| 质粒名称: | c-Myc-5-HT2A |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 67944 |
| 存储实验室: | Javier Gonzalez-Maeso |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 4989 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | HTR2A / 5HT2A |
| 物种来源: | H. sapiens (human) |
| 启动子: | CMV |
| 标签/融合蛋白: | c-Myc (N terminal on insert) |
| 克隆方法: | 未知 |
| 5’测序引物 | CMV-F |
| 3’测序引物 | BGH Reverse |
|
文献引用方法 |
|
| 材料和方法部分: |
c-Myc-5-HT2A was a gift from Javier Gonzalez-Maeso (Addgene plasmid # 67944 ; http://n2t.net/addgene:67944 ; RRID:Addgene_67944)
|
| 参考文献部分: | Identification of a serotonin/glutamate receptor complex implicated in psychosis. Gonzalez-Maeso J, Ang RL, Yuen T, Chan P, Weisstaub NV, Lopez-Gimenez JF, Zhou M, Okawa Y, Callado LF, Milligan G, Gingrich JA, Filizola M, Meana JJ, Sealfon SC. Nature. 2008 Mar 6;452(7183):93-7. doi: 10.1038/nature06612. Epub 2008 Feb 24. |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS c-Myc-5-HT2A 6840 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A.
ACCESSION .
VERSION .
KEYWORDS c-Myc-5-HT2A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6840)
AUTHORS Gonzalez-Maeso J, Ang RL, Yuen T, Chan P, Weisstaub NV,
Lopez-Gimenez JF, Zhou M, Okawa Y, Callado LF, Milligan G, Gingrich
JA, Filizola M, Meana JJ, Sealfon SC
TITLE Identification of a serotonin/glutamate receptor complex implicated
in psychosis.
JOURNAL Nature. 2008 Mar 6;452(7183):93-7. doi: 10.1038/nature06612. Epub
2008 Feb 24.
PUBMED 18297054
REFERENCE 2 (bases 1 to 6840)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6840
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(67..86)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 258..637
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 638..841
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 792..812
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 886..905
/label=T7
/note="T7 promoter, forward primer"
promoter 886..904
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 939..968
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
promoter complement(2416..2434)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(2417..2434)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(2454..2471)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2460..2684
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2730..3158
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2817..2836)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3027..3048
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3167..3187)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3172..3501
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3352..3487
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3414..3433
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3568..4362
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3622..3641)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4232..4251
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 4536..4657
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4573..4592)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4627..4646
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4706..4722)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4706..4722)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4719..4741)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4730..4746
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4754..4784)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4799..4820
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4937..4954)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5108..5696)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5188..5207)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5867..6727)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6490..6509
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6728..6832)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
61 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
121 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
181 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
241 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
301 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
361 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
421 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
481 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
541 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
601 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
661 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
721 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
781 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
841 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
901 tagggagacc caagcttggt accgagctcg gatccatgga acaaaaactt atttctgaag
961 aagatctgga tattctttgt gaagaaaata cttctttgag ctcaactacg aactccctaa
1021 tgcaattaaa tgatgacacc aggctctaca gtaatgactt taactccgga gaagctaaca
1081 cttctgatgc atttaactgg acagtcgact ctgaaaatcg aaccaacctt tcctgtgaag
1141 ggtgcctctc accgtcgtgt ctctccttac ttcatctcca ggaaaaaaac tggtctgctt
1201 tactgacagc cgtagtgatt attctaacta ttgctggaaa catactcgtc atcatggcag
1261 tgtccctaga gaaaaagctg cagaatgcca ccaactattt cctgatgtca cttgccatag
1321 ctgatatgct gctgggtttc cttgtcatgc ccgtgtccat gttaaccatc ctgtatgggt
1381 accggtggcc tctgccgagc aagctttgtg cagtctggat ttacctggac gtgctcttct
1441 ccacggcctc catcatgcac ctctgcgcca tctcgctgga ccgctacgtc gccatccaga
1501 atcccatcca ccacagccgc ttcaactcca gaactaaggc atttctgaaa atcattgctg
1561 tttggaccat atcagtaggt atatccatgc caataccagt ctttgggcta caggacgatt
1621 cgaaggtctt taaggagggg agttgcttac tcgccgatga taactttgtc ctgatcggct
1681 cttttgtgtc atttttcatt cccttaacca tcatggtgat cacctacttt ctaactatca
1741 agtcactcca gaaagaagct actttgtgtg taagtgatct tggcacacgg gccaaattag
1801 cttctttcag cttcctccct cagagttcct tgtcttcaga aaagctcttc cagcggtcga
1861 tccataggga gccagggtcc tacacaggca ggaggactat gcagtccatc agcaatgagc
1921 aaaaggcatg caaggtgctg ggcatcgtct tcttcctgtt tgtggtgatg tggtgccctt
1981 tcttcatcac aaacatcatg gccgtcatct gcaaagagtc ctgcaatgag gatgtcattg
2041 gggccctgct caatgtgttt gtttggatcg gttatctctc ttcagcagtc aacccactag
2101 tctacacact gttcaacaag acctataggt cagccttttc acggtatatt cagtgtcagt
2161 acaaggaaaa caaaaaacca ttgcagttaa ttttagtgaa cacaataccg gctttggcct
2221 acaagtctag ccaacttcaa atgggacaaa aaaagaattc aaagcaagat gccaagacaa
2281 cagataatga ctgctcaatg gttgctctag gaaagcagca ttctgaagag gcttctaaag
2341 acaatagcga cggagtgaat gaaaaggtga gctgtgtgtg agcggccgct cgagcatgca
2401 tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac
2461 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
2521 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
2581 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
2641 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
2701 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
2761 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
2821 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
2881 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
2941 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
3001 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
3061 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
3121 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
3181 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
3241 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
3301 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
3361 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
3421 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
3481 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
3541 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
3601 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
3661 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
3721 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
3781 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
3841 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
3901 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
3961 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
4021 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
4081 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
4141 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
4201 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
4261 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
4321 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
4381 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
4441 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
4501 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
4561 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
4621 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
4681 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
4741 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
4801 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
4861 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
4921 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
4981 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
5041 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
5101 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
5161 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
5221 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
5281 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
5341 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
5401 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
5461 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
5521 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
5581 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
5641 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
5701 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
5761 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
5821 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
5881 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
5941 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
6001 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
6061 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
6121 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
6181 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
6241 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
6301 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
6361 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
6421 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
6481 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
6541 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
6601 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
6661 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
6721 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
6781 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
//
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