| 质粒名称: | pHR_Gal4UAS_Blinatumomab_PGK_mCherry |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 85437 |
| 存储实验室: | Wendell Lim |
|
载体骨架基本信息 |
|
| 载体名称: | pHR SIN |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Blinatumomab |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR_Gal4UAS_Blinatumomab_PGK_mCherry was a gift from Wendell Lim (Addgene plasmid # 85437 ; http://n2t.net/addgene:85437 ; RRID:Addgene_85437) |
| 参考文献部分: | Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors. Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, Walker WJ, McNally KA, Lim WA. Cell. 2016 Oct 6;167(2):419-432.e16. doi: 10.1016/j.cell.2016.09.011. Epub 2016 Sep 29. 10.1016/j.cell.2016.09.011 PubMed 27693353 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR_Gal4UAS_Blinatumomab_PGK_mCherry 11707 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION [Edit] Lentiviral vector for Gal4 inducible Blinatumomab and
constitutive mCherry expression.
ACCESSION .
VERSION .
KEYWORDS pHR_Gal4UAS_Blinatumomab_PGK_mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11707)
AUTHORS Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH,
Walker WJ, McNally KA, Lim WA
TITLE Engineering T Cells with Customized Therapeutic Response Programs
Using Synthetic Notch Receptors.
JOURNAL Cell. 2016 Oct 6;167(2):419-432.e16. doi:
10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
PUBMED 27693353
REFERENCE 2 (bases 1 to 11707)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11707
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 166..190
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 271..280
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 343..372
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1867..1884
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 1885..1911
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
promoter 2037..2536
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(2072..2095)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 2469..2488
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 2557..3267
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(2702..2720)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 2970..2989
/label=mCherry-F
/note="mCherry, forward primer"
misc_feature 3344..3932
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3397..3417)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3815..3826)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(3935..3951)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3936..3952)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 4042..4275
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(4300..4318)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4301..4318)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4608..4627)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(4841..4860)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4960..4982
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(5020..5038)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5106..5210
/gene="bla"
/label=AmpR promoter
CDS 5211..6071
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5429..5448)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6242..6830
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6731..6750
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6984..7001
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(7071..7091)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7076..7405
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7256..7391
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7318..7337
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 8539..8604
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 8734..8754
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 9179..9313
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9216..9235)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9270..9289
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 9481..10114
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 10161..10286
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 10783..11016
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 11201..11245
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 11530..11647
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 ggagcactgt cctccgaacg tcggagcact gtcctccgaa cgtcggagca ctgtcctccg
61 aacgtcggag cactgtcctc cgaacggagc atgtcctccg aacgtcggag cactgtcctc
121 cgaacgacta gttaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
181 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
241 ccgatccagc ctctcgacat tcgttggatc gccgccatgg acatgagggt tttggcccag
301 ctgctggggc tgctgttgct gtgtttcccg ggggccagat gcgagcagaa gctgatcagc
361 gaggaggacc tggatattca actcacacag tcccccgcgt ctcttgccgt atctttgggc
421 caacgggcga ccatttcctg caaagcgtcc cagagtgttg attacgatgg ggactcttac
481 ctcaattggt accagcagat tcccggacag ccgcccaagt tgttgatata tgacgcttca
541 aacttggtca gtggcattcc tcccagattt tctgggagcg gaagtggtac cgattttaca
601 cttaacattc atcctgtgga aaaagtggat gccgctactt atcattgtca gcagagtacg
661 gaagacccct ggacgttcgg tggcggtacg aaactggaga tcaaaggcgg cggtgggagc
721 ggtgggggtg gatcaggcgg cggcggaagc caagttcaac tgcagcagag tggtgcagag
781 ctggtcagac ctggcagctc agttaagata tcatgtaaag caagtggata cgcgttttca
841 agctattgga tgaactgggt caagcaaagg cctggccagg gactggagtg gataggacaa
901 atttggcccg gggacggtga taccaactac aacggcaagt ttaagggaaa agccaccctg
961 acggctgatg aatcctcttc tactgcatat atgcaactca gctctttggc aagcgaagac
1021 agcgccgtgt acttctgcgc caggagggaa actacaactg tgggtcgata ttactacgcg
1081 atggactact ggggacaggg gacgactgtc actgtttcat caggtggggg aggatcagac
1141 atcaagctgc aacaatctgg cgctgagttg gcgaggccgg gggcgagtgt taagatgagt
1201 tgtaagacta gtggttatac tttcaccagg tacaccatgc actgggtgaa gcagcgcccg
1261 ggacagggtc ttgaatggat aggttatatc aatccgtccc gagggtatac taactacaac
1321 caaaaattca aagacaaagc gactctgact acggacaaat cctctagcac agcatacatg
1381 cagctttcca gcctcactag tgaagactca gcagtttact attgtgctag gtactatgat
1441 gatcactatt gcctcgatta ctggggacaa gggacgaccc tcacggtgtc aagtgtggag
1501 ggtggctccg gaggttccgg tggatccggc gggagcggtg gagtggatga tatccaactt
1561 actcaatccc cggccataat gagtgcaagt ccaggagaaa aggtgacaat gacttgccga
1621 gcgtcatcat ccgtatctta catgaactgg taccagcaga agagtggcac gtctcctaag
1681 cgatggatat acgacacatc caaggtcgca agcggagtgc cgtaccgatt cagcggttct
1741 gggtctggga ctagttactc cttgactatc tcatccatgg aagccgaaga cgccgcgaca
1801 tactattgtc aacaatggtc ctccaacccc cttacgttcg gtgctggaac aaagttggag
1861 ctgaaacatc accaccatca ccactaccca tacgatgttc cagattacgc ttgagatcct
1921 tgacttgcgg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc gcgactctag
1981 agtcgacctg catctaggcg ccggaattag atctctcgag gttaacgaat tctaccgggt
2041 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
2101 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
2161 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
2221 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
2281 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
2341 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
2401 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
2461 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
2521 atctccgggc ctttcgacct gcagcccaag cttaccatgg tgagcaaggg cgaggaggat
2581 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
2641 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
2701 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
2761 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
2821 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
2881 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
2941 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
3001 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
3061 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
3121 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
3181 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
3241 ggcggcatgg acgagctgta caagtaaagc ggccgcgact ctagagtcga cctgcaggca
3301 tgcaagcttg caggcatgca agcttgatat caagcttatc gataatcaac ctctggatta
3361 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
3421 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
3481 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
3541 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
3601 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
3661 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
3721 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
3781 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
3841 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
3901 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagggaatta
3961 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
4021 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
4081 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
4141 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
4201 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
4261 ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg
4321 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta
4381 caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac
4441 tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca
4501 cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg
4561 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
4621 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
4681 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
4741 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
4801 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
4861 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
4921 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
4981 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
5041 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
5101 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
5161 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
5221 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
5281 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
5341 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
5401 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
5461 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
5521 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
5581 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
5641 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
5701 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
5761 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
5821 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
5881 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
5941 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
6001 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
6061 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
6121 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
6181 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
6241 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
6301 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
6361 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
6421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
6481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
6541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
6601 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
6661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
6721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
6781 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
6841 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
6901 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
6961 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
7021 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
7081 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
7141 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
7201 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
7261 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
7321 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
7381 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
7441 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
7501 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
7561 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
7621 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
7681 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
7741 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
7801 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
7861 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
7921 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
7981 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
8041 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
8101 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
8161 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
8221 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
8281 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
8341 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
8401 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
8461 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
8521 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
8581 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
8641 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
8701 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
8761 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
8821 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
8881 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
8941 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
9001 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
9061 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
9121 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
9181 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
9241 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
9301 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
9361 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
9421 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
9481 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
9541 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
9601 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
9661 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
9721 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
9781 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
9841 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
9901 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
9961 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
10021 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
10081 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
10141 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
10201 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
10261 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
10321 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
10381 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
10441 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
10501 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
10561 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
10621 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
10681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
10741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
10801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
10861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
10921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
10981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
11041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
11101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
11161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
11221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
11281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
11341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
11401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
11461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
11521 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
11581 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
11641 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
11701 cgaattc
//
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