| 质粒名称: | WNK2 WT |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 24569 |
| 存储实验室: | Joseph Costello |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1/V5–His–TOPO |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 5523 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达 |
| 筛选标记: | 新霉素（G418筛选） |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | WNK2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | V5（插入件上的C端子）His6（插入件上的C端子） |
| 克隆方法: | TOPO Cloning |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | CMV-F, T7 |
| 3’测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | WNK2 WT was a gift from Joseph Costello (Addgene plasmid # 24569 ; http://n2t.net/addgene:24569 ; RRID:Addgene_24569) |
| 参考文献部分: | Epigenome scans and cancer genome sequencing converge on WNK2, a kinase-independent suppressor of cell growth. Hong C, Moorefield KS, Jun P, Aldape KD, Kharbanda S, Phillips HS, Costello JF. Proc Natl Acad Sci U S A. 2007 Jun 26. 104(26):10974-9. 10.1073/pnas.0700683104 PubMed 17578925 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS WNK2 WT 12100 bp ds-DNA circular SYN 26-JUN-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS WNK2 WT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12100)
AUTHORS Hong C, Moorefield KS, Jun P, Aldape KD, Kharbanda S, Phillips HS,
Costello JF
TITLE Epigenome scans and cancer genome sequencing converge on WNK2, a
kinase-independent suppressor of cell growth.
JOURNAL Proc Natl Acad Sci U S A. 2007 Jun 26. 104(26):10974-9.
PUBMED 17578925
REFERENCE 2 (bases 1 to 12100)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 26, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..12100
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 140..519
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 520..723
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 674..694
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 768..787
/label=T7
/note="T7 promoter, forward primer"
promoter 768..786
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 816..825
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 7503..7544
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 7554..7571
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(7594..7611)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7600..7824
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7870..8298
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7957..7976)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8167..8188
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(8307..8327)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8312..8641
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8492..8627
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8554..8573
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 8708..9502
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(8762..8781)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 9372..9391
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 9678..9799
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9715..9734)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9769..9788
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(9848..9864)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9848..9864)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9861..9883)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9872..9888
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9896..9926)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9941..9962
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(10079..10096)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10250..10838)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10330..10349)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11009..11869)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11632..11651
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11870..11974)
/gene="bla"
/label=AmpR promoter
primer_bind complement(12049..12068)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
61 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
121 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
181 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
241 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
301 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
361 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
421 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
481 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
541 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
601 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
661 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
721 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
781 tatagggaga cccaagctgg ctagttaagc ttgccgccac catggacggc gatggcggcc
841 gccgagacgt ccccggcacg ctgatggagc ccgggcgcgg cgcggggccc gcgggcatgg
901 cggagcctcg ggcgaaggcg gcgcggccgg ggccccagcg ctttctgcgg cgcagcgtgg
961 tagagtcgga ccaggaggag ccgccgggct tggaggcagc cgaggcgccg ggcccgcagc
1021 ccccgcagcc cctgcagcgc cgggtgcttc tgctctgcaa gacgcgccgc ctcatcgcgg
1081 agcgcgctcg cggacgcccc gccgcccccg cgcccgcagc gctggtagcg cagccgggag
1141 cccccggagc ccccgcggac gccggccccg agcccgtggg cacgcaggag cccggcccgg
1201 accccatcgc agccgctgtc gaaaccgcgc ctgcccccga cggcggcccc agggaggagg
1261 cggcggcgac cgtgaggaag gaggatgagg gggcggccga ggcgaagcct gagcccgggc
1321 gcactcgccg ggacgagccc gaagaggagg aggacgacga ggacgacctc aaggccgtgg
1381 ccacctctct ggacggccgc ttcctcaagt tcgacatcga gctgggccgc ggttccttca
1441 agacggtcta caaggggctg gacacggaga cctgggtgga ggtggcctgg tgtgagctgc
1501 aggaccggaa gctcaccaag ctggagcggc agcggttcaa ggaagaggct gagatgctga
1561 aaggcctgca gcaccccaac atcgtgcgct tctacgactt ctgggagtcc agcgccaagg
1621 gcaagcggtg cattgtgctg gtgacggagc tgatgacctc agggacgctg aagacatacc
1681 tgaagcggtt caaggtgatg aagcccaagg ttctccgcag ctggtgccgg cagatcctga
1741 agggcctgct gttcctgcac acaaggacgc cacccatcat ccaccgagac ctgaaatgtg
1801 acaatatttt catcaccgga ccaactgggt ccgtgaagat tggcgacttg ggcctggcca
1861 ctctgaaaag agcgtcattt gccaaaagtg tgataggtac tcccgagttc atggcgcccg
1921 agatgtacga ggagcactac gatgagtccg tggacgtcta tgcctttggg atgtgcatgc
1981 tggagatggc cacctcggag tacccctact cggagtgcca gaatgcggcc cagatctacc
2041 gcaaggtcac ctgtggtatc aagccggcca gctttgagaa agtgcacgat cctgaaatca
2101 aggagattat tggggagtgt atctgcaaaa acaaggagga aaggtacgag atcaaagacc
2161 tgctgagcca cgccttcttc gcagaggaca caggcgtgag ggtggagctc gcggaggagg
2221 accacggcag gaagtccacc atcgccctga ggctctgggt ggaagacccc aagaaactga
2281 agggaaagcc caaggacaat ggagccatag agttcacctt cgacctggag aaggagacgc
2341 cggatgaggt ggcccaagag atgattgagt ctggattctt ccacgagagt gacgtcaaga
2401 tcgtggccaa gtccatccgt gaccgcgtgg ccttgatcca gtggcggcgg gagaggatct
2461 ggcccgcgct gcagcccaag gagcagcagg atgtgggcag cccggacaag gccaggggtc
2521 cgccggtgcc cctgcaggtc caggtgacct accatgcaca ggctgggcag cccgggccac
2581 cagagcccga ggagccggag gccgaccagc acctcctgcc acctacgttg ccgaccagcg
2641 ccacctccct ggcctcggac agcaccttcg acagcggcca gggctctacc gtgtactcag
2701 actcgcagag cagccagcag agcgtgatgc ttggctccct tgccgacgca gcgccgtccc
2761 cggcccagtg tgtgtgcagc ccccctgtga gcgaggggcc cgtcctgccg cagagcctgc
2821 cctcgctggg ggcctaccag cagcccacgg ctgcacctgg cttgccggtg ggctctgtcc
2881 cggcccccgc ctgccctccg tccctccagc agcacttccc ggatccggcc atgagcttcg
2941 cccccgtgct gccgccgccc agcaccccca tgcccacggg cccaggccag ccagcacccc
3001 ccggccagca gcctcctccg ctggcccagc cgacacccct gccgcaggtc ctggccccac
3061 agcccgtggt ccccctccag ccggttcccc cccacctgcc accgtacctg gctccagcct
3121 cccaggtggg ggcccccgct cagctgaagc ccctccagat gccacaggcg cccctgcagc
3181 cgcttgctca agtccctccg cagatgcccc cgattcctgt tgtgcccccc atcacgcccc
3241 tggcgggaat cgacggcctc cctccggccc tcccagacct gccgaccgcg actgtgcctc
3301 ccgtgccacc acctcagtat ttctctccag ccgtgatctt gccgagcctc gctgccccac
3361 tcccccctgc gtccccagcc ttgcctctgc aggctgtgaa gctgccccac ccccctgggg
3421 cgcccctggc catgccctgc cggaccattg tgccaaatgc accggccact atccccctgc
3481 tggccgtagc cccaccgggc gtggctgccc tgtccattca ttctgccgtg gcccagctcc
3541 caggccaacc tgtgtaccca gcggccttcc cacagatggc gcctactgac gtccctcctt
3601 ccccccatca cacggtgcag aatatgaggg ccacccctcc acagccggca ctgcctccac
3661 aacccacact gcccccacaa cccgtgctgc ccccgcaacc cacgctgccc cctcaacctg
3721 tgttgccccc gcaacccaca cggccccctc aacctgtgct gcccccgcaa cccatgctgc
3781 ccccacaacc tgtgctgccc ccgcagccgg cactgcctgt gcgccctgag cccctccagc
3841 cccaccttcc tgaacaagct gctccagctg ctacaccagg gagccagatt ctgcttggcc
3901 acccagctcc ctatgctgtg gacgtcgccg ctcaggtccc caccgtgcct gtgccaccgg
3961 ctgcggtcct ctccccgcct ctgccggaag tgctgctgcc tgccgcccct gagctcctgc
4021 ctcagttccc cagctccctg gccacggtgt ctgcctctgt gcagagtgtg cccacccaga
4081 ctgccacact tctgccacca gcaaacccac cgctgcctgg cgggcccggg atcgccagcc
4141 cttgcccaac tgtccagctg acggtggaac cagtccaaga ggagcaggcc tcacaggaca
4201 agccgcccgg cctcccgcag agctgtgaga gctatggagg ttctgatgtc acttctggaa
4261 aagagctgag tgacagctgt gaaggcgcct ttggaggggg caggctggag ggcagggcag
4321 cccgaaaaca ccaccgcagg tccacgcgtg cgcgctcccg gcaggagagg gccagccggc
4381 cccggcttac catcttgaac gtgtgcaaca ctggggacaa gatggtggag tgccagctgg
4441 agacacacaa ccacaagatg gtgaccttca agttcgactt ggacggggac gcacccgatg
4501 aaattgccac gtatatggtg gagcatgact ttatcctgca ggccgagcgg gaaacgttca
4561 tcgagcagat gaaggatgtc atggacaagg cagaggacat gctcagcgag gacacagacg
4621 ccgaccgtgg ctccgaccca gggaccagcc cgccacacct cagcacctgc ggcctgggca
4681 ccggggagga gagccgacaa tcccaagcca acgcccccgt gtatcagcag aacgtcctgc
4741 acaccgggaa gaggtggttc atcatctgtc cggtggctga gcaccccgcc cccgaggccc
4801 ctgaatcttc gcccccactt cctctaagct ccctgccgcc agaagccagc caagattcag
4861 cgccctataa agaccagctg tcctcgaagg aacaacccag ctttctagcc agtcagcagc
4921 tcctgagcca ggcgggcccc agcaaccctc ctggggcacc cccagcccct ttggccccct
4981 cctcccctcc tgtgactgct ctgccccaag atggagcagc tccagccacc agcaccatgc
5041 cagagccagc gtcaggaact gccagccagg cagggggtcc agggacacct caggggctga
5101 ccagtgagct cgagacgtct cagccactag cggagactca cgaggccccg cttgctgtgc
5161 agcccctcgt ggtgggccta gcaccttgca ctccagctcc agaggctgcc tcaaccaggg
5221 acgccagtgc cccaagggag cccctgccac ctcctgcacc tgagcccagc ccccacagcg
5281 ggaccccaca gcccgccttg ggtcagcctg ctcccctgct tcctgccgca gtgggggccg
5341 tcagcctggc cacctcccag ctcccaagcc cacccctggg gcccaccgtc cccccacagc
5401 caccctcggc cctggagtcg gatggggaag ggccgccccc cagggtgggc tttgtggaca
5461 gcaccatcaa gagcctggac gagaagctgc ggactctgct ctaccaggag cacgtgccca
5521 cctcctcagc ctcagctggg acccctgtgg aggtgggcga cagagacttc accctggagc
5581 ccctgagagg ggaccagccc cgctcagagg tctgcggggg ggacctggcc ctgcccccag
5641 tgcctaagga ggcggtctca gggcgtgtcc agctgcccca gcccttggtg gagaagtcag
5701 aactggcccc cactcgaggg gccgtgatgg agcagggcac gtcctcgtca atgacagcag
5761 agtcgtctcc caggagtatg ctaggctatg acagagatgg aaggcaggtg gcctcagact
5821 cccatgtggt ccccagcgtc ccccaggatg tacctgcttt tgtgagacct gcacgtgtgg
5881 agcccacaga cagggatggt ggagaagctg gagaaagctc ggcagagccc ccgccgagtg
5941 acatgggcac agtggggggc caggctagcc acccccagac actcggcgct cgagctttgg
6001 ggtcccctcg gaaacgtcca gagcagcagg atgtcagctc accagccaag actgtgggcc
6061 gtttctcggt ggtcagcact caggacgagt ggaccctggc ctccccccac agcctgagat
6121 actctgcccc acccgacgtc tacctggacg aggccccctc cagccccgac gtgaagctgg
6181 cagtgcggcg ggcgcagacg gcctcctcca tcgaggtcgg cgtgggcgag cccgtgtcca
6241 gcgactctgg ggacgagggc cctcgggcga gacccccggt gcagaagcag gcgtccctgc
6301 ccgtgagtgg cagcgtggct ggcgacttcg tgaagaaggc caccgccttc ctgcagaggc
6361 cttctcgggc cggctcgctg ggccccgaga cacccagcag ggtgggcatg aaggtcccca
6421 cgatcagcgt gacctccttc cattcccagt cgtcctacat cagcagcgac aatgattcgg
6481 agctcgagga tgctgacata aagaaggagc tgcagagtct gcgggagaag cacctgaagg
6541 agatctcgga gctgcagagc cagcagaagc aggagatcga agctctgtac cgccgcctgg
6601 gcaagccact gccccccaac gtgggcttct tccacacggc accccccact ggccgccgga
6661 gaaaaaccag caagagcaag ctgaaggcag gcaagctgct aaatcccctg gtgcggcagc
6721 tcaaggtcgt ggcctccagc acaggtcact tggctgactc cagcagaggc cctcccgcta
6781 aggaccctgc ccaagccagt gtggggctca ctgcagacag cacgggcctg agcgggaagg
6841 cagtgcagac ccagcagccc tgctccgtcc gggcctccct gtcttcggac atctgctccg
6901 gcttagccag tgatggaggc ggagcgcgtg gccaaggctg gacggtttac cacccaacgt
6961 ctgagagagt gacctataag tctagtagca aacctcgtgc tcgattcctc agtggacccg
7021 tatctgtgtc catctggtct gccctgaagc gtctctgcct aggcaaagaa cacagcagta
7081 ggtcctccac cagcagcctg gccccaggcc ctgagccagg cccccagccc gccctgcacg
7141 tccaggcgca ggtgaacaac agcaacaaca agaagggtac cttcacggac gacctgcaca
7201 agctggtgga cgagtggacg agcaagacgg tgggggccgc gcagctgaag cccacgctca
7261 accagctgaa gcagacccag aagctgcaag acatggaggc ccaggcaggc tgggctgccc
7321 ctggcgaggc gcgggctatg accgcacctc gagcaggagt ggggatgcca cgtctgcccc
7381 cagcgcccgg ccctctgtcc accacggtca ttcccggagc cgccccgacc ctgtccgtgc
7441 ccacaccaga tcctgagagt gagaagcctg acagtgatca tctagagggc ccgcggttcg
7501 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc
7561 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
7621 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
7681 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
7741 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
7801 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
7861 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
7921 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
7981 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
8041 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
8101 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
8161 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
8221 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
8281 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
8341 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
8401 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
8461 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
8521 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
8581 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
8641 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
8701 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
8761 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
8821 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
8881 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
8941 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
9001 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
9061 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
9121 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
9181 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
9241 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
9301 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
9361 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
9421 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
9481 ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga
9541 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
9601 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
9661 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
9721 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
9781 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
9841 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
9901 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
9961 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
10021 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
10081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
10141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
10201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
10261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
10321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
10381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
10441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
10501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
10561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
10621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
10681 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
10741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
10801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
10861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
10921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
10981 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
11041 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
11101 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
11161 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
11221 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
11281 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
11341 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
11401 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
11461 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
11521 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
11581 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
11641 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
11701 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
11761 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
11821 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
11881 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
11941 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
12001 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
12061 tgatgccgca tagttaagcc agtatctgct ccctgcttgt
//
