质粒名称: | 6xHis-MBP-BrCas12b-RFND |
---|---|
出品公司: | Addgene |
目录编号: | 195339 |
存储实验室: | Piyush Jain |
载体骨架基本信息 |
|
载体名称: | pET |
载体出品公司: | Scott Gradia Lab (Addgene plasmid # 29656) |
空载体大小: | 6401 bp |
完整质粒大小: | 9670 bp |
载体类型: | Bacterial Expression, CRISPR, Synthetic Biology |
载体抗性: | Kanamycin |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 高拷贝 |
插入基因信息 |
|
插入基因名称: | BrCas12b |
物种来源: | Brevibacillus sp. SYP-B805 |
插入大小: | 3269 bp |
基因突变: | R160E, F208W, N524V, D868V |
GenBank编号 | WP_165214399.1 |
启动子: | T7 |
标签/融合蛋白: |
6xHis, MBP, TEV cleavage site (N terminal on backbone)
6xHis (C terminal on insert)
|
克隆方法: | Gibson Cloning |
5’测序引物 | TAATACGACTCACTATAGGG |
3’测序引物 | GCTAGTTATTGCTCAGCGG |
文献引用方法 |
|
材料和方法部分: |
6xHis-MBP-BrCas12b-RFND was a gift from Piyush Jain (Addgene plasmid # 195339 ; http://n2t.net/addgene:195339 ; RRID:Addgene_195339)
|
参考文献部分: | Engineering highly thermostable Cas12b via de novo structural analyses for one-pot detection of nucleic acids. Nguyen LT, Rananaware SR, Yang LG, Macaluso NC, Ocana-Ortiz JE, Meister KS, Pizzano BLM, Sandoval LSW, Hautamaki RC, Fang ZR, Joseph SM, Shoemaker GM, Carman DR, Chang L, Rakestraw NR, Zachary JF, Guerra S, Perez A, Jain PK. Cell Rep Med. 2023 May 16;4(5):101037. doi: 10.1016/j.xcrm.2023.101037. Epub 2023 May 8. 10.1016/j.xcrm.2023.101037 PubMed 37160120 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS 6xHis-MBP-BrCas12b-RFND 9670 bp ds-DNA circular SYN 27-FEB-2023 DEFINITION Expresses Engineered BrCas12b in Bacteria. ACCESSION . VERSION . KEYWORDS 6xHis-MBP-BrCas12b-RFND SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9670) TITLE Engineering Highly Thermostable Cas12b via De Novo Structural Analyses for One-Pot Detection of Nucleic Acids JOURNAL Unpublished REFERENCE 2 (bases 1 to 9670) AUTHORS . TITLE Direct Submission JOURNAL Exported Feb 27, 2023 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..9670 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 106..125 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind 209..228 /label=T7 /note="T7 promoter, forward primer" promoter 209..227 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 228..252 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 267..289 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 308..325 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 335..1435 /codon_start=1 /gene="malE (mutated)" /product="maltose binding protein from E. coli" /label=MBP /note="This version of the gene does not encode a signal sequence, so MBP will remain in the cytosol." /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF WYAVRTAVINAASGRQTVDEALKDAQT" primer_bind 1409..1432 /label=MBP-F /note="Maltose binding protein, forward primer" CDS 1448..1468 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" CDS 4742..4759 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(4812..4830) /label=T7 Term /note="T7 terminator, reverse primer" terminator 4826..4873 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 4910..5365 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4997..5016) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5207..5228 /label=F1ori-F /note="F1 origin, forward primer" CDS complement(5458..6273) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 6181..6200 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 6265..6284 /label=pENTR-R /note="pENTR vectors, reverse primer" rep_origin 6395..6983 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6884..6903 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7137..7154 /label=L4440 /note="L4440 vector, forward primer" misc_feature 7169..7308 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(7234..7253) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7394..7416 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(7410..7601) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" CDS complement(8410..9492) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" primer_bind 9454..9473 /label=LacI-R /note="LacI, reverse primer" promoter complement(9493..9570) /gene="lacI" /label=lacI promoter ORIGIN 1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa 61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat 121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta 181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg 241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat ataccatggg 301 ttcttctcac catcaccatc accatggttc ttctatgaaa atcgaagaag gtaaactggt 361 aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta agaaattcga 421 gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag agaaattccc 481 acaggttgcg gcaactggcg atggccctga cattatcttc tgggcacacg accgctttgg 541 tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt tccaggacaa 601 gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg cttacccgat 661 cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc cgccaaaaac 721 ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga gcgcgctgat 781 gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg ggggttatgc 841 gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata acgctggcgc 901 gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga atgcagacac 961 cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg 1021 cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa cggtactgcc 1081 gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc 1141 cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa aactatctgc tgactgatga 1201 aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga agtcttacga 1261 ggaagagttg gcgaaagatc cacgtattgc cgccactatg gaaaacgccc agaaaggtga 1321 aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtgcgta ctgcggtgat 1381 caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc agactaatgg 1441 gatcgaggaa aacctgtact tccaatccaa tatgcccgta aggtcattta aagttaagct 1501 agtgactcgg tctggcgatg cggagcacat gctgcaactg cgtagggggc tgtggaaaac 1561 ccatgaaatc gtaaatcagg gcattgcgta ctacatgaac aagttggcgc tgatgcgtca 1621 agaaccgtat gctggtaaaa gccgtgaggt tgttcgtttg gagctgctgc atagcctgcg 1681 tgcacagcag aaacgtaata actggaccgg cgacgccggt acggatgatg aaattctgaa 1741 cctgagccgt cgcctgtacg agttgctcgt gccgagcgcg atcggcgaga agggcgatgc 1801 gcagatgtta tctcgcaagt ttctgtcgcc gctggttgat ccgaattctg aaggtggtaa 1861 aggcacggcg aagtccggcc gtaagccgcg ttggatgaaa atgcgggagg agggtcatcc 1921 ggattgggaa gctgaacgcg agaaggacga agcaaagaaa gcagcggacc cgactgcgtc 1981 tattctgaat gatttggaag cgtttggtct gcgtccgctg ttcccgctgt tcaccgacga 2041 gcaaaaaggc atccagtggc tgccgaaaca aaaacgtcag ttcgtgcgta cctgggatcg 2101 tgatatgttt cagcaggctc tggagcgtat gctgtcctgg gagtcctgga accgccgtgt 2161 cgccgaggaa tatcagaagc tgcaggctca acgtgacgag ttgtacgcaa aatatctggc 2221 cgatggtggt gcctggctgg aagcgttaca aagctttgag aagcagcgtg aagttgaact 2281 ggctgaggag tcttttgccg ctaaatccga atatctgatc acccgacgac aaattcgcgg 2341 ttggaaacag gtgtacgaaa agtggtcaca actgccagag cacgccgccc aagaacaatt 2401 ctggcaagtt gtagcggacg tacagacgtc tctgcctggt gcgtttggtg acccaaaagt 2461 ttaccagttt ctgagccagc cggagcatca ccacatctgg cgcggttacc cgaaccgact 2521 attccactat tctgattaca acggtgttcg caagaagctg caaagagcgc gtcacgatgc 2581 gacttttacc ttgccggacc cggttgaaca cccgctttgg atcagattcg atgcgcgtgg 2641 tggtaatatt catgattatg agatcagcca gaatggcaag caatatcaag ttaccttctc 2701 ccgcctcctg tggcctgaaa atgaaacctg ggtggagcgt gaaaacgtta ccgttgcaat 2761 cggggcaagc cagcagctta agcgtcagat tcggttggac ggctacgccg ataaaaagca 2821 gaaggtacgt tatcgtgact actctagcgg catcgagctg accggtgttc tgggtggtgc 2881 gaagatccag ttcgaccgtc gtcacctgcg caaagcaagt aatcgtttag ccgacggtga 2941 aaccggccct gtgtacctga acgtggtggt cgatatcgag ccgtttctgg ctatgcgcaa 3001 cggtcgtcta caaacgccga ttggccaagt gctgcaagtc gtgaccaagg actggccgaa 3061 ggtgacgggt tataaaccgg ctgagctgat cagctggatt caaaacagcc cgctggcggt 3121 gggtacaggc gtcaacacca ttgaggcggg tatgcgcgtt atgagtgttg atctgggtca 3181 aagaagcgca gctgctgtga gcattttcga ggtcatgcgc cagaaaccgg cggaacagga 3241 aaccaaactc ttctatccga ttgctgtgac cggtctgtat gcggtgcatc gtcgtagttt 3301 gctgctgcgt cttccaggcg agaaaattag cgacgaaatc gaacagcaac gtaaaatcag 3361 agcccatgct cgtagcttgg tgcgctacca gatccgtctg cttgcggacg tactgcgtct 3421 ccacacccgt ggcaccgcgg aacagagacg tgcgaaactg gatgaattgc tggctaccct 3481 gcaaacgaaa caagagttag atcagaagtt atggcaaact gagttggaaa agctgttcga 3541 ctacattcac gaaccggcgg agcgctggca gcaggcgctg gtggcggcgc accgtacctt 3601 ggagccggtt atcggtcagg cagttcgtca ttggcgtaag tccctccgca ttgatcgtaa 3661 gggcttggcg ggcatgagca tgtggaacat tgaagagttg gaggaaaccc ggaagttgtt 3721 gatcgcatgg agcaaacatt ccagggtccc cggcgagccg aaccgtctgg acaaagaaga 3781 aacgtttgca ccgcagcagc tccaacatat ccaaaacgtc aaagacgacc gcttgaagca 3841 gatggcaaat ctgctcgtga tgaccgcctt gggctacaag tacgatgagg ccgagaaaca 3901 atggaaagag gcgtatccgg cgtgtcagat gattctgttc gaagatctaa gccgctaccg 3961 ctttgcgctg gaccgaccgc gtcgcgagaa caaccggtta atgaaatggg cacacagaag 4021 tatcccgcgt ctggtttatc tgcaaggtga attgttcggc atccaggtgg gtgtggttta 4081 ttcggcgtat actagccgtt tccatgctaa aactggtgcg ccaggcattc gctgccacgc 4141 gctgaaagaa gaagacctgc aaccgaacag ctacgtggtg aagcaactga ttaaagacgg 4201 ctttatccgt gaggaccaga ccggttccct taagccgggt cagattgtac cgtggtctgg 4261 cggtgaactg ttcgttacct tagccgaccg ttccggcagc cgtttggccg tgatccacgc 4321 cgacatcaat gcagcgcaga atctgcaaaa gagattctgg caacagaaca ccgagatctt 4381 tcgtgtgccg tgcaaagtga ctacgtccgg tctgataccg gcatacgaca agatgaaaaa 4441 gttattcggc aaaggctatt tcgcaaagat caaccagacg gacaccagcg aagtgtacgt 4501 ttgggaacac agcgctaaga tgaagggcaa aacgaccccg gcggacccag cagaagaagg 4561 agtttttgat gagtccctga ccgacgagat ggaagagctt gaagacagcc aagagggtta 4621 caagaccctg ttccgtgatc cgtcgggctt tttttggtcg agcgatcgct ggctgccgca 4681 gaaagagttc tggttttggg ttaaacgccg tatcgagaag aaactgcgcg agcagctcca 4741 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt 4801 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt 4861 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt 4921 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 4981 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 5041 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 5101 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 5161 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 5221 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 5281 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 5341 aacaaactag taacgtttac aatttcaggt ggcacttttc ggggaaatgt gcgcggaacc 5401 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa ttaattctta 5461 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc 5521 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag 5581 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat 5641 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga 5701 atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc 5761 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc 5821 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg 5881 caaccggcgc aggaacactg ccagcgcatc aacaatgttt tcacctgaat caggatattc 5941 ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc 6001 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag 6061 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa 6121 ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt 6181 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct 6241 agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta 6301 agcagacagt tttattgttc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 6361 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 6421 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 6481 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 6541 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 6601 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 6661 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 6721 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 6781 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 6841 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 6901 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 6961 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 7021 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 7081 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 7141 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt 7201 gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 7261 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg 7321 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 7381 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 7441 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc 7501 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata 7561 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg 7621 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg 7681 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta 7741 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca 7801 gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg 7861 gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt 7921 catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt 7981 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac 8041 gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat ggcctgcttc 8101 tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt 8161 ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg 8221 aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc 8281 ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag 8341 gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt 8401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 8461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt 8521 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag 8581 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg 8641 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc 8701 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc 8761 aaccagcatc gcagtgggaa cgataccctc attcagcatt tgcatggttt gttgaaaacc 8821 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag 8881 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa 8941 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc 9001 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc 9061 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt 9121 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc 9181 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga 9241 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc 9301 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag 9361 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt 9421 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt 9481 tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc 9541 gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact 9601 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga 9661 atggtgcatg //
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇:pGD-γb
- 下一篇:没有了