pCMV-LacZ

价格:1500元

联系方式:I47-825O-882O

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pCMVLacZ载体基本信息

出品公司: Clontech
载体名称: pCMV-LacZ,pCMVLacZ
质粒类型: 哺乳动物表达载体;β-gal报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: --
启动子: CMV
载体大小: 7.2 kb
5' 测序引物及序列: CMV Forward CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记:
克隆菌株: DH5a 或 HB101
宿主细胞(系): --
备注: pCMV-LacZ载体是β半乳糖甘酶表达载体,在哺乳动物细胞中高水平表达β半乳糖甘酶;
可用于建立标准浓度或者标准菌株,定量分析其它报告型载体的表达情况。
产品目录号: 631719
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pCMVLacZ载体质粒图谱和多克隆位点信息

pCMV-LacZ载体图谱



pCMV-LacZ 载体特征

pCMVLacZ载体简介


pCMV-LacZ(pCMVLacZ) is a mammalian reporter vector designed to expression β-galactosidase in mammalian cells from the human cytomegalovirus immediate early gene promoter (1). pCMV-LacZ contains an intron (splice donor/splice acceptor; 2) and polyadenylation signal from SV40, and the full-length E. coli β-galactosidase gene with eukaryotic translation initiation signals (3). pCMV-LacZ expresses high levels of β-galactosidase and can be used as a reference (control) plasmid when transfecting other reporter gene constructs and can be used to optimize transfection protocols by employing standard assays or stains to assay β-galactosidase activity. Alternatively, the β-galactosidase gene can be excised using the Not I sites at each end to allow other genes to be inserted into the pCMV-LacZ vector backbone for expression in mammalian cells or to insert the β-galactosidase fragment into another expression vector.

pCMVLacZ载体序列

LOCUS       pCMV-LacZ               7164 bp ds-DNA     circular SYN 19-OCT-2017
DEFINITION  Plasmid for high-level expression of E. coli beta-galactosidase in 
            mammalian cells. Also known as pCMV-beta, pCMVbeta, or pCMV-beta.
ACCESSION   U02451
VERSION     .
KEYWORDS    pCMV-LacZ 
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   MacGregor GR, Caskey CT.
  TITLE     Construction of plasmids that express E. coli beta-galactosidase in 
            mammalian cells.
  JOURNAL   Nucleic Acids Res. 1989;17:2365.
  PUBMED    2495524
REFERENCE   2  (bases 1 to 7164)
  AUTHORS   Clontech (TaKaRa)
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, September 8, 2020 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Cloning vector pCMVbeta"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:31798"
     enhancer        27..330
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          672..768
                     /note="SV40 intron"
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             873..965
                     /codon_start=1
                     /gene="ADH-71k   "
                     /product="N-terminal fragment of Drosophila melanogaster 
                     alcohol dehydrogenase"
                     /note="Drosophila Adh"
                     /translation="MSFTLTNKNVIFVAGLGGIGLDTSKELLKRD"
     CDS             969..4016
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /note="lacZ"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     polyA_signal    4303..4437
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4950..5538)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5709..6569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6570..6674)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 gaattcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tcgaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg gccgcaattc ccggggatcg
      841 aaagagcctg ctaaagcaaa aaagaagtca ccatgtcgtt tactttgacc aacaagaacg
      901 tgattttcgt tgccggtctg ggaggcattg gtctggacac cagcaaggag ctgctcaagc
      961 gcgatcccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     1021 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     1081 gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac
     1141 cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg
     1201 tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc
     1261 ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca
     1321 catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg
     1381 ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc
     1441 gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg
     1501 tgatggtgct gcgttggagt gacggcagtt atctggaaga tcaggatatg tggcggatga
     1561 gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc
     1621 atgttgccac tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga
     1681 tgtgcggcga gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc
     1741 aggtcgccag cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtggttatg
     1801 ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc
     1861 cgaatctcta tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag
     1921 aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg
     1981 gcaagccgtt gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg
     2041 tcatggatga gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg
     2101 ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg
     2161 gcctgtatgt ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc
     2221 tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc
     2281 gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg
     2341 ctaatcacga cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt
     2401 atgaaggcgg cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg
     2461 tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc
     2521 tacctggaga gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc
     2581 ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct
     2641 tcgtctggga ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt
     2701 cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc
     2761 tggtctttgc cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt
     2821 ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc
     2881 atagcgataa cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg
     2941 gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac
     3001 cgcagccgga gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga
     3061 ccgcatggtc agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc
     3121 tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg
     3181 atttttgcat cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt
     3241 cacagatgtg gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc
     3301 gtgcaccgct ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct
     3361 gggtcgaacg ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca
     3421 cggcagatac acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg
     3481 ggaaaacctt atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga
     3541 ttaccgttga tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact
     3601 gccagctggc gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact
     3661 atcccgaccg ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt
     3721 ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt
     3781 atggcccaca ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc
     3841 aactgatgga aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata
     3901 tcgacggttt ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg
     3961 aattacagct gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat
     4021 aaccgggcag gccatgtctg cccgtatttc gcgtaaggaa atccattatg tactatttaa
     4081 aaaacacaaa cttttggatg ttcggtttat tctttttctt ttactttttt atcatgggag
     4141 cctacttccc gtttttcccg atttggctac atgacatcaa ccatatcagc aaaagtgata
     4201 cgggtattat ttttgccgct atttctctgt tctcgctatt attccaaccg ctgtttggtc
     4261 tgctttctga caaactcggc ctcgactcta ggcggccgcg gggatccaga catgataaga
     4321 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     4381 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     4441 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttcg
     4501 gatcctctag agtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt
     4561 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4621 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4681 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     4741 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4801 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4861 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4921 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4981 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5041 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5101 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5161 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5221 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5281 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5341 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     5401 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5461 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5521 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5581 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5641 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5701 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5761 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5821 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5881 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5941 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6001 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6061 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6121 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6181 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6241 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6301 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6361 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6421 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6481 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6541 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6601 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6661 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     6721 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     6781 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     6841 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     6901 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     6961 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     7021 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     7081 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     7141 cgacgttgta aaacgacggc cagt
//

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