| 出品人: | Lynch,M.D. and Gill,R.T. |
|---|---|
| 载体名称: | pBTB-1 |
| 质粒类型: | 广宿主载体；克隆载体；原核表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | araBAD |
| 载体大小: | 3784 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
pBTB-1载体质粒基本信息
pBTB-1质粒图谱载体图谱和pBTB-1载体序列质粒序列多克隆位点信息
pBTB-1质粒载体简介
pBTB-1质粒序列载体序列
LOCUS DQ058729 3784 bp DNA circular SYN 08-MAY-2006
DEFINITION Broad host range vector pBTB-1, complete sequence.
ACCESSION DQ058729
VERSION DQ058729.1 GI:69048308
AUTHORS Lynch,M.D. and Gill,R.T.
TITLE Direct Submission
JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering,
University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
80309, USA
FEATURES Location/Qualifiers
source 1..3784
/organism="Broad host range vector pBTB-1"
/mol_type="other DNA"
/db_xref="taxon:334961"
regulatory 60..96
/regulatory_class="terminator"
/note="SoxR terminator"
gene 872..1534
/gene="rep"
/note="rep gene from pBBR1, GenBank Accession Number
X66730"
CDS 872..1534
/gene="rep"
/note="required for replication"
/codon_start=1
/transl_table=11
/product="Rep"
/protein_id="AAY99717.1"
/db_xref="GI:69048309"
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
RR"
gene 1631..2491
/gene="bla"
/note="beta-lactam resistance"
CDS 1631..2491
/gene="bla"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AAY99718.1"
/db_xref="GI:69048310"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 2507..2538
/regulatory_class="terminator"
/note="TonB terminator"
gene complement(2566..3495)
/gene="araC"
CDS complement(2566..3495)
/gene="araC"
/note="regulator"
/codon_start=1
/transl_table=11
/product="AraC"
/protein_id="AAY99719.1"
/db_xref="GI:69048311"
/translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
regulatory 3503..3770
/regulatory_class="promoter"
/note="pBAD promoter"
ORIGIN
1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
1621 ggaagctaaa atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
1681 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
1741 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
1801 tttacgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
1861 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
1921 gaatgacttg gttgagtact caccagtcac agaaaagcat ctcacggatg gcatgacagt
1981 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
2041 ggcaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
2101 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
2161 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
2221 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggatc
2281 acttctgcgc tcggccctcc cggctggctg gtttattgct gataaatctg gagccggtga
2341 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgcatcgt
2401 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
2461 gataggtgcc tcactgatta agcattggta atgaggatcc ccctcaagtc aaaagcctcc
2521 ggtcggaggc ttttgacttt ctgctatgga ggtcaggtat gatttttatg acaacttgac
2581 ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt
2641 gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac
2701 ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc
2761 ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga
2821 cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg
2881 atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc
2941 gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc
3001 cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg
3061 cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt
3121 aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg
3181 agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc
3241 cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag
3301 attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt
3361 ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag
3421 gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa
3481 ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac
3541 caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac
3601 aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca
3661 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg
3721 acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagagaatt
3781 cgtc
//
