| 出品公司: | Clontech |
|---|---|
| 载体名称: | pBI-CMV2 |
| 质粒类型: | 哺乳细胞表达载体 ； 双向表达载体；荧光报告载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | 弱启动子minCMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3822 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素（Ampicillin ） |
| 筛选标记: | -- |
| 备注: | pBI-CMV2表达绿色荧光蛋白AcGFP1 |
| 产品目录号: | #631631 |
| 稳定性: | -- |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pBI-CMV2载体质粒基本信息
pBI-CMV2质粒图谱载体图谱和pBI-CMV2载体序列质粒序列多克隆位点信息
pBI-CMV2质粒载体简介
双向启动子载体pBI-CMV2表达绿色荧光蛋白AcGFP1
The pBI bidirectional vectors are designed to constitutively express a protein of interest and a reporter protein (or two proteins of interest). Expression is driven by constitutively active bidirectional human cytomegalovirus promoters (PminCMV). The gene(s) of interest must contain an initiation codon and a stop codon.
eatures Constitutively express a protein of interest and a reporter protein (or two proteins of interest)
pBI-CMV2 allows you to express a protein of interest and AcGFP1, a monomeric green fluorescent protein derived from Aequorea coerulescens.
pBI-CMV2质粒序列载体序列
LOCUS pBI-CMV2 3822 bp DNA SYN
DEFINITION pBI-CMV2
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3822
/organism="pBI-CMV2"
/mol_type="other DNA"
promoter 4..568
/label="CMV_immearly_promoter"
misc_feature 71..358
/label="CAG_enhancer"
misc_feature 525..545
/label="CMV_fwd_primer"
promoter 526..595
/label="CMV_promoter"
gene complement(1799..2659)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(1799..2659)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(2701..2729)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS 2969..3520
/label="ORF frame 2"
/translation="MADYDPLDCSLRRSGPSLVQLIHAVGDGGGGHEAEVDHVIALLV
GVLGQGALGGQVVVIGQQHRAIADGGILLVVVGQLHAAILDVVADLEVHLDAILGLVG
HDVHIVGVVVVLHLIAQDVAILLEIGAGQLDAIHQGIALELHLGARLVVAVILEEDGA
LLDVALRHGALEEVVLLHVIGVA*"
CDS complement(3017..3736)
/label="ORF frame 3"
/translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MIYFGFVTAAAITHGMDELYK*"
gene complement(3020..3733)
/label="AcGFP1"
/gene="AcGFP1"
/translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MIYFGFVTAAAITHGMDELYK*"
misc_feature complement(3756..3780)
/label="LNCX_primer"
/translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MIYFGFVTAAAITHGMDELYK*"
misc_feature complement(3763..3782)
/label="pCEP_fwd_primer"
/translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MIYFGFVTAAAITHGMDELYK*"
ORIGIN
1 ctcgagtagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
601 gggatcctct agtcagctga cgcgtgctag cgcggccgca tcgataagct tgtcgacgat
661 atctccagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
721 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
841 aagcattttt ttcactgccc cgagcttcct cgctcactga ctcgctgcgc tcggtcgttc
901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
1021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
1081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
1141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
1201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
1261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
1321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
1381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
1441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
1501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
1561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
1621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
1681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
1741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
1801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
1861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
1921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
1981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
2041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
2101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
2161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
2221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
2281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
2341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
2401 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
2461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
2521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
2581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
2641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
2701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
2761 cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag tgaaaaaaat gctttatttg
2821 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
2881 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
2941 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag actgcagcct
3001 caggagatct gggccctcac ttgtacagct catccatgcc gtgggtgatg gcggcggcgg
3061 tcacgaagcc gaagtagatc atgtgatcgc gcttctcgtt ggggtccttg gacagggcgc
3121 tctgggtgga caggtagtgg ttatcgggca gcagcacagg gccatcgccg atgggggtat
3181 tctgctggta gtggtcggcc agctgcacgc tgccatcctc gatgttgtgg cggatcttga
3241 agttcacctt gatgccattc ttggccttgt cggtcatgat gtacacattg tgggcgttgt
3301 agttgtactc catcttattg cccaggatgt tgccatcctc cttgaaatcg gtgccggtca
3361 gctcgatgcg attcaccagg gtatcgccct cgaacttcac ctcggcgcgc gacttgtagt
3421 tgccgtcatc ctcgaagaag atggtgcgct cctggatgta gccctcaggc atggcgctct
3481 tgaagaagtc gtgctgcttc atgtgatcgg ggtagcgtga gaagcactgc acgccgtagc
3541 tcagggtggt caccagggtg ggccagggca caggcagctt gccggtggtg cagatgaact
3601 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccgctcacg ctgaacttgt
3661 ggccattcac atcgccattc agctcgatca ggatgggcac gatgccggtg aacagctcgg
3721 cgcccttgct caccatggtg gcgaattctc caggcgatct gacggttcac taaacgagct
3781 ctgcttatat aggcctccca ccgtacacgc cacctcgaca ta
//
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pBI-CMV2质粒载体应用举例
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