| 出品公司: | CAMBIA |
|---|---|
| 载体名称: | pCAMBIA390 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 6813 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 6xHis |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | -- |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCambia0390载体 pCambia 0390质粒基本信息
pCambia0390质粒图谱 pCambia 0390载体图谱和pCambia0390载体序列 pCambia 0390质粒序列多克隆位点信息
pCambia0390质粒 pCambia 0390载体简介
pCambia0390质粒序列 pCambia 0390载体序列
LOCUS pCambia0390 6813 bp ds-DNA circular SYN 01-十二月-2013
DEFINITION Agrobacterium binary vector for plant transformation, with a
kanamycin-resistance gene. The MCS is reversed in pCAMBIA0380.
ACCESSION AF234291
VERSION .
KEYWORDS pCAMBIA0390
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT The GenBank record was corrected by inserting a G at position 2718.
FEATURES Location/Qualifiers
source 1..6813
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
CDS 6..23
/codon_start=1
/product="6xHis affinity tag"
/label="6xHis"
/translation="HHHHHH"
terminator 58..310
/label="NOS terminator"
/label="nopaline synthase terminator and poly(A) signal"
misc_feature 332..356
/label="RB T-DNA repeat"
/label="right border repeat from nopaline C58 T-DNA"
CDS 1656..2285
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/label="pVS1 StaA"
CDS 2714..3787
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/label="pVS1 RepA"
misc_feature 4391..4531
/label="bom"
/label="basis of mobility region from pBR322"
rep_origin complement(4717..5305)
/direction=LEFT
/label="ori"
/label="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5392..6186)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label="KanR"
/label="confers resistance to kanamycin"
misc_feature 6611..6635
/label="LB T-DNA repeat"
/label="left border repeat from nopaline C58 T-DNA"
misc_feature 6738..6773
/label="MCS"
/label="multiple cloning site from pUC9"
ORIGIN
1 ctagccacca ccaccaccac cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat
61 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
121 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
181 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
241 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
301 ttactagatc gggaattaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
361 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt
421 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
481 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
541 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
601 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
661 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
721 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
781 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
841 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
901 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
961 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
1021 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
1081 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
1141 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
1201 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
1261 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
1321 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
1381 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
1441 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
1501 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
1561 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
1621 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
1681 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
1741 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
1801 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
1861 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
1921 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
1981 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
2041 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
2101 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
2161 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
2221 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
2281 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
2341 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
2401 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
2461 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
2521 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
2581 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
2641 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
2701 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
2761 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
2821 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
2881 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca
2941 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
3001 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
3061 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
3121 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
3181 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
3241 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
3301 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
3361 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
3421 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg
3481 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
3541 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
3601 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
3661 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
3721 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
3781 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
3841 aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
3901 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
3961 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
4021 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca
4081 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
4141 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
4201 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
4261 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
4321 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
4381 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
4441 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
4501 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
4561 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
4621 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
4681 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
4741 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
4801 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
4861 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
4921 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
4981 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
5041 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
5101 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
5161 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
5221 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
5281 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
5341 tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa
5401 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
5461 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
5521 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
5581 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
5641 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
5701 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
5761 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
5821 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
5881 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
5941 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
6001 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
6061 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
6121 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
6181 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
6241 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
6301 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
6361 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
6421 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
6481 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
6541 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
6601 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
6661 acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcct aggccaccat
6721 gttgggcccg gcgcgccaag cttggctgca ggtcgacgga tccccgggaa ttctaagagg
6781 agtccaccat ggtagatctg actagtgtta acg
//
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