgc tcattcaaag gatgcatcaa gtacttcaag
5641 tggccaatca ggaagcaaaa atgagggtga tgaagagaga gggcttattc tgagtgatgc
5701 tgtgtggtca acaaaggtgg actgtctgaa tcccattaat caccagagac tttgtgtgct
5761 cttcagcagc tcttctgccc agtccagcaa tgctcctagt gcctgtgtca gtccttggat
5821 tgtaacaatg gaattttatg gaaagaatga tcttacatta ggaatatttt tagagagata
5881 ctgtttcagg ccttcttatc agtgtccaag catgttctgt gataccccca tggtacatca
5941 tattcggcgc tttgttcatg gccaaggctg tgtgcagata atcctgaagg agttggattc
6001 tccagtacct ggatatcagc atacaattct tacatattcc tggtgtagaa tctgcaaaca
6061 ggtaacacca gttgttgctc tttccaatga gtcctggtct atgtcatttg caaaatacct
6121 tgaacttagg ttttatgggc accagtatac tcgcagagcc aacgctgagc cctgtggtca
6181 ctccatccat catgattatc accagtattt ctcctataac cagatggtgg cgtctttcag
6241 ttattctccc attcggcttc ttgaagtatg tgttccactc cccaaaatat tcattaagcg
6301 tcaggcccca ttaaaagtgt cccttcttca ggatctgaag gacttctttc aaaaagtttc
6361 acaggtatat gttgccattg atgaaagact tgcatctttg aaaactgata catttagtaa
6421 aacaagagag gaaaaaatgg aagatatttt tgcacagaaa gagatggaag aaggtgagtt
6481 caagaactgg attgagaaga tgcaagcaag gctcatgtct tcctctgtag atacccctca
6541 gcaactgcag tcggtctttg agtcactcat tgccaagaaa caaagtctct gtgaagtgct
6601 gcaagcttgg aataacaggt tgcaggacct tttccaacag gaaaagggta gaaagagacc
6661 ttcagttcct ccaagtcctg gaagactgag acaaggggaa gaaagcaaga taagtgcgat
6721 ggatgcatct ccacggaata tttctccagg acttcagaat ggagaaaaag aggatcgctt
6781 cttaacaact ttgtccagcc agagctccac cagttctact catctccaat tgcctacgcc
6841 acctgaagtc atgtctgaac agtcagtggg agggccccct gagctagata cagccagcag
6901 ttccgaagat gtgtttgatg ggcatttgct gggatccaca gacagccaag tgaaggaaaa
6961 gtcaaccatg aaagccatct ttgcaaattt gcttccagga aatagctata atcctattcc
7021 atttcctttt gatccagata aacactactt aatgtatgaa catgaacgag tgcccattgc
7081 agtctgcgag aaggaaccca gctccatcat tgcttttgct ctcagttgta aagaataccg
7141 aaatgcctta gaggaattgt ctaaagcgac tcagtggaac agtgccgaag aagggcttcc
7201 aacaaatagt acttcagata gcagaccaaa gagtagcagc cctatcagat tacctgaaat
7261 gagtggagga cagacaaatc gtacaacaga aacagaacca caaccaacca aaaaggcttc
7321 tggaatgttg tccttcttca gagggacagc agggaaaagc cccgatctct cttcccagaa
7381 gagagagacc ttacgtggag cagatagtgc ttactaccag gttgggcaga cgggcaagga
7441 ggggaccgag aatcaaggcg ttgagcctca agatgaagta gatggaggag atacacaaaa
7501 gaagcaactc ataaatcctc atgtggaact tcaattttca gatgctaatg ccaagtttta
7561 ctgtcggctc tactatgcgg gagagtttca taagatgcgt gaagtgattc tggacagcag
7621 tgaagaagat ttcattcgtt ccctctccca ctcatcaccc tggcaggccc ggggaggcaa
7681 atcaggagct gccttctatg caactgagga tgatagattt attttgaagc aaatgcctcg
7741 tctggaagtc cagtccttcc tcgactttgc accacattac ttcaattata ttacaaatgc
7801 tgttcaacaa aagaggccca cggcgttggc caaaattctt ggagtttaca gaattggtta
7861 taagaactct cagaacaaca ctgagaagaa gttagatctc cttgtcatgg aaaatctttt
7921 ctacgggaga aagatggcac aggtttttga tttgaagggc tctcttagga atcggaatgt
7981 aaaaactgac actggaaaag agagttgtga tgtggtcctg ctagatgaaa atctcctaaa
8041 gatggttcga gacaaccctc tatatattcg ttctcattcc aaagctgtgc tgagaacctc
8101 gatccatagt gactcccatt tcctttctag ccacctcatt atagattatt ctttgctggt
8161 tgggcgagat gatactagca atgagctagt agttggaatt atagattata ttcgaacatt
8221 tacatgggac aaaaagcttg agatggttgt gaaatcaaca ggaattttag gtggacaagg
8281 taaaatgcca acagtggtgt ctccggagtt gtacaggact aggttttgtg aggcaatgga
8341 caagtatttc ctaatggtac cagaccactg gacaggcttg ggtctgaatt gctgaaatca
8401 agctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
8461 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
8521 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
8581 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
8641 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
8701 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
8761 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
8821 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
8881 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
8941 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
9001 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
9061 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
9121 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
9181 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
9241 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
9301 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
9361 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
9421 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
9481 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
9541 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
9601 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
9661 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
9721 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
9781 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
9841 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
9901 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
9961 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
10021 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
10081 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
10141 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
10201 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
10261 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
10321 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
10381 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
10441 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
10501 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
10561 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
10621 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
10681 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
10741 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
10801 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
10861 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
10921 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
10981 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
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